##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936294_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 765736 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.969057220765382 33.0 31.0 34.0 30.0 34.0 2 32.06998234378428 34.0 31.0 34.0 30.0 34.0 3 32.121399542401036 34.0 31.0 34.0 30.0 34.0 4 35.631106543247284 37.0 35.0 37.0 35.0 37.0 5 35.665660227545786 37.0 35.0 37.0 35.0 37.0 6 35.78897296196078 37.0 35.0 37.0 35.0 37.0 7 35.7460129861989 37.0 35.0 37.0 35.0 37.0 8 35.75637817733527 37.0 35.0 37.0 35.0 37.0 9 37.45606057440162 39.0 38.0 39.0 35.0 39.0 10-11 37.39919894062706 39.0 37.5 39.0 35.0 39.0 12-13 37.36822808383046 39.0 37.0 39.0 35.0 39.0 14-15 38.59380909347347 40.0 38.0 41.0 34.5 41.0 16-17 38.583696600394916 40.0 38.0 41.0 34.5 41.0 18-19 38.64197908939896 40.0 38.5 41.0 35.0 41.0 20-21 38.59164973306727 40.0 38.0 41.0 34.5 41.0 22-23 38.43273465006216 40.0 38.0 41.0 34.0 41.0 24-25 38.58354380622042 40.0 38.0 41.0 35.0 41.0 26-27 38.509242741623744 40.0 38.0 41.0 34.5 41.0 28-29 38.401737674603254 40.0 38.0 41.0 34.0 41.0 30-31 38.24114054974561 40.0 38.0 41.0 34.0 41.0 32-33 38.01562744862459 40.0 38.0 41.0 34.0 41.0 34-35 37.747657808957655 40.0 37.5 41.0 33.0 41.0 36-37 37.550100818036505 40.0 37.0 41.0 33.0 41.0 38-39 37.39045571841993 40.0 37.0 41.0 33.0 41.0 40-41 37.29029326034038 40.0 37.0 41.0 33.0 41.0 42-43 37.09515420458226 40.0 37.0 41.0 32.0 41.0 44-45 36.87817864120271 40.0 37.0 41.0 31.5 41.0 46-47 36.552833483080335 40.0 37.0 41.0 30.5 41.0 48-49 36.337510578058236 40.0 37.0 41.0 30.0 41.0 50-51 35.98941671803337 39.5 36.5 40.5 29.5 41.0 52-53 36.0697753533855 39.5 37.0 40.5 30.0 41.0 54-55 36.217714721522825 40.0 37.0 41.0 29.5 41.0 56-57 36.11518852450453 40.0 36.5 41.0 29.0 41.0 58-59 35.966306925624494 40.0 36.0 41.0 28.0 41.0 60-61 35.76109455478128 40.0 36.0 41.0 28.0 41.0 62-63 35.51970195994442 39.0 35.5 41.0 28.0 41.0 64-65 35.258501624580795 39.0 35.0 41.0 27.5 41.0 66-67 34.92929025147048 39.0 35.0 40.0 26.0 41.0 68-69 34.55057683065704 38.0 35.0 40.0 26.0 41.0 70-71 34.130035025126155 37.0 34.5 39.5 25.5 41.0 72-73 33.698898837197156 37.0 34.0 39.0 24.5 41.0 74-75 33.25643433245923 36.0 34.0 39.0 23.5 40.5 76-77 32.842526014187655 36.0 34.0 38.5 22.0 40.0 78-79 32.39982709445553 35.0 33.5 37.0 20.5 39.0 80-81 31.944757723288443 35.0 33.0 37.0 19.0 39.0 82-83 31.530317890238933 35.0 33.0 36.0 15.5 38.5 84-85 31.124953639374404 35.0 32.0 36.0 9.5 37.0 86-87 30.825460733203087 35.0 32.0 35.5 4.5 37.0 88-89 30.455749762320174 35.0 32.0 35.0 2.0 36.5 90-91 30.10176940877796 35.0 31.5 35.0 2.0 36.0 92-93 29.810302114566902 34.5 31.0 35.0 2.0 36.0 94-95 29.456586212480545 34.0 31.0 35.0 2.0 36.0 96-97 29.087415244940814 34.0 31.0 35.0 2.0 35.0 98-99 28.720602792607373 34.0 31.0 35.0 2.0 35.0 100-101 25.559382737653706 30.5 24.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19381564101818682 1101 2 0.0661868604733229 1101 3 0.14899079659763714 1101 4 -0.08218966898480318 1101 5 0.17159658630352936 1101 6 0.24987594676035485 1101 7 0.3298337820618755 1101 8 0.13509624405464393 1101 9 0.20587855018833778 1101 10-11 0.2979629900153 1101 12-13 0.32073315440841554 1101 14-15 0.5186495345213373 1101 16-17 0.34065701298092677 1101 18-19 0.5754610300617671 1101 20-21 0.5385010711727674 1101 22-23 0.6872880908876766 1101 24-25 0.5212618706091163 1101 26-27 0.43404024307533007 1101 28-29 0.3895490390591405 1101 30-31 0.6290606255296822 1101 32-33 0.7146116505685711 1101 34-35 0.3759587347810438 1101 36-37 0.6414165712705753 1101 38-39 0.5606204188349224 1101 40-41 0.21306829293293816 1101 42-43 0.42954284765198025 1101 44-45 