##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936292_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2594312 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.645534153178183 33.0 31.0 34.0 30.0 34.0 2 31.741722275501175 33.0 31.0 34.0 30.0 34.0 3 31.810963754552265 34.0 31.0 34.0 30.0 34.0 4 35.30227590205033 37.0 35.0 37.0 33.0 37.0 5 35.285338078072336 37.0 35.0 37.0 33.0 37.0 6 35.37049206109366 37.0 35.0 37.0 33.0 37.0 7 35.31770118628754 37.0 35.0 37.0 33.0 37.0 8 35.31329231025413 37.0 35.0 37.0 33.0 37.0 9 36.980469581145215 39.0 37.0 39.0 33.0 39.0 10-11 36.89140107280851 39.0 37.0 39.0 33.0 39.0 12-13 36.795259783711444 39.0 37.0 39.0 33.0 39.0 14-15 38.029697854382974 40.0 38.0 41.0 33.0 41.0 16-17 37.969066365186606 40.0 38.0 41.0 33.0 41.0 18-19 37.90829341266586 40.0 38.0 41.0 33.0 41.0 20-21 37.817754957769154 40.0 38.0 41.0 32.5 41.0 22-23 37.651476961907434 40.0 38.0 41.0 32.0 41.0 24-25 37.81272298782876 40.0 38.0 41.0 33.0 41.0 26-27 37.76707581817453 40.0 38.0 41.0 32.5 41.0 28-29 37.63647433307945 40.0 38.0 41.0 32.0 41.0 30-31 37.48436425534014 40.0 37.5 41.0 32.0 41.0 32-33 37.3087425490843 40.0 37.0 41.0 31.5 41.0 34-35 37.04812277783088 40.0 37.0 41.0 31.0 41.0 36-37 36.8507525309215 40.0 37.0 41.0 30.0 41.0 38-39 36.68371132693369 40.0 36.5 41.0 30.0 41.0 40-41 36.50080252490834 40.0 36.0 41.0 30.0 41.0 42-43 36.313578898760056 40.0 36.0 41.0 30.0 41.0 44-45 36.136399361372106 40.0 35.5 41.0 29.0 41.0 46-47 35.87604998936134 39.0 35.0 41.0 28.0 41.0 48-49 35.70812107410366 39.0 35.0 41.0 27.5 41.0 50-51 35.40371339299205 38.5 35.0 40.5 27.5 41.0 52-53 35.42130399119304 39.0 35.0 40.5 27.5 41.0 54-55 35.50064622142595 39.0 35.0 41.0 27.5 41.0 56-57 35.32694120830494 39.0 35.0 41.0 27.0 41.0 58-59 35.08028698938293 38.5 35.0 41.0 26.5 41.0 60-61 34.77848288871963 38.0 34.5 40.0 26.0 41.0 62-63 34.46333960603042 37.5 34.0 40.0 26.0 41.0 64-65 34.12045178066478 37.0 34.0 40.0 25.5 41.0 66-67 33.75411130195597 36.5 34.0 39.5 25.0 41.0 68-69 33.368790839343916 36.0 33.5 39.0 23.5 41.0 70-71 32.95792757386158 35.5 33.0 39.0 22.5 40.5 72-73 32.54853425493927 35.0 33.0 37.5 22.0 40.0 74-75 32.13557737080197 35.0 32.5 37.0 20.5 39.0 76-77 31.736595868191642 35.0 32.0 36.5 19.5 39.0 78-79 31.34391565085464 35.0 32.0 36.0 18.0 39.0 80-81 30.9578950026057 35.0 31.5 36.0 16.0 37.0 82-83 30.61108571366898 35.0 31.0 35.0 11.5 37.0 84-85 30.29793737222046 34.5 31.0 35.0 7.0 36.5 86-87 29.983896886727578 34.0 31.0 35.0 4.5 36.0 88-89 29.65827683794393 34.0 30.5 35.0 2.0 36.0 90-91 29.306605180872616 34.0 30.0 35.0 2.0 35.5 92-93 28.99118995710616 34.0 30.0 35.0 2.0 35.0 94-95 28.604305303294282 34.0 29.0 35.0 2.0 35.0 96-97 28.176439456780834 34.0 29.0 35.0 2.0 35.0 98-99 27.666972977806836 34.0 29.0 35.0 2.0 35.0 100-101 24.36925493155796 30.0 14.5 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48788949433394535 1101 2 0.3704217647517396 1101 3 0.43159517200900765 1101 4 0.279699913152335 1101 5 0.4919685981790778 1101 6 0.35891264032121484 1101 7 0.43821927309470254 1101 8 0.3820236081113251 1101 9 0.43467906152622504 1101 10-11 0.5831744389255746 1101 12-13 0.46727879279844586 1101 14-15 0.6441785632225105 1101 16-17 0.6644634732747363 1101 18-19 0.6999081462809045 1101 20-21 0.7867242189586321 1101 22-23 0.8069196796026432 1101 24-25 0.7430322858406555 