##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936290_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 401122 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23596063043164 33.0 31.0 34.0 31.0 34.0 2 32.34167161113078 34.0 31.0 34.0 31.0 34.0 3 32.38003649762416 34.0 31.0 34.0 31.0 34.0 4 35.8923394877369 37.0 35.0 37.0 35.0 37.0 5 35.92433723405846 37.0 35.0 37.0 35.0 37.0 6 36.04802279605706 37.0 37.0 37.0 35.0 37.0 7 36.00980748999058 37.0 37.0 37.0 35.0 37.0 8 36.029392553886346 37.0 37.0 37.0 35.0 37.0 9 37.76558503397969 39.0 38.0 39.0 35.0 39.0 10-11 37.743582999685884 39.0 38.0 39.0 35.0 39.0 12-13 37.70135021265351 39.0 38.0 39.0 35.0 39.0 14-15 38.99398935984563 40.5 39.0 41.0 36.0 41.0 16-17 38.97465982917915 40.0 39.0 41.0 36.0 41.0 18-19 39.021017296483365 40.0 39.0 41.0 36.0 41.0 20-21 38.97515593759505 40.0 39.0 41.0 36.0 41.0 22-23 38.83393705655636 40.0 38.5 41.0 35.0 41.0 24-25 38.95765502764745 40.5 39.0 41.0 35.0 41.0 26-27 38.882168018707524 40.0 39.0 41.0 35.0 41.0 28-29 38.75388161207812 40.0 38.0 41.0 35.0 41.0 30-31 38.57452720120064 40.0 38.0 41.0 35.0 41.0 32-33 38.32562911034548 40.0 38.0 41.0 35.0 41.0 34-35 38.070739076889325 40.0 38.0 41.0 34.5 41.0 36-37 37.87283420006881 40.0 38.0 41.0 34.0 41.0 38-39 37.70393670753536 40.0 37.0 41.0 34.0 41.0 40-41 37.5619836359013 40.0 37.0 41.0 33.5 41.0 42-43 37.36454495141129 40.0 37.0 41.0 33.0 41.0 44-45 37.123570883671306 40.0 37.0 41.0 33.0 41.0 46-47 36.75044624827359 40.0 37.0 41.0 32.5 41.0 48-49 36.491886508344095 40.0 37.0 41.0 31.0 41.0 50-51 36.12914898709121 39.5 36.5 40.5 30.5 41.0 52-53 36.17172082309123 39.5 37.0 40.5 31.0 41.0 54-55 36.28913771869905 40.0 37.0 41.0 31.0 41.0 56-57 36.19254491152318 40.0 37.0 41.0 30.5 41.0 58-59 36.04536774347954 40.0 36.0 41.0 30.0 41.0 60-61 35.84255288914595 40.0 36.0 41.0 29.0 41.0 62-63 35.589053953659985 39.0 35.5 41.0 28.5 41.0 64-65 35.29263665418502 39.0 35.0 41.0 28.0 41.0 66-67 34.97486425576258 39.0 35.0 40.5 27.5 41.0 68-69 34.589905315589775 38.0 35.0 40.0 26.0 41.0 70-71 34.199155119888715 37.0 35.0 40.0 26.0 41.0 72-73 33.76908272296209 37.0 34.5 39.0 25.5 41.0 74-75 33.314091473417065 36.0 34.0 39.0 23.5 40.5 76-77 32.88642482835646 36.0 34.0 38.5 21.5 40.0 78-79 32.4604471956163 35.5 34.0 37.0 20.5 39.0 80-81 32.02238969690019 35.0 33.5 37.0 18.0 39.0 82-83 31.61522928186437 35.0 33.0 36.0 10.0 38.5 84-85 31.22113471711848 35.0 33.0 36.0 4.5 37.0 86-87 30.904916708632285 35.0 33.0 36.0 2.0 37.0 88-89 30.551470624897163 35.0 32.5 35.0 2.0 36.5 90-91 30.197745324365155 35.0 32.0 35.0 2.0 36.0 92-93 29.906347694716324 35.0 32.0 35.0 2.0 36.0 94-95 29.565001421014053 34.5 31.0 35.0 2.0 36.0 96-97 29.23345141877035 34.0 31.0 35.0 2.0 35.0 98-99 28.898502201325282 34.0 31.0 35.0 2.0 35.0 100-101 25.960844830251144 31.5 24.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21050130357057384 1101 2 0.12461611903685821 1101 3 0.22662811484403278 1101 4 0.12006563479371124 1101 5 0.24887862281600093 1101 6 0.2087294783304472 1101 7 0.09911555655475723 1101 8 0.09143662079218728 1101 9 0.18816383253126645 1101 10-11 0.3120224713194233 1101 12-13 0.14518232018944843 1101 14-15 0.30199961082636406 1101 16-17 0.3185716490556061 1101 18-19 0.2494415012839326 1101 20-21 0.3066240717350297 1101 22-23 0.2948649589349728 1101 24-25 0.17899559950186728 1101 26-27 0.25036429585374265 1101 28-29 0.259675874168245 1101 30-31 0.2950228105725685 1101 32-33 0.36024901774474216 1101 34-35 0.06787080222812847 1101 36-37 0.058113141194986895 1101 38-39 0.12842177515384634 1101 40-41 