0.44423869698690766 1101 46-47 0.543546713950164 1101 48-49 0.3491563677152101 1101 50-51 0.4014019044940369 1101 52-53 0.3084541539075758 1101 54-55 0.4094942616966719 1101 56-57 0.37631495304714235 1101 58-59 0.45867763874929324 1101 60-61 0.3508763734434339 1101 62-63 0.2159509160578139 1101 64-65 0.1989966980471607 1101 66-67 0.3597406351336616 1101 68-69 0.4777463295346962 1101 70-71 0.4275568929810518 1101 72-73 0.3900378022069759 1101 74-75 0.2562127239839427 1101 76-77 0.2032969003185343 1101 78-79 0.40470165284502 1101 80-81 0.48420202280372493 1101 82-83 0.6182164157001395 1101 84-85 0.6429923223962106 1101 86-87 0.5770875932043786 1101 88-89 0.364759813564433 1101 90-91 0.4101041269508947 1101 92-93 0.2412444696658298 1101 94-95 0.5850686402313485 1101 96-97 0.5301149021417793 1101 98-99 0.5862217392165583 1101 100-101 1.0200986434126342 1102 1 0.2977322953992392 1102 2 0.4140024779483795 1102 3 0.2905210929266957 1102 4 0.19662010053703938 1102 5 0.17981812053125878 1102 6 0.1880891267691993 1102 7 0.11389196673925284 1102 8 0.12983308615990552 1102 9 0.14904037632229716 1102 10-11 0.24946920653699323 1102 12-13 0.3049829946954077 1102 14-15 0.4239765776192783 1102 16-17 0.3443156694259599 1102 18-19 0.31150211316167287 1102 20-21 0.4538114058329441 1102 22-23 0.3859981012075977 1102 24-25 0.44057240183915525 1102 26-27 0.3323177997120226 1102 28-29 0.2588177991947731 1102 30-31 0.24390410678183372 1102 32-33 0.17140559621700646 1102 34-35 0.062422443071376676 1102 36-37 0.0815094505275411 1102 38-39 0.04149908609129227 1102 40-41 -0.2108960296381852 1102 42-43 -0.4182285416054725 1102 44-45 -0.3358856334470204 1102 46-47 -0.33183045446106263 1102 48-49 -0.6440226582811519 1102 50-51 -0.7980083859379263 1102 52-53 -0.8211787518863147 1102 54-55 -0.7039240880993844 1102 56-57 -0.5454649869990504 1102 58-59 -0.5779506967954475 1102 60-61 -0.5990793983389651 1102 62-63 -0.5286704412570415 1102 64-65 -0.5006224478568129 1102 66-67 -0.5199217561386433 1102 68-69 -0.6069851068995291 1102 70-71 -0.4402719929592891 1102 72-73 -0.4121908085355699 1102 74-75 -0.5762360449973372 1102 76-77 -0.689417729393 1102 78-79 -0.7696287810829361 1102 80-81 -0.6971631548462831 1102 82-83 -0.6049911129253474 1102 84-85 -0.6500687616865441 1102 86-87 -0.6675558782192788 1102 88-89 -0.6496094429100836 1102 90-91 -0.5456725696926732 1102 92-93 -0.6616696784495559 1102 94-95 -0.7239883158844336 1102 96-97 -0.5691086469269706 1102 98-99 -0.5670192062844457 1102 100-101 -0.35066650475189576 1103 1 -0.05070095349840642 1103 2 -0.06462122033475737 1103 3 -0.2639962163893763 1103 4 -0.3347149215100558 1103 5 -0.5172923025853606 1103 6 -0.4193881225037117 1103 7 -0.19108973886164904 1103 8 -0.258843149884747 1103 9 -0.2760839317741457 1103 10-11 -0.23421594216363673 1103 12-13 -0.2178310591557988 1103 14-15 -0.02611958524777691 1103 16-17 -0.04098079365688534 1103 18-19 -0.12089539129465265 1103 20-21 -0.060200227528532935 1103 22-23 -0.0043785757789933655 1103 24-25 -0.020476946129697637 1103 26-27 0.11127689531198115 1103 28-29 -0.026035376525278764 1103 30-31 -0.14795957649052127 1103 32-33 -0.10180249084557147 1103 34-35 -0.06848570966339906 1103 36-37 -0.02903797418397147 1103 38-39 0.10791479112928926 1103 40-41 0.19456179942645235 1103 42-43 0.2844851275942588 1103 44-45 0.22796885571706582 1103 46-47 0.16439808480154028 1103 48-49 0.4351592537180977 1103 50-51 0.556272034364163 1103 52-53 0.5006619461153718 1103 54-55 0.5902662674686852 1103 56-57 0.5317983585305512 1103 58-59 0.5178767729484335 1103 60-61 0.49622269378975403 1103 62-63 0.45830300840990645 1103 64-65 0.5505479245983835 1103 