FastQCFastQC Report
Thu 26 May 2016
SRR936295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741669
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39080.2243824745115174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29270.16805719111955258No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26500.15215290620663285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19750.11339697726720749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG15700.028.4056132
TACATGG64300.028.2594532
GTACATG66900.028.1615371
ACATGGG64650.026.8535213
GTACCTG16850.026.2585261
GTATCAA71550.024.3365351
GGTATCA53250.022.3361171
CATGGGG42500.021.7637834
GTATAGG3950.021.680171
ATAACGC1100.00290130921.5608563
GTAGGAC4000.021.3452473
CAACGCA81350.021.0499385
AGTACAT45100.020.9340132
ATCAACG81550.020.8813783
TCAACGC81650.020.8558034
GAGTACA51800.020.481531
GTGTAGG8850.020.4280831
AACGCAG83700.020.3455836
TATCAAC84850.020.1851222
GTCTACG1707.8597885E-519.5900881
Produced by FastQC (version 0.11.5)