##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936290_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 401122 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28319812924746 33.0 31.0 34.0 30.0 34.0 2 32.43398517159368 34.0 31.0 34.0 31.0 34.0 3 32.501505776297485 34.0 31.0 34.0 30.0 34.0 4 36.06079696451454 37.0 35.0 37.0 35.0 37.0 5 36.035507900339546 37.0 35.0 37.0 35.0 37.0 6 36.08829233998634 37.0 35.0 37.0 35.0 37.0 7 36.02946485109268 37.0 35.0 37.0 35.0 37.0 8 36.018099231655206 37.0 35.0 37.0 35.0 37.0 9 37.74091174256211 39.0 38.0 39.0 35.0 39.0 10-11 37.68397395306167 39.0 37.0 39.0 35.0 39.0 12-13 37.65289238685487 39.0 37.0 39.0 35.0 39.0 14-15 38.879468092001936 40.0 38.0 41.0 35.0 41.0 16-17 38.800889754239364 40.0 38.0 41.0 34.5 41.0 18-19 38.90604853386252 40.0 38.0 41.0 35.0 41.0 20-21 38.880283305328554 40.0 38.0 41.0 35.0 41.0 22-23 38.74208470240974 40.0 38.0 41.0 34.5 41.0 24-25 38.85971225711879 40.0 38.0 41.0 35.0 41.0 26-27 38.7137541695544 40.0 38.0 41.0 35.0 41.0 28-29 38.58915118093747 40.0 38.0 41.0 34.0 41.0 30-31 38.41884638588758 40.0 38.0 41.0 34.0 41.0 32-33 38.163950119913636 40.0 37.0 41.0 34.0 41.0 34-35 37.988936034423446 40.0 37.0 41.0 33.5 41.0 36-37 37.80529988382587 40.0 37.0 41.0 33.0 41.0 38-39 37.635452555581594 40.0 36.0 41.0 33.0 41.0 40-41 37.50892621197541 40.0 36.0 41.0 33.0 41.0 42-43 37.43117181306435 40.0 37.0 41.0 33.0 41.0 44-45 37.1915788712661 40.0 37.0 41.0 33.0 41.0 46-47 36.86812740263561 40.0 36.5 41.0 31.5 41.0 48-49 36.54985390978306 40.0 36.0 41.0 30.5 41.0 50-51 36.34568036657177 40.0 36.0 41.0 30.0 41.0 52-53 36.166161417224686 40.0 36.0 41.0 30.0 41.0 54-55 36.01102283095916 40.0 36.0 41.0 29.0 41.0 56-57 35.795509844885096 39.5 35.0 41.0 28.0 41.0 58-59 35.583220317010785 39.0 35.0 41.0 28.0 41.0 60-61 35.35386241592333 39.0 35.0 41.0 27.0 41.0 62-63 35.08620943254172 39.0 35.0 40.5 26.5 41.0 64-65 34.74306570070951 38.0 35.0 40.0 26.0 41.0 66-67 34.39955300382427 38.0 34.5 40.0 26.0 41.0 68-69 34.02635607122023 37.0 34.0 40.0 24.5 41.0 70-71 33.65674906886184 37.0 34.0 39.0 23.5 41.0 72-73 33.22758911253933 36.0 34.0 39.0 22.5 40.5 74-75 32.75801626437842 36.0 33.0 38.5 21.5 40.0 76-77 31.98618998708622 35.0 32.0 37.0 20.5 39.0 78-79 32.01278912650017 35.0 33.0 37.0 20.0 39.0 80-81 31.781224914115903 35.0 33.0 37.0 19.5 39.0 82-83 31.42898295281735 35.0 32.5 36.0 18.0 38.5 84-85 31.067518111696693 35.0 32.0 36.0 14.0 37.0 86-87 30.653025762735524 35.0 32.0 35.0 6.5 37.0 88-89 30.098612142939057 34.5 31.0 35.0 2.0 36.0 90-91 29.828613239862186 34.0 31.0 35.0 2.0 36.0 92-93 29.524395321124246 34.0 31.0 35.0 2.0 36.0 94-95 29.340163341826177 34.0 31.0 35.0 2.0 35.5 96-97 29.083773764590326 34.0 31.0 35.0 2.0 35.0 98-99 28.67833851047811 34.0 30.0 35.0 2.0 35.0 100-101 25.54105608767407 30.5 24.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17131738762559934 1101 2 0.1679191393117776 1101 3 0.08454663583599142 1101 4 0.16611835202344594 1101 5 0.23278483774375047 1101 6 0.05134773148617455 1101 7 0.2707449592503224 1101 8 0.13903837849847633 1101 9 0.11261414448816964 1101 10-11 0.3201695027086444 1101 12-13 0.28869283321576233 1101 14-15 0.4992146388376071 1101 16-17 0.581364489147937 1101 18-19 0.512960845706921 1101 20-21 0.31754181985760965 1101 22-23 0.5329416035297427 1101 24-25 0.3919281337395901 1101 26-27 0.5582334569325056 1101 28-29 0.4338954669448043 1101 30-31 0.3759514658990426 1101 32-33 0.514200284225204 1101 34-35 0.547642777188905 1101 36-37 0.511500962150258 1101 38-39 0.7076916967563491 