##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936293_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2082910 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56439836574792 33.0 31.0 34.0 30.0 34.0 2 31.65917202375523 33.0 31.0 34.0 30.0 34.0 3 31.72466117114998 34.0 31.0 34.0 30.0 34.0 4 35.21101103744281 37.0 35.0 37.0 33.0 37.0 5 35.18625192639144 37.0 35.0 37.0 33.0 37.0 6 35.272402552198606 37.0 35.0 37.0 33.0 37.0 7 35.210633680763934 37.0 35.0 37.0 33.0 37.0 8 35.206461633003826 37.0 35.0 37.0 33.0 37.0 9 36.86231186177031 39.0 37.0 39.0 33.0 39.0 10-11 36.77433398466569 39.0 37.0 39.0 33.0 39.0 12-13 36.6710974069931 39.0 37.0 39.0 33.0 39.0 14-15 37.909159061121215 40.0 38.0 41.0 33.0 41.0 16-17 37.8465104109155 40.0 38.0 41.0 33.0 41.0 18-19 37.77514390924236 40.0 38.0 41.0 32.0 41.0 20-21 37.68520771420753 40.0 38.0 41.0 32.0 41.0 22-23 37.52379483511049 40.0 38.0 41.0 32.0 41.0 24-25 37.67801993365052 40.0 38.0 41.0 32.0 41.0 26-27 37.628551161596036 40.0 38.0 41.0 32.0 41.0 28-29 37.49066642341724 40.0 38.0 41.0 32.0 41.0 30-31 37.34254672549462 40.0 37.5 41.0 31.5 41.0 32-33 37.166206893240705 40.0 37.0 41.0 31.0 41.0 34-35 36.90272911455608 40.0 37.0 41.0 30.5 41.0 36-37 36.708016428938365 40.0 37.0 41.0 30.0 41.0 38-39 36.54084429956167 40.0 36.0 41.0 30.0 41.0 40-41 36.35381005420301 40.0 36.0 41.0 30.0 41.0 42-43 36.165933477682664 40.0 35.5 41.0 29.0 41.0 44-45 35.98824505139444 39.0 35.0 41.0 28.5 41.0 46-47 35.730129002213246 39.0 35.0 41.0 27.5 41.0 48-49 35.56813736551267 39.0 35.0 41.0 27.0 41.0 50-51 35.26187857372618 38.5 35.0 40.5 26.5 41.0 52-53 35.275847732259194 39.0 35.0 40.5 27.0 41.0 54-55 35.34488336029881 39.0 35.0 41.0 27.0 41.0 56-57 35.17193085635001 39.0 35.0 41.0 26.0 41.0 58-59 34.93115209010471 38.5 35.0 41.0 26.0 41.0 60-61 34.63316873988795 38.0 34.0 40.0 26.0 41.0 62-63 34.31474067530522 37.5 34.0 40.0 25.5 41.0 64-65 33.9756748971391 37.0 34.0 40.0 24.5 41.0 66-67 33.61981482637272 36.0 34.0 39.5 23.5 41.0 68-69 33.236046204588774 36.0 33.0 39.0 22.5 41.0 70-71 32.83265551560077 35.0 33.0 39.0 22.0 40.5 72-73 32.42730818902401 35.0 33.0 37.5 20.5 40.0 74-75 32.008223831082475 35.0 32.0 37.0 19.5 39.0 76-77 31.613180838346352 35.0 32.0 36.5 18.0 39.0 78-79 31.223308976384 35.0 32.0 36.0 17.0 39.0 80-81 30.834549500458493 35.0 31.0 36.0 12.0 37.0 82-83 30.489104426019367 35.0 31.0 35.0 7.5 37.0 84-85 30.17705301717309 34.5 31.0 35.0 6.5 36.5 86-87 29.856135166665865 34.0 31.0 35.0 2.0 36.0 88-89 29.530936766350923 34.0 30.0 35.0 2.0 36.0 90-91 29.1811326461537 34.0 30.0 35.0 2.0 35.5 92-93 28.86403037097138 34.0 29.5 35.0 2.0 35.0 94-95 28.484415793289195 34.0 29.0 35.0 2.0 35.0 96-97 28.056742730122764 34.0 29.0 35.0 2.0 35.0 98-99 27.550968836867654 34.0 29.0 35.0 2.0 35.0 100-101 24.303765165081543 30.0 14.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3917339161706792 1101 2 0.34057784071733366 1101 3 0.35226873494818634 1101 4 0.13154989897295621 1101 5 0.49965787938670303 1101 6 0.39225736522410415 1101 7 0.47887566034289364 1101 8 0.44818144438985286 1101 9 0.5251938303579919 1101 10-11 0.6554691871705032 1101 12-13 0.5355286200760148 1101 14-15 0.683148079374817 1101 16-17 0.7295326004067562 1101 18-19 0.7693122751547179 1101 20-21 0.7423796105663385 1101 22-23 0.6779386692071725 1101 24-25 0.6789804206544829 1101 26-27 0.6053527046018488 1101 28-29 0.5990689349236291 1101 30-31 