1101 26-27 0.6689984019823001 1101 28-29 0.6389277164574665 1101 30-31 0.6472378481556262 1101 32-33 0.6792389135397343 1101 34-35 0.44757620189708547 1101 36-37 0.4478226689903053 1101 38-39 0.5156207202972851 1101 40-41 0.4820149298481482 1101 42-43 0.5468174273247328 1101 44-45 0.5880798456529703 1101 46-47 0.425194292516359 1101 48-49 0.5419865229444412 1101 50-51 0.630142731736079 1101 52-53 0.6225684419277471 1101 54-55 0.6028572944707165 1101 56-57 0.676824705201831 1101 58-59 0.7337605017741282 1101 60-61 0.5703198378314838 1101 62-63 0.5174649526395214 1101 64-65 0.6227910022423302 1101 66-67 0.7304367838021335 1101 68-69 0.6825409907467375 1101 70-71 0.7764701863193011 1101 72-73 0.7932690087358978 1101 74-75 0.531605027293601 1101 76-77 0.5816187902991885 1101 78-79 0.7193883131496186 1101 80-81 0.738966912626779 1101 82-83 0.9266765714137897 1101 84-85 0.846536207463938 1101 86-87 0.8547681418600703 1101 88-89 0.7228962128414267 1101 90-91 0.7389232066819709 1101 92-93 0.9393484574795004 1101 94-95 1.0822895267328398 1101 96-97 1.1502330100956932 1101 98-99 1.3204432187580757 1101 100-101 1.3081420120732936 1102 1 0.33434975321545224 1102 2 0.3664846673425899 1102 3 0.2968010491492983 1102 4 0.18195669612695298 1102 5 0.27999662250195456 1102 6 0.23161688239564882 1102 7 0.19736956426974217 1102 8 0.28666162727414246 1102 9 0.32952531044205813 1102 10-11 0.45279058271098904 1102 12-13 0.4774969741033388 1102 14-15 0.6017774239305851 1102 16-17 0.5148697498886676 1102 18-19 0.5015732863633104 1102 20-21 0.4843705475496165 1102 22-23 0.5297092627039888 1102 24-25 0.591875153267786 1102 26-27 0.5958301854666459 1102 28-29 0.46658589437458886 1102 30-31 0.6527600183938205 1102 32-33 0.42367341601703146 1102 34-35 0.4922233021450566 1102 36-37 0.4883150551512969 1102 38-39 0.4809550696979201 1102 40-41 0.3916257841759361 1102 42-43 0.38284149612140794 1102 44-45 0.35222640009477857 1102 46-47 0.4961253703737256 1102 48-49 0.5013321986069812 1102 50-51 0.35775791565550463 1102 52-53 0.24808631080566101 1102 54-55 0.3292845069853527 1102 56-57 0.38291652140317467 1102 58-59 0.39166855461067485 1102 60-61 0.3553271074048254 1102 62-63 0.49928435353884737 1102 64-65 0.36723697370670294 1102 66-67 0.5148103183204569 1102 68-69 0.5130279894245646 1102 70-71 0.6003038981618829 1102 72-73 0.605607266610491 1102 74-75 0.5886899009347673 1102 76-77 0.5181109898195757 1102 78-79 0.6592220069555061 1102 80-81 0.5215743211611148 1102 82-83 0.5780324017370866 1102 84-85 0.5070776181532715 1102 86-87 0.5686955308206727 1102 88-89 0.6592569601253366 1102 90-91 0.6782316796951484 1102 92-93 0.5573015981390768 1102 94-95 0.5951601808689695 1102 96-97 0.832691949161454 1102 98-99 0.8693288449216361 1102 100-101 0.8991793025535273 1103 1 0.1565988451745426 1103 2 0.12082825634579919 1103 3 0.18674611077450365 1103 4 0.12805553960956928 1103 5 0.12422562358096911 1103 6 0.16827115056786823 1103 7 0.1729045105471485 1103 8 0.17697237483116623 1103 9 0.1536905966839086 1103 10-11 0.19258433584543866 1103 12-13 0.23790325592275252 1103 14-15 0.2346344473800741 1103 16-17 0.32977900278756067 1103 18-19 0.12422938201535061 1103 20-21 0.24506956808122027 1103 22-23 0.3480052422483695 1103 24-25 0.3408824821837584 1103 26-27 0.3642294119221745 1103 28-29 0.37744087758152745 1103 30-31 0.18878435692970186 