1101 40-41 0.571027913592836 1101 42-43 0.6891580176857417 1101 44-45 1.0134960075072925 1101 46-47 0.7829828422853709 1101 48-49 0.4792022857655951 1101 50-51 0.6046534574525282 1101 52-53 0.7876191761807902 1101 54-55 0.5622078841958071 1101 56-57 0.7880262161659459 1101 58-59 0.7367600896294348 1101 60-61 0.8178240307887776 1101 62-63 0.8026335525057604 1101 64-65 0.6175629340585331 1101 66-67 0.7022373227686174 1101 68-69 0.9372730813752312 1101 70-71 0.8989648688423131 1101 72-73 0.9308501429311278 1101 74-75 0.7990651301881471 1101 76-77 0.6350492641900409 1101 78-79 0.5690231528167367 1101 80-81 0.4716452285524504 1101 82-83 0.646447847038015 1101 84-85 0.9529026638932976 1101 86-87 1.0544171225995385 1101 88-89 0.7631174760896862 1101 90-91 1.1487996329703272 1101 92-93 0.8865730971022536 1101 94-95 0.9311582383220021 1101 96-97 0.8968402104428002 1101 98-99 1.1178300721512393 1101 100-101 1.5654916023400318 1102 1 0.07718434370323024 1102 2 0.2715963609665266 1102 3 0.3356957676030987 1102 4 0.34219087500608225 1102 5 0.3723724271206663 1102 6 0.2435400195352031 1102 7 0.34543852411242426 1102 8 0.37981340403473496 1102 9 0.39826914959542137 1102 10-11 0.4541231543940327 1102 12-13 0.6092846360758202 1102 14-15 0.6270874682553753 1102 16-17 0.5987610672888977 1102 18-19 0.6103018824791349 1102 20-21 0.3739609975899896 1102 22-23 0.5447840447963443 1102 24-25 0.5499656720439745 1102 26-27 0.7975401474330184 1102 28-29 0.5822611462093619 1102 30-31 0.5770654853066048 1102 32-33 0.7507299062885338 1102 34-35 0.721512797617919 1102 36-37 0.5963132706282934 1102 38-39 0.5755255833753452 1102 40-41 0.4131180565958985 1102 42-43 0.5486606989932028 1102 44-45 0.5355848736972817 1102 46-47 0.474951688046346 1102 48-49 0.4016678118125725 1102 50-51 0.14552743600206952 1102 52-53 -0.01358102810930717 1102 54-55 0.15994792505383515 1102 56-57 0.10057409665623851 1102 58-59 0.05610699974179312 1102 60-61 0.1567061809419883 1102 62-63 0.08120224504917672 1102 64-65 0.4288020811474027 1102 66-67 0.3997124249136661 1102 68-69 0.40144251447022583 1102 70-71 0.27444367374528156 1102 72-73 0.28331439216503895 1102 74-75 0.4132760597080676 1102 76-77 0.10362370239228724 1102 78-79 0.12365479224471798 1102 80-81 0.3181403766627646 1102 82-83 0.545084849080915 1102 84-85 0.386109379930069 1102 86-87 0.5279551716291593 1102 88-89 0.6062769500426946 1102 90-91 0.5615932999774813 1102 92-93 0.7105644147733479 1102 94-95 0.8458830851044361 1102 96-97 0.6833091838850862 1102 98-99 0.5658158995261111 1102 100-101 0.7825977055065252 1103 1 0.012090470185441404 1103 2 0.12312798151988602 1103 3 0.11385782293906743 1103 4 0.03858415563848894 1103 5 0.027881320401299092 1103 6 0.054187252736788594 1103 7 -0.041419183397458426 1103 8 0.062249174785719674 1103 9 0.15703891244127277 1103 10-11 0.2102372848288141 1103 12-13 0.18259244239992967 1103 14-15 0.3646121718126949 1103 16-17 0.2977482214391003 1103 18-19 0.32738439236055683 1103 20-21 0.2398824402777393 1103 22-23 0.3712415546777663 1103 24-25 0.37592911077916114 1103 26-27 0.4367446928875651 1103 28-29 0.13315047427260396 1103 30-31 0.3002003667295341 1103 32-33 0.4006804991739088 1103 34-35 0.16148315334914543 1103 36-37 0.26676781608284017 1103 38-39 0.1331466308061806 1103 40-41 -0.017959018502914148 1103 42-43 0.06786771480347653 1103 44-45 0.0628823045273208 1103 46-47 0.19814832836255647 1103 48-49 0.3389207762394548 1103 50-51 -0.045245785341805345 1103 52-53 -0.06953458542155744 1103 54-55 -0.03348092870110975 1103 56-57 0.03653012432422287 