FastQCFastQC Report
Thu 26 May 2016
SRR936292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594312
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43190.16647959073542426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29550.11390303093845304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35900.043.248931
GTATCAA60250.039.5976561
ATCAACG71550.032.8751223
TCAACGC72100.032.6893584
TATACCG1950.031.5515755
CAACGCA74700.031.5515735
TATCAAC77000.030.6699942
AACGCAG78450.030.2214436
TACATGG92850.027.5752372
GTACATG96050.027.2043111
ACGCAGA91200.025.9450157
ACATGGG96200.025.5336533
CGCAGAG94350.024.9297778
ATACCGT2453.1286618E-1023.1785136
CATGGGG61100.023.0052814
ATACCGA1954.3804903E-721.8412916
GCAGAGT111750.020.5814469
GAGTACA74050.019.177511
GTGTAGC17050.019.1567021
CAGAGTA99650.018.68890810-11
Produced by FastQC (version 0.11.5)