##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936291_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2613330 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3904195030861 34.0 31.0 34.0 31.0 34.0 2 32.50489758277753 34.0 31.0 34.0 31.0 34.0 3 32.57803989545905 34.0 31.0 34.0 31.0 34.0 4 36.053512568255826 37.0 37.0 37.0 35.0 37.0 5 36.05380989006363 37.0 37.0 37.0 35.0 37.0 6 36.1424913807288 37.0 37.0 37.0 35.0 37.0 7 36.101615180631605 37.0 37.0 37.0 35.0 37.0 8 36.109972716801934 37.0 37.0 37.0 35.0 37.0 9 37.87481221277068 39.0 38.0 39.0 35.0 39.0 10-11 37.818090520523626 39.0 38.0 39.0 35.0 39.0 12-13 37.7467476361577 39.0 38.0 39.0 35.0 39.0 14-15 39.13187025748757 40.5 39.0 41.0 36.0 41.0 16-17 39.08559481580971 40.0 39.0 41.0 36.0 41.0 18-19 39.030391301519515 40.0 39.0 41.0 36.0 41.0 20-21 38.94922340462169 40.0 39.0 41.0 36.0 41.0 22-23 38.79963762708881 40.0 38.5 41.0 35.5 41.0 24-25 38.96785251001596 40.0 39.0 41.0 36.0 41.0 26-27 38.94130974656856 40.0 39.0 41.0 36.0 41.0 28-29 38.83985030593151 40.0 39.0 41.0 35.0 41.0 30-31 38.7142699161606 40.0 38.0 41.0 35.0 41.0 32-33 38.569738418033694 40.0 38.0 41.0 35.0 41.0 34-35 38.338818289309046 40.0 38.0 41.0 34.5 41.0 36-37 38.164448232714584 40.0 38.0 41.0 34.0 41.0 38-39 38.01490378176503 40.0 38.0 41.0 34.0 41.0 40-41 37.85344828245954 40.0 38.0 41.0 33.0 41.0 42-43 37.68092912108307 40.0 38.0 41.0 33.0 41.0 44-45 37.51164280821787 40.0 37.0 41.0 33.0 41.0 46-47 37.259825969165774 40.0 37.0 41.0 32.5 41.0 48-49 37.11098674870759 40.0 37.0 41.0 32.0 41.0 50-51 36.8155372647159 39.5 36.5 40.5 31.5 41.0 52-53 36.84010821442374 39.0 36.0 40.5 32.0 41.0 54-55 36.90554063206713 40.0 36.0 41.0 32.0 41.0 56-57 36.72890316186628 39.0 36.0 41.0 32.0 41.0 58-59 36.48817964053526 39.0 35.0 41.0 31.5 41.0 60-61 36.18394940554771 39.0 35.0 41.0 31.0 41.0 62-63 35.87098510329733 38.0 35.0 40.0 31.0 41.0 64-65 35.52602943371102 37.5 35.0 40.0 30.5 41.0 66-67 35.162105245032194 37.0 35.0 40.0 30.0 41.0 68-69 34.775893591701006 36.0 34.5 39.0 29.5 41.0 70-71 34.37598141069057 36.0 34.0 39.0 29.0 40.5 72-73 33.97737369562971 35.0 34.0 38.5 29.0 40.0 74-75 33.57938052216903 35.0 34.0 37.0 28.5 39.5 76-77 33.20192455602622 35.0 34.0 37.0 28.0 39.0 78-79 32.836139140483596 35.0 33.0 36.0 27.0 39.0 80-81 32.480320701939675 35.0 33.0 36.0 26.0 37.0 82-83 32.16366551487948 35.0 33.0 35.0 26.0 37.0 84-85 31.889275751627235 35.0 33.0 35.0 25.5 36.5 86-87 31.61192501521048 35.0 32.5 35.0 25.0 36.0 88-89 31.329906096819002 35.0 32.0 35.0 24.5 36.0 90-91 31.024756919332805 34.5 32.0 35.0 23.5 35.5 92-93 30.753347261922528 34.0 32.0 35.0 21.0 35.0 94-95 30.423533575935686 34.0 31.0 35.0 19.0 35.0 96-97 30.05547271106213 34.0 31.0 35.0 12.0 35.0 98-99 29.617490328431543 34.0 31.0 35.0 2.0 35.0 100-101 26.221060103392986 30.5 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21269722034384841 1101 2 0.1263429064671726 1101 3 0.22097651095463533 1101 4 0.051903021779466485 1101 5 0.2260646489085758 1101 6 0.17393943915409693 1101 7 0.16523184049238182 1101 8 0.16582461645055702 1101 9 0.2431653913834637 1101 10-11 0.3044399380687892 1101 12-13 0.22488329218418102 1101 14-15 0.38725952909779693 1101 16-17 0.40764420008934366 1101 18-19 0.39024294924884373 1101 20-21 0.4516076177604802 1101 22-23 0.4955716374870818 1101 24-25 0.2838250458885838 1101 26-27 0.29101583794485464 1101 28-29 0.33736168938580846 1101 30-31 0.314630646736596 1101 32-33 0.35072569960531297 