1103 58-59 0.1451015649591838 1103 60-61 -0.05000083486828544 1103 62-63 -0.15842226087968214 1103 64-65 -0.16550374889212804 1103 66-67 -0.10627818773455999 1103 68-69 0.17460209945046046 1103 70-71 0.3187865591207668 1103 72-73 0.2944309929555544 1103 74-75 0.3028511585222944 1103 76-77 0.28772696326185 1103 78-79 0.23562366576250682 1103 80-81 0.2735504557141475 1103 82-83 0.28378297161055954 1103 84-85 0.2953855657008191 1103 86-87 0.211873155818882 1103 88-89 0.25665985518103795 1103 90-91 0.2862721537324191 1103 92-93 0.6538697507417268 1103 94-95 0.3715130258158972 1103 96-97 0.31936402578231693 1103 98-99 0.14616421968421278 1103 100-101 0.6231980019492163 1104 1 0.041981774533269345 1104 2 0.28888885108510465 1104 3 0.06494477946080934 1104 4 0.16901893824718428 1104 5 0.1392943638795643 1104 6 0.05146986143243737 1104 7 0.021080816602541574 1104 8 -0.013837781736015131 1104 9 0.021169347223882085 1104 10-11 0.1545307630896886 1104 12-13 0.21520113805210173 1104 14-15 0.13363391094312504 1104 16-17 0.17138952578692823 1104 18-19 0.2512974358388149 1104 20-21 0.23852374462556725 1104 22-23 0.26254590250385235 1104 24-25 0.5376138933878494 1104 26-27 0.4448968668006117 1104 28-29 0.6290743873160807 1104 30-31 0.3966677580338782 1104 32-33 0.230843542652174 1104 34-35 0.3598527185665361 1104 36-37 0.6920395552132774 1104 38-39 0.6005379351540014 1104 40-41 0.6369322858449067 1104 42-43 0.8776503234991253 1104 44-45 0.6511975219186255 1104 46-47 0.4264091979277751 1104 48-49 0.31718164580467345 1104 50-51 0.3243194320494922 1104 52-53 0.3149762841436612 1104 54-55 0.21651907129889025 1104 56-57 0.3368018634546601 1104 58-59 0.6668406953939652 1104 60-61 0.5532600346969332 1104 62-63 0.6866864347724899 1104 64-65 0.21357233806439524 1104 66-67 0.3380152905263074 1104 68-69 0.4748738385808977 1104 70-71 0.617699602599032 1104 72-73 0.7998657755642498 1104 74-75 0.5542098541744664 1104 76-77 0.5771291371748966 1104 78-79 0.27910192663207667 1104 80-81 0.27490915136632665 1104 82-83 0.47807644987142695 1104 84-85 0.215222522222561 1104 86-87 0.3300796775580146 1104 88-89 0.15204028996364727 1104 90-91 0.48871780590633307 1104 92-93 0.22180453335042216 1104 94-95 0.13374128668545993 1104 96-97 0.45387489534753556 1104 98-99 0.08638161098856045 1104 100-101 0.3039045236883453 1105 1 -0.12284359966488267 1105 2 -0.034911338983796725 1105 3 0.002548723390482621 1105 4 -0.03454782345392715 1105 5 -0.258573300648834 1105 6 0.15072146513877982 1105 7 0.05973310930864528 1105 8 0.2213279345861494 1105 9 0.1757933671811145 1105 10-11 0.21940662433939195 1105 12-13 0.1094010667762646 1105 14-15 0.41102313641236066 1105 16-17 0.402241569027602 1105 18-19 0.30692228734748994 1105 20-21 0.1777907914300343 1105 22-23 0.1793313622330075 1105 24-25 0.34869580120443544 1105 26-27 0.4568949661116122 1105 28-29 0.2752566752704695 1105 30-31 0.40401065420874005 1105 32-33 0.33688862461938385 1105 34-35 0.30719026905358504 1105 36-37 0.6825806576128954 1105 38-39 0.37792716062323706 1105 40-41 0.33184942768382797 1105 42-43 0.126692554432843 1105 44-45 0.08880146584829873 1105 46-47 0.17660966122072352 1105 48-49 0.10073326139031735 1105 50-51 0.1723608314317744 1105 52-53 0.10607868376352059 1105 54-55 0.2629968569962031 1105 56-57 0.16837557454993402 1105 58-59 0.15472380302048094 1105 60-61 0.09250629271546273 1105 62-63 0.28107494748335426 1105 64-65 0.35283493012902056 1105 66-67 0.21787095695538738 1105 68-69 0.24814985426157676 1105 70-71 0.0562835892942104 1105 72-73 0.07041697537417946 1105 74-75 