1103 32-33 0.2643636220929295 1103 34-35 0.20960221735911233 1103 36-37 0.23526437962964764 1103 38-39 0.28096846235153095 1103 40-41 0.33104625270984656 1103 42-43 0.2793313860926858 1103 44-45 0.182006247665484 1103 46-47 0.17566367526270454 1103 48-49 0.2182259388236929 1103 50-51 0.28488487801169526 1103 52-53 0.3490586554508752 1103 54-55 0.33699031692745507 1103 56-57 0.37685154898634465 1103 58-59 0.40331351441371766 1103 60-61 0.36806342600181097 1103 62-63 0.2746667565418406 1103 64-65 0.334863035620657 1103 66-67 0.3111230643273615 1103 68-69 0.33512719764328835 1103 70-71 0.21653721908405288 1103 72-73 0.20978330496857467 1103 74-75 0.26433987205982845 1103 76-77 0.3026236681983221 1103 78-79 0.20846365981792658 1103 80-81 0.23519037562837042 1103 82-83 0.2295542251136311 1103 84-85 0.3337248195635816 1103 86-87 0.26114929292155153 1103 88-89 0.15439010779786244 1103 90-91 0.33987654451482996 1103 92-93 0.2920428064793761 1103 94-95 0.22135260195679862 1103 96-97 0.35115566931768427 1103 98-99 0.32426807456860374 1103 100-101 0.6740611432550345 1104 1 0.3409359641577119 1104 2 0.3408103088349108 1104 3 0.42530972146062496 1104 4 0.38105659880693565 1104 5 0.4610165497902159 1104 6 0.2768374092473991 1104 7 0.420568391917044 1104 8 0.3725134481511603 1104 9 0.4257807461484262 1104 10-11 0.544540173199799 1104 12-13 0.4850397452719406 1104 14-15 0.5626840531232773 1104 16-17 0.6902922721767553 1104 18-19 0.4775960114139579 1104 20-21 0.596158923147712 1104 22-23 0.7110399898063307 1104 24-25 0.6396548430169915 1104 26-27 0.5271104287516408 1104 28-29 0.5893546412628368 1104 30-31 0.4987099188927502 1104 32-33 0.6485161170911624 1104 34-35 0.6056537649641456 1104 36-37 0.5411046762049097 1104 38-39 0.4990432240321212 1104 40-41 0.4451137176974882 1104 42-43 0.5869524582357926 1104 44-45 0.5620043352258008 1104 46-47 0.627818847676501 1104 48-49 0.4908633402594944 1104 50-51 0.4936960760815978 1104 52-53 0.3930219660310854 1104 54-55 0.569545924789054 1104 56-57 0.6571644535408936 1104 58-59 0.5240693694213618 1104 60-61 0.4599046640858475 1104 62-63 0.47172159942756053 1104 64-65 0.42427400419779815 1104 66-67 0.500597290524837 1104 68-69 0.4933345061051 1104 70-71 0.4519283865549326 1104 72-73 0.4781030060395395 1104 74-75 0.5054786563366278 1104 76-77 0.5990996303025895 1104 78-79 0.5479746637016483 1104 80-81 0.49692392864708523 1104 82-83 0.5573228493339464 1104 84-85 0.36461219157723335 1104 86-87 0.2572449737500797 1104 88-89 0.32987336659508415 1104 90-91 0.5868821053009903 1104 92-93 0.5597983378433895 1104 94-95 0.3188517192923257 1104 96-97 0.5558874864296044 1104 98-99 0.6175840978709424 1104 100-101 0.6300603194348611 1105 1 0.1819117682869198 1105 2 0.19356577543086928 1105 3 0.19406109363395174 1105 4 0.13189415914692404 1105 5 0.11145771242376412 1105 6 0.20599513005682013 1105 7 0.21322730028010284 1105 8 0.2559157822001623 1105 9 0.323693189372527 1105 10-11 0.371031351372757 1105 12-13 0.29020948845812455 1105 14-15 0.23242706109827793 1105 16-17 0.3627466589970467 1105 18-19 0.3220949151009407 1105 20-21 0.3806612412296104 1105 22-23 0.4178596555805498 1105 24-25 0.3335874846324103 1105 26-27 0.2792198333781144 1105 28-29 0.3452895581911051 1105 30-31 0.370043899464207 1105 32-33 0.3314507004209375 1105 34-35 0.06231589811272187 1105 36-37 