##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936295_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1741669 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.525335181369137 33.0 31.0 34.0 30.0 34.0 2 31.615859270619158 33.0 31.0 34.0 30.0 34.0 3 31.688691134767858 34.0 31.0 34.0 30.0 34.0 4 35.16913202221547 37.0 35.0 37.0 33.0 37.0 5 35.14542085780938 37.0 35.0 37.0 33.0 37.0 6 35.23124083852902 37.0 35.0 37.0 33.0 37.0 7 35.173250485597435 37.0 35.0 37.0 32.0 37.0 8 35.17061795323911 37.0 35.0 37.0 32.0 37.0 9 36.80957518334425 39.0 37.0 39.0 33.0 39.0 10-11 36.72067913019064 39.0 37.0 39.0 33.0 39.0 12-13 36.617077355111675 39.0 37.0 39.0 32.0 39.0 14-15 37.82964702248246 40.0 38.0 41.0 32.5 41.0 16-17 37.77550728640172 40.0 38.0 41.0 32.0 41.0 18-19 37.70985014948305 40.0 38.0 41.0 32.0 41.0 20-21 37.62207026708289 40.0 38.0 41.0 32.0 41.0 22-23 37.45293623530074 40.0 37.5 41.0 32.0 41.0 24-25 37.60349612928748 40.0 38.0 41.0 32.0 41.0 26-27 37.54725524769632 40.0 38.0 41.0 32.0 41.0 28-29 37.40844729968782 40.0 38.0 41.0 32.0 41.0 30-31 37.256172671156236 40.0 37.0 41.0 31.0 41.0 32-33 37.071172249147224 40.0 37.0 41.0 31.0 41.0 34-35 36.79729874046102 40.0 36.5 41.0 30.0 41.0 36-37 36.59282418186234 40.0 36.0 41.0 30.0 41.0 38-39 36.42337263854383 40.0 36.0 41.0 30.0 41.0 40-41 36.23553786626506 40.0 36.0 41.0 29.5 41.0 42-43 36.03657755865207 39.5 35.0 41.0 28.5 41.0 44-45 35.84568881917288 39.0 35.0 41.0 28.0 41.0 46-47 35.56912306528967 39.0 35.0 41.0 27.0 41.0 48-49 35.39102636608908 39.0 35.0 41.0 26.5 41.0 50-51 35.07418573793298 38.5 34.5 40.5 26.0 41.0 52-53 35.09192992468718 39.0 35.0 40.0 26.0 41.0 54-55 35.15657452707719 39.0 35.0 41.0 26.0 41.0 56-57 34.97573333394577 39.0 35.0 41.0 26.0 41.0 58-59 34.71574334732949 38.0 34.5 40.5 25.5 41.0 60-61 34.386234410786436 38.0 34.0 40.0 25.0 41.0 62-63 34.05981819737275 37.0 34.0 40.0 23.5 41.0 64-65 33.70252872388497 37.0 34.0 40.0 23.0 41.0 66-67 33.323742628478776 36.0 33.0 39.0 22.0 41.0 68-69 32.93093435090135 35.5 33.0 39.0 21.5 41.0 70-71 32.51662772891979 35.0 33.0 38.5 20.0 40.0 72-73 32.104475075344396 35.0 32.0 37.0 19.0 40.0 74-75 31.691880030017185 35.0 32.0 37.0 18.0 39.0 76-77 31.299597684749514 35.0 32.0 36.5 14.5 39.0 78-79 30.913680211337514 35.0 31.0 36.0 9.5 38.5 80-81 30.52847900490851 35.0 31.0 35.5 7.0 37.0 82-83 30.1922885462163 34.5 31.0 35.0 4.5 37.0 84-85 29.887710868138548 34.0 31.0 35.0 2.0 36.0 86-87 29.57494391873542 34.0 30.0 35.0 2.0 36.0 88-89 29.25310434990805 34.0 30.0 35.0 2.0 36.0 90-91 28.89598999580288 34.0 29.5 35.0 2.0 35.0 92-93 28.576625351889483 34.0 29.0 35.0 2.0 35.0 94-95 28.181591335667108 34.0 29.0 35.0 2.0 35.0 96-97 27.734611456022932 34.0 29.0 35.0 2.0 35.0 98-99 27.218300951558533 34.0 27.5 35.0 2.0 35.0 100-101 24.00865635203934 29.5 13.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27494431624062443 1101 2 0.3729761348042828 1101 3 0.2219924406423779 1101 4 0.14725905956196073 1101 5 0.31840823868787993 1101 6 0.36515034310221495 1101 7 0.32102765378639475 1101 8 0.35062582245670626 1101 9 0.3426518154908891 1101 10-11 0.43483503523901135 1101 12-13 0.2791859056536339 1101 14-15 0.6096262684794098 1101 16-17 0.47920164137557464 1101 18-19 0.527955118537264 1101 20-21 0.6507546110656932 1101 22-23 0.5770786480984142 1101 24-25 0.5635046960280974 1101 26-27 0.4146534568471054 1101 28-29 0.5085314615559682 1101 30-31 0.48926790317295143 1101 32-33 0.535276629012877 1101 