FastQCFastQC Report
Thu 26 May 2016
SRR936293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082910
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31390.15070262277294746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22870.10979831101679861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43800.041.1882741
GGTATCA27650.040.0722241
TCAACGC50800.034.850224
ATCAACG51450.034.5027663
CAACGCA52300.033.760185
TATCAAC53000.033.5822332
AACGCAG54400.032.541616
ACGCAGA67150.026.3640887
GGACCGT2007.4942363E-1026.0332876
CGCAGAG68600.025.7384478
TACATGG70550.025.4296172
GTACATG73500.025.1246511
ACATGGG68600.024.9109973
CGCGGAA4300.023.23086282-83
CATGGGG47550.022.7972564
ATTCGCG4700.022.77106980-81
TCGCGCT4700.022.2639760-61
CTCGCGC4950.022.10041660-61
GCAGAGT81000.021.4475969
GTACCTG20300.020.7594931
Produced by FastQC (version 0.11.5)