66-67 0.5356064359994548 1103 68-69 0.32792670471507535 1103 70-71 0.4463519867761505 1103 72-73 0.3434288555980274 1103 74-75 0.4798057975770149 1103 76-77 0.3226331196547534 1103 78-79 0.4234967466401187 1103 80-81 0.2873405509422504 1103 82-83 0.5476897201734445 1103 84-85 0.4239086283125175 1103 86-87 0.5509331920499783 1103 88-89 0.544449568254187 1103 90-91 0.8028169696637413 1103 92-93 0.682297860719224 1103 94-95 0.7947728249355315 1103 96-97 1.0720701690970458 1103 98-99 0.9002549282497476 1103 100-101 1.2826527559667475 1104 1 0.06976351652938106 1104 2 0.02072349317589328 1104 3 0.06123866517738463 1104 4 -1.906393875188428E-4 1104 5 0.19668149654128086 1104 6 0.09666322062186339 1104 7 0.10081180937177692 1104 8 0.0822578161070453 1104 9 0.15030220028884145 1104 10-11 0.22030304685316793 1104 12-13 0.19437285774245083 1104 14-15 0.05985699275937151 1104 16-17 0.3072890224076943 1104 18-19 0.24353824639225508 1104 20-21 0.23665037456224525 1104 22-23 0.23590526701577375 1104 24-25 0.20715637397086795 1104 26-27 0.2423441177547474 1104 28-29 0.24669189620200171 1104 30-31 0.17639201805757665 1104 32-33 0.35007986987958617 1104 34-35 0.2358083223598868 1104 36-37 0.19469168044087581 1104 38-39 0.06033969519175031 1104 40-41 0.16596910391235298 1104 42-43 0.3344156555815587 1104 44-45 0.2558072836646659 1104 46-47 0.343979929448885 1104 48-49 0.09908012178743775 1104 50-51 0.14915611534868134 1104 52-53 0.15995134667114286 1104 54-55 0.15569236247993246 1104 56-57 0.34549426960637675 1104 58-59 0.22066557802980924 1104 60-61 0.12345903096058919 1104 62-63 0.4163286799726933 1104 64-65 0.18872775786555707 1104 66-67 0.2797343968304773 1104 68-69 0.13040453983482792 1104 70-71 0.12554677306700057 1104 72-73 0.16340371334580794 1104 74-75 0.2682734434682388 1104 76-77 0.112682080882756 1104 78-79 0.10451103802558492 1104 80-81 0.06787846281204324 1104 82-83 0.14282998747528097 1104 84-85 -0.007489413238364051 1104 86-87 0.13051172850624582 1104 88-89 0.34318914745228213 1104 90-91 0.3598303347557099 1104 92-93 0.31101295930331574 1104 94-95 0.054674240841315225 1104 96-97 0.3532035553614392 1104 98-99 0.6315603404503598 1104 100-101 0.7392294398360058 1105 1 0.0331019246373252 1105 2 0.08099636150950573 1105 3 0.13443863426929425 1105 4 -0.16715698924624434 1105 5 -0.04120430641128081 1105 6 -0.03538742688178331 1105 7 0.008062471911799207 1105 8 0.012714608473586964 1105 9 -0.08663129927990809 1105 10-11 0.0484195954216915 1105 12-13 -0.05343277212479336 1105 14-15 -7.761219200972391E-4 1105 16-17 0.1356582655144507 1105 18-19 0.11889092234388698 1105 20-21 0.17228531657823964 1105 22-23 0.06686723962034335 1105 24-25 0.041625788534013 1105 26-27 0.05569996387422549 1105 28-29 0.07141690815799961 1105 30-31 0.05527615239083161 1105 32-33 -0.11232920707529814 1105 34-35 -0.3780744231975959 1105 36-37 -0.1841214488155103 1105 38-39 0.06809804403133768 1105 40-41 -0.08190335426252204 1105 42-43 0.146522647702092 1105 44-45 -0.10506557756844614 1105 46-47 0.04304683920351948 1105 48-49 0.11164071104603579 1105 50-51 -0.0025504081383331823 1105 52-53 0.08501697415763232 1105 54-55 0.26947695396057014 1105 56-57 0.11380783637933689 1105 58-59 0.3374574433540687 1105 60-61 0.3371133072413457 1105 62-63 0.04470794732559824 1105 64-65 0.06884821589006407 1105 66-67 0.07862087015449504 1105 68-69 0.14692261793624084 1105 70-71 0.07651045814695578 1105 72-73 0.1708341858010627 1105 74-75 0.0457529303103712 1105 76-77 0.11865068410846646 1105 78-79 0.15085751356328814 1105 80-81 0.1786202779106425 1105 82-83 0.09271711028691243 1105 84-85 -0.07232957872195911 1105 86-87 -9.3955261329981E-4 