34-35 0.4654948119428042 1101 36-37 0.5243599177274731 1101 38-39 0.6133003478538157 1101 40-41 0.42597631054074014 1101 42-43 0.539151774445827 1101 44-45 0.6703874886114818 1101 46-47 0.5216690446918832 1101 48-49 0.6282704117792264 1101 50-51 0.6441358178495946 1101 52-53 0.720091663949681 1101 54-55 0.6435093386273536 1101 56-57 0.7033977015938362 1101 58-59 0.7243779563616926 1101 60-61 0.48886271744692067 1101 62-63 0.540246231172496 1101 64-65 0.6660891847215709 1101 66-67 0.6659902233024084 1101 68-69 0.6544397570839493 1101 70-71 0.7995508433651821 1101 72-73 0.7874427604029179 1101 74-75 0.677577434224542 1101 76-77 0.6106106270965768 1101 78-79 0.754712439787447 1101 80-81 0.7290192387523113 1101 82-83 0.8552248561858988 1101 84-85 0.9358574496132377 1101 86-87 0.8020892698299882 1101 88-89 0.6211701615817411 1101 90-91 0.6203441292202427 1101 92-93 0.600815986793009 1101 94-95 0.9174993495747259 1101 96-97 0.9979729324246307 1101 98-99 1.1012945745708684 1101 100-101 0.9766713991398959 1102 1 0.3980019599209541 1102 2 0.4020578707364635 1102 3 0.32732828086910004 1102 4 0.362971688609214 1102 5 0.4540884107851966 1102 6 0.3785028097960961 1102 7 0.28474088668387765 1102 8 0.28619826218319133 1102 9 0.4299707890827378 1102 10-11 0.5061446868929096 1102 12-13 0.4641347670871596 1102 14-15 0.6104917299616517 1102 16-17 0.46852876008360056 1102 18-19 0.566695027204922 1102 20-21 0.5192151875368083 1102 22-23 0.6224171644795646 1102 24-25 0.6336250886679835 1102 26-27 0.6098729186655873 1102 28-29 0.5568343336186317 1102 30-31 0.5971169268912249 1102 32-33 0.4070939780417504 1102 34-35 0.43354350079867743 1102 36-37 0.6033969653115534 1102 38-39 0.5193021388187091 1102 40-41 0.39737791211623374 1102 42-43 0.40044334490245603 1102 44-45 0.33103781129395315 1102 46-47 0.4050165879477987 1102 48-49 0.3440580932345014 1102 50-51 0.3057648239327051 1102 52-53 0.45621340152872136 1102 54-55 0.3924538873350869 1102 56-57 0.3646377217679202 1102 58-59 0.443868938064341 1102 60-61 0.5506171253820611 1102 62-63 0.6188964372020749 1102 64-65 0.45570827874036723 1102 66-67 0.5184067392924234 1102 68-69 0.6864768424086733 1102 70-71 0.7625538660473055 1102 72-73 0.6997193615495476 1102 74-75 0.7674619135581118 1102 76-77 0.6960292737172438 1102 78-79 0.6426510460599424 1102 80-81 0.6557839567439601 1102 82-83 0.8124068141016529 1102 84-85 0.8249484320020812 1102 86-87 0.7708408780958749 1102 88-89 0.6121492910081194 1102 90-91 0.6566429047080362 1102 92-93 0.5368516070947287 1102 94-95 0.4836702700689237 1102 96-97 0.5698371141561047 1102 98-99 0.6584000219175259 1102 100-101 0.8999189468560722 1103 1 0.3069164370537827 1103 2 0.2033777481924588 1103 3 0.18933481410857667 1103 4 0.23420438409468858 1103 5 0.13539454281121976 1103 6 0.12629954717019132 1103 7 0.26093891073671927 1103 8 0.20925921634234612 1103 9 0.2779810299272967 1103 10-11 0.3063506603306152 1103 12-13 0.3413948169767522 1103 14-15 0.4156693971325396 1103 16-17 0.44239538614634455 1103 18-19 0.26453436542736597 1103 20-21 0.48597674925728285 1103 22-23 0.5013013717127208 1103 24-25 0.498109460313799 1103 26-27 0.4713827193568534 1103 28-29 0.3393154003705021 1103 30-31 0.4338862016949818 1103 32-33 0.5318882542183871 1103 34-35 0.38059218558068864 1103 36-37 0.2652766528609547 1103 38-39 0.5742788664411975 1103 40-41 0.572012060690021 1103 42-43 0.4862160344304698 1103 44-45 0.6352355341454796 1103 46-47 0.6185177702877525 1103 48-49 0.6236226243672363 