0.1658692682880769 1105 38-39 0.29576893335662646 1105 40-41 0.27144736324480334 1105 42-43 0.32793763591264025 1105 44-45 0.40020710181234875 1105 46-47 0.27375660874584185 1105 48-49 0.30706945566176813 1105 50-51 0.28670462133552377 1105 52-53 0.3381929495193816 1105 54-55 0.5111786613517282 1105 56-57 0.5817727960695649 1105 58-59 0.5979812181558302 1105 60-61 0.507533869351569 1105 62-63 0.32084722207348904 1105 64-65 0.3629170067553886 1105 66-67 0.28004609028305083 1105 68-69 0.35223606175760125 1105 70-71 0.5508105820892766 1105 72-73 0.46569499511347345 1105 74-75 0.5048364079397558 1105 76-77 0.6195015309586722 1105 78-79 0.5988879965217571 1105 80-81 0.6071490078410875 1105 82-83 0.6077990469383074 1105 84-85 0.5514670919111175 1105 86-87 0.6643930344593052 1105 88-89 0.5314547161578425 1105 90-91 0.6629689378545045 1105 92-93 0.4875803212432963 1105 94-95 0.5272354844503866 1105 96-97 0.5716715783431212 1105 98-99 0.5731789653011781 1105 100-101 0.5148052448884286 1106 1 0.3851258286052719 1106 2 0.4551983481771913 1106 3 0.4198467998636879 1106 4 0.37776547126664184 1106 5 0.3640737621911896 1106 6 0.3135672678969783 1106 7 0.21647454436487834 1106 8 0.21157346011126066 1106 9 0.36519003777173964 1106 10-11 0.3320178005349206 1106 12-13 0.32290681102866614 1106 14-15 0.4228516590450653 1106 16-17 0.4769979725497038 1106 18-19 0.3668362651248813 1106 20-21 0.36371875164496004 1106 22-23 0.47827219355933437 1106 24-25 0.463680704277067 1106 26-27 0.494739826083169 1106 28-29 0.38948481586064787 1106 30-31 0.5022925212923326 1106 32-33 0.4931892521072214 1106 34-35 0.3775058014718553 1106 36-37 0.46809144937339653 1106 38-39 0.4651868873511731 1106 40-41 0.435508264369183 1106 42-43 0.48565514127500364 1106 44-45 0.5653620730867956 1106 46-47 0.45842512588741613 1106 48-49 0.5351330320559029 1106 50-51 0.5951118211158501 1106 52-53 0.5760770181919526 1106 54-55 0.6456845848940063 1106 56-57 0.7281587136744108 1106 58-59 0.6905271603605385 1106 60-61 0.8411993831565994 1106 62-63 0.7208847252144537 1106 64-65 0.6592420417474685 1106 66-67 0.5665844852377475 1106 68-69 0.6556123900081232 1106 70-71 0.6983036822312272 1106 72-73 0.6950989488495409 1106 74-75 0.7108131539355114 1106 76-77 0.7129717678969314 1106 78-79 0.7671289209501815 1106 80-81 0.7747182009647808 1106 82-83 0.7565236830395712 1106 84-85 0.8657878068430271 1106 86-87 0.8336812358134651 1106 88-89 0.7803122244477869 1106 90-91 0.7084013878071751 1106 92-93 0.7796115208398824 1106 94-95 0.9039274537389481 1106 96-97 0.8718144605109259 1106 98-99 0.7717330718094146 1106 100-101 0.7833461601638945 1107 1 -0.017153065070424134 1107 2 0.09647263303854103 1107 3 0.11582836100524574 1107 4 0.005516162084646226 1107 5 -0.07321029810277224 1107 6 0.18016138524429692 1107 7 0.03306814856140505 1107 8 0.15705721996893374 1107 9 0.23452005909180684 1107 10-11 0.1136688064995397 1107 12-13 0.0954832403381829 1107 14-15 0.3142311398192845 1107 16-17 0.007904144637436161 1107 18-19 0.135757710380787 1107 20-21 0.26731815336514586 1107 22-23 0.303749878431816 1107 24-25 0.38710672224669906 1107 26-27 0.29053083722970285 1107 28-29 0.31470758182265257 1107 30-31 0.2670883241869717 1107 32-33 0.2848570516067852 1107 34-35 0.20810050002997826 1107 36-37 0.08878244562956183 1107 38-39 0.02204903686243398 1107 40-41 0.323562347942179 1107 