FastQCFastQC Report
Thu 26 May 2016
SRR936291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2613330
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47830.18302319263162325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31970.12233433971216799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61000.038.282891
TACATGG101350.032.8026962
GTACATG106300.032.0838781
ATCAACG71950.031.99033
GGTATCA40750.031.7338431
ACATGGG101150.031.5109863
TCAACGC73350.031.3797134
CAACGCA74800.030.7714165
TATCAAC76700.030.193242
AACGCAG77500.029.7587816
CATGGGG60600.026.5716864
ACGCAGA93550.024.4000727
CGCAGAG95650.023.8643688
CATGGGA45550.023.6020814
GTACCTG24050.023.4379441
AGTACAT68600.022.3674832
GAGTACA77150.022.2873461
TACCTGG24950.022.0182912
TAAGGTG8200.021.9473745
ATGGGGA30650.019.9329725
Produced by FastQC (version 0.11.5)