0.0941317477358865 1105 76-77 0.15664624337589217 1105 78-79 -0.07735893105384051 1105 80-81 -0.002168242313885571 1105 82-83 0.2234954923993442 1105 84-85 0.08549307342237 1105 86-87 0.0128502287578236 1105 88-89 0.03707830374364107 1105 90-91 0.33730119863619734 1105 92-93 0.044052989040611834 1105 94-95 -0.14932773208384376 1105 96-97 -0.3042327311670725 1105 98-99 0.03182589704225336 1105 100-101 0.27846053461730946 1106 1 0.31216810993699085 1106 2 0.2805873699575372 1106 3 0.2471593040666349 1106 4 -0.012329604514398795 1106 5 0.07545059894884076 1106 6 -0.15003515528997013 1106 7 -0.07426677059287101 1106 8 -0.010702330230998314 1106 9 0.11783083790711402 1106 10-11 0.15587452802040502 1106 12-13 0.23183396175014792 1106 14-15 0.2628891665570805 1106 16-17 0.1740919863698167 1106 18-19 0.10079576359801479 1106 20-21 0.14935747611147576 1106 22-23 0.05674083800313667 1106 24-25 0.1920423453706519 1106 26-27 -7.553071124348776E-4 1106 28-29 0.032935471883689615 1106 30-31 0.225994681110798 1106 32-33 0.2119561800148162 1106 34-35 0.24845460580971945 1106 36-37 0.45735847542349717 1106 38-39 0.5814415424163073 1106 40-41 0.6072052194330624 1106 42-43 0.8935814032889056 1106 44-45 0.8952252333376336 1106 46-47 0.763350788945452 1106 48-49 0.783005224399993 1106 50-51 0.9212048835545161 1106 52-53 1.0363690218407484 1106 54-55 1.283886486492392 1106 56-57 1.1588127330198787 1106 58-59 1.1685726590824501 1106 60-61 1.248942070052884 1106 62-63 0.9396875097844415 1106 64-65 0.9606608473811704 1106 66-67 0.8024503120265507 1106 68-69 0.8101431887958981 1106 70-71 0.7098520154036194 1106 72-73 0.5170181883688372 1106 74-75 0.601525310457319 1106 76-77 0.7700041968977835 1106 78-79 0.6103399820549136 1106 80-81 0.6490282388006285 1106 82-83 0.7806773815484398 1106 84-85 0.6796127515584409 1106 86-87 0.5863655112894968 1106 88-89 0.5582156363563833 1106 90-91 0.60042946381364 1106 92-93 0.4701173618262935 1106 94-95 0.36806402916037584 1106 96-97 0.13674637169727433 1106 98-99 -0.11948317639796358 1106 100-101 0.26509957863340006 1107 1 0.05977385614609432 1107 2 0.04711692978318638 1107 3 0.06163533083316253 1107 4 0.05860783793142588 1107 5 0.0496356317930875 1107 6 -0.07805406375580759 1107 7 0.03709672624548688 1107 8 -0.007264427351756808 1107 9 0.0769290023125393 1107 10-11 -0.05434402437691688 1107 12-13 0.030524796055509285 1107 14-15 -0.10764462925605045 1107 16-17 0.08747678592779806 1107 18-19 0.10407251477979429 1107 20-21 -0.13198269210020186 1107 22-23 -0.003606635752156251 1107 24-25 0.20459682999217677 1107 26-27 0.19198212305516194 1107 28-29 0.402855356468379 1107 30-31 0.0780574228383486 1107 32-33 0.19470527449575314 1107 34-35 0.043170364910956494 1107 36-37 0.1646379278146881 1107 38-39 0.24333760724532993 1107 40-41 0.1919556645187015 1107 42-43 0.2875589341379481 1107 44-45 0.307105950385953 1107 46-47 0.19838363319617258 1107 48-49 0.37835331206650835 1107 50-51 0.43162602665722005 1107 52-53 0.4245220699099974 1107 54-55 0.22924071327120288 1107 56-57 0.15601943693049236 1107 58-59 -0.047332246068258144 1107 60-61 0.009462973730208546 1107 62-63 0.5462701729313508 1107 64-65 0.22432804610423318 1107 66-67 0.37051771947943024 1107 68-69 0.706983080747527 1107 70-71 0.5356009871023133 1107 72-73 0.3853649254306575 1107 74-75 0.26805640506103856 1107 76-77 0.36209088116323684 1107 78-79 0.4025003356677779 1107 80-81 0.5736476138389968 1107 82-83 0.640414013631446 1107 84-85 0.5381138872942124 1107 86-87 0.18614143369111957 1107 88-89 0.6202513659898763 1107 90-91 