1101 34-35 0.20770328367068203 1101 36-37 0.12235004749093292 1101 38-39 0.17967810830536024 1101 40-41 0.2317125689526378 1101 42-43 0.2493610298835094 1101 44-45 0.20952319604239733 1101 46-47 0.2308138491957621 1101 48-49 0.2865783710822356 1101 50-51 0.3074085290801989 1101 52-53 0.32133187100364324 1101 54-55 0.32001124901255906 1101 56-57 0.3615346763823837 1101 58-59 0.4266797580243562 1101 60-61 0.21662432318913716 1101 62-63 0.17556022743444544 1101 64-65 0.2732654614273571 1101 66-67 0.29550014494191856 1101 68-69 0.23029356888028474 1101 70-71 0.362188895913647 1101 72-73 0.3948826050016123 1101 74-75 0.3986509893126069 1101 76-77 0.3451421494908615 1101 78-79 0.3740879728739017 1101 80-81 0.31670446723954626 1101 82-83 0.2624114725307152 1101 84-85 0.30763913340282656 1101 86-87 0.2398553733567148 1101 88-89 0.2809758477284099 1101 90-91 0.42130767449562256 1101 92-93 0.5396036286445494 1101 94-95 0.5996986902641055 1101 96-97 0.6196037459088259 1101 98-99 0.6943869463551522 1101 100-101 0.9125488480173161 1102 1 0.25247531773443654 1102 2 0.23963925603226954 1102 3 0.21321813824043545 1102 4 0.19407410595177765 1102 5 0.19908841440409475 1102 6 0.13458049113683757 1102 7 0.15157250209090023 1102 8 0.10706699710941336 1102 9 0.18636928152797338 1102 10-11 0.2505723947498453 1102 12-13 0.2892953187577092 1102 14-15 0.4208599674239366 1102 16-17 0.3308165855423866 1102 18-19 0.29516726950087957 1102 20-21 0.31208429743994515 1102 22-23 0.3664900677315899 1102 24-25 0.4255783504567674 1102 26-27 0.35587714881397403 1102 28-29 0.3662076588399543 1102 30-31 0.29168153366214966 1102 32-33 0.17692527497686683 1102 34-35 0.059029905134970306 1102 36-37 0.09260140013801532 1102 38-39 0.2171728004497382 1102 40-41 0.29762038348588504 1102 42-43 0.23715501659675198 1102 44-45 0.21958141123313624 1102 46-47 0.3808761736940838 1102 48-49 0.38833578495800225 1102 50-51 0.23624491014998483 1102 52-53 0.3008127284791584 1102 54-55 0.3617001744286199 1102 56-57 0.39269144646662113 1102 58-59 0.27055757461226193 1102 60-61 0.3700302542897447 1102 62-63 0.44032976376757915 1102 64-65 0.45296548197389086 1102 66-67 0.41802599933544826 1102 68-69 0.32333377160612287 1102 70-71 0.43912883153449656 1102 72-73 0.4191631337325248 1102 74-75 0.4935718851416553 1102 76-77 0.3691473546138013 1102 78-79 0.3161371475879591 1102 80-81 0.3890940295982972 1102 82-83 0.4295397514157173 1102 84-85 0.424109238190173 1102 86-87 0.4364419085255413 1102 88-89 0.36077586142609874 1102 90-91 0.30454717863917224 1102 92-93 0.3880140114950379 1102 94-95 0.33088833480899993 1102 96-97 0.42205152345830754 1102 98-99 0.49447735112194735 1102 100-101 0.5454959064379707 1103 1 0.1892263276830306 1103 2 0.16008972222348206 1103 3 0.15758016311455236 1103 4 0.11898317086910737 1103 5 0.1599680624178177 1103 6 0.09116143792062559 1103 7 0.1643785972563876 1103 8 0.1443739420612573 1103 9 0.1953108342279677 1103 10-11 0.22114119567745405 1103 12-13 0.2383346101258894 1103 14-15 0.2890252760208156 1103 16-17 0.3383140308886894 1103 18-19 0.2539841339215698 1103 20-21 0.2950785104212983 1103 22-23 0.3680715704503754 1103 24-25 0.3795392549894885 1103 26-27 0.4360368874715803 1103 28-29 0.4339814303559635 1103 30-31 0.38598478826450133 1103 32-33 0.39184789411903154 1103 34-35 0.22790707526696252 1103 36-37 0.22045612637973733 1103 38-39 0.25314444246995293 1103 40-41 0.2740961261885815 1103 42-43 0.3007899843789943 1103 44-45 0.35815377014476724 1103 46-47 