0.16256844888232536 1101 42-43 0.09140779898289253 1101 44-45 0.19806501137703236 1101 46-47 0.19986909092081362 1101 48-49 0.07793004596666009 1101 50-51 0.2601206540315033 1101 52-53 0.4463352003825136 1101 54-55 0.19623919725815853 1101 56-57 0.16743151575026616 1101 58-59 0.22760601148496562 1101 60-61 0.23836378264756775 1101 62-63 0.32376629341536756 1101 64-65 0.27128990816535037 1101 66-67 0.34387045960876605 1101 68-69 0.39576767641624144 1101 70-71 0.4517327072808186 1101 72-73 0.2775220294359997 1101 74-75 0.32098176985679316 1101 76-77 0.07907146730189396 1101 78-79 0.4058574553865455 1101 80-81 0.462142901231811 1101 82-83 0.5628074932874192 1101 84-85 0.4585657106557761 1101 86-87 0.15146992482870303 1101 88-89 0.06429278264599958 1101 90-91 0.4153157224412958 1101 92-93 0.3148605754259073 1101 94-95 0.4295490856602413 1101 96-97 0.6923700887838891 1101 98-99 0.7815404337086456 1101 100-101 1.1388861521679523 1102 1 0.32665937302920156 1102 2 0.26937480136576397 1102 3 0.2478551322086915 1102 4 0.2824124172247622 1102 5 0.3164539547873986 1102 6 0.27158060192595457 1102 7 0.23129763009919202 1102 8 0.3117507168085325 1102 9 0.5004403902432131 1102 10-11 0.413752553035458 1102 12-13 0.35675024664501365 1102 14-15 0.3341593401419871 1102 16-17 0.39888319144580464 1102 18-19 0.3096591979131418 1102 20-21 0.40378954670948985 1102 22-23 0.4201522418767567 1102 24-25 0.48318035435374895 1102 26-27 0.33851227848908394 1102 28-29 0.25281300389244876 1102 30-31 0.3734191333509074 1102 32-33 0.4209135989500581 1102 34-35 0.27109156320872074 1102 36-37 0.3654739685698587 1102 38-39 0.09104307239592657 1102 40-41 0.02454495552175473 1102 42-43 -0.005853436972167003 1102 44-45 0.30973827491124695 1102 46-47 0.43558474464910546 1102 48-49 0.8422622727286608 1102 50-51 0.8177049236944285 1102 52-53 0.8078967427727051 1102 54-55 0.7663043147249553 1102 56-57 0.8781375933805009 1102 58-59 0.8091943669497255 1102 60-61 0.9217460604820928 1102 62-63 1.1002282443857538 1102 64-65 0.9201024720060076 1102 66-67 1.126030188924389 1102 68-69 0.9952154547614924 1102 70-71 1.0729501485474202 1102 72-73 0.8760281581387517 1102 74-75 0.7811930824206286 1102 76-77 0.7591180709791132 1102 78-79 0.6671093706061626 1102 80-81 0.6235480340203701 1102 82-83 0.7191692144314494 1102 84-85 0.6848967882860109 1102 86-87 0.50993519551308 1102 88-89 0.42074707273793166 1102 90-91 0.662506733677251 1102 92-93 0.7866749268048672 1102 94-95 1.0348223236581973 1102 96-97 1.261429715954474 1102 98-99 0.8961822876412278 1102 100-101 0.786964548490733 1103 1 0.33641727816754496 1103 2 0.23911294365825597 1103 3 0.29886797805849596 1103 4 0.13789660694808248 1103 5 0.003336365854593737 1103 6 0.08815668888247785 1103 7 0.16818004116638718 1103 8 0.1086874757018137 1103 9 0.2268484930100172 1103 10-11 0.18586223682991942 1103 12-13 0.2839982703604349 1103 14-15 0.35917169192064335 1103 16-17 0.450884179588094 1103 18-19 0.24376245878270453 1103 20-21 0.44763836093873977 1103 22-23 0.4013775383194442 1103 24-25 0.43247630297035045 1103 26-27 0.4892657369871145 1103 28-29 0.5429562845248554 1103 30-31 0.5958746669477435 1103 32-33 0.33154848037298024 1103 34-35 0.010530792457728921 1103 36-37 -0.09697662431550924 1103 38-39 0.053617183067864005 1103 40-41 0.1428749950474426 1103 42-43 -0.009929523928690287 1103 44-45 0.06646999427884026 1103 46-47 0.08053286717875352 1103 48-49 0.09386800395396477 1103 50-51 -0.008382032827306318 1103 52-53 -0.1278740082154357 1103 54-55 -0.23715418329876314 1103 56-57 -0.28638809831910095 1103 58-59 -0.23477796506608684 1103 60-61 -0.17509188418193844 1103 62-63 -0.1502658267604957 1103 64-65 -0.26986788372521886 1103 66-67 -0.06770745929695465 1103 68-69 -0.037702035357085606 1103 70-71 -0.07817386331029041 1103 72-73 -0.21574555727626432 1103 74-75 -0.217015909674231 1103 76-77 -0.18079052585883204 1103 78-79 -0.35876760567842325 1103 80-81 -0.28362834937884074 1103 82-83 -0.017676141299780568 1103 84-85 -0.15286259906576305 1103 86-87 -0.10543041713514612 1103 88-89 -0.018358658487365176 1103 90-91 0.17201760324246962 1103 92-93 -0.009150172009363189 1103 94-95 0.0676780548834941 1103 96-97 0.37599246299004463 1103 98-99 0.5259377224238371 1103 100-101 0.5468459714156317 1104 1 0.3282651042544984 1104 2 0.32335207409303734 1104 3 0.27169406501501214 1104 4 0.254744433035043 1104 5 0.34029288759371923 1104 6 0.3082653845346499 1104 7 0.35296264986203596 1104 8 0.18205704091920438 1104 9 0.2295658843143613 1104 10-11 0.32716658465601256 1104 12-13 0.2663352268821697 1104 14-15 0.5208564745293387 1104 16-17 0.4794167882837499 1104 18-19 0.4312624587827045 1104 20-21 0.33079053485178633 1104 22-23 0.3497471035368349 1104 24-25 0.21372630297035045 1104 26-27 0.2216026935088493 1104 28-29 0.2250215019161672 1104 30-31 0.5510377104260087 1104 32-33 0.4619832629816685 1104 34-35 0.4276503576751196 1104 36-37 0.16932772351056968 1104 38-39 0.3104106613287385 1104 40-41 0.3657010820039659 1104 42-43 0.48463569346261437 1104 44-45 0.5596765160179658 1104 46-47 0.6905872150048395 1104 48-49 0.6984875691713484 1104 50-51 0.6111831845639912 1104 52-53 0.6588107743932667 1104 54-55 0.7900197297447207 1104 56-57 0.7638836408113292 1104 58-59 0.8154937740643504 1104 60-61 0.8181146375571942 1104 62-63 0.5453863471525509 1104 64-65 0.7179038554052184 1104 66-67 0.58310775809435 1104 68-69 0.7856675298603051 1104 70-71 0.6365000497331934 1104 72-73 0.6130587905498217 1104 74-75 0.7408645251083783 1104 76-77 0.7281768654455121 1104 78-79 0.7961236986694047 1104 80-81 0.88349121583855 1104 82-83 0.8152042934828252 1104 84-85 0.6161591400646707 1104 86-87 0.7478304524300725 1104 88-89 0.5957717762952406 1104 90-91 0.576908907590294 1104 92-93 0.8712846105993322 1104 94-95 0.362515011405236 1104 96-97 0.5009924629900446 1104 98-99 0.9661551137281847 1104 100-101 1.2642372757634597 1105 1 0.19214310377932975 1105 2 0.18230016152472928 1105 3 0.19608228074321232 1105 4 0.058958616926702234 1105 5 0.10416197763886004 1105 6 0.026191257663661816 1105 7 0.3222843414752461 1105 8 0.3035229473102703 1105 9 0.15318194513641004 1105 10-11 0.2375075207453392 1105 12-13 0.22529222379355218 1105 14-15 0.18095982449370496 1105 16-17 0.16918870560377997 1105 18-19 0.2262404820661459 1105 20-21 0.1973191045833147 1105 22-23 0.10633269393834865 1105 24-25 0.2843710523169918 1105 26-27 0.14976258897095107 1105 28-29 0.14571228357451815 1105 30-31 0.3551627995208051 1105 32-33 0.37490794818005924 1105 34-35 0.0806186993239777 1105 36-37 0.3266171628073167 1105 38-39 0.36651810965375375 1105 40-41 0.1125976132465425 1105 42-43 -0.11872912003227754 1105 44-45 -0.06907289001672012 1105 46-47 -0.3013051584805524 1105 48-49 -0.4626774937889735 1105 50-51 -0.5989617429722429 1105 52-53 -0.5643865408835254 1105 54-55 -0.5343818502030118 1105 56-57 -0.3863153375683268 1105 58-59 -0.514327739359274 1105 60-61 -0.5153968853698672 1105 62-63 -0.7022897635625824 1105 64-65 -0.5072758191017854 1105 66-67 -0.5107758841009442 1105 68-69 -0.7026313534849109 1105 70-71 -0.8123966596989831 1105 72-73 -0.8151976242755552 1105 74-75 -0.7192804024278558 1105 76-77 -0.6400296562936134 1105 78-79 -0.7216061659065041 1105 80-81 -0.5680115164018886 1105 82-83 -0.6444283389714371 1105 84-85 -0.6648532738103583 1105 86-87 -0.9166979391118595 1105 88-89 -0.9785481334220307 1105 90-91 -0.5963730477435156 1105 92-93 -0.9670974278896196 1105 94-95 -0.8686488636244647 1105 96-97 -0.8240847525005712 1105 98-99 -0.7488826030691556 1105 100-101 -0.3831329428157737 1106 1 0.03529448791432088 1106 2 -0.009752321511356854 1106 3 -0.03970106159750486 1106 4 0.031350978663326146 1106 5 -0.046651689568250276 1106 6 0.010248184343538469 1106 7 -0.07961021205865393 1106 8 0.07742254498035805 1106 9 0.015519061571886539 1106 10-11 -0.24049944496654518 1106 12-13 -0.051091911197140405 1106 14-15 -5.840414763937929E-4 1106 16-17 -0.004338586775972431 1106 18-19 0.009350131979076082 1106 20-21 0.03628205420200459 1106 22-23 -0.05596539049087568 1106 24-25 0.15112178792018227 1106 26-27 0.11010006570187159 1106 28-29 0.3141579089873545 1106 30-31 0.27801453794631215 1106 32-33 0.13715048706193755 1106 34-35 0.08279548428764372 1106 36-37 0.26448121132710156 1106 38-39 0.24879157389442952 1106 40-41 0.23446004043683644 1106 42-43 0.0775943652256359 1106 44-45 -0.0659953664473889 1106 46-47 -0.025251294311935624 1106 48-49 -0.18467953546314675 1106 50-51 -0.257844526855024 1106 52-53 -0.3092673431413786 1106 54-55 -0.41179883212819135 1106 56-57 -0.2332197992268874 1106 58-59 -0.22277366501830898 1106 60-61 -0.2358151283291008 1106 62-63 -0.3455447326372294 1106 64-65 -0.3874025229989897 1106 66-67 -0.3117351707159628 1106 68-69 -0.29519725752621895 1106 70-71 -0.26622630000402836 1106 72-73 -0.4377205931100008 1106 74-75 -0.5059104390578852 1106 76-77 -0.344699985963949 1106 78-79 -0.26562976525797666 1106 80-81 -0.45579449844716535 1106 82-83 -0.4071888025515733 1106 84-85 -0.5464812087169832 1106 86-87 -0.6407266426487617 1106 88-89 -0.7611023278614724 1106 90-91 -0.5476581014875812 1106 92-93 -0.695186961307023 1106 94-95 -0.776803550467676 1106 96-97 -0.8876243549745979 1106 98-99 -0.5569427123587722 1106 100-101 -1.010144592368409 1107 1 0.020714096238954482 1107 2 -0.003812730376239415 1107 3 0.0946841063072199 1107 4 -0.04494283925060216 1107 5 -0.04551595379564333 1107 6 0.17364065779187854 1107 7 0.05647855950498126 1107 8 0.21357270766683456 1107 9 0.2357597147734367 1107 10-11 0.1956691350567894 1107 12-13 0.1654729485241475 1107 14-15 0.15211102881644223 1107 16-17 -0.03515693044348467 1107 18-19 0.22821715880699145 1107 20-21 0.2504756818034508 1107 22-23 0.2730756801703009 1107 24-25 0.20752488203277863 1107 26-27 0.20633065075927703 1107 28-29 0.3433570617898525 1107 30-31 0.3151408194860039 1107 32-33 0.30319045268291234 1107 34-35 0.23891310967901092 1107 36-37 0.16468994114477198 1107 38-39 0.4659319753923725 1107 40-41 0.7129654735512077 1107 42-43 0.4478825965473803 1107 44-45 0.5467954739970793 1107 46-47 0.7068915628309256 1107 48-49 0.7573365490110362 1107 50-51 0.7939619069346477 1107 52-53 0.793025615296834 1107 54-55 0.9474083015202837 1107 56-57 0.8299056228370816 1107 58-59 1.0275793339380357 1107 60-61 0.9373155119803158 1107 62-63 0.95810343483776 1107 64-65 0.7958960220314069 1107 66-67 0.9034790843027594 1107 68-69 1.0354246345481855 1107 70-71 1.1197934065463997 1107 72-73 0.865267619794416 1107 74-75 0.9026935268086476 1107 76-77 1.027568109069513 1107 78-79 1.1342643350551285 1107 80-81 0.7319549029893899 1107 82-83 0.8176408370825392 1107 84-85 0.9827716613180115 1107 86-87 0.7433976129838733 1107 88-89 0.9993878802544351 1107 90-91 1.131082152671663 1107 92-93 