0.22309660114558483 1107 92-93 0.6148622817108809 1107 94-95 0.6434722801272876 1107 96-97 0.24343646930915597 1107 98-99 0.25024486092783604 1107 100-101 0.8693724007833197 1108 1 -0.028308080539197533 1108 2 0.10842619779636209 1108 3 0.06034611725345229 1108 4 0.11147991929512813 1108 5 -0.07912815562991682 1108 6 0.019195613940802048 1108 7 0.052839456513353866 1108 8 0.09955408002541333 1108 9 0.2377942078704791 1108 10-11 0.06814559029147205 1108 12-13 0.0150757199919056 1108 14-15 0.13166206032997252 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-1.2328566078531438 2316 60-61 -1.0001131995720058 2316 62-63 -0.9693227837195266 2316 64-65 -0.8453216722089465 2316 66-67 -1.2399176575566102 2316 68-69 -1.1580547525033111 2316 70-71 -1.1716509752765916 2316 72-73 -1.53167889513837 2316 74-75 -1.1027624903494697 2316 76-77 -1.1844169094220582 2316 78-79 -1.251219344694288 2316 80-81 -1.0779446827925447 2316 82-83 -1.017996518391275 2316 84-85 -1.3122208367941326 2316 86-87 -1.3342307700662737 2316 88-89 -1.3014774132100833 2316 90-91 -1.2419164813804038 2316 92-93 -1.3899582166037128 2316 94-95 -1.1099869283209607 2316 96-97 -1.3303619419300254 2316 98-99 -1.4763362389472299 2316 100-101 -0.992377121881276 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 6.0 5 13.0 6 44.0 7 64.0 8 135.0 9 241.0 10 373.0 11 682.0 12 1105.0 13 1544.0 14 1905.0 15 2547.0 16 3507.0 17 4865.0 18 4347.0 19 2279.0 20 1842.0 21 1802.0 22 1878.0 23 2156.0 24 2443.0 25 2677.0 26 3143.0 27 3599.0 28 4510.0 29 5644.0 30 7037.0 31 8517.0 32 10469.0 33 13247.0 34 18324.0 35 30332.0 36 39242.0 37 78698.0 38 118558.0 39 23341.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.26565604808978 24.570278815751653 15.417223839934014 20.746841296224552 2 13.94424496309595 24.57485537602234 40.98269499301816 20.498204667863554 3 15.771261611180638 31.11472320142002 32.561165929567565 20.55284925783178 4 10.006681259068314 20.763508359052857 42.58779124555622 26.642019136322613 5 9.293930524877718 42.04431569447699 37.44122735726288 11.220526423382413 6 24.58030225218263 43.80138710915881 18.867825748774685 12.750484889883875 7 23.042116862201524 36.790801800948344 25.200063820982145 14.967017515867989 8 22.667916494233676 37.78950045123429 22.663179780715094 16.879403273816944 9 25.159926406429967 17.303214483374134 24.550136866090615 32.98672224410528 10-11 21.074465154485996 29.921432586965764 30.694143031832084 18.30995922671616 12-13 22.3093252293881 29.061518766757704 30.548724015042822 18.08043198881137 14-15 20.47382093228494 30.695150103958397 28.690897033820136 20.140131929936526 16-17 16.80831172716394 33.99269545273235 30.41658356601516 18.782409254088552 18-19 16.698410957264873 32.07540349320157 34.178130344384 17.048055205149556 20-21 17.36491640947143 31.55847846789755 34.107453592672556 16.969151529958467 22-23 17.85865646860556 30.900698540593634 33.48569761818101 17.7549473726198 24-25 17.755999296950083 31.7961803447687 33.39935852927941 17.048461829001806 26-27 16.814859782677797 31.803784253427057 34.21409864388652 17.167257320008623 28-29 17.114065933791974 30.80167882238114 34.91072997859713 17.173525265229753 30-31 17.698371543393932 31.184619910449847 34.02045792239651 17.09655062375971 32-33 16.720495717567633 32.16991116025184 34.01189931578311 17.09769380639741 34-35 17.109165134469755 31.264046869643803 34.26968743183022 17.357100564056218 36-37 17.883711190111736 30.65002667517613 33.27391666375816 18.192345470953974 38-39 17.100283704209694 31.78359701038587 33.830106551124096 17.28601273428034 