0.4193929391053999 1103 48-49 0.4418670311524977 1103 50-51 0.37347508312519295 1103 52-53 0.43568308973101466 1103 54-55 0.4772136730599641 1103 56-57 0.4405913358090814 1103 58-59 0.5600742288365765 1103 60-61 0.5017995571472511 1103 62-63 0.5118807165342787 1103 64-65 0.5259764259515265 1103 66-67 0.6157331645434496 1103 68-69 0.5447558003982635 1103 70-71 0.4872606251926044 1103 72-73 0.4113363630795419 1103 74-75 0.3635738970969058 1103 76-77 0.34438865687830855 1103 78-79 0.37852258526764615 1103 80-81 0.41241894448729255 1103 82-83 0.3543836925183754 1103 84-85 0.3156296410047936 1103 86-87 0.42281276482428254 1103 88-89 0.37324866032058424 1103 90-91 0.4599312767802317 1103 92-93 0.5067762883930236 1103 94-95 0.38204256176814155 1103 96-97 0.5069745661700011 1103 98-99 0.6137862974398054 1103 100-101 0.9851772233156311 1104 1 0.3228173193270152 1104 2 0.2792802186050025 1104 3 0.3119030836846832 1104 4 0.2610225762666829 1104 5 0.30611104173362946 1104 6 0.2663259692983715 1104 7 0.2898449163632435 1104 8 0.2695584420436319 1104 9 0.3197332076891257 1104 10-11 0.2769081868724541 1104 12-13 0.3133530815129433 1104 14-15 0.4790588889830545 1104 16-17 0.5053165020676786 1104 18-19 0.41546683447086963 1104 20-21 0.5352352887786864 1104 22-23 0.5663187478958136 1104 24-25 0.5073356724130704 1104 26-27 0.40026288416503775 1104 28-29 0.4617744084568898 1104 30-31 0.4393226081172301 1104 32-33 0.534598349529837 1104 34-35 0.46166123795958214 1104 36-37 0.48900245987356783 1104 38-39 0.5022415774970739 1104 40-41 0.4702214468925803 1104 42-43 0.5005275349721146 1104 44-45 0.41223292658330024 1104 46-47 0.5073235859341949 1104 48-49 0.4982488226597326 1104 50-51 0.4042879918441997 1104 52-53 0.4419775635095178 1104 54-55 0.5384612505619302 1104 56-57 0.4648407849185716 1104 58-59 0.4854907205426713 1104 60-61 0.411508097652721 1104 62-63 0.4061812626850241 1104 64-65 0.48137756561642675 1104 66-67 0.5537961825165638 1104 68-69 0.49127073989184566 1104 70-71 0.46241023572780904 1104 72-73 0.4510893718024107 1104 74-75 0.3678293316544696 1104 76-77 0.4405223448896507 1104 78-79 0.4905238706503212 1104 80-81 0.4904337332324573 1104 82-83 0.5318647189217671 1104 84-85 0.41322247448306015 1104 86-87 0.45855956154867883 1104 88-89 0.42337490570616154 1104 90-91 0.5348663317597726 1104 92-93 0.636086007781941 1104 94-95 0.5104159640658814 1104 96-97 0.5912451577026978 1104 98-99 0.5503241212622996 1104 100-101 0.8678719377440487 1105 1 0.2421884346108314 1105 2 0.1912445587094993 1105 3 0.21804181386572452 1105 4 0.12737377971284758 1105 5 0.14582861429016702 1105 6 0.1745059222382821 1105 7 0.25760792231771745 1105 8 0.2610750754067581 1105 9 0.26637808480072067 1105 10-11 0.26953697452302805 1105 12-13 0.26659323186422057 1105 14-15 0.31391832794384555 1105 16-17 0.31509109230020016 1105 18-19 0.31033867439964524 1105 20-21 0.3185391677211342 1105 22-23 0.35616434663997865 1105 24-25 0.34967083660456666 1105 26-27 0.28628727775602414 1105 28-29 0.27026622649569276 1105 30-31 0.4155642947827545 1105 32-33 0.293774480093127 1105 34-35 0.2112333125193615 1105 36-37 0.28220318150614787 1105 38-39 0.3364260757850346 1105 40-41 0.38840283908114515 1105 42-43 0.36131093809227366 1105 44-45 0.3226703242878699 1105 46-47 0.3029827450782747 1105 48-49 0.32860040097995835 1105 50-51 0.34077989021316313 1105 52-53 0.3457417844576156 1105 54-55 0.3503154714096155 1105 56-57 0.43986145319853875 1105 58-59 0.39405583984969184 1105 60-61 