0.8538568719546866 1107 94-95 1.218576767538341 1107 96-97 1.2722458028005867 1107 98-99 1.5080014217194382 1107 100-101 1.7930431416852493 1108 1 -0.0588447062248747 1108 2 0.033287971528935145 1108 3 0.05480443290799286 1108 4 -0.006194808882462155 1108 5 -0.11698135989792036 1108 6 -0.05422068012532577 1108 7 -0.06624024868868617 1108 8 -0.14876793121011644 1108 9 -0.04858350253067556 1108 10-11 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-1.1415152609750976 2316 60-61 -1.3771247680564542 2316 62-63 -1.0592102864926503 2316 64-65 -0.8105026296433948 2316 66-67 -1.0821772813479527 2316 68-69 -1.0381262694425786 2316 70-71 -0.7702544448549631 2316 72-73 -0.7396459883898245 2316 74-75 -0.8806728074911376 2316 76-77 -1.023995901019351 2316 78-79 -0.9276655143190382 2316 80-81 -0.8997366322627158 2316 82-83 -1.1026146846863156 2316 84-85 -0.9891598381044737 2316 86-87 -1.1097186211344088 2316 88-89 -1.0557944500852372 2316 90-91 -1.2093287093607223 2316 92-93 -1.2944647931797881 2316 94-95 -1.0686730733498564 2316 96-97 -1.5436323764889472 2316 98-99 -1.3876389148904202 2316 100-101 -1.5971815006078494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2134.0 3 250.0 4 177.0 5 145.0 6 218.0 7 218.0 8 216.0 9 332.0 10 419.0 11 669.0 12 1146.0 13 2183.0 14 2279.0 15 2233.0 16 3110.0 17 4732.0 18 4148.0 19 1944.0 20 1636.0 21 1364.0 22 1510.0 23 1640.0 24 1668.0 25 1884.0 26 2105.0 27 2430.0 28 2938.0 29 3516.0 30 4627.0 31 5739.0 32 7788.0 33 10535.0 34 15902.0 35 28905.0 36 39014.0 37 80396.0 38 129376.0 39 31582.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.216296030249524 24.069418255464768 14.863867189448584 20.85041852483713 2 13.977231929152707 24.236825833732247 41.34952928035743 20.436412956757618 3 15.840806587932596 31.00023186411267 32.19794712999599 20.961014417958747 4 10.059360423226604 20.371769269699758 42.76269044075301 26.80617986632063 5 9.461683063160992 41.86964911668354 37.442346335844746 11.226321484310724 6 24.474068681469365 43.580122563563016 19.14220464824058 12.803604106727034 7 23.547714738763176 36.445503774695084 25.491159135559922 14.515622350981818 8 22.757339649691456 37.51642460886368 22.950819672131146 16.77541606931372 9 25.419020879981247 17.462486348471774 24.496950535339383 32.621542236207596 10-11 21.201416070404626 29.82112138814789 30.808007778414897 18.169454763032586 12-13 22.48873973548819 28.995820020270347 30.48592731846758 18.029512925773883 14-15 20.623730791238298 30.677297789878246 28.624374044002977 20.074597374880483 16-17 16.933800776467596 33.83979932526612 30.50415537331036 18.72224452495593 18-19 16.876558292609953 32.20130647252418 33.916425650742994 17.00570958412287 20-21 17.52062757182409 31.645356599264773 33.97793326626801 16.85608256264312 22-23 18.05978373337921 30.840432932165484 33.295684890009206 17.804098444446108 24-25 17.829272762862768 31.824682101031964 33.25324818623974 17.092796949865534 26-27 16.918309241133088 31.7558302459748 34.06224912614872 17.26361138674339 28-29 17.262229802343356 30.724300521881364 34.839882408384014 17.173587267391266 30-31 17.604620662830243 31.236348948308017 33.89412767909681 17.26490270976493 32-33 16.732447884072137 32.34752927742054 33.83377755393603 17.08624528457129 34-35 17.18451625158717 31.485013147239076 33.89213265214305 17.438337949030704 36-37 18.009707157400705 30.84476812766565 33.01735818058418 18.128166534349464 38-39 17.12611366069584 31.9264216978356 33.61899812798568 17.328466513482883 40-41 16.60297957880103 31.63275658005974 34.81285264298283 16.951411198156393 42-43 