0.6079531087757815 1101 32-33 0.793075310598347 1101 34-35 0.5076441308607258 1101 36-37 0.6571301663571845 1101 38-39 0.7189838273501294 1101 40-41 0.5903715827003069 1101 42-43 0.6355533515172382 1101 44-45 0.6236906109255358 1101 46-47 0.5544379552371126 1101 48-49 0.7280987904945917 1101 50-51 0.7778544267682932 1101 52-53 0.8188559025901085 1101 54-55 0.8338632876570955 1101 56-57 0.8879312120371665 1101 58-59 0.8571906683187791 1101 60-61 0.7309870113761789 1101 62-63 0.7173183143242028 1101 64-65 0.7516877895648051 1101 66-67 0.9247346254175639 1101 68-69 0.9770770049251851 1101 70-71 1.0923899000473583 1101 72-73 0.9745217489011395 1101 74-75 0.7602304700250855 1101 76-77 0.8840545782543678 1101 78-79 1.0199148147634567 1101 80-81 0.9208937077011328 1101 82-83 1.0794780657162768 1101 84-85 0.9786661275783359 1101 86-87 0.9752359131493407 1101 88-89 0.9295090709085976 1101 90-91 0.9805437083803348 1101 92-93 1.0183881279989002 1101 94-95 1.1260261851442586 1101 96-97 1.069990468571703 1101 98-99 1.2706488688501096 1101 100-101 1.5071002685261377 1102 1 0.1906514265935506 1102 2 0.18260732255176038 1102 3 0.15725980105897008 1102 4 0.22676580129576251 1102 5 0.21105901101267222 1102 6 0.09302121275239728 1102 7 0.031581139794951696 1102 8 0.13382617339521374 1102 9 0.1934800126093279 1102 10-11 0.19511480956479232 1102 12-13 0.2449159041736877 1102 14-15 0.29333718002997244 1102 16-17 0.15507756764915825 1102 18-19 0.18286796306418296 1102 20-21 0.16038139734418166 1102 22-23 0.21439042620538373 1102 24-25 0.3945670793799252 1102 26-27 0.42872226981923944 1102 28-29 0.17419252039116628 1102 30-31 0.34512999978233694 1102 32-33 0.015008008632882763 1102 34-35 -0.03262835276047582 1102 36-37 0.1479751633792219 1102 38-39 0.05928757958361075 1102 40-41 0.12705859878131065 1102 42-43 -0.015187416797232345 1102 44-45 0.08701774016913078 1102 46-47 0.1913445960947726 1102 48-49 0.19961606268041265 1102 50-51 0.09548843510658855 1102 52-53 0.20786721884978476 1102 54-55 0.2395377962931775 1102 56-57 0.36994505956544543 1102 58-59 0.4280624670084734 1102 60-61 0.2332941882671875 1102 62-63 0.29523671813599606 1102 64-65 0.10135202422829792 1102 66-67 0.2780960012364986 1102 68-69 0.30621414012470893 1102 70-71 0.32246211565188077 1102 72-73 0.16055510208756374 1102 74-75 0.23596751588571863 1102 76-77 0.26167295228653487 1102 78-79 0.3261491804573282 1102 80-81 0.25745788280536175 1102 82-83 0.33330623724695485 1102 84-85 0.45647881370102894 1102 86-87 0.34675467431669915 1102 88-89 0.24858813582819295 1102 90-91 0.13561666847563103 1102 92-93 0.16706814586668273 1102 94-95 0.3850963161746357 1102 96-97 0.4462776931101189 1102 98-99 0.2512950868370041 1102 100-101 0.2818307628680081 1103 1 0.3764175287722189 1103 2 0.29564723312164176 1103 3 0.377781159635699 1103 4 0.2594708246046764 1103 5 0.3091740809394139 1103 6 0.3216233914223636 1103 7 0.4147594767752594 1103 8 0.374832024408434 1103 9 0.2876278101547598 1103 10-11 0.5056276056831379 1103 12-13 0.473304615999389 1103 14-15 0.5330110930734477 1103 16-17 0.5528917623023801 1103 18-19 0.43954668059819824 1103 20-21 0.6518569943378054 1103 22-23 0.5360974474212483 1103 24-25 0.5811882381659501 1103 26-27 0.5744993129169487 1103 28-29 0.597452676418186 1103 30-31 0.6150543647333464 1103 32-33 0.4949195298963289 1103 34-35 0.3999196921448558 1103 36-37 0.4447291591931304 1103 38-39 0.7318567927888182 1103 40-41 