0.32545332922113346 1105 62-63 0.22318021169880353 1105 64-65 0.2925219523793601 1105 66-67 0.1719097961352034 1105 68-69 0.2597624909888623 1105 70-71 0.31884376084939703 1105 72-73 0.30761142745112835 1105 74-75 0.42046976717779927 1105 76-77 0.528127987413761 1105 78-79 0.37067989524474143 1105 80-81 0.4318054376040976 1105 82-83 0.3545423406135839 1105 84-85 0.35196512343692277 1105 86-87 0.38937805890614996 1105 88-89 0.48145578478584383 1105 90-91 0.5700396440733826 1105 92-93 0.5814853490746543 1105 94-95 0.5631264374448044 1105 96-97 0.5664290802912006 1105 98-99 0.5964024197356892 1105 100-101 0.825518950298985 1106 1 0.2276966380683163 1106 2 0.20438232005101042 1106 3 0.22261412854194162 1106 4 0.2589436387327382 1106 5 0.2329789767685213 1106 6 0.22196759541097322 1106 7 0.1990307354628129 1106 8 0.16787215601294037 1106 9 0.2559904628739602 1106 10-11 0.2660904951123868 1106 12-13 0.2456878675759242 1106 14-15 0.3048778927741509 1106 16-17 0.3147430445958932 1106 18-19 0.3149288439475484 1106 20-21 0.23875822009216563 1106 22-23 0.30791510241468956 1106 24-25 0.31452804654849587 1106 26-27 0.3342934022216042 1106 28-29 0.3290667215403502 1106 30-31 0.40434067352127556 1106 32-33 0.42566352551786935 1106 34-35 0.27204162457359615 1106 36-37 0.41688467994568157 1106 38-39 0.35468011392108423 1106 40-41 0.33395575309403824 1106 42-43 0.31728204744235455 1106 44-45 0.33280748883640854 1106 46-47 0.48910584096626764 1106 48-49 0.3777443454879972 1106 50-51 0.45385626014653724 1106 52-53 0.3912049349374911 1106 54-55 0.4154759695849961 1106 56-57 0.36442095150109566 1106 58-59 0.37905065459928267 1106 60-61 0.49248369717824403 1106 62-63 0.388348292079975 1106 64-65 0.3244423049763867 1106 66-67 0.3063589366554922 1106 68-69 0.3223570239739004 1106 70-71 0.41344596409164325 1106 72-73 0.5233670064870637 1106 74-75 0.44506766568386524 1106 76-77 0.4791095161821133 1106 78-79 0.5128543251117819 1106 80-81 0.6053821842018863 1106 82-83 0.4873965274586993 1106 84-85 0.6061580832365614 1106 86-87 0.5660419861952484 1106 88-89 0.393572149631801 1106 90-91 0.4483684770006988 1106 92-93 0.5574681007794275 1106 94-95 0.47829035984215196 1106 96-97 0.4532905851878404 1106 98-99 0.4331323366738502 1106 100-101 0.6297593600315992 1107 1 -0.004716513359390717 1107 2 0.055491395107040375 1107 3 0.08521575373328716 1107 4 0.048111656568849526 1107 5 0.030296457282254607 1107 6 0.15194049085041428 1107 7 0.0447444336491003 1107 8 0.14848921598998288 1107 9 0.10240545013339641 1107 10-11 0.10311434739625014 1107 12-13 0.148821532224801 1107 14-15 0.19676015467577912 1107 16-17 0.03985802082952006 1107 18-19 0.08766493332833392 1107 20-21 0.23515682082852862 1107 22-23 0.20717375175932773 1107 24-25 0.23997266415909024 1107 26-27 0.22045898287948518 1107 28-29 0.2228073910304431 1107 30-31 0.21360306690740316 1107 32-33 0.29421080359632157 1107 34-35 0.2690240691790322 1107 36-37 0.15662872888135126 1107 38-39 0.19729474686635484 1107 40-41 0.28404543083589573 1107 42-43 0.009794945276361489 1107 44-45 0.23052928862793465 1107 46-47 0.31358715424209294 1107 48-49 0.1808169792165657 1107 50-51 0.2197575290620577 1107 52-53 0.28535433741282645 1107 54-55 0.25383416511257195 1107 56-57 0.28957833991209014 1107 58-59 0.41861506056837783 1107 60-61 0.4069944114953614 1107 62-63 0.4801206002426568 1107 64-65 0.2939807055840902 1107 66-67 0.3135689222542837 1107 68-69 0.3222434682438262 1107 70-71 0.34691482022778075 1107 72-73 