1103 50-51 0.5889691482516213 1103 52-53 0.8547767877636474 1103 54-55 0.850966526973508 1103 56-57 0.8502062683634293 1103 58-59 0.9065786349160447 1103 60-61 0.7714353362462774 1103 62-63 0.6721219121765714 1103 64-65 0.6589338785220349 1103 66-67 0.7266593639872738 1103 68-69 0.8248220573848712 1103 70-71 0.8149791190314417 1103 72-73 0.9559299744858905 1103 74-75 1.0063245374708352 1103 76-77 0.8768301447049041 1103 78-79 0.7727259137227378 1103 80-81 0.7168947489034068 1103 82-83 0.7050291708937415 1103 84-85 0.6105615347583253 1103 86-87 0.901164528056686 1103 88-89 0.9335959891927565 1103 90-91 0.8850450234346035 1103 92-93 0.781683342064202 1103 94-95 0.7886638466597589 1103 96-97 0.93987914921194 1103 98-99 0.8827681648034371 1103 100-101 0.8885589979770891 1104 1 0.3087735802519376 1104 2 0.2758636226849056 1104 3 0.34870658409971966 1104 4 0.26384706305955774 1104 5 0.4107279573050491 1104 6 0.3998868173845267 1104 7 0.4485366315589232 1104 8 0.5037942502268535 1104 9 0.5000683824956695 1104 10-11 0.4794486998271381 1104 12-13 0.5505549729003931 1104 14-15 0.6165595368479515 1104 16-17 0.5735793984827353 1104 18-19 0.43263446974247444 1104 20-21 0.6060310313091151 1104 22-23 0.6845498445824418 1104 24-25 0.6508302467112941 1104 26-27 0.7424373045670194 1104 28-29 0.7625888897707043 1104 30-31 0.6842102469252538 1104 32-33 0.7100540507967565 1104 34-35 0.6789184889825108 1104 36-37 0.6842713129774936 1104 38-39 0.6778808410284114 1104 40-41 0.7124919340796438 1104 42-43 0.6976970225834194 1104 44-45 0.6491205440398957 1104 46-47 0.676355368127922 1104 48-49 0.5772053830126183 1104 50-51 0.47242863842046745 1104 52-53 0.557425007126163 1104 54-55 0.8479814915374106 1104 56-57 0.8442576811528895 1104 58-59 0.6978994743065812 1104 60-61 0.5849064275924221 1104 62-63 0.6890832630692145 1104 64-65 0.6661338890251614 1104 66-67 0.7145025953209583 1104 68-69 0.6230081202174134 1104 70-71 0.6965951916748665 1104 72-73 0.8875438987631483 1104 74-75 0.6139845370411514 1104 76-77 0.6024276888836688 1104 78-79 0.6221129132429333 1104 80-81 0.5070644220891722 1104 82-83 0.6262748413391463 1104 84-85 0.43005163198382945 1104 86-87 0.4865585026057069 1104 88-89 0.43542747135057525 1104 90-91 0.44642265130416803 1104 92-93 0.3976652766918072 1104 94-95 0.2798531582993675 1104 96-97 0.2880899182554195 1104 98-99 0.7753796948459488 1104 100-101 1.2262481437150896 1105 1 0.1858892599557116 1105 2 0.2639017947840223 1105 3 0.19876204882454118 1105 4 0.12136881412150302 1105 5 0.16745890121030982 1105 6 0.23211046970766347 1105 7 0.33774632687012485 1105 8 0.2335743625477562 1105 9 0.2635718205355815 1105 10-11 0.3500309441579432 1105 12-13 0.3492647899634278 1105 14-15 0.37487750625925287 1105 16-17 0.3329707166106175 1105 18-19 0.38876051961047864 1105 20-21 0.39334217102224756 1105 22-23 0.4261901316848622 1105 24-25 0.377775473846107 1105 26-27 0.4555567411845658 1105 28-29 0.4162713996364644 1105 30-31 0.607868367772646 1105 32-33 0.6514096705135941 1105 34-35 0.322721127325444 1105 36-37 0.607139013181957 1105 38-39 0.6056539806410441 1105 40-41 0.6015128174181967 1105 42-43 0.6199801207231275 1105 44-45 0.5030695551444353 1105 46-47 0.46289657242750337 1105 48-49 0.4555190621211622 1105 50-51 0.3875880946729069 1105 52-53 0.37737839356648095 1105 54-55 0.5734550675240797 1105 56-57 0.5928300925735996 1105 58-59 0.539096043610428 1105 60-61 0.5338485394263159 1105 62-63 0.21205841409946657 1105 64-65 