40-41 16.478027717460407 31.449644619177853 35.128353431276004 16.94397423208574 42-43 17.0067052017357 31.24282453662556 33.72894729953267 18.021522962106072 44-45 17.079880878626092 31.356869236992445 33.397240363148164 18.1660095212333 46-47 17.62553514036448 31.077411778199437 33.12733909635144 18.169713985084638 48-49 17.273032143208887 31.59466868795418 32.991077495016384 18.141221673820546 50-51 17.349136856382078 31.428574990276548 32.496783780279834 18.72550437306154 52-53 17.431077147469658 31.76304126956424 32.791507981525 18.0143736014411 54-55 18.109974159949044 31.307845627820996 32.25082674451507 18.331353467714894 56-57 18.148546583014735 31.879687813585665 31.5134509164025 18.45831468699709 58-59 17.271730052478716 31.217231965895063 32.50264886628523 19.008389115340986 60-61 17.884045578008276 32.588575082675895 31.375205832748502 18.152173506567326 62-63 17.627554709041142 32.36833182921904 32.18808741480148 17.816026046938337 64-65 17.78472500090372 31.43053379354532 32.1303252522004 18.654415953350558 66-67 17.77389422669412 32.128504544751976 31.00565414013692 19.09194708841699 68-69 17.56086676871376 31.31117216208535 32.07677464711484 19.05118642208605 70-71 18.00599318910456 31.210579324993393 32.2950374200368 18.48839006586525 72-73 18.164639010769847 30.76261467889908 31.802827084164342 19.26991922616673 74-75 17.505148059692562 32.129377097242234 31.07944715074217 19.286027692323035 76-77 17.673967857111634 31.52645625217768 31.9128502866734 18.88672560403728 78-79 17.061971504113174 31.38390590043783 31.820490137238927 19.733632458210067 80-81 17.881227266721346 32.02889915461496 30.569232571880168 19.520641006783524 82-83 18.140989524384104 31.236506598989834 30.97947756542897 19.643026311197094 84-85 17.001189173287727 31.132905696314563 31.36500639461108 20.500898735786638 86-87 17.589027761311378 31.66673946549024 31.20673409917696 19.53749867402143 88-89 18.100456827082674 30.914556787467596 31.35847135929876 19.62651502615097 90-91 17.551359265422782 30.67640068166445 31.727779326762583 20.044460726150184 92-93 18.17230606910294 31.465852935918704 30.967438685796505 19.39440230918185 94-95 17.56099628406636 30.35684680406023 31.54230567387291 20.5398512380005 96-97 17.878703642530635 30.402247770864992 31.130978276451383 20.58807031015299 98-99 17.38532092937447 31.0338723811637 30.864840809905548 20.71596587955628 100-101 17.224770642201833 30.91094934184284 31.183810331072998 20.68046968488233 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3345.0 1 1919.5 2 754.0 3 1000.0 4 671.0 5 568.5 6 1148.0 7 1897.5 8 2325.5 9 2085.0 10 1384.5 11 912.0 12 844.0 13 820.0 14 782.0 15 788.0 16 846.0 17 839.0 18 823.5 19 902.0 20 1013.0 21 1142.0 22 1351.5 23 1561.0 24 1716.5 25 2197.0 26 3566.5 27 4872.0 28 4975.0 29 5741.5 30 8068.0 31 9914.5 32 12266.5 33 15920.5 34 18762.5 35 20774.0 36 24354.5 37 27395.0 38 25945.0 39 23360.5 40 21232.5 41 18383.0 42 15338.5 43 15230.0 44 15363.5 45 13474.5 46 10980.5 47 9271.0 48 10652.0 49 9548.5 50 6988.5 51 5159.5 52 3086.5 53 1988.0 54 1356.0 55 1103.5 56 913.0 57 722.0 58 553.0 59 436.5 60 350.5 61 272.5 62 202.0 63 129.0 64 79.0 65 67.0 66 61.0 67 58.5 68 53.5 69 43.0 70 34.0 71 28.5 72 23.5 73 22.0 74 13.5 75 7.0 76 6.0 77 5.5 78 3.5 79 1.5 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25852483782988717 2 0.020442658343346912 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.7395106725634597E-4 12-13 1.2465035575211531E-4 14-15 0.0 16-17 4.986014230084613E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003116258893802883 