1105 88-89 -0.009157883595733551 1105 90-91 0.0861329124488357 1105 92-93 0.13095661468643982 1105 94-95 0.3886372221356531 1105 96-97 0.4218299621495234 1105 98-99 0.4935410897209884 1105 100-101 0.6936587991822556 1106 1 -0.022959510289336293 1106 2 -0.11998420267615728 1106 3 0.08198154685457837 1106 4 0.11765045891287684 1106 5 -0.08683666709374904 1106 6 -0.0979143923968877 1106 7 -0.08324147202057475 1106 8 -0.11022749695254674 1106 9 -0.048135381523437104 1106 10-11 -0.039469920798907765 1106 12-13 -0.085593213069032 1106 14-15 -0.08749412198202577 1106 16-17 0.03563246119378505 1106 18-19 0.09302983216294791 1106 20-21 -0.014702080591533218 1106 22-23 0.04532166402916715 1106 24-25 0.054206943122537155 1106 26-27 -0.009559163113998181 1106 28-29 0.037579186369860906 1106 30-31 -0.18193058150469454 1106 32-33 -0.15501264999098652 1106 34-35 -0.33958882716940053 1106 36-37 -0.15066975966468021 1106 38-39 -0.21473072881838817 1106 40-41 -0.2891584970636316 1106 42-43 -0.28654713750274396 1106 44-45 -0.27113186370739584 1106 46-47 -0.09112840309905579 1106 48-49 -0.17472738242753394 1106 50-51 -0.1931338954374482 1106 52-53 -0.28347801464330047 1106 54-55 -0.2922985897364683 1106 56-57 -0.22643142128196558 1106 58-59 -0.4285097541934917 1106 60-61 -0.10282352378164461 1106 62-63 -0.4126148027956802 1106 64-65 -0.46255977106326185 1106 66-67 -0.3580222973774738 1106 68-69 -0.3582134740510057 1106 70-71 -0.29839948477388845 1106 72-73 -0.10574271623541875 1106 74-75 -0.2842120790307874 1106 76-77 -0.47778109442852923 1106 78-79 -0.5475137177156739 1106 80-81 -0.4583810535923014 1106 82-83 -0.4442821568703792 1106 84-85 -0.317496429459446 1106 86-87 -0.23236198998621305 1106 88-89 -0.14885215400436635 1106 90-91 0.11117755392360706 1106 92-93 -0.028842318046855553 1106 94-95 -0.09148725790728562 1106 96-97 -0.13044236632915585 1106 98-99 -0.09485625553049815 1106 100-101 0.08669679346401793 1107 1 -0.03702002658218362 1107 2 0.19527182779358299 1107 3 0.2103353344127612 1107 4 0.28725477545963685 1107 5 0.14692338368914903 1107 6 0.22655661902926028 1107 7 0.16706534135225581 1107 8 0.29686094993699896 1107 9 0.41986080975883056 1107 10-11 0.3370853056082055 1107 12-13 0.2708498677604254 1107 14-15 0.3190230172477655 1107 16-17 0.262272325772706 1107 18-19 0.34311976022049606 1107 20-21 0.3371705389598816 1107 22-23 0.22674887520140175 1107 24-25 0.32114515725711357 1107 26-27 0.2861877687522707 1107 28-29 0.2057605236529767 1107 30-31 0.400792651673477 1107 32-33 0.3606083825947124 1107 34-35 0.2253868393562044 1107 36-37 0.08994598303527823 1107 38-39 0.07830249166404712 1107 40-41 0.2341117102998922 1107 42-43 -0.06319758185315294 1107 44-45 0.45149826748176736 1107 46-47 0.4402670687587573 1107 48-49 0.37602378134732817 1107 50-51 0.2707996635976784 1107 52-53 0.15393734718488616 1107 54-55 0.2519195651513897 1107 56-57 0.2668028148585364 1107 58-59 0.27649976760083916 1107 60-61 0.11516208772914638 1107 62-63 0.1908342027058012 1107 64-65 0.13385897629179055 1107 66-67 0.30109217574990055 1107 68-69 0.3993616423264825 1107 70-71 0.5768332701986054 1107 72-73 0.39601352582975835 1107 74-75 0.40712308812960174 1107 76-77 0.6213407845388872 1107 78-79 0.3628015594743417 1107 80-81 0.40423003400820434 1107 82-83 0.7006977415638147 1107 84-85 0.6863863466725881 1107 86-87 0.5286515521212785 1107 88-89 0.7566599069351163 1107 90-91 1.0051788234961805 1107 92-93 0.6052033865659254 1107 94-95 0.35542344882145116 1107 96-97 0.7746994026086327 1107 98-99 0.7660303293192676 1107 100-101 0.4681028450933056 1108 1 -0.07331539579369917 1108 2 -0.23517247758814364 1108 3 -0.07688306358473085 1108 4 -0.07923458624248525 1108 5 -0.12810790542224026 1108 6 -0.11843712314846044 1108 7 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2316 82-83 -1.2602536625734793 2316 84-85 -1.2476740947927993 2316 86-87 -1.2211317600247966 2316 88-89 -1.604396951957753 2316 90-91 -1.6497876621157452 2316 92-93 -1.7721166180567494 2316 94-95 -1.585394764514966 2316 96-97 -1.8369150663616658 2316 98-99 -1.8090101190801313 2316 100-101 -2.0476532510583176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4299.0 3 654.0 4 537.0 5 466.0 6 491.0 7 602.0 8 643.0 9 877.0 10 1165.0 11 1579.0 12 2533.0 13 4127.0 14 4377.0 15 4246.0 16 5488.0 17 7845.0 18 6640.0 19 3447.0 20 3049.0 21 3050.0 22 3006.0 23 3478.0 24 3802.0 25 4256.0 26 4837.0 27 5957.0 28 7193.0 29 8642.0 30 10795.0 31 13547.0 32 17089.0 33 22636.0 34 31194.0 35 50695.0 36 75686.0 37 152091.0 38 240008.0 39 54690.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.99775144666513 23.09359652251624 14.661456189793057 21.247195841025583 2 13.643244401081972 24.115412093689063 41.339848152980714 20.901495352248254 3 16.637236335140077 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18.628369699300805 30.775645500548215 32.272513258074184 18.323471542076796 24-25 18.887201742441313 31.54863716663891 32.21021627040494 17.35394482051483 26-27 17.75394993322907 31.280793806792023 33.60292028954749 17.362335970431413 28-29 17.64594091374258 30.800884785259324 34.02981546075382 17.523358840244278 30-31 18.133580799515165 31.015449439119465 32.988800675002764 17.862169086362606 32-33 17.40705403864577 32.26717551768342 33.09360128989031 17.232169153780497 34-35 18.274513501356324 31.101800508220112 33.24337012396459 17.38031586645898 36-37 18.61039757759472 30.2489001742006 32.56849101603892 18.572211232165763 38-39 17.261983652184412 32.05027432259182 33.13549213381116 17.552249891412607 40-41 17.16054575963975 31.593999557690832 33.95713801030436 17.288316672365063 42-43 17.526017401803173 31.185054921980605 32.13998871376265 19.148938962453578 44-45 17.58974200792923 31.1764099784148 32.28093709576966 18.952910917886317 46-47 18.466885004889637 30.958631688795833 32.61257654417411 17.961906762140416 48-49 18.053539958058067 31.552212453274787 32.095816636966 18.298430951701143 50-51 17.51942208028778 31.212325102813125 32.30055238464089 18.967700432258212 52-53 17.84346479122841 31.965698645601897 31.844731565319268 18.34610499785042 54-55 18.401749378667372 30.969173895038594 31.7315081762583 18.897568550035736 56-57 18.773079409245874 31.41877458877882 30.94148567903584 18.866660322939467 58-59 18.555940616475166 30.51342744102629 31.9341644261896 18.996467516308947 60-61 18.797534224641744 32.03631204631269 31.11781466262266 18.048339066422905 62-63 17.98214358364601 32.01949690734638 32.11215183859669 17.886207670410922 64-65 18.024669157121817 31.37356564747021 31.82713738544213 18.774627809965846 66-67 18.292846157480067 31.728543458976553 30.63314576958003 19.34546461396335 68-69 17.855490369678087 31.43671526183043 31.590684902377603 19.11710946611388 70-71 19.126770969970856 30.834809209995324 31.964437223457683 18.07398259657614 72-73 18.807687739241082 30.71899799780684 31.654207919655025 18.819106343297047 74-75 18.029491276465457 32.33788625505944 30.639844004838423 18.992778463636682 76-77 17.911254843078993 31.04610506200191 32.00008662417707 19.04255347074203 78-79 17.693056806041533 30.913172670311816 31.8920078852802 19.501762638366454 80-81 18.641513990552653 31.82947525091645 30.49256667808538 19.036444080445513 82-83 19.14352558234157 30.891879210266403 31.209373902828773 18.755221304563246 