0.2874371747786526 1105 66-67 0.3427329807138264 1105 68-69 0.6103540461691139 1105 70-71 0.5573105126938742 1105 72-73 0.616948706221315 1105 74-75 0.611966355433804 1105 76-77 0.6702767011477491 1105 78-79 0.6093527411670081 1105 80-81 0.7236155211410562 1105 82-83 0.5851292918518602 1105 84-85 0.44985910947906405 1105 86-87 0.4289223434702585 1105 88-89 0.6145597866066588 1105 90-91 0.5808521376660281 1105 92-93 0.5610705402362122 1105 94-95 0.466339477644766 1105 96-97 0.6323333755437126 1105 98-99 0.6757005012682633 1105 100-101 0.8089077835615655 1106 1 0.17675956452037056 1106 2 0.2420911830620227 1106 3 0.2274234589421269 1106 4 0.2722663419166551 1106 5 0.3267780916814118 1106 6 0.18858075292077103 1106 7 0.1855402538783153 1106 8 0.1842644752534568 1106 9 0.31080526926897534 1106 10-11 0.31322982625161444 1106 12-13 0.30011256101377626 1106 14-15 0.36992432716547086 1106 16-17 0.34208420774209713 1106 18-19 0.28587710671719435 1106 20-21 0.3684962072283753 1106 22-23 0.20015610645952364 1106 24-25 0.22282076964137332 1106 26-27 0.2401937266502543 1106 28-29 0.28593293938848063 1106 30-31 0.41666444518727985 1106 32-33 0.26641371205762 1106 34-35 0.08483965894862422 1106 36-37 0.1302494007126498 1106 38-39 0.24553334436475183 1106 40-41 0.15652433200587978 1106 42-43 0.14025189549474248 1106 44-45 0.2710563324324866 1106 46-47 0.2510668586908267 1106 48-49 0.0911304028462041 1106 50-51 0.29904930347584013 1106 52-53 0.11672511229027549 1106 54-55 0.17374769818795244 1106 56-57 0.18905105827385427 1106 58-59 0.17237911182863286 1106 60-61 0.27931781606618955 1106 62-63 0.13822626781082903 1106 64-65 0.10426887115956163 1106 66-67 0.2622965320871913 1106 68-69 0.22803847021096146 1106 70-71 0.23056712023706893 1106 72-73 0.3725407161650409 1106 74-75 0.21216538241301564 1106 76-77 0.2652817721416625 1106 78-79 0.2157862310783969 1106 80-81 0.22895531586112128 1106 82-83 0.2070155872048467 1106 84-85 0.2705811732592025 1106 86-87 0.15639969557262745 1106 88-89 0.038618602510513966 1106 90-91 0.31764658321193906 1106 92-93 0.19753213031689043 1106 94-95 0.1345025958866195 1106 96-97 0.21772567220829586 1106 98-99 0.14073580758679682 1106 100-101 0.2082203398000324 1107 1 0.023729494733835566 1107 2 0.13493870461374513 1107 3 0.2646514967089608 1107 4 0.035970287934716794 1107 5 0.10018352066335012 1107 6 0.11699824060549702 1107 7 0.09001993368126904 1107 8 0.1488131718872836 1107 9 0.19413731976158743 1107 10-11 0.14632262694947684 1107 12-13 0.19030460226993284 1107 14-15 0.3138946550595634 1107 16-17 0.1281657066132027 1107 18-19 0.11590391808829992 1107 20-21 0.14331596810686875 1107 22-23 0.2281476910243896 1107 24-25 0.31799311929243856 1107 26-27 0.18672813243843223 1107 28-29 0.20254187822263958 1107 30-31 0.2050326076462241 1107 32-33 0.22920491690162947 1107 34-35 0.10720759049623751 1107 36-37 0.20653352427587635 1107 38-39 0.2565898278417649 1107 40-41 0.4437251094073602 1107 42-43 3.484934011481755E-4 1107 44-45 0.17706900689881877 1107 46-47 0.2561116298320485 1107 48-49 0.28954737739464065 1107 50-51 0.3107776747812636 1107 52-53 0.33905904855874525 1107 54-55 0.42504336731768433 1107 56-57 0.45243910476400373 1107 58-59 0.3647843740412853 1107 60-61 0.49722915894385267 1107 62-63 0.5037156074044233 1107 64-65 0.260473971734271 1107 66-67 0.3763827646405531 1107 68-69 0.36961758299832326 1107 70-71 0.39808527243329905 1107 72-73 0.25479357689985704 1107 74-75 0.28433800146063604 1107 76-77 