42-43 0.006722502129662189 1107 44-45 0.09660080289993545 1107 46-47 0.166679093398308 1107 48-49 0.12866646779133362 1107 50-51 0.09525325267693319 1107 52-53 0.07682576821963494 1107 54-55 0.2505027467590466 1107 56-57 0.1613478306283227 1107 58-59 0.24467996370334077 1107 60-61 0.154559507162638 1107 62-63 0.34267431430310324 1107 64-65 0.18855888679555477 1107 66-67 0.13001784576507447 1107 68-69 0.12533388236587228 1107 70-71 0.2214364300204963 1107 72-73 0.1734808961685772 1107 74-75 0.1251358768108517 1107 76-77 0.11915456877479613 1107 78-79 0.1766264959075592 1107 80-81 0.20166614388728732 1107 82-83 0.3370323613197712 1107 84-85 0.3202156049957914 1107 86-87 0.06453931576884031 1107 88-89 0.2915181774291291 1107 90-91 0.47192052554099106 1107 92-93 0.205237965426889 1107 94-95 0.2768675359114958 1107 96-97 0.4993832078630227 1107 98-99 0.3015453324403232 1107 100-101 0.28620197937492975 1108 1 -0.03710152467208161 1108 2 -0.005062366954536657 1108 3 -0.032205809164580046 1108 4 -0.006680128833806975 1108 5 0.11040124633741044 1108 6 0.013426431760080959 1108 7 0.03478039437180058 1108 8 0.02442288065081044 1108 9 0.0744373605259625 1108 10-11 -0.028882405153147772 1108 12-13 0.007261055335355593 1108 14-15 -0.08159801209995976 1108 16-17 0.07579159388948398 1108 18-19 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-1.9869467481293555 2316 62-63 -1.9930943881650123 2316 64-65 -1.7993336243499911 2316 66-67 -1.8853857254974713 2316 68-69 -1.966455387158483 2316 70-71 -1.7180563230748334 2316 72-73 -1.718205053327381 2316 74-75 -1.72700476557457 2316 76-77 -2.0580567626019786 2316 78-79 -2.125321221058872 2316 80-81 -1.964264748907155 2316 82-83 -2.055218795484432 2316 84-85 -2.030148558039798 2316 86-87 -2.1693600901774204 2316 88-89 -2.085719192554979 2316 90-91 -2.2227093891633665 2316 92-93 -2.2062828203615936 2316 94-95 -2.1736611630441907 2316 96-97 -2.3926170988786595 2316 98-99 -2.1842082647217573 2316 100-101 -1.97601639871651 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23942.0 3 5285.0 4 4346.0 5 3741.0 6 3680.0 7 3799.0 8 4494.0 9 5180.0 10 6180.0 11 7349.0 12 9405.0 13 13214.0 14 13381.0 15 11630.0 16 13464.0 17 16265.0 18 15067.0 19 12241.0 20 11888.0 21 12449.0 22 13337.0 23 14765.0 24 16812.0 25 19648.0 26 22957.0 27 27660.0 28 33530.0 29 40311.0 30 49318.0 31 61053.0 32 77339.0 33 101107.0 34 140884.0 35 210069.0 36 348927.0 37 576666.0 38 543208.0 39 99644.0 40 77.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.52543663141787 17.484692978379858 12.597166856080252 24.392703534122017 2 15.82037318743644 18.919628462539002 40.26198371767224 24.998014632352316 3 20.39381315964167 22.753507873102077 27.720350868265577 29.13232809899068 4 12.86265466899034 14.789475936224663 35.83795041880464 36.50991897598036 5 14.534622167824754 36.9606892630321 33.4990314466621 15.00565712248104 6 34.92591234840451 34.363186191121045 16.58993653815093 14.120964922323514 7 31.070102650074993 30.906850906635036 19.39415292775715 18.628893515532816 8 27.360291498525086 35.24832269614548 18.720037131212884 18.671348674116555 9 27.023699054050248 15.815753955143238 18.928247454316878 38.232299536489634 10-11 26.36403094392541 25.632564592109986 26.442473925899712 21.56093053806489 12-13 26.570724324591044 22.503467540047293 27.184820077268895 