FastQCFastQC Report
Thu 26 May 2016
SRR936289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712064
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107261.5063252741326623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73731.0354406345497034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55120.7740877224519145No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20650.2900020222901312No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17580.2468879201869495No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16370.2298950656120798No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13810.19394324105698363No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.17624820240877223No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11350.15939578464857093No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10670.1498460812511235No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA9160.12864012223620347No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG8050.11305163580801725No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7860.11038333632931872No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7560.10617023188926838No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG407.688399E-659.1834532
TAACGCA905.8430487E-736.8226744
ATGGGCG753.1374456E-431.5622925
GTATCAA105050.030.7468511
ATGGGTA3400.030.6339875
GGTATCA71100.029.17541
ACTAGCG650.005939651329.1323788
GTACATG83600.027.9854971
CGAGCAA350.00834164327.17651694-95
GTAAGCG350.00834164327.17651694-95
TACATGG85400.027.1107352
GAGTACG700.00851105527.0628991
GTACCGT700.00853149427.0495936
CATGGGT6750.026.6526014
CTGGTCG1458.115785E-726.1186819
ACATGGG85800.025.7133353
ATCAACG126500.025.3745843
TCAACGC126650.025.3445324
CAACGCA127250.025.2250295
TATCAAC128100.025.2072542
Produced by FastQC (version 0.11.5)