0.33652593521637897 1107 78-79 0.35266212906690697 1107 80-81 0.49531153507294334 1107 82-83 0.55083935054639 1107 84-85 0.581705326584327 1107 86-87 0.3081950311637591 1107 88-89 0.5413863313692993 1107 90-91 0.6139988256175215 1107 92-93 0.2723714545163993 1107 94-95 0.26269686928563374 1107 96-97 0.4829561986861286 1107 98-99 0.4615678580794089 1107 100-101 0.5226878967581605 1108 1 -0.15225759081974743 1108 2 -0.1743662187876751 1108 3 -0.16151598404888645 1108 4 -0.14458334032165254 1108 5 -0.20545452329756841 1108 6 -0.23004512825181678 1108 7 -0.174161904684631 1108 8 -0.1752714380890339 1108 9 -0.16190499447760942 1108 10-11 -0.2310236670854522 1108 12-13 -0.24733531965132727 1108 14-15 -0.33436880982350203 1108 16-17 -0.07258004253570505 1108 18-19 -0.1461790914587766 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-1.7692465889797475 2316 72-73 -1.7736116540784082 2316 74-75 -1.7727272159306722 2316 76-77 -1.966024449747394 2316 78-79 -1.9470151807157023 2316 80-81 -1.7801640084053183 2316 82-83 -1.955359698366653 2316 84-85 -2.2518261498649466 2316 86-87 -2.204684271372475 2316 88-89 -2.2152656160944666 2316 90-91 -2.3923700602520412 2316 92-93 -2.3231193742879483 2316 94-95 -2.1871446162216124 2316 96-97 -2.3773529045747424 2316 98-99 -2.4136019788771144 2316 100-101 -2.329313348712855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20099.0 3 4296.0 4 3368.0 5 2865.0 6 2785.0 7 2919.0 8 3425.0 9 4094.0 10 4748.0 11 5520.0 12 6831.0 13 9385.0 14 9484.0 15 8282.0 16 9569.0 17 11501.0 18 10856.0 19 8699.0 20 8811.0 21 9087.0 22 9628.0 23 10844.0 24 12169.0 25 14102.0 26 16321.0 27 19574.0 28 23163.0 29 27986.0 30 34203.0 31 41995.0 32 53080.0 33 69202.0 34 96232.0 35 143208.0 36 237785.0 37 384159.0 38 340639.0 39 60680.0 40 75.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.32554201378737 17.103182376158113 12.44970184430879 24.121573765745726 2 16.069229068629 18.528480331215384 39.83577045763194 25.56652014252368 3 20.782670561395673 22.535413457027538 27.379401308872414 29.302514672704376 4 13.056832021203842 14.247825670041589 34.95901114568398 37.73633116307059 5 14.695879310651733 36.71767739727561 32.99634975932069 15.590093532751967 6 35.57122973674419 33.62333886089381 16.19469951354405 14.610731888817943 7 31.65354439217992 30.697509370865795 18.764240041158313 18.88470619579597 8 28.007078958988778 34.88121440207546 17.99949353976086 19.11221309917491 9 27.19269785029904 16.056862131439843 18.614050149161166 38.13638986909995 10-11 26.829286498240005 25.333732193748553 25.81718776421034 22.019793543801104 12-13 27.097443429937073 22.10350999389611 26.862992122367157 23.936054453799656 14-15 23.562082674306733 23.905834011141415 24.619520700445385 27.912562614106466 16-17 24.01487928170569 27.07805305948411 25.069895583736155 23.837172075074044 18-19 23.874970463282164 26.063142396696136 26.6998147836046 23.362072356417098 20-21 24.465708355224063 25.199054894766608 26.911498301853747 23.423738448155582 22-23 24.02402988691345 25.02271033328778 26.9172861524963 24.03597362730247 24-25 23.96899460750814 25.222734607852708 27.04664878740718 23.76162199723197 26-27 23.936468307651317 25.359448676930285 26.941539634671525 23.762543380746873 28-29 23.711793402264327 25.75531698016774 26.94332861478561 23.589561002782318 30-31 23.901695309438118 25.36963315909838 26.79562254421314 23.933048987250366 32-33 23.734333947088786 25.327045139306602 27.08576603198055 23.852854881624058 34-35 