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23.52222913060661 25.9732705550307 27.101490836235726 23.40300947812696 40-41 23.739882084671123 25.867718848518585 27.1231789495457 23.26922011726459 42-43 23.677308847892622 25.77201080194466 26.892117078517437 23.658563271645285 44-45 23.59242080862966 26.14398402798666 26.94880197310456 23.314793190279122 46-47 23.74450441641735 26.2349059092559 26.65512508566118 23.365464588665567 48-49 23.900303309831244 26.206837236637842 26.55233692689687 23.340522526634043 50-51 23.731227278134043 26.409593562652972 26.53584606222774 23.323333096985245 52-53 24.016006409382246 26.2871259127481 26.41630921240222 23.280558465467436 54-55 23.747594218415625 26.26616693940423 26.520123732273916 23.466115109906234 56-57 23.676362275034506 26.51861572687625 26.375086352934296 23.42993564515495 58-59 23.656422099014378 26.202877454849972 26.52032012692284 23.620380319212806 60-61 23.60719049839724 26.281627208346436 26.646915558006906 23.464266735249414 62-63 23.603700780920935 26.36212848158566 26.756510498617775 23.277660238875626 64-65 23.48418407033572 26.313061506565305 26.624921361964294 23.577833061134683 66-67 23.408296558494747 26.5872304334945 26.50199155356669 23.50248145444406 68-69 23.653834420737937 26.1930831576409 26.731404122291618 23.421678299329542 70-71 23.577925207803975 26.081913971294075 26.56579421562968 23.774366605272274 72-73 23.519755635683044 26.49519564290304 26.40244736162224 23.582601359791674 74-75 23.559332112660616 26.548895573986382 26.32573831620382 23.56603399714918 76-77 23.712282999972306 26.39866088781062 26.349507429279928 23.539548682937145 78-79 23.6075656042767 26.502003226155686 26.377802623493224 23.512628546074396 80-81 23.636508013363677 26.492538614244904 26.268338740134606 23.602614632256817 82-83 23.802275698445747 26.64269597637147 26.193076066431026 23.361952258751757 84-85 23.731612000574827 26.656454823536794 26.03712054687654 23.574812629011834 86-87 23.621458731547385 26.42546023682797 26.419630470272352 23.53345056135229 88-89 23.671003305215528 26.53030626755103 26.250264651627415 23.548425775606024 90-91 23.5789428407348 26.70529184139899 26.254720775753366 23.461044542112848 92-93 23.6311308421861 26.591424938181103 26.304822377704745 23.472621841928053 94-95 23.62579824737578 26.413778825075152 26.439556698053956 23.520866229495116 96-97 23.683554244767908 26.274681179646926 26.362547495829354 23.67921707975582 98-99 23.405202748029826 26.342926785353004 26.268001637898575 23.983868828718595 100-101 23.61369594597211 26.4471202809822 26.137850114429767 23.801333658615924 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 126.0 1 101.0 2 138.0 3 227.0 4 190.0 5 180.5 6 379.5 7 694.5 8 1041.5 9 1254.5 10 1241.5 11 1233.5 12 1392.5 13 1647.0 14 1899.0 15 2084.5 16 2302.0 17 2520.0 18 2613.5 19 2741.5 20 2948.0 21 3259.0 22 3645.0 23 4333.0 24 5404.5 25 6664.0 26 8820.5 27 11862.5 28 14573.5 29 17513.5 30 21273.0 31 25884.5 32 31586.0 33 35601.5 34 38634.5 35 43494.5 36 50259.0 37 55733.5 38 59563.0 39 63983.5 40 68394.0 41 72217.5 42 74751.0 43 78718.0 44 83469.0 45 89028.5 46 93455.5 47 96184.0 48 99592.0 49 100818.0 50 99648.5 51 97881.0 52 98085.5 53 100419.5 54 101495.0 55 96530.5 56 88959.0 57 82521.5 58 78019.0 59 71581.0 60 61795.0 61 52649.5 62 43626.0 63 34214.0 64 24683.0 65 17749.5 66 13211.5 67 10566.5 68 8521.5 69 6738.5 70 5418.0 71 3786.0 72 2687.0 73 2231.5 74 1795.5 75 1289.0 76 892.0 77 505.0 78 297.0 79 195.5 80 129.5 81 86.5 82 61.0 83 52.0 84 36.5 85 27.5 86 30.5 87 27.5 88 23.0 89 20.5 90 21.5 91 21.5 92 21.0 93 23.0 94 18.5 95 20.5 96 25.0 97 22.5 98 17.5 99 13.