0.5482494940339038 1103 42-43 0.4686646191650894 1103 44-45 0.5263188295041203 1103 46-47 0.6646576728714777 1103 48-49 0.7616974536042065 1103 50-51 0.7443146647707479 1103 52-53 0.7160419881390965 1103 54-55 0.6622485279076784 1103 56-57 0.7359515051938388 1103 58-59 0.7674646409215171 1103 60-61 0.6966593533323575 1103 62-63 0.6272056078705646 1103 64-65 0.6519484232388564 1103 66-67 0.7919321015124083 1103 68-69 0.7827238680288104 1103 70-71 0.7371199740858998 1103 72-73 0.6439201482297037 1103 74-75 0.7747661554062191 1103 76-77 0.6706320189785693 1103 78-79 0.6777891290825764 1103 80-81 0.7029263206324003 1103 82-83 0.9269117457618421 1103 84-85 0.8757312635155081 1103 86-87 0.9751368453755909 1103 88-89 0.8063805675663716 1103 90-91 0.8939829038480944 1103 92-93 0.879774477328958 1103 94-95 0.7036726885464333 1103 96-97 0.8149979623518675 1103 98-99 0.9450349783791978 1103 100-101 1.0994420748269071 1104 1 0.22428611443304192 1104 2 0.22070251937121554 1104 3 0.21735040966216346 1104 4 0.24337150362801196 1104 5 0.3018117905240345 1104 6 0.28457375187358736 1104 7 0.19437353745576758 1104 8 0.26217516676136654 1104 9 0.37329619502848743 1104 10-11 0.2913928069297427 1104 12-13 0.2623145762491319 1104 14-15 0.37485054017604824 1104 16-17 0.3757545895847656 1104 18-19 0.30662498407521355 1104 20-21 0.3534696020456636 1104 22-23 0.48858864408209257 1104 24-25 0.41922671925694743 1104 26-27 0.3946668991792066 1104 28-29 0.47494113930340376 1104 30-31 0.3754422758612037 1104 32-33 0.4206473540319635 1104 34-35 0.47255014849925914 1104 36-37 0.3391138095664985 1104 38-39 0.18598360158855343 1104 40-41 0.3918151459463246 1104 42-43 0.3723838045697576 1104 44-45 0.28490724736927575 1104 46-47 0.2266818068753409 1104 48-49 0.29418561402913923 1104 50-51 0.12325610031704315 1104 52-53 0.1363546099229893 1104 54-55 0.18538557615126194 1104 56-57 0.25265589214756545 1104 58-59 0.24021849525432515 1104 60-61 0.12606875891646752 1104 62-63 0.25654256840066125 1104 64-65 0.34186686947712275 1104 66-67 0.33281033565621243 1104 68-69 0.18653262820921412 1104 70-71 0.1956555777326443 1104 72-73 0.3097114250425079 1104 74-75 0.24436817002435873 1104 76-77 0.277969065593517 1104 78-79 0.2554770703401381 1104 80-81 0.3408147639740271 1104 82-83 0.4490671068121692 1104 84-85 0.23655445570458866 1104 86-87 0.25989985611176536 1104 88-89 0.3356904230019886 1104 90-91 0.40096610101613805 1104 92-93 0.4110689375362355 1104 94-95 0.21295109392929845 1104 96-97 0.39923504119046527 1104 98-99 0.4727678233555075 1104 100-101 0.4768585579816147 1105 1 0.230021622910634 1105 2 0.27960537290450205 1105 3 0.2477617507062284 1105 4 0.12780063574479072 1105 5 0.04635636039397184 1105 6 0.12141478229732883 1105 7 0.22256740210703185 1105 8 0.24797323089290302 1105 9 0.1866503306384928 1105 10-11 0.22654526463274038 1105 12-13 0.33084054974359134 1105 14-15 0.35182913350890743 1105 16-17 0.3522139528002839 1105 18-19 0.40083176498903583 1105 20-21 0.34209697423692376 1105 22-23 0.5173687096142245 1105 24-25 0.34936794488806555 1105 26-27 0.3170167792519294 1105 28-29 0.3132956263032227 1105 30-31 0.40336246986144886 1105 32-33 0.37109643020747995 1105 34-35 0.22654710422332158 1105 36-37 0.28880801775297016 1105 38-39 0.17514508628903513 1105 40-41 0.2863428077654646 1105 42-43 0.359926829151334 1105 44-45 0.5642394083008497 1105 46-47 0.5019221368345015 1105 48-49 0.44064571309626643 1105 50-51 