0.23888893331218952 1107 74-75 0.18999190211247452 1107 76-77 0.31896896412032305 1107 78-79 0.3994141800580735 1107 80-81 0.37618224562174873 1107 82-83 0.3781308872073126 1107 84-85 0.4264276880457025 1107 86-87 0.22666229641969338 1107 88-89 0.49329547274427554 1107 90-91 0.4985963832664062 1107 92-93 0.15420878920956227 1107 94-95 0.3415090585391418 1107 96-97 0.31691167533637454 1107 98-99 0.2465230684969839 1107 100-101 0.1388592072260053 1108 1 -0.1144559352999508 1108 2 -0.03601623415553945 1108 3 -0.004495170561384043 1108 4 -0.052775298170608664 1108 5 0.023960690897261827 1108 6 0.00441524665291837 1108 7 -0.011584073653665428 1108 8 0.017576923440429937 1108 9 0.023730630083086623 1108 10-11 0.016702610281448926 1108 12-13 -0.04486014289547313 1108 14-15 -0.13164417423357122 1108 16-17 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-0.9445107238565669 2316 58-59 -1.0330134051418227 2316 60-61 -1.1273721484105863 2316 62-63 -1.0575631884143206 2316 64-65 -0.9565238394804254 2316 66-67 -1.003458083711827 2316 68-69 -0.9904534247217001 2316 70-71 -0.9084103738414839 2316 72-73 -0.905118734897222 2316 74-75 -0.8056661647422629 2316 76-77 -1.0404561711692608 2316 78-79 -1.1744114520861828 2316 80-81 -0.9101991382049057 2316 82-83 -0.9463675446534907 2316 84-85 -0.9965500603130444 2316 86-87 -1.0183750669117124 2316 88-89 -0.967737961045696 2316 90-91 -1.117478823790428 2316 92-93 -1.1803266966158965 2316 94-95 -1.157521689597214 2316 96-97 -1.3384534007766007 2316 98-99 -1.1811746387451763 2316 100-101 -1.2987013892564363 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9261.0 3 1912.0 4 1757.0 5 1473.0 6 1418.0 7 1458.0 8 1708.0 9 1961.0 10 2425.0 11 3052.0 12 4729.0 13 8690.0 14 9990.0 15 7024.0 16 8809.0 17 11884.0 18 10895.0 19 7334.0 20 7007.0 21 7188.0 22 7658.0 23 8318.0 24 9479.0 25 10807.0 26 12981.0 27 16114.0 28 20231.0 29 25606.0 30 32942.0 31 44527.0 32 60505.0 33 87451.0 34 135044.0 35 223569.0 36 403639.0 37 678939.0 38 617467.0 39 108002.0 40 76.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.933808906761705 17.225034787551895 11.927220804774365 24.91393550091204 2 16.369023815382473 18.89927187298973 38.728611484331324 26.003092827296477 3 20.470471811183682 22.52235221363833 27.584912389731375 29.422263585446608 4 13.114892109153262 14.165081471566173 34.679591603230165 38.0404348160504 5 14.644114495637533 36.86254400734732 33.15624521659268 15.337096280422472 6 35.85137138975787 33.378258665059455 16.12115298146926 14.649216963713418 7 31.44078735137572 30.72255384837922 19.153518441063255 18.683140359181806 8 28.364002349686103 34.0990350596893 18.52379248521401 19.013170105410595 9 27.284239482349264 15.3426512302165 18.6342247102604 38.738884577173835 10-11 26.770746457473905 25.320329769043425 26.0042394941077 21.90468427937497 12-13 26.729292053770198 22.15132459486273 26.926409908319727 24.192973443047347 14-15 23.40446701591558 23.79457367530107 24.431434085885076 28.36952522289827 16-17 23.973986271340046 26.96230053028633 25.10731256276484 23.956400635608787 18-19 23.945496696294015 25.87998052906749 26.681453554095746 23.493069220542754 20-21 24.477834514237433 25.11715288077843 26.799175892719113 23.605836712265024 22-23 23.978987646804338 25.027998038367404 26.774620239831204 24.21839407499705 24-25 23.882013084482708 25.15743248984066 27.018798514734392 23.941755910942238 26-27 23.732422985641435 25.42375021716553 26.83126984625063 24.0125569509424 28-29 23.604668384500307 