17.125832641422242 31.458222971949112 33.348361266504114 18.06758312012453 44-45 17.104511636063027 31.62316190285328 33.17956361632305 18.09276284476064 46-47 17.731086331854428 31.091091743096282 33.067948663348105 18.109873261701185 48-49 17.484300994990598 31.64616265334167 32.762912141941804 18.10662420972593 50-51 17.30258220756133 31.671663369636168 32.32927374707621 18.696480675726296 52-53 17.433786510900187 31.85450382004512 32.75154737194174 17.96016229711295 54-55 18.203201617216237 31.447791795934165 32.09645682851772 18.25254975833188 56-57 18.01712099294244 32.013626657148045 31.617100953740756 18.352151396168757 58-59 17.343162340175315 31.076469122164983 32.569856960898136 19.010511576761562 60-61 17.86340039951657 32.43285554157039 31.48791744159105 18.215826617321987 62-63 17.783969943644333 32.0902943018159 32.36217908578585 17.763556668753914 64-65 17.957556392531348 31.230233335880165 32.33685339131764 18.475356880270848 66-67 17.954619449522905 31.946079290741064 31.33674772721581 18.762553532520222 68-69 17.672294474777576 31.089725523794492 32.33014804154031 18.90783195988762 70-71 18.120454889840367 30.886273782971813 32.56462243416908 18.428648893018735 72-73 18.309040675902803 30.611503673330343 31.988658138562077 19.090797512204773 74-75 17.768118846606708 31.760778615627427 31.341784202219607 19.12931833554626 76-77 17.816840549578547 31.299800859186156 32.18553912052403 18.69781947071127 78-79 17.421961495493782 30.88150571355573 32.182648424701796 19.51388436624869 80-81 18.189139395336078 31.76608506475858 30.837008267505983 19.207767272399355 82-83 18.548406287926515 30.78446279459782 31.3173909939518 19.349739923523863 84-85 17.31303691104952 30.69224343182208 31.808179271147356 20.18654038598104 86-87 18.01513274657722 31.134229541295667 31.684078552005822 19.166559160121295 88-89 18.57818420970217 30.333907488021044 31.878989070182577 19.208919232094203 90-91 18.117062437978007 29.98005272495815 32.18703326884717 19.715851568216674 92-93 18.904233500922725 30.753175031728393 31.336125515390616 19.006465951958265 94-95 18.365164113566312 29.642014876490563 31.88914396931019 20.103677040632935 96-97 18.685053084458065 29.679464785452144 31.53626258869844 20.099219541391353 98-99 18.367582009647972 30.153859251775035 31.318727409082314 20.159831329494676 100-101 18.494784408442612 29.851013506322445 31.49188080579669 20.16232127943826 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3212.0 1 1843.0 2 818.0 3 1102.0 4 690.5 5 553.0 6 1142.0 7 1911.5 8 2394.0 9 2181.0 10 1497.5 11 1073.5 12 1042.5 13 1038.0 14 1025.0 15 971.0 16 939.5 17 943.5 18 929.0 19 907.0 20 954.5 21 1110.5 22 1259.5 23 1482.0 24 1640.0 25 2062.5 26 3343.5 27 4689.0 28 4933.0 29 5578.0 30 7820.5 31 9756.0 32 12303.0 33 15794.5 34 18494.0 35 20684.5 36 24329.0 37 27445.5 38 26210.0 39 23499.5 40 20893.5 41 18100.0 42 15226.5 43 14964.0 44 15089.5 45 13384.0 46 11046.5 47 9318.0 48 10486.0 49 9387.5 50 6875.0 51 5061.0 52 3229.0 53 2351.0 54 1684.0 55 1287.5 56 1036.5 57 827.0 58 636.5 59 494.0 60 414.5 61 319.5 62 229.5 63 147.0 64 95.5 65 85.0 66 76.0 67 73.5 68 70.5 69 54.0 70 35.0 71 24.0 72 19.5 73 18.5 74 12.0 75 11.5 76 7.5 77 4.0 78 5.0 79 5.0 80 5.0 81 3.5 82 2.5 83 1.5 84 1.0 85 1.5 86 1.5 87 1.5 88 1.5 89 2.5 90 2.5 91 2.5 92 1.5 93 1.0 94 3.0 95 3.0 96 2.0 97 1.0 98 0.