FastQCFastQC Report
Thu 26 May 2016
SRR936293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2082910
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33480.16073666168965534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25900.12434526695824591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.049.402521
GTATCAA41500.040.6917461
ATCAACG50750.032.5249373
TCAACGC50900.032.4298674
TATCAAC52700.031.5082682
CAACGCA53050.031.294385
AACGCAG55550.030.2275456
ACATGGG64400.026.0729793
TACATGG67750.025.6294252
ACGCAGA65800.025.3746747
GTACATG70850.024.842081
CGCAGAG67650.024.540538
TGGTATC13100.022.8158342
GTACCTG19450.022.7451821
GTGGTAT14500.022.636391
GTATAGC8000.022.0007671
CGTTACG550.002901567621.56138434-35
GAGTACA51700.021.5303651
TACCTGG19950.021.1648032
CATGGGG44350.020.9626464
Produced by FastQC (version 0.11.5)