0.3935773863541314 1105 52-53 0.3759392092931364 1105 54-55 0.3648767306362757 1105 56-57 0.5213913766479692 1105 58-59 0.7346756310718519 1105 60-61 0.6037881646460264 1105 62-63 0.5074501876065511 1105 64-65 0.5538909326512282 1105 66-67 0.49310279119300304 1105 68-69 0.4548092389594274 1105 70-71 0.5987703796769424 1105 72-73 0.6583248408573752 1105 74-75 0.6333785984470808 1105 76-77 0.6244745559559313 1105 78-79 0.5637720195043663 1105 80-81 0.6646787620624686 1105 82-83 0.5307156293627138 1105 84-85 0.6136837745953869 1105 86-87 0.6805523167085674 1105 88-89 0.48304181198389173 1105 90-91 0.6016344857722693 1105 92-93 0.5419550268818476 1105 94-95 0.4813971985872385 1105 96-97 0.5343700534229647 1105 98-99 0.5179949989338333 1105 100-101 0.7105365013673399 1106 1 0.05808897844681482 1106 2 0.11517313415343722 1106 3 0.089862890952908 1106 4 0.05155978968063124 1106 5 0.03252710303679862 1106 6 -0.10311053377763812 1106 7 -0.020230360978644057 1106 8 -0.055245120638019785 1106 9 -0.10775409506607758 1106 10-11 -0.0227647563252944 1106 12-13 -0.024465304203552307 1106 14-15 -0.02010907196007139 1106 16-17 0.04300388556292489 1106 18-19 0.012820650464682615 1106 20-21 0.029866452392731446 1106 22-23 0.09781560054445748 1106 24-25 0.14672865975528282 1106 26-27 0.02041201352397337 1106 28-29 0.0024873047937035153 1106 30-31 0.10371465794320045 1106 32-33 -0.023888666031325556 1106 34-35 -0.1403550496234942 1106 36-37 0.03349759201792324 1106 38-39 -0.04986213900733105 1106 40-41 0.0171540648440498 1106 42-43 0.042846335473100794 1106 44-45 0.09876362554258833 1106 46-47 0.13659497728632886 1106 48-49 0.10504439866713966 1106 50-51 0.12761923986924018 1106 52-53 0.1332624248053449 1106 54-55 0.1406979079598898 1106 56-57 0.14908729446914748 1106 58-59 0.14588135056565932 1106 60-61 0.17887951351328013 1106 62-63 0.1638943632690797 1106 64-65 0.14735806162991594 1106 66-67 0.1913697360168669 1106 68-69 0.3710706607426886 1106 70-71 0.27497363548505405 1106 72-73 0.229338876780929 1106 74-75 0.22939336291578627 1106 76-77 0.3274869364110238 1106 78-79 0.31392274145919075 1106 80-81 0.38253033723054486 1106 82-83 0.3358644250174301 1106 84-85 0.265531832962413 1106 86-87 0.19912366774675405 1106 88-89 0.23740058397492803 1106 90-91 0.26117303998043795 1106 92-93 0.2462588705791049 1106 94-95 0.15781407185326657 1106 96-97 0.2632346913386989 1106 98-99 0.23302984478036493 1106 100-101 0.5266625340633801 1107 1 -0.11266863367334778 1107 2 0.05802046169176123 1107 3 0.10245997325010237 1107 4 -0.03142702885681814 1107 5 -0.09817294479160665 1107 6 0.04404180394195123 1107 7 -0.05129839815884196 1107 8 0.04876435199567908 1107 9 0.05542756606032384 1107 10-11 -0.09327065941689483 1107 12-13 0.05051181702584273 1107 14-15 0.07949074103824216 1107 16-17 -0.13656637935423532 1107 18-19 -0.023192208170328854 1107 20-21 0.1573644652161903 1107 22-23 0.1759218510870042 1107 24-25 0.1610759041754406 1107 26-27 0.08423059239063235 1107 28-29 0.16510884028402728 1107 30-31 0.06177275101398294 1107 32-33 0.15284820569607405 1107 34-35 -0.0832172703478875 1107 36-37 0.15270832691297187 1107 38-39 0.1660460815207614 1107 40-41 0.12309880062758083 1107 42-43 -0.22445614258285218 1107 44-45 0.18263254621495406 1107 46-47 0.2697163734898993 1107 48-49 0.10242433263784534 1107 50-51 0.1360639600503859 1107 52-53 0.18053665970691668 1107 54-55 0.37768112149527155 1107 56-57 