84-85 17.94501597335062 31.145343052970624 31.192593720386302 19.717047253292453 86-87 18.434430274742873 31.651279210790207 31.080976431860304 18.83331408260662 88-89 18.619709194828175 30.974761135574962 31.177247018994294 19.22828265060257 90-91 18.550702373238323 30.100453455340197 31.768200090205244 19.580644081216235 92-93 19.608032349967832 31.323801646382293 30.958289022805154 18.10987698084472 94-95 18.800871763943857 29.783036828154746 31.915393484870457 19.500697923030934 96-97 18.95677447079316 30.09844411713571 31.454945660553136 19.489835751517994 98-99 18.744005657478514 30.923591239305303 30.734199536328177 19.598203566888007 100-101 18.56538617228776 30.240469115861202 31.238481881659514 19.955662830191525 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1846.0 1 1088.5 2 630.5 3 908.0 4 640.5 5 659.5 6 1465.0 7 2411.0 8 2892.5 9 2711.5 10 2013.0 11 1522.0 12 1560.5 13 1627.5 14 1561.0 15 1468.0 16 1464.5 17 1438.5 18 1348.0 19 1390.5 20 1603.0 21 1841.0 22 2175.0 23 2567.0 24 2802.0 25 3565.5 26 5841.0 27 8406.0 28 8623.0 29 9608.0 30 14170.5 31 18447.5 32 24660.0 33 32969.0 34 39816.0 35 45916.0 36 53464.0 37 57447.0 38 52540.5 39 47155.5 40 41379.5 41 34466.0 42 27384.0 43 25902.0 44 27640.5 45 24777.5 46 19382.5 47 18052.5 48 23945.0 49 21134.5 50 12553.5 51 7438.0 52 4150.5 53 2970.5 54 2343.0 55 1851.0 56 1330.5 57 1040.0 58 883.0 59 714.0 60 592.0 61 503.5 62 417.5 63 313.5 64 234.0 65 200.5 66 137.5 67 85.5 68 60.0 69 54.5 70 61.5 71 63.5 72 60.0 73 57.0 74 40.5 75 31.5 76 26.0 77 20.5 78 10.5 79 5.0 80 5.5 81 4.0 82 4.0 83 4.5 84 4.5 85 5.0 86 4.5 87 2.5 88 3.0 89 3.5 90 2.5 91 3.5 92 5.5 93 6.0 94 5.5 95 4.5 96 3.5 97 2.5 98 2.0 99 3.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04636062559419957 2 0.012667551218696784 3 0.01123102479183426 4 0.005746105707450087 5 0.00940271843037287 6 0.00561551239591713 7 0.004831952526719392 8 0.006660258888180783 9 0.012275771284097915 10-11 0.002220086296060261 12-13 0.007313225445845565 14-15 0.007639708724677957 16-17 0.017434207089649698 18-19 0.01090454151300187 20-21 0.0027424595421920873 22-23 0.009206828463073434 24-25 0.01874014020497926 26-27 0.007639708724677957 28-29 0.016520053908919002 30-31 0.014299967612858741 32-33 0.011688101382199608 34-35 0.27300531775964565 36-37 0.4810404630316453 38-39 0.48019160650668113 40-41 0.4995847132693252 42-43 0.4892025450024552 44-45 0.5055920055998412 46-47 0.5120563745207226 48-49 0.518259556818538 50-51 0.5274010886258449 52-53 0.5169536237032084 54-55 0.5048084457306434 56-57 0.499976493203924 58-59 0.49723403366173197 60-61 0.49429568415224046 62-63 0.49220619116771314 64-65 0.5085303551093328 66-67 0.4811057596874118 68-69 0.5089874316996981 70-71 0.49096555470815006 72-73 0.4990623400231934 74-75 0.5115992979303572 76-77 0.4993888233020258 78-79 0.49756051694056436 80-81 0.5000417898596905 82-83 0.5006294597615889 84-85 0.501739502909619 86-87 0.5040901825172122 88-89 0.5164312504570766 90-91 0.5405910130906736 92-93 0.5309924046930012 94-95 0.5004988664500559 96-97 0.47385783089733274 98-99 0.46478159574579225 100-101 0.47294367771660206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 765736.