25.75236865480461 26.926812709315527 23.716150251379556 30-31 23.723794114022727 25.421785509544755 26.787005875874165 24.06741450055835 32-33 23.514350673132082 25.286797242950186 26.936343009387762 24.26250907452997 34-35 24.004042337153546 25.51004304091908 26.752448078116053 23.73346654381132 36-37 24.047848823718336 25.318331368894036 26.714498330354658 23.919321477032966 38-39 23.652320797649217 25.516922654932735 26.871488012711247 23.959268534706798 40-41 23.763952585177556 25.486341216655628 26.92075890305529 23.828947295111526 42-43 23.89145366988656 25.316162192857018 26.672597509276624 24.119786627979796 44-45 23.760439345930358 25.590759195116537 26.665376838581412 23.9834246203717 46-47 23.96677858957916 25.806214843956404 26.451659389275576 23.77534717718886 48-49 24.088752860932956 25.773962709440664 26.294815358254812 23.842469071371568 50-51 23.866397111247338 25.872823394438115 26.345820129409663 23.914959364904885 52-53 24.136460742835336 25.76694460766662 26.174498320786473 23.92209632871157 54-55 23.971111365149387 25.786180680773473 26.206752785641758 24.035955168435382 56-57 23.734854254257176 26.133466997317246 26.153447431860954 23.978231316564624 58-59 24.00647356254002 25.710144777184073 26.213471180869945 24.06991047940596 60-61 23.7994525571284 25.756326446239715 26.473346740202086 23.970874256429802 62-63 23.802781808265664 25.840640880551835 26.416113225663118 23.940464085519388 64-65 23.722413513845336 25.96702927002002 26.230215631056453 24.08034158507819 66-67 23.715956285895455 26.074292002734577 26.177674365534447 24.032077345835518 68-69 23.790372396625393 25.784036126232767 26.366093786716043 24.05949769042579 70-71 23.797644505883355 25.731663929834646 26.244426122056304 24.226265442225696 72-73 23.77476313688302 25.908125774718567 26.161069224970202 24.156041863428214 74-75 23.655502212152175 26.163920995059215 26.02223411806164 24.158342674726974 76-77 23.8786112908506 26.073358560048444 25.957509840416947 24.090520308684006 78-79 23.81685367052304 26.119842568637992 26.109487264512893 23.953816496326077 80-81 23.810554879702927 26.000221327859567 26.112134069350933 24.07708972308657 82-83 23.953289053663752 26.1281430915627 26.044280566161383 23.874287288612162 84-85 23.787129896979312 26.206885554490995 25.868119762439363 24.137864786090336 86-87 23.835159810333302 26.013329695749405 26.11212083951338 24.039389654403912 88-89 23.860456080811684 26.05291358275671 25.908398386948324 24.178231949483283 90-91 23.69200349332087 26.235731506310117 25.987089934561737 24.085175065807277 92-93 23.805902731135916 26.112452795235047 26.0562039134386 24.025440560190436 94-95 23.805108687612332 25.979956023519446 26.17232670932392 24.042608579544307 96-97 23.855918025689576 25.85719954655059 26.13397885721069 24.152903570549146 98-99 23.656172743713096 25.99562179163139 25.9794887199099 24.368716744745612 100-101 23.709474281874932 25.95966256587539 25.926471568395637 24.40439158385404 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 119.0 1 93.0 2 150.0 3 264.0 4 233.5 5 209.5 6 438.5 7 830.5 8 1276.5 9 1489.5 10 1376.0 11 1360.5 12 1520.0 13 1747.0 14 1965.5 15 2184.0 16 2385.0 17 2538.5 18 2658.0 19 2753.5 20 2909.0 21 3194.0 22 3546.5 23 4197.5 24 5136.0 25 6251.5 26 8398.0 27 11106.0 28 13188.0 29 15615.0 30 18965.0 31 23156.0 32 27888.0 33 31043.0 34 33758.5 35 37707.0 36 43265.0 37 48126.0 38 51489.5 39 55219.0 40 58757.0 41 63590.0 42 67963.5 43 72380.0 44 78568.5 