FastQCFastQC Report
Thu 26 May 2016
SRR936291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2613330
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48020.18375023437529897No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35130.1344261918701427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72500.034.0413741
GGTATCA50550.033.677481
TCAACGC87700.027.6975574
ATCAACG87850.027.5962623
TATCAAC89750.027.1229632
CAACGCA89600.027.0572745
AACGCAG93200.026.0630466
TACATGG103200.025.9330522
GTACATG108750.025.3615881
ACATGGG102900.024.9893553
ACGCAGA111300.021.7393287
CGCAGAG112700.021.3008888
GCACCGT7250.020.940316
GAGTACA82050.020.7482811
CATGGGG59950.020.4965934
CATGGGA46350.020.2668634
TACCTGG21100.020.2401772
AGTACAT74550.019.9228252
GTACCTG22650.019.735011
GTACTAG3455.0931703E-1119.2968621
Produced by FastQC (version 0.11.5)