24.147220955775687 25.476241462999038 26.786380403697102 23.590157177528173 36-37 24.124560416312754 25.318491774777407 26.8921530652535 23.66479474365634 38-39 23.940226223894108 25.59709027815231 26.813954367019843 23.648729130933734 40-41 24.062063339498742 25.568935099340806 26.80597879452567 23.56302276663478 42-43 23.831314604677136 25.534424916879413 26.61174040244584 24.022520075997615 44-45 23.94661055551597 25.53232367494958 26.688693024252746 23.832372745281706 46-47 24.195551570469238 25.813665864937306 26.44356722539615 23.547215339197294 48-49 24.30975521724567 25.786795527939127 26.225796316444093 23.67765293837111 50-51 23.836778504899414 25.983432014126933 26.376366238472816 23.803423242500838 52-53 24.2311670438956 25.943945078929566 26.120744994684173 23.704142882490665 54-55 24.01995330727626 25.790878562281822 26.222554033716495 23.966614096725422 56-57 23.956839928114622 25.954889907489402 26.266777821688496 23.82149234270747 58-59 24.252508081909763 25.86237085314448 26.132109188785968 23.75301187615979 60-61 24.03867815596278 25.837935849667254 26.42932326207474 23.694062732295226 62-63 24.008660313731575 25.927117102590852 26.394082926599914 23.67013965707765 64-65 23.79528360454838 26.079484741412934 26.24444088004302 23.880790773995667 66-67 23.89411082686079 26.1362882548216 26.083969531698397 23.885631386619213 68-69 23.699082115694907 25.899673459708666 26.375506622112482 24.025737802483945 70-71 23.917094781085492 25.749283158929163 26.197919956665043 24.1357021033203 72-73 23.922371456354423 26.0247102184435 26.139056493232832 23.913861831969246 74-75 23.77134878138848 26.399239521787297 25.884935838257018 23.94447585856721 76-77 24.037038372503915 26.13100449277766 25.879178175052463 23.952778959665963 78-79 23.97732612460016 26.17392631250106 25.958160463446582 23.89058709945219 80-81 24.021508198991228 26.12334617498201 25.970457957903815 23.88468766812295 82-83 24.058418686871892 26.213291439760777 25.999563832984958 23.728726040382373 84-85 24.047956696929276 26.28834366446813 25.772585029931825 23.89111460867077 86-87 23.95571090931277 26.189839483490108 26.010753795546336 23.843695811650782 88-89 23.94122706264857 26.220182993909148 25.75443224888972 24.084157694552566 90-91 23.90986222441926 26.29484026222465 25.845957879797638 23.949339633558452 92-93 23.98221054186692 26.28293467772838 25.888059029653327 23.846795750751372 94-95 24.01057745618352 26.151338278402754 26.00399005601425 23.834094209399478 96-97 23.973285405950392 26.014832725204673 26.086931443923923 23.92495042492101 98-99 23.823512618311995 26.16558690420095 25.75485806597846 24.256042411508595 100-101 23.97446701008273 26.196087456315965 25.580603279467653 24.248842254133653 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 72.0 1 62.5 2 124.5 3 239.5 4 219.5 5 184.0 6 336.5 7 705.0 8 1174.0 9 1365.0 10 1230.5 11 1143.5 12 1232.0 13 1410.5 14 1603.0 15 1740.5 16 1833.5 17 1967.5 18 2101.5 19 2231.5 20 2361.5 21 2530.0 22 2830.5 23 3282.5 24 3889.5 25 4755.5 26 5915.0 27 7370.5 28 8924.0 29 10760.0 30 12800.5 31 15154.0 32 17934.5 33 20222.0 34 22195.5 35 24425.0 36 27552.5 37 31003.5 38 33912.0 39 36481.0 40 39564.5 41 42585.5 42 45152.5 43 47975.5 44 51419.0 45 55044.0 46 58103.0 47 62928.5 48 69655.5 49 71241.0 50 68185.0 51 67521.5 52 69082.0 53 71050.0 54 72823.5 55 71372.5 56 66775.0 57 62143.0 58 59234.5 59 54741.0 60 47266.0 61 39445.5 62 32179.0 63 25615.5 64 19372.5 65 14472.0 66 10449.5 67 