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04736713518580382 2 0.008476224191143841 3 0.006232517787605766 4 0.0032409092495549983 5 0.004986014230084613 6 0.0059832170761015355 7 0.007479021345126919 8 0.01171713344069884 9 0.016952448382287683 10-11 0.0029916085380507678 12-13 0.010844580950434031 14-15 0.008850175258400187 16-17 0.0186975533628173 18-19 0.010470629883177686 20-21 0.005110664585836728 22-23 0.010719930594681917 24-25 0.022561714391132873 26-27 0.0059832170761015355 28-29 0.018198951939808836 30-31 0.013462238421228453 32-33 0.011218532017690378 34-35 0.2587741385413914 36-37 0.45597100134123786 38-39 0.4536026445819476 40-41 0.47479320505980727 42-43 0.46507047731114226 44-45 0.48164897462617356 46-47 0.4868842895677624 48-49 0.4917456534420949 50-51 0.5049585911518192 52-53 0.49299215699961607 54-55 0.47803411430936227 56-57 0.47479320505980727 58-59 0.4725494986562692 60-61 0.4710536943872438 62-63 0.46669093193591976 64-65 0.4820229256934299 66-67 0.4567189034757505 68-69 0.48451593280847227 70-71 0.46382397375362105 72-73 0.47192624687750856 74-75 0.48663498885625817 76-77 0.4754164568385678 78-79 0.4726741490120213 80-81 0.4757904079058242 82-83 0.47678761075184106 84-85 0.47504250577131146 86-87 0.4790313171553792 88-89 0.49473726198014567 90-91 0.5160524728137574 92-93 0.5064543954208445 94-95 0.4740453029252945 96-97 0.4501124346208884 98-99 0.43964180473771075 100-101 0.44637292394832495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 401122.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.043473058072106 #Duplication Level Percentage of deduplicated Percentage of total 1 69.68724084824073 14.66461575281336 2 10.633811159815188 4.475446372923948 3 4.597796469612605 2.9026081840437574 4 2.7236109465703118 2.2925693429929046 5 1.7687477786992063 1.8610298113790817 6 1.2320815069304585 1.555636439786399 7 0.9442009240611301 1.3908486694821027 8 0.7925601232081507 1.3342574079706424 9 0.606563203411918 1.1487776786114947 >10 5.416419855467361 24.210090695598847 >50 0.9169529676578605 13.678880739525631 >100 0.6575050349484658 24.817636529534656 >500 0.01540101883663073 2.089139962405453 >1k 0.007108162539983414 3.5784624129317266 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3796 0.9463455008700595 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3310 0.8251853550790034 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2855 0.7117535313445784 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2051 0.511315759295177 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1289 0.32134861712895324 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1053 0.26251364921395487 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 986 0.2458105015431714 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 952 0.23733427735202756 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 724 0.18049371512906298 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 703 0.17525840018747413 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 617 0.1538185389981103 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 608 0.15157483259457222 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 581 0.144843713383958 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 559 0.13935909773086494 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 554 0.13811259417334376 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 534 0.13312657994325916 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 533 0.13287727923175494 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 528 0.13163077567423376 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 501 0.12489965646361954 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 490 0.122157348637073 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 