FastQCFastQC Report
Thu 26 May 2016
SRR936292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594312
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43400.1672890538994539No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32620.12573661147926696No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36950.042.6234971
GTATCAA63550.038.4842571
ATCAACG76500.031.6236023
CAACGCA77400.031.4397475
TCAACGC77450.031.3582024
TATCAAC79650.030.498532
AACGCAG81250.029.8916056
TACATGG95300.026.1373212
GTACATG98950.026.1107621
ACGCAGA96000.025.1012367
ACATGGG98550.024.8368023
CGCAGAG97950.024.3109478
CATGGGG61850.023.008284
GCAGAGT117650.019.9982689
AGTACAT71400.019.4699152
GTACCTG20200.019.3151451
GAGTACT59400.019.08633212-13
GAGTACA78750.019.0324861
CAGAGTA102100.018.58425910-11
AGAGTAC102150.018.1804410-11
Produced by FastQC (version 0.11.5)