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.82359572404141 #Duplication Level Percentage of deduplicated Percentage of total 1 71.32697295946284 11.999761572896363 2 10.052901516743692 3.3825190194259718 3 4.228837951064287 2.1343278021356755 4 2.555457735319486 1.7196795131555782 5 1.6226919354909843 1.3649756553681305 6 1.1697361747638206 1.1807501104807903 7 0.8657998051529638 1.0196106129893103 8 0.7159597662835304 0.9636014130106632 9 0.568553041644807 0.8608595868275684 >10 4.974163315995008 18.13655367948794 >50 0.8950850327335065 10.7391984564704 >100 0.9222323257540201 30.54406081608451 >500 0.07601242045743817 8.589897276911426 >1k 0.02482038219018389 6.634504272498141 >5k 7.756369434432466E-4 0.7297002122575301 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5592 0.7302777980922929 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4693 0.6128744110241651 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3941 0.5146682407513816 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2958 0.3862950155144854 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2492 0.32543853234012765 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2084 0.2721564612346814 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2040 0.2664103555272313 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 1597 0.20855751851813156 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1580 0.2063374322220713 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1527 0.1994159867108246 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 1453 0.18975208165738583 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1416 0.18492012913066647 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1399 0.18270004283460617 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1374 0.17943521004628227 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1352 0.17656215719255722 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1286 0.1679429986313821 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 1280 0.16715943876218434 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 1279 0.16702884545065141 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1263 0.1649393524661241 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1255 0.16389460597386044 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1219 0.159193246758674 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1210 0.15801790695487739 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1166 0.1522718012474273 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1159 0.1513576480666966 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1149 0.15005171495136707 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 1135 0.14822340858990565 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1121 0.14639510222844426 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1121 0.14639510222844426 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1117 0.14587272898231243 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1085 0.14169374301325782 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 1058 0.13816772360186802 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1032 0.13477229750201114 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 1002 0.13085449815602243 No Hit GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA 994 0.1298097516637588 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 960 0.1253695790716383 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 939 0.12262711952944617 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 921 0.12027643992185295 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 919 0.12001525329878705 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 917 0.11975406667572112 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 909 0.11870932018345748 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 904 0.11805635362579271 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 896 0.11701160713352904 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 893 0.11661982719893017 