489 0.12190804792556878 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 483 0.12041224365654339 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 479 0.11941504081052648 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 475 0.11841783796450954 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 464 0.11567553013796303 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 463 0.11542622942645878 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 452 0.11268392159991224 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 442 0.11019091448486994 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 438 0.10919371163885302 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 437 0.10894441092734877 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 428 0.10670070452381071 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 424 0.1057035016777938 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 417 0.10395839669726417 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 413 0.10296119385124726 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 406 0.10121608887071765 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2465035575211531E-4 0.0 12-13 0.0 0.0 0.0 2.4930071150423063E-4 0.0 14-15 0.0 0.0 0.0 3.7395106725634597E-4 0.0 16-17 0.0 0.0 0.0 0.0022437064035380756 0.0 18-19 0.0 0.0 0.0 0.003614860316811344 0.0 20-21 0.0 0.0 0.0 0.00585856672034942 0.0 22-23 0.0 0.0 0.0 0.0122157348637073 0.0 24-25 0.0 0.0 0.0 0.018697553362817296 0.0 26-27 0.0 0.0 0.0 0.023932868304406142 0.0 28-29 0.0 0.0 0.0 0.03191049107254152 0.0 30-31 0.0 0.0 0.0 0.056341960799956126 0.0 32-33 0.0 0.0 0.0 0.09884773211142744 0.0 34-35 0.0 0.0 0.0 0.16329196603527107 0.0 36-37 0.0 0.0 0.0 0.25927273996439987 0.0 38-39 0.0 0.0 0.0 0.38741330567757437 0.0 40-41 0.0 0.0 0.0 0.5378662850703776 0.0 42-43 0.0 0.0 0.0 0.6719900678596537 0.0 44-45 0.0 0.0 0.0 0.8134682216383046 0.0 46-47 0.0 0.0 0.0 1.0048065177178014 0.0 48-49 0.0 0.0 0.0 1.2069893947477326 0.0 50-51 0.0 0.0 0.0 1.3837435992042322 0.0 52-53 0.0 0.0 0.0 1.554389936228878 0.0 54-55 0.0 0.0 0.0 1.744606379106606 0.0 56-57 0.0 0.0 0.0 1.9617472988267908 0.0 58-59 0.0 0.0 0.0 2.1889848973628974 0.0 60-61 0.0 0.0 0.0 2.429061482541471 0.0 62-63 0.0 0.0 0.0 2.649692612222715 0.0 64-65 0.0 0.0 0.0 2.89687426767916 0.0 66-67 0.0 0.0 0.0 3.1732241063816 0.0 68-69 0.0 0.0 0.0 3.471013806273403 0.0 70-71 0.0 0.0 0.0 3.8152980888607457 0.0 72-73 0.0 0.0 0.0 4.1461201330268596 0.0 74-75 0.0 0.0 0.0 4.48554305173987 0.0 76-77 0.0 0.0 0.0 4.8700893992351455 0.0 78-79 0.0 0.0 0.0 5.338774736863099 0.0 80-81 0.0 0.0 0.0 5.842860775524653 0.0 82-83 0.0 0.0 0.0 6.3302436665154245 0.0 84-85 0.0 0.0 0.0 6.833706453398218 0.0 86-87 0.0 0.0 0.0 7.379176410169475 0.0 88-89 0.0 0.0 0.0 7.93873185714072 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCCG 80 4.3473847E-9 47.33169 9 GGCATTC 90 1.2312739E-8 42.072613 7 GTATAGT 360 0.0 40.762928 1 AATATAG 60 8.456111E-5 39.443073 5 CATATGC 50 0.0016429619 37.865353 3 CGTAATC 25 0.001643942 37.865353 22-23 AGGGGTG 385 0.0 36.881836 8 GGTATGC 55 0.002622891 34.42305 8 GACTGGT 55 0.002622891 34.42305 7 TAGGCAT 115 1.0662552E-7 32.926395 5 TATACTC 145 6.87578E-10 32.642548 5 GTAGGGG 425 0.0 32.29692 6 AGTCCCG 30 0.0039198953 31.716583 50-51 GTGTGGC 30 0.003922301 31.712606 62-63 ACGTGTT 30 0.003922301 31.712606 36-37 TCCCGTG 30 0.003922301 31.712606 52-53 TACGTGT 30 0.003922301 31.712606 36-37 CCGTACG 30 0.003924708 31.708633 32-33 CGTACGT 30 0.003924708 31.708633 34-35 GTACGTG 30 0.003924708 31.708633 34-35 >>END_MODULE