7948.0 68 6106.0 69 4538.0 70 3464.0 71 2338.5 72 1713.0 73 1428.5 74 1118.0 75 789.5 76 542.0 77 315.5 78 182.0 79 115.0 80 69.5 81 38.5 82 29.5 83 21.0 84 22.5 85 24.5 86 21.0 87 20.5 88 19.0 89 16.0 90 17.0 91 15.5 92 15.0 93 15.0 94 12.0 95 12.0 96 17.5 97 15.0 98 9.5 99 12.5 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0459329528170967 2 0.01171290296835966 3 0.009760752473633049 4 0.005626786720094346 5 0.008038266742991924 6 0.005339705764987492 7 0.0057990352931584594 8 0.009760752473633049 9 0.015157874429641912 10-11 0.002325355736365521 12-13 0.008956925799333857 14-15 0.008526304366673575 16-17 0.01845930541337074 18-19 0.011052616771613894 20-21 0.0036746362253677364 22-23 0.00981816866465442 24-25 0.020612412576672148 26-27 0.0064880295854149094 28-29 0.018028683980710457 30-31 0.014153091086767922 32-33 0.011741611063870346 34-35 0.25980826437170323 36-37 0.4632625372559309 38-39 0.4611955543791616 40-41 0.4790232816912972 42-43 0.47043956113360225 44-45 0.4847361926979237 46-47 0.49036297941801804 48-49 0.4962194309021979 50-51 0.5057792267072561 52-53 0.49587493375606956 54-55 0.4835878688774963 56-57 0.4796548597925323 58-59 0.47784624977535917 60-61 0.47583668308961113 62-63 0.4733677868756922 64-65 0.4869467160522464 66-67 0.46300416439633474 68-69 0.4879514993951204 70-71 0.4705543935156451 72-73 0.47870749264067974 74-75 0.4908510170416997 76-77 0.4783629954945515 78-79 0.47704242310105993 80-81 0.4809467240905132 82-83 0.48160701028725894 84-85 0.4821237560064513 86-87 0.48347303649545353 88-89 0.4972529223405825 90-91 0.5186117454005326 92-93 0.5078462095840255 94-95 0.4799993569386605 96-97 0.45559747575457793 98-99 0.44784628996669285 100-101 0.45427690336108645 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1741669.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.23687165155055 #Duplication Level Percentage of deduplicated Percentage of total 1 78.36916605042845 45.63975064717854 2 12.192546428556724 14.201115229308577 3 3.936696626650274 6.8778268853197195 4 1.7721216025599889 4.128112732769045 5 0.9415142253177444 2.7415421548969263 6 0.5917115811453658 2.067565884353922 7 0.41422048636360265 1.6886033709859982 8 0.2928013125578725 1.3641465967070685 9 0.22106478526135878 1.1586709373349 >10 1.151192808734944 12.182850988679451 >50 0.07973054394426934 3.1791106227707764 >100 0.03525862022651591 3.5050363132928566 >500 0.0014811961896411309 0.5858794983717994 >1k 4.937320632137103E-4 0.6797881380303338 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3745 0.21502363537503397 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2573 0.14773185949798728 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2467 0.14164574324972196 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1894 0.10874626579447644 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1483238204274176E-4 2 0.0 0.0 0.0 0.0 1.1483238204274176E-4 3 0.0 0.0 0.0 0.0 1.1483238204274176E-4 4 0.0 0.0 0.0 0.0 1.1483238204274176E-4 5 0.0 0.0 0.0 0.0 1.1483238204274176E-4 6 0.0 0.0 0.0 0.0 1.1483238204274176E-4 7 1.1483238204274176E-4 0.0 0.0 0.0 1.7224857306411264E-4 8 1.1483238204274176E-4 0.0 0.0 0.0 1.7224857306411264E-4 9 1.1483238204274176E-4 0.0 0.0 0.0 1.7224857306411264E-4 10-11 1.7224857306411264E-4 0.0 0.0 0.0 1.7224857306411264E-4 12-13 1.7224857306411264E-4 0.0 0.0 2.870809551068544E-5 2.2966476408548353E-4 14-15 1.7224857306411264E-4 0.0 0.0 5.741619102137088E-5 2.5837285959616897E-4 16-17 