0.13256493568349725 1107 58-59 0.32797069152657343 1107 60-61 0.23809311924724597 1107 62-63 0.39555120610653205 1107 64-65 0.2425687817736204 1107 66-67 0.22787747394898616 1107 68-69 0.25475260810194555 1107 70-71 0.2457697996811561 1107 72-73 0.06755036683142635 1107 74-75 0.1643377985661587 1107 76-77 0.2901702857155257 1107 78-79 0.22239744658828542 1107 80-81 0.1600045776922414 1107 82-83 0.18531573167465254 1107 84-85 0.12115870560326059 1107 86-87 -0.11015688640458876 1107 88-89 -0.03463608333285251 1107 90-91 0.2908906197838057 1107 92-93 -0.02493782103436004 1107 94-95 0.0071107730551034365 1107 96-97 0.06886910986048989 1107 98-99 0.04641992540781814 1107 100-101 0.1786063643411957 1108 1 0.004912393449753694 1108 2 0.0813757808139286 1108 3 0.1406706068445409 1108 4 0.15444697873290636 1108 5 0.17212342845586903 1108 6 0.035533320075167296 1108 7 0.06829794735337913 1108 8 0.1422988670583223 1108 9 0.2145113511171033 1108 10-11 0.14878322026246593 1108 12-13 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-1.8465573720103095 2316 54-55 -1.9052752733177698 2316 56-57 -2.0110072727770145 2316 58-59 -2.0839628572001914 2316 60-61 -1.9947896731642274 2316 62-63 -2.0287604399341035 2316 64-65 -1.8395119469604069 2316 66-67 -1.9659043009227126 2316 68-69 -2.091079289137369 2316 70-71 -1.8679502500097662 2316 72-73 -1.7998146754579238 2316 74-75 -1.8055569181782403 2316 76-77 -1.9527471878500116 2316 78-79 -2.0827167425165705 2316 80-81 -1.9504372516146553 2316 82-83 -2.0289569352614585 2316 84-85 -2.1026696689697317 2316 86-87 -2.1662087725849446 2316 88-89 -1.994801314732026 2316 90-91 -2.167859387448324 2316 92-93 -2.1031840754118605 2316 94-95 -2.0506648392168643 2316 96-97 -2.2618044533636024 2316 98-99 -1.9874245747446757 2316 100-101 -1.9161043463992975 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23434.0 3 4745.0 4 3842.0 5 3218.0 6 3045.0 7 3255.0 8 3784.0 9 4441.0 10 5232.0 11 6307.0 12 7781.0 13 10799.0 14 11028.0 15 9636.0 16 10815.0 17 12650.0 18 11976.0 19 9968.0 20 9756.0 21 10474.0 22 10856.0 23 12223.0 24 13833.0 25 16096.0 26 18764.0 27 22297.0 28 27236.0 29 32394.0 30 39707.0 31 48724.0 32 61912.0 33 81224.0 34 112526.0 35 168758.0 36 281004.0 37 452308.0 38 435434.0 39 81336.0 40 92.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.632632476374596 17.252800826138643 12.560333337335944 24.554233360150818 2 16.042269264897378 18.509608920950633 40.06361132558166 25.38451048857032 3 20.335575625753233 22.335681258372585 27.786516476287876 29.542226639586307 4 12.937717316258833 14.617384148408341 35.26344900167756 37.181449533655275 5 14.593812357999013 36.737371875018006 33.54416218697365 15.124653580009333 6 35.58765250599317 33.839466061579984 16.301378873595812 14.271502558831028 7 31.302615621131995 30.740678146758633 19.19916227913082 18.757543952978548 8 27.65125471020477 35.202322740734196 18.338109157675184 18.80831339138585 9 26.885079023626663 15.968366540942714 18.8598894173403 38.286665018090325 10-11 26.49069847553181 25.48321236570433 26.283147070790967 21.742942087972892 12-13 26.72479584965316 22.281562429854265 27.117744005127914 23.875897715364662 14-15 23.212862011141667 24.184618930270354 24.58959366886371 28.01292538972427 16-17 23.921453570409028 27.290123209285316 25.310015997495334 23.478407222810322 18-19 23.74955015471797 26.11954756908291 26.827464197837337 23.303438078361786 20-21 24.179480974905275 