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04706450110857908 2 0.012874318894566267 3 0.011178300836599453 4 0.005512058688392144 5 0.009135369994048518 6 0.005974609067837639 7 0.006128792527652803 8 0.010330291807616045 9 0.01584235049600819 10-11 0.0027367564117191765 12-13 0.009790649698262969 14-15 0.009809922630739865 16-17 0.020159487370832806 18-19 0.012488860245028353 20-21 0.004105134617578764 22-23 0.01031101887513915 24-25 0.02241442047062959 26-27 0.007882629383050304 28-29 0.019118749017080446 30-31 0.015341254251608902 32-33 0.01298995648942764 34-35 0.2662748351007897 36-37 0.4691802682175467 38-39 0.466539876468212 40-41 0.48679572850142927 42-43 0.47679307654592046 44-45 0.4932521608811893 46-47 0.49805112106793625 48-49 0.5058373857886022 50-51 0.5154738520270499 52-53 0.5047966474348498 54-55 0.49224996839239077 56-57 0.48608262999978413 58-59 0.4851382563084162 60-61 0.48244004576165084 62-63 0.4789130991183789 64-65 0.49585400676557023 66-67 0.46860208024323985 68-69 0.4956805503732782 70-71 0.47687016827582807 72-73 0.48540807736309277 74-75 0.49911113235416554 76-77 0.48573571721520004 78-79 0.48573571721520004 80-81 0.4890313886687492 82-83 0.4890313886687492 84-85 0.48833756309958093 86-87 0.4908237713891005 88-89 0.5045075534476963 90-91 0.528001258137032 92-93 0.5159749482714493 94-95 0.48723900594839786 96-97 0.4617794621464188 98-99 0.45231645230026307 100-101 0.46031471927817474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2594312.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.03950998340925 #Duplication Level Percentage of deduplicated Percentage of total 1 68.96774497344964 31.752411832383583 2 14.82925746820147 13.654634945076157 3 6.143278073980174 8.48500536653608 4 3.124496743561996 5.754011960734089 5 1.8003902417824595 4.144454225528807 6 1.1705589367284803 3.2335175912207874 7 0.7755249794618482 2.499335302402191 8 0.561246764430681 2.0671620811329983 9 0.4166836050354732 1.7265518094558265 >10 2.0253472166972695 16.350728411116723 >50 0.12235993804549487 3.8365488794789786 >100 0.05908564942161932 4.696181224198445 >500 0.0030190569027199947 0.9526624574427843 >1k 0.0010063523009033023 0.8467939132926069 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4319 0.16647959073542426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2955 0.11390303093845304 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1563759486137365E-4 2 0.0 0.0 0.0 0.0 1.1563759486137365E-4 3 0.0 0.0 0.0 0.0 1.1563759486137365E-4 4 0.0 0.0 0.0 0.0 1.1563759486137365E-4 5 0.0 0.0 0.0 0.0 1.1563759486137365E-4 6 0.0 0.0 0.0 0.0 1.5418345981516488E-4 7 0.0 0.0 0.0 0.0 1.5418345981516488E-4 8 0.0 0.0 0.0 3.854586495379122E-5 1.5418345981516488E-4 9 0.0 0.0 0.0 3.854586495379122E-5 1.5418345981516488E-4 10-11 0.0 0.0 0.0 5.7818797430686826E-5 1.5418345981516488E-4 12-13 0.0 0.0 0.0 7.709172990758244E-5 5.396421093530771E-4 14-15 0.0 0.0 0.0 9.636466238447804E-5 6.552797042144508E-4 16-17 0.0 0.0 0.0 2.120022572458517E-4 8.287360965065112E-4 18-19 0.0 0.0 0.0 