26-27 0.003116258893802883 28-29 0.0018697553362817297 30-31 0.0014958042690253839 32-33 0.0016204546247774992 34-35 0.002368356759290191 36-37 0.0 38-39 0.0 40-41 7.479021345126919E-4 42-43 0.0038641610283155748 44-45 0.004612063162828267 46-47 0.014708741978749607 48-49 0.014334790911493261 50-51 0.007479021345126919 52-53 0.00984737810441711 54-55 1.2465035575211531E-4 56-57 0.0038641610283155748 58-59 0.0017451049805296145 60-61 0.0018697553362817297 62-63 0.0 64-65 6.232517787605767E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.986014230084613E-4 74-75 0.0 76-77 0.18784808611843778 78-79 0.09909703282293168 80-81 7.479021345126919E-4 82-83 0.0 84-85 7.479021345126919E-4 86-87 0.11829318760875744 88-89 0.5694028250756628 90-91 0.30377291696790504 92-93 0.28756837072013003 94-95 0.0028669581822986525 96-97 0.003365559605307113 98-99 0.003365559605307113 100-101 4.986014230084613E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 401122.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.451383868249557 #Duplication Level Percentage of deduplicated Percentage of total 1 68.58535990735662 14.026655232074031 2 11.142804900347413 4.557715607720344 3 4.782105199000427 2.9340200736932904 4 2.6537453525934054 2.17091059577884 5 1.8370207838117876 1.8784808611843777 6 1.3262631803498506 1.6274350446996175 7 1.005668312305723 1.439711608936932 8 0.7996586822697629 1.3083301339742024 9 0.653379655025294 1.2026266322964085 >10 5.537880173096849 23.9834763488415 >50 0.9703175473883099 14.001725160923609 >100 0.6838544523678918 25.228484102093628 >500 0.014627902724446883 1.9809434536126165 >1k 0.007313951362223441 3.659485144170602 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3743 0.9331325631603352 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3556 0.8865133301090442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2859 0.7127507341905954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2158 0.5379909354261297 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1281 0.31935421143691944 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1082 0.2697433698475775 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 935 0.23309616525645566 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 909 0.22661434675734565 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 725 0.1807430158405672 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 683 0.17027238595738953 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 653 0.16279336461226263 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 619 0.15431714042111877 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 608 0.15157483259457222 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 605 0.15082693046005954 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 583 0.14534231480696644 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 562 0.1401069998653776 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 543 0.13537028634679724 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 521 0.12988567069370416 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 483 0.12041224365654339 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 481 0.11991364223353494 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 476 0.11866713867601378 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 475 0.11841783796450954 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 469 0.11692203369548418 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 467 0.1164234322724757 