25.443267518969403 27.07057423848729 23.306677267638037 22-23 23.778753430629738 25.373960247908013 27.016797361896145 23.830488959566104 24-25 23.60102119780173 25.56346315037073 27.2058023781049 23.629713273722643 26-27 23.593478536946403 25.782719846587703 26.951509144024964 23.67229247244093 28-29 23.357528065393 26.070095896995362 27.10056483719215 23.471811200419488 30-31 23.56191062452013 25.722135765413253 26.991218218319634 23.72473539174698 32-33 23.299863706281634 25.709792092659562 27.158894179463562 23.831450021595245 34-35 23.76483188524393 25.887843269392764 26.95489662807769 23.392428217285616 36-37 23.92063177401143 25.708215946476898 26.68211084599086 23.689041433520817 38-39 23.58605571492134 25.892329823266763 26.85187663761909 23.66973782419281 40-41 23.761923438176133 25.72925154118866 27.039460893920776 23.469364126714435 42-43 23.758885517941312 25.68232746363971 26.80072152588398 23.758065492534996 44-45 23.674050181009797 25.878925095136278 26.87327288095036 23.57375184290357 46-47 23.93768047068927 26.05493787104291 26.517666527556983 23.489715130710835 48-49 24.03351151231471 26.097233758648503 26.402004495446775 23.467250233590015 50-51 23.772204453726527 26.177731174348512 26.409330165243794 23.64073420668117 52-53 24.033363360422044 26.040240802133386 26.392387626394825 23.53400821104974 54-55 23.87941901679394 26.011696081349612 26.382951625096307 23.72593327676014 56-57 23.715759764601284 26.32791075920621 26.311846374309333 23.644483101883175 58-59 23.902585391185468 26.082303472618694 26.32714488287241 23.687966253323424 60-61 23.72499655091572 26.139958533806336 26.509155268608897 23.62588964666905 62-63 23.673553163875834 26.184519406396273 26.620298962913154 23.52162846681474 64-65 23.619389933541594 26.207432244991498 26.457175939885897 23.71600188158101 66-67 23.653390306093534 26.326725660387464 26.344909482429095 23.674974551089907 68-69 23.656048340310072 26.119789851169102 26.575856910829554 23.648304897691272 70-71 23.636623676443136 25.973599047227182 26.459729285560275 23.93004799076941 72-73 23.6883002107403 26.204448243682073 26.343623194313558 23.763628351264067 74-75 23.613268848871297 26.47490355314275 26.171088715669455 23.7407388823165 76-77 23.843535856165293 26.327140021274182 26.07488898590646 23.754435136654067 78-79 23.82328388103759 26.406401920363848 26.17079457900504 23.59951961959352 80-81 23.776262066240673 26.432131519400176 26.154522655792267 23.63708375856688 82-83 23.8790456978516 26.546777878255966 26.162285975621668 23.411890448270764 84-85 23.843415940909637 26.51342833421696 25.926036703293416 23.717119021579986 86-87 23.745939440954462 26.367540324574563 26.23762173234599 23.648898502124986 88-89 23.805182536128953 26.527374629446644 25.99849173202816 23.668951102396246 90-91 23.815886153456358 26.67114685070008 25.981069814860618 23.53189718098295 92-93 23.79303780472181 26.428006671747927 26.142746742118316 23.63620878141195 94-95 23.73113447617945 26.313296138974717 26.322630909911016 23.632938474934814 96-97 23.84901974969254 26.14941281439147 26.182690684607778 23.818876751308206 98-99 23.567481023524078 26.19342020235144 26.138058082001525 24.101040692122954 100-101 23.766852909756903 26.263675845388494 25.9947286051542 23.974742639700402 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 33.0 1 38.5 2 87.0 3 148.0 4 126.5 5 120.5 6 231.0 7 429.0 8 698.5 9 875.0 10 876.0 11 883.5 