2.6982105467653853E-4 9.251007588909892E-4 20-21 0.0 0.0 0.0 5.589150418299726E-4 9.443736913678848E-4 22-23 3.854586495379122E-5 0.0 0.0 0.0011178300836599453 9.636466238447804E-4 24-25 3.854586495379122E-5 0.0 0.0 0.001830928585305083 0.0010021924887985716 26-27 3.854586495379122E-5 0.0 0.0 0.002293478964750577 0.0010600112862292586 28-29 3.854586495379122E-5 0.0 0.0 0.002756029344196072 0.0010792842187061541 30-31 3.854586495379122E-5 0.0 0.0 0.004548412064547364 0.0010985571511830496 32-33 3.854586495379122E-5 0.0 0.0 0.007227349678835853 0.0011178300836599453 34-35 3.854586495379122E-5 0.0 0.0 0.011467394823752888 0.001233467678521319 36-37 3.854586495379122E-5 0.0 0.0 0.018463469312865993 0.001233467678521319 38-39 3.854586495379122E-5 0.0 0.0 0.030528325043402645 0.001233467678521319 40-41 3.854586495379122E-5 0.0 0.0 0.04357610033026097 0.001233467678521319 42-43 3.854586495379122E-5 0.0 0.0 0.05683587787436515 0.001329832340905797 44-45 3.854586495379122E-5 0.0 0.0 0.07254331784303508 0.0014261970032902749 46-47 3.854586495379122E-5 0.0 0.0 0.09089114956103969 0.0015418345981516486 48-49 3.854586495379122E-5 0.0 0.0 0.11421139785808337 0.0016381992605361269 50-51 3.854586495379122E-5 0.0 0.0 0.13918911834814007 0.001773109787874396 52-53 3.854586495379122E-5 0.0 0.0 0.1635886508638899 0.0018502015177819784 54-55 3.854586495379122E-5 0.0 0.0 0.19074421272383585 0.0019465661801664565 56-57 3.854586495379122E-5 0.0 0.0 0.2233154686097894 0.0020429308425509345 58-59 3.854586495379122E-5 0.0 0.0 0.2615336937114734 0.002120022572458517 60-61 3.854586495379122E-5 0.0 0.0 0.30532179629898026 0.0021392955049354123 62-63 3.854586495379122E-5 0.0 0.0 0.34722115150375127 0.0022356601673198906 64-65 3.854586495379122E-5 0.0 0.0 0.3963285834548813 0.0022934789647505774 66-67 3.854586495379122E-5 0.0 0.0 0.4515455350011872 0.002351297762181264 68-69 3.854586495379122E-5 0.0 0.0 0.515281122702281 0.0023898436271350553 70-71 3.854586495379122E-5 0.0 0.0 0.5849527736062585 0.0024091165596119507 72-73 3.854586495379122E-5 0.0 0.0 0.6601364831986284 0.0025825729519040113 74-75 3.854586495379122E-5 0.0 0.0 0.734433637897061 0.0027367564117191765 76-77 3.854586495379122E-5 0.0 0.0 0.8188298092133868 0.0027367564117191765 78-79 3.854586495379122E-5 0.0 0.0 0.9189141475659057 0.002775302276672968 80-81 3.854586495379122E-5 0.0 0.0 1.0308127935267617 0.0028331210741036547 82-83 3.854586495379122E-5 0.0 0.0 1.1510566192501133 0.003006577466395715 84-85 3.854586495379122E-5 0.0 0.0 1.2691611494685295 0.003006577466395715 86-87 3.854586495379122E-5 0.0 0.0 1.39686360006044 0.0031222150612570886 88-89 3.854586495379122E-5 0.0 0.0 1.54840666812627 0.003257125588595358 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3590 0.0 43.24893 1 GTATCAA 6025 0.0 39.597656 1 ATCAACG 7155 0.0 32.875122 3 TCAACGC 7210 0.0 32.689358 4 TATACCG 195 0.0 31.551575 5 CAACGCA 7470 0.0 31.551573 5 TATCAAC 7700 0.0 30.669994 2 AACGCAG 7845 0.0 30.221443 6 TACATGG 9285 0.0 27.575237 2 GTACATG 9605 0.0 27.204311 1 ACGCAGA 9120 0.0 25.945015 7 ACATGGG 9620 0.0 25.533653 3 CGCAGAG 9435 0.0 24.929777 8 ATACCGT 245 3.1286618E-10 23.178513 6 CATGGGG 6110 0.0 23.005281 4 ATACCGA 195 4.3804903E-7 21.841291 6 GCAGAGT 11175 0.0 20.581446 9 GAGTACA 7405 0.0 19.17751 1 GTGTAGC 1705 0.0 19.156702 1 CAGAGTA 9965 0.0 18.688908 10-11 >>END_MODULE