1.7224857306411264E-4 0.0 0.0 5.741619102137088E-5 2.870809551068544E-4 18-19 2.0095666857479808E-4 0.0 0.0 1.1483238204274176E-4 3.444971461282253E-4 20-21 2.2966476408548353E-4 0.0 0.0 1.435404775534272E-4 4.5932952817096705E-4 22-23 2.870809551068544E-4 0.0 0.0 4.5932952817096705E-4 5.167457191923379E-4 24-25 4.5932952817096705E-4 0.0 0.0 5.454538147030234E-4 5.167457191923379E-4 26-27 4.5932952817096705E-4 0.0 0.0 6.889942922564507E-4 5.454538147030234E-4 28-29 4.880376236816525E-4 0.0 0.0 0.0010047833428739905 5.741619102137088E-4 30-31 5.167457191923379E-4 0.0 0.0 0.002038274781258666 5.741619102137088E-4 32-33 5.454538147030234E-4 0.0 0.0 0.00358851193883568 5.741619102137088E-4 34-35 6.028700057243942E-4 0.0 0.0 0.006315781012350797 5.741619102137088E-4 36-37 6.889942922564506E-4 0.0 0.0 0.012229648687551997 5.741619102137088E-4 38-39 6.889942922564506E-4 0.0 0.0 0.0224784387848667 6.028700057243942E-4 40-41 6.889942922564506E-4 0.0 0.0 0.03301430983728826 6.315781012350797E-4 42-43 6.889942922564506E-4 0.0 0.0 0.04418175899094489 6.315781012350797E-4 44-45 7.464104832778215E-4 0.0 0.0 0.05641140767849689 6.315781012350797E-4 46-47 7.464104832778215E-4 0.0 0.0 0.07073674733832892 6.315781012350797E-4 48-49 7.464104832778215E-4 0.0 0.0 0.0885357665549539 6.315781012350797E-4 50-51 7.464104832778215E-4 0.0 0.0 0.10768406626058108 6.315781012350797E-4 52-53 7.464104832778215E-4 0.0 0.0 0.12786585740459294 6.315781012350797E-4 54-55 7.464104832778215E-4 0.0 0.0 0.15037300428497036 6.315781012350797E-4 56-57 7.464104832778215E-4 0.0 0.0 0.17721507358746122 6.315781012350797E-4 58-59 7.464104832778215E-4 0.0 0.0 0.20704278482306337 6.315781012350797E-4 60-61 7.464104832778215E-4 0.0 0.0 0.24249728277875993 6.315781012350797E-4 62-63 7.464104832778215E-4 0.0 0.0 0.2777795321613923 6.315781012350797E-4 64-65 7.464104832778215E-4 0.0 0.0 0.31363594345423845 6.315781012350797E-4 66-67 7.464104832778215E-4 0.0 0.0 0.3576167457766085 6.315781012350797E-4 68-69 7.464104832778215E-4 0.0 0.0 0.40825782625745766 6.315781012350797E-4 70-71 7.464104832778215E-4 0.0 0.0 0.4689467401670466 6.315781012350797E-4 72-73 8.038266742991923E-4 0.0 0.0 0.5299514431272532 6.889942922564507E-4 74-75 8.612428653205632E-4 0.0 0.0 0.5910709784695025 7.464104832778215E-4 76-77 8.612428653205632E-4 0.0 0.0 0.6594823700714659 8.038266742991923E-4 78-79 9.18659056341934E-4 0.0 0.0 0.7416449394230477 9.760752473633049E-4 80-81 0.0011483238204274175 0.0 0.0 0.8356639522205425 9.760752473633049E-4 82-83 0.0011483238204274175 0.0 0.0 0.934190136013215 0.0010909076294060467 84-85 0.0011483238204274175 0.0 0.0 1.0333478979071224 0.0010909076294060467 86-87 0.001177031915938103 0.0 0.0 1.1394243108191051 0.001177031915938103 88-89 0.0012057400114487884 0.0 0.0 1.2606873062562405 0.0012631562024701594 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1705 0.0 30.26106 2 GTACCTG 1745 0.0 30.120296 1 GTACATG 6445 0.0 26.816471 1 TACATGG 6320 0.0 26.588467 2 ACATGGG 6500 0.0 24.39501 3 GTATCAA 7210 0.0 23.31443 1 TACCGGG 185 2.6300404E-7 23.027819 2 CATGGGG 4380 0.0 21.937092 4 CCGTATC 130 3.014168E-4 21.845022 9 CAACGCA 8330 0.0 20.171604 5 TCAACGC 8320 0.0 20.13896 4 ATCAACG 8330 0.0 20.11536 3 TATCAAC 8410 0.0 19.868301 2 AACGCAG 8580 0.0 19.803947 6 GAGTACA 5155 0.0 19.565126 1 AGTACAT 4695 0.0 19.357428 2 GGTATCA 5585 0.0 19.24573 1 ACCTGGG 2720 0.0 18.794224 3 GTACTTT 3975 0.0 18.101452 14-15 AGAGTAC 6775 0.0 17.990004 10-11 >>END_MODULE