12 1031.0 13 1222.5 14 1406.5 15 1591.0 16 1750.0 17 1871.0 18 2036.5 19 2136.5 20 2215.0 21 2468.0 22 2735.5 23 3129.0 24 3877.5 25 4904.5 26 6457.0 27 8494.0 28 10687.5 29 13331.0 30 16324.0 31 19782.0 32 24266.5 33 27538.0 34 30070.5 35 33735.5 36 38375.5 37 42854.5 38 46621.5 39 50182.5 40 53557.0 41 57511.0 42 60157.0 43 62885.0 44 66842.5 45 71400.0 46 74913.0 47 77078.5 48 79496.5 49 80368.0 50 79112.5 51 78571.0 52 79867.5 53 81068.0 54 83111.5 55 80560.5 56 73320.0 57 67311.5 58 63404.5 59 58042.0 60 50216.0 61 42874.0 62 36226.0 63 28991.5 64 21931.0 65 16694.5 66 12106.0 67 9581.5 68 7425.5 69 5531.0 70 4459.0 71 3116.5 72 2373.0 73 1995.0 74 1571.5 75 1168.0 76 790.0 77 469.0 78 301.5 79 212.0 80 145.5 81 88.0 82 58.0 83 41.5 84 34.0 85 30.0 86 24.0 87 22.5 88 28.5 89 25.5 90 20.0 91 22.5 92 17.5 93 12.5 94 14.0 95 16.0 96 19.0 97 21.0 98 16.5 99 12.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044889121469482596 2 0.012578555962571594 3 0.010562146228113552 4 0.005569131647550782 5 0.00854573649365551 6 0.005905199936627123 7 0.005569131647550782 8 0.009505931605302198 9 0.01464297545261197 10-11 0.0025205121680725523 12-13 0.009241877949599358 14-15 0.009001829171687688 16-17 0.019035868088395563 18-19 0.011978434017792415 20-21 0.003936799957751415 22-23 0.009697970627631535 24-25 0.02177242415658862 26-27 0.0075135267486353225 28-29 0.017427541276387362 30-31 0.0145469559414473 32-33 0.013226687662933109 34-35 0.26213326547954546 36-37 0.46267001454695594 38-39 0.46029353164563036 40-41 0.4799535265565963 42-43 0.47066363885141466 44-45 0.48461047284808273 46-47 0.48993955571772185 48-49 0.4977891507554335 50-51 0.5078471945499325 52-53 0.49627684345458994 54-55 0.4838903265143477 56-57 0.4801695704567168 58-59 0.47700092658828275 60-61 0.4751045412427805 62-63 0.4725840290747079 64-65 0.48897936060607516 66-67 0.4627420291803294 68-69 0.4891473947506133 70-71 0.47063963397362346 72-73 0.4791133558339054 74-75 0.49286815080824425 76-77 0.478897311933785 78-79 0.4786332582780821 80-81 0.48151384361302213 82-83 0.48223398994675715 84-85 0.480913721668243 86-87 0.4836262728586449 88-89 0.496132814187843 90-91 0.5195855797898133 92-93 0.5083993067391294 94-95 0.48081770215707825 96-97 0.4541722878088828 98-99 0.4460106293598859 100-101 0.4539322390309711 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2082910.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.69804869763894 #Duplication Level Percentage of deduplicated Percentage of total 1 75.50768774544417 42.056308690918634 2 13.576598890028427 15.123801322502278 3 4.6442843612771565 7.76032729560294 4 2.1329145994933985 4.751967249219534 5 1.1422054660161767 3.1809307834439196 6 0.6727793318990438 2.248349759452676 7 0.45954634305824804 1.791708421613613 8 0.3336706651694915 1.4867843966067131 9 0.22957058880053471 1.1507970451102043 >10 1.1767303651816299 11.802386227202705 >50 0.08196088153506859 3.149880559074434 >100 0.0387714001881554 3.8179271102010066 >500 0.0023300729349993022 0.8834688446120521 >1k 9.492889735182145E-4 0.7953622944393578 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3139 0.15070262277294746 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2287 0.10979831101679861 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.800975558233433E-5 0.0 8 0.0 0.0 0.0 4.800975558233433E-5 0.0 9 0.0 0.0 0.0 4.800975558233433E-5 4.800975558233433E-5 10-11 0.0 