45 84660.5 46 89831.5 47 95451.5 48 102390.0 49 104726.0 50 103081.0 51 103294.5 52 106339.0 53 109508.0 54 110498.5 55 106383.5 56 98978.0 57 91117.0 58 85651.5 59 80628.5 60 70661.0 61 59383.0 62 48630.5 63 37807.5 64 28187.5 65 20958.0 66 15131.5 67 11733.5 68 9329.5 69 7321.0 70 5752.5 71 3936.5 72 2916.0 73 2343.0 74 1737.5 75 1304.5 76 924.5 77 512.0 78 305.0 79 191.5 80 123.0 81 82.5 82 63.5 83 52.0 84 45.0 85 38.5 86 30.5 87 30.5 88 29.5 89 33.0 90 33.0 91 24.0 92 22.0 93 23.0 94 25.5 95 25.5 96 24.0 97 24.0 98 20.0 99 13.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03998729590216314 2 0.007155621371966035 3 0.006352048918429743 4 0.0033290858789360696 5 0.004974496140938956 6 0.0040178622676814634 7 0.004323985107123862 8 0.008227051310014426 9 0.011250014349508099 10-11 0.001779339004258934 12-13 0.00660077372547669 14-15 0.007595672953664482 16-17 0.015382672681980462 18-19 0.00780613240578113 20-21 0.002984697684563373 22-23 0.007672203663525081 24-25 0.01775512468765904 26-27 0.00443878117191476 28-29 0.01335460887067458 30-31 0.010408176541041507 32-33 0.009451542667784016 34-35 0.2268178913493512 36-37 0.397270914886371 38-39 0.3934061140384108 40-41 0.41366761947400443 42-43 0.403776025224522 44-45 0.4177046144191511 46-47 0.4244584495643489 48-49 0.43050437564333627 50-51 0.44062556202240055 52-53 0.42929901696303185 54-55 0.417953339226198 56-57 0.4126535875683515 58-59 0.4111421060486046 60-61 0.40882705207532155 62-63 0.40555536422878086 64-65 0.4205936487163887 66-67 0.3968691286596029 68-69 0.4213206904600643 70-71 0.4042352094836855 72-73 0.4121944033091879 74-75 0.42524288934042004 76-77 0.41318930253737574 78-79 0.41322756789230597 80-81 0.41540869312333306 82-83 0.41542782580079823 84-85 0.41305537379511964 86-87 0.4159444080923573 88-89 0.4278640661531456 90-91 0.45130159604795417 92-93 0.44007071437591117 94-95 0.41336149663456206 96-97 0.3892734557059384 98-99 0.3819647729142512 100-101 0.3908614679355459 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2613330.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.67943161993533 #Duplication Level Percentage of deduplicated Percentage of total 1 66.41487068922369 28.345489321275696 2 15.576319900871777 13.295769601989898 3 6.590932304955492 8.438917338629121 4 3.487418077892851 5.95364085542217 5 2.057505457575142 4.390658174211102 6 1.2896909534292684 3.3025966114640197 7 0.9000535430026241 2.6889641550002716 8 0.6395893254223375 2.183784710336257 9 0.4921675015932541 1.890488630682333 >10 2.3216729479983385 17.414178042008547 >50 0.14603710859275013 4.277162909912293 >100 0.07934240961302443 6.0537148789069875 >500 0.0035018655784602817 1.015775386408236 >1k 8.979142508847516E-4 0.7488593837531429 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4783 0.18302319263162325 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3197 0.12233433971216799 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.653070986059931E-5 2 0.0 0.0 0.0 0.0 7.653070986059931E-5 3 0.0 0.0 0.0 0.0 7.653070986059931E-5 4 0.0 0.0 0.0 0.0 7.653070986059931E-5 5 0.0 0.0 0.0 0.0 1.1479606479089897E-4 6 0.0 0.0 0.0 0.0 1.1479606479089897E-4 7 0.0 0.0 0.0 0.0 1.1479606479089897E-4 8 0.0 0.0 0.0 0.0 1.1479606479089897E-4 9 0.0 0.0 0.0 0.0 1.1479606479089897E-4 10-11 0.0 0.0 0.0 1.9132677465149828E-5 1.1479606479089897E-4 12-13 0.0 0.0 0.0 3.8265354930299656E-5 3.826535493029966E-4 14-15 3.8265354930299656E-5 0.0 0.0 5.7398032395449484E-5 5.165822915590454E-4 