No Hit GTATGAGATGGAGGCTAGTTGGCCAATGATAATAAATGGGTGTTCTACTG 463 0.11542622942645878 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 461 0.11492762800345033 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 455 0.11343182373442494 No Hit GCTCATGAGTGGAGGACGTCTTCAGATGAAATTAATATACGGATTGGAAG 455 0.11343182373442494 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 450 0.11218532017690377 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 447 0.11143741804239109 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 440 0.10969231306186147 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 435 0.10844580950434032 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 429 0.10695000523531495 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 421 0.1049555995432811 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 414 0.1032104945627515 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 407 0.10146538958222186 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 404 0.10071748744770917 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 403 0.10046818673620495 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2465035575211531E-4 0.0 12-13 0.0 0.0 0.0 2.4930071150423063E-4 0.0 14-15 0.0 0.0 0.0 3.7395106725634597E-4 0.0 16-17 0.0 0.0 0.0 0.0022437064035380756 0.0 18-19 0.0 0.0 0.0 0.003614860316811344 0.0 20-21 0.0 0.0 0.0 0.00585856672034942 0.0 22-23 0.0 0.0 0.0 0.012963636998219993 0.0 24-25 0.0 0.0 0.0 0.019320805141577874 0.0 26-27 0.0 0.0 0.0 0.02493007115042306 0.0 28-29 0.0 0.0 0.0 0.032658393207054215 0.0 30-31 0.0 0.0 0.0 0.05659126151146035 0.0 32-33 0.0 0.0 0.0 0.0992216831786838 0.0 34-35 0.0 0.0 0.0 0.16316731567951895 0.0 36-37 0.0 0.0 0.0 0.2588987888971435 0.0 38-39 0.0 0.0 0.0 0.38666540354306167 0.0 40-41 0.0 0.0 0.0 0.5368690822243607 0.0 42-43 0.0 0.0 0.0 0.673361221772927 0.0 44-45 0.0 0.0 0.0 0.8170830819551159 0.0 46-47 0.0 0.0 0.0 1.0080474269673565 0.0 48-49 0.0 0.0 0.0 1.2114768075548088 0.0 50-51 0.0 0.0 0.0 1.3904747184148465 0.0 52-53 0.0 0.0 0.0 1.5634894121987823 0.0 54-55 0.0 0.0 0.0 1.756323512547305 0.0 56-57 0.0 0.0 0.0 1.9734644322674897 0.0 58-59 0.0 0.0 0.0 2.2021978350726212 0.0 60-61 0.0 0.0 0.0 2.4425237209626998 0.0 62-63 0.0 0.0 0.0 2.6652739066917297 0.0 64-65 0.0 0.0 0.0 2.91220626143667 0.0 66-67 0.0 0.0 0.0 3.1873095965815885 0.0 68-69 0.0 0.0 0.0 3.4849746461176396 0.0 70-71 0.0 0.0 0.0 3.8301314811952474 0.0 72-73 0.0 0.0 0.0 4.160205623226849 0.0 74-75 0.0 0.0 0.0 4.499254590872603 0.0 76-77 0.0 0.0 0.0 4.884424190146639 0.0 78-79 0.0 0.0 0.0 5.349868618525037 0.0 80-81 0.0 0.0 0.0 5.851710950783053 0.0 82-83 0.0 0.0 0.0 6.335478981457014 0.0 84-85 0.0 0.0 0.0 6.8369473626477735 0.0 86-87 0.0 0.0 0.0 7.375187598785407 0.0 88-89 0.0 0.0 0.0 7.930878884728337 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCTGCG 25 0.0050469665 56.919746 9 TGGGCGA 25 0.0050469665 56.919746 6 ATGGGCG 35 2.8052018E-4 54.209282 5 CATTCCG 55 4.9988983E-5 43.12102 9 CCTGTAC 90 1.2081728E-8 42.162773 3 GTACTGG 50 0.0016010312 38.065125 1 CCCTGGC 65 1.3376855E-4 36.48702 7 CCTTTAG 80 1.06330135E-5 35.68605 1 TGTACAC 55 0.002595503 34.496815 5 TAGGCAT 100 1.3107492E-6 33.203186 5 ATAGCCG 60 0.003975236 31.622082 8 TAGCCGA 60 0.003975236 31.622082 9 TATCTGG 60 0.003975236 31.622082 6 GGCATTC 75 3.1004165E-4 31.622082 7 TGGGATA 60 0.003975236 31.622082 6 TGGGTAT 140 1.7234925E-8 30.492722 6 GGGTATG 125 2.1746928E-7 30.357197 7 TGGACTG 110 2.7577244E-6 30.184713 5 CAGAGAT 65 0.0058799386 29.189613 4 CATGGGC 395 0.0 28.820124 4 >>END_MODULE