0.0 0.0 4.800975558233433E-5 4.800975558233433E-5 12-13 0.0 0.0 0.0 4.800975558233433E-5 1.2002438895583582E-4 14-15 0.0 0.0 0.0 4.800975558233433E-5 1.4402926674700298E-4 16-17 0.0 0.0 0.0 4.800975558233433E-5 1.9203902232933732E-4 18-19 0.0 0.0 0.0 9.601951116466866E-5 2.4004877791167166E-4 20-21 0.0 0.0 0.0 1.4402926674700298E-4 4.5609267803217615E-4 22-23 0.0 0.0 0.0 1.6803414453817015E-4 4.800975558233433E-4 24-25 0.0 0.0 0.0 2.640536557028388E-4 4.800975558233433E-4 26-27 0.0 0.0 0.0 3.8407804465867464E-4 5.041024336145105E-4 28-29 0.0 0.0 0.0 5.521121891968448E-4 5.281073114056776E-4 30-31 0.0 0.0 0.0 0.001728351200964036 5.761170669880119E-4 32-33 0.0 0.0 0.0 0.0038167755687955793 6.241268225703463E-4 34-35 0.0 0.0 0.0 0.006865395048273809 6.721365781526806E-4 36-37 0.0 0.0 0.0 0.011834404751045413 6.721365781526806E-4 38-39 0.0 0.0 0.0 0.021316331478556442 7.20146333735015E-4 40-41 0.0 0.0 0.0 0.032478599651449176 7.20146333735015E-4 42-43 2.4004877791167165E-5 0.0 0.0 0.0432807946574744 7.20146333735015E-4 44-45 4.800975558233433E-5 0.0 0.0 0.05497117014177281 7.441512115261822E-4 46-47 4.800975558233433E-5 0.0 0.0 0.0706223504616138 7.681560893173493E-4 48-49 4.800975558233433E-5 0.0 0.0 0.088169916126957 7.681560893173493E-4 50-51 4.800975558233433E-5 0.0 0.0 0.11023039881703962 7.681560893173493E-4 52-53 4.800975558233433E-5 0.0 0.0 0.13068255469511403 8.64175600482018E-4 54-55 4.800975558233433E-5 0.0 0.0 0.15420733493045785 8.64175600482018E-4 56-57 4.800975558233433E-5 0.0 0.0 0.18178893951250896 8.64175600482018E-4 58-59 4.800975558233433E-5 0.0 0.0 0.21541977329793416 8.64175600482018E-4 60-61 9.601951116466866E-5 0.0 0.0 0.25013082658396185 9.121853560643523E-4 62-63 9.601951116466866E-5 0.0 0.0 0.28570605547047156 9.121853560643523E-4 64-65 9.601951116466866E-5 0.0 0.0 0.3232976940914394 9.121853560643523E-4 66-67 9.601951116466866E-5 0.0 0.0 0.36924303018373333 9.121853560643523E-4 68-69 9.601951116466866E-5 0.0 0.0 0.42181371254638944 9.121853560643523E-4 70-71 9.601951116466866E-5 0.0 0.0 0.48312217042503036 9.121853560643523E-4 72-73 9.601951116466866E-5 0.0 0.0 0.5457989063377678 0.001008204867229021 74-75 9.601951116466866E-5 0.0 0.0 0.610732100762875 0.0010562146228113552 76-77 9.601951116466866E-5 0.0 0.0 0.6811624122021596 0.0010802195006025224 78-79 9.601951116466866E-5 0.0 0.0 0.7661636844606824 0.0011042243783936896 80-81 9.601951116466866E-5 0.0 0.0 0.8636234882928211 0.0011042243783936896 82-83 9.601951116466866E-5 0.0 0.0 0.9676606286397396 0.0012002438895583583 84-85 9.601951116466866E-5 0.0 0.0 1.0713617006975817 0.0012002438895583583 86-87 9.601951116466866E-5 0.0 0.0 1.1847607433830554 0.0012242487673495253 88-89 9.601951116466866E-5 0.0 0.0 1.3118905761650765 0.0012482536451406926 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4380 0.0 41.188274 1 GGTATCA 2765 0.0 40.072224 1 TCAACGC 5080 0.0 34.85022 4 ATCAACG 5145 0.0 34.502766 3 CAACGCA 5230 0.0 33.76018 5 TATCAAC 5300 0.0 33.582233 2 AACGCAG 5440 0.0 32.54161 6 ACGCAGA 6715 0.0 26.364088 7 GGACCGT 200 7.4942363E-10 26.033287 6 CGCAGAG 6860 0.0 25.738447 8 TACATGG 7055 0.0 25.429617 2 GTACATG 7350 0.0 25.124651 1 ACATGGG 6860 0.0 24.910997 3 CGCGGAA 430 0.0 23.230862 82-83 CATGGGG 4755 0.0 22.797256 4 ATTCGCG 470 0.0 22.771069 80-81 TCGCGCT 470 0.0 22.26397 60-61 CTCGCGC 495 0.0 22.100416 60-61 GCAGAGT 8100 0.0 21.447596 9 GTACCTG 2030 0.0 20.759493 1 >>END_MODULE