16-17 3.8265354930299656E-5 0.0 0.0 2.2959212958179794E-4 6.122456788847945E-4 18-19 5.7398032395449484E-5 0.0 0.0 3.0612283944239725E-4 6.122456788847945E-4 20-21 7.653070986059931E-5 0.0 0.0 4.783169366287457E-4 6.122456788847945E-4 22-23 7.653070986059931E-5 0.0 0.0 8.035724535362927E-4 6.313783563499443E-4 24-25 1.1479606479089897E-4 0.0 0.0 0.00110969529297869 7.079090662105436E-4 26-27 1.1479606479089897E-4 0.0 0.0 0.0012627567126998885 8.035724535362927E-4 28-29 2.2959212958179794E-4 0.0 0.0 0.0016071449070725855 8.035724535362927E-4 30-31 3.0612283944239725E-4 0.0 0.0 0.002793370909911875 8.418378084665924E-4 32-33 3.0612283944239725E-4 0.0 0.0 0.004917098108543506 9.375011957923416E-4 34-35 3.0612283944239725E-4 0.0 0.0 0.008207918632549277 9.566338732574914E-4 36-37 3.0612283944239725E-4 0.0 0.0 0.014024252581954824 9.566338732574914E-4 38-39 3.0612283944239725E-4 0.0 0.0 0.024164571638484234 9.757665507226412E-4 40-41 3.0612283944239725E-4 0.0 0.0 0.03568244347250443 9.94899228187791E-4 42-43 3.252555169075471E-4 0.0 0.0 0.04888399092345781 9.94899228187791E-4 44-45 3.443881943726969E-4 0.0 0.0 0.06250645727864448 0.0010714299380483903 46-47 3.443881943726969E-4 0.0 0.0 0.08144780796914282 0.0011479606479089896 48-49 3.443881943726969E-4 0.0 0.0 0.10155625198501528 0.0013010220676301883 50-51 3.443881943726969E-4 0.0 0.0 0.12507031258968443 0.0013392874225604879 52-53 3.443881943726969E-4 0.0 0.0 0.1471685550619325 0.0013966854549559374 54-55 3.443881943726969E-4 0.0 0.0 0.17393517083567708 0.001434950809886237 56-57 3.826535493029966E-4 0.0 0.0 0.20626939575178027 0.001454083487351387 58-59 3.826535493029966E-4 0.0 0.0 0.24304240183979825 0.001454083487351387 60-61 4.0178622676814637E-4 0.0 0.0 0.28419679106733553 0.001454083487351387 62-63 4.209189042332962E-4 0.0 0.0 0.3284124086892968 0.0015306141972119863 64-65 4.5918425916359587E-4 0.0 0.0 0.37348899679718983 0.0016071449070725855 66-67 4.974496140938955E-4 0.0 0.0 0.43207325519547857 0.0016454102620028852 68-69 4.974496140938955E-4 0.0 0.0 0.497889665675594 0.0016454102620028852 70-71 4.974496140938955E-4 0.0 0.0 0.5701155231065346 0.0016454102620028852 72-73 4.974496140938955E-4 0.0 0.0 0.6472202132910885 0.001894135069049833 74-75 4.974496140938955E-4 0.0 0.0 0.7234448003122453 0.002028063811305882 76-77 4.974496140938955E-4 0.0 0.0 0.8132344556561935 0.0020663291662361815 78-79 4.974496140938955E-4 0.0 0.0 0.9188085699088901 0.0020663291662361815 80-81 5.357149690241952E-4 0.0 0.0 1.0374694355477494 0.0020854618437013313 82-83 5.357149690241952E-4 0.0 0.0 1.163458116655761 0.00221939058595738 84-85 5.357149690241952E-4 0.0 0.0 1.2909391466060542 0.00223852326342253 86-87 5.357149690241952E-4 0.0 0.0 1.4301293751650195 0.0022959212958179793 88-89 5.357149690241952E-4 0.0 0.0 1.5857163083116177 0.002353319328213429 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6100 0.0 38.28289 1 TACATGG 10135 0.0 32.802696 2 GTACATG 10630 0.0 32.083878 1 ATCAACG 7195 0.0 31.9903 3 GGTATCA 4075 0.0 31.733843 1 ACATGGG 10115 0.0 31.510986 3 TCAACGC 7335 0.0 31.379713 4 CAACGCA 7480 0.0 30.771416 5 TATCAAC 7670 0.0 30.19324 2 AACGCAG 7750 0.0 29.758781 6 CATGGGG 6060 0.0 26.571686 4 ACGCAGA 9355 0.0 24.400072 7 CGCAGAG 9565 0.0 23.864368 8 CATGGGA 4555 0.0 23.602081 4 GTACCTG 2405 0.0 23.437944 1 AGTACAT 6860 0.0 22.367483 2 GAGTACA 7715 0.0 22.287346 1 TACCTGG 2495 0.0 22.018291 2 TAAGGTG 820 0.0 21.947374 5 ATGGGGA 3065 0.0 19.932972 5 >>END_MODULE