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 872 0.11387736765673809 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 869 0.11348558772213922 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 855 0.11165728136067782 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 843 0.11009016162228234 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 827 0.10800066863775504 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 825 0.10773948201468914 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 810 0.1057805823416948 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 804 0.10499702247249705 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 802 0.10473583584943115 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 794 0.10369108935716748 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 790 0.10316871611103565 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 787 0.10277693617643678 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 784 0.10238515624183792 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 775 0.1012098164380413 No Hit ATTAATAAGTGTCCTGCAGTAATGTTAGCTGTAAGCCGGACTGCTAATGC 774 0.10107922312650837 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 772 0.10081803650344244 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.9588996729943479E-4 0.0 12-13 0.0 0.0 0.0 3.9177993459886957E-4 0.0 14-15 0.0 0.0 0.0 3.9177993459886957E-4 0.0 16-17 0.0 0.0 0.0 7.18263213431261E-4 0.0 18-19 0.0 0.0 0.0 0.0011100431480301305 0.0 20-21 0.0 0.0 0.0 0.0018936030172278695 0.0 22-23 0.0 0.0 0.0 0.005354325772851217 0.0 24-25 0.0 0.0 0.0 0.009206828463073434 0.0 26-27 0.0 0.0 0.0 0.013516407743661 0.0 28-29 0.0 0.0 0.0 0.01730361377811674 0.0 30-31 0.0 0.0 0.0 0.031146504800610132 0.0 32-33 0.0 0.0 0.0 0.05739576041873439 0.0 34-35 0.0 0.0 0.0 0.09337421774606391 0.0 36-37 0.0 0.0 0.0 0.14646039888421075 0.0 38-39 0.0 0.0 0.0 0.22573053898471535 0.0 40-41 0.0 0.0 0.0 0.3177988236154497 0.0 42-43 0.0 0.0 0.0 0.40653697880209366 0.0 44-45 0.0 0.0 0.0 0.5064408621248054 0.0 46-47 0.0 0.0 0.0 0.6321369244752761 0.0 48-49 0.0 0.0 0.0 0.7689987149618145 0.0 50-51 0.0 0.0 0.0 0.9007020696428012 0.0 52-53 0.0 0.0 0.0 1.0243086390087446 0.0 54-55 0.0 0.0 0.0 1.1643699656278406 0.0 56-57 0.0 0.0 0.0 1.331137624455426 0.0 58-59 0.0 0.0 0.0 1.5077650783037497 0.0 60-61 0.0 0.0 0.0 1.6866779151039002 0.0 62-63 0.0 0.0 0.0 1.8625871057387924 0.0 64-65 0.0 0.0 0.0 2.056256986742167 0.0 66-67 0.0 0.0 0.0 2.2869500715651347 0.0 68-69 0.0 0.0 0.0 2.5365138899046147 0.0 70-71 0.0 0.0 0.0 2.8051443317279063 0.0 72-73 0.0 0.0 0.0 3.0625437487593636 0.0 74-75 0.0 0.0 0.0 3.341686952161058 0.0 76-77 0.0 0.0 0.0 3.6374155061274385 0.0 78-79 0.0 0.0 0.0 4.00059550550059 0.0 80-81 0.0 0.0 0.0 4.392440736755226 0.0 82-83 0.0 0.0 0.0 4.756273702686043 0.0 84-85 0.0 0.0 0.0 5.140544521871768 0.0 86-87 0.0 0.0 0.0 5.554460022775473 0.0 88-89 0.0 0.0 0.0 6.012516062977319 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATACG 15 6.709042E-4 94.67068 8 GATACGC 20 0.002102573 71.003 9 TATAACG 35 3.4857258E-6 67.62633 2 ACAACGC 25 0.005088803 56.806114 3 TAACGCA 45 1.5469614E-5 52.59482 4 GCGCTTA 40 5.4800644E-4 47.335335 8 AGAGCGC 40 5.4800644E-4 47.335335 5 CGCTTAT 40 5.4800644E-4 47.335335 9 TACCTGG 120 1.8189894E-12 43.39356 2 AGCGCTT 50 0.0016432735 37.86827 7 GAGCGCT 50 0.0016438009 37.865795 6 CATTCCG 165 0.0 37.29451 9 GTACAGA 80 1.101598E-5 35.50846 1 ACTCCCG 210 0.0 33.808746 6 GGTACTC 215 0.0 33.02681 3 TGGTACT 220 0.0 32.276203 2 TACTCCC 220 0.0 32.274094 5 GGATGCC 235 0.0 32.228313 8 AGGGGTG 385 0.0 31.96672 8 CTATACC 75 3.1408155E-4 31.55689 4 >>END_MODULE