##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936289_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 712064 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.041947072173286 33.0 31.0 34.0 30.0 34.0 2 32.10329408592486 33.0 31.0 34.0 30.0 34.0 3 32.15254948993349 34.0 31.0 34.0 30.0 34.0 4 35.68375033704835 37.0 35.0 37.0 35.0 37.0 5 35.713258920546465 37.0 35.0 37.0 35.0 37.0 6 35.851162255078194 37.0 35.0 37.0 35.0 37.0 7 35.80930927557073 37.0 35.0 37.0 35.0 37.0 8 35.82208762133741 37.0 35.0 37.0 35.0 37.0 9 37.49803388459464 39.0 38.0 39.0 35.0 39.0 10-11 37.45026008898077 39.0 37.5 39.0 35.0 39.0 12-13 37.355087464048175 39.0 37.0 39.0 35.0 39.0 14-15 38.48032690881718 40.0 38.0 41.0 34.0 41.0 16-17 38.493718963463955 40.0 38.0 41.0 34.5 41.0 18-19 38.598899115809814 40.0 38.0 41.0 34.5 41.0 20-21 38.60031752763797 40.0 38.0 41.0 34.5 41.0 22-23 38.44428169378033 40.0 38.0 41.0 34.0 41.0 24-25 38.540575706678055 40.0 38.0 41.0 35.0 41.0 26-27 38.45790336261909 40.0 38.0 41.0 34.0 41.0 28-29 38.311202363832464 40.0 38.0 41.0 34.0 41.0 30-31 38.03263892009707 40.0 37.5 41.0 34.0 41.0 32-33 37.699674467463595 40.0 36.5 41.0 33.5 41.0 34-35 37.367259824959554 40.0 36.0 41.0 33.0 41.0 36-37 37.11087978046917 40.0 35.0 41.0 33.0 41.0 38-39 36.90113809994607 40.0 35.0 41.0 32.0 41.0 40-41 36.6529047950746 40.0 35.0 41.0 31.5 41.0 42-43 36.34498022649649 39.5 35.0 41.0 31.0 41.0 44-45 35.96067277660435 39.0 35.0 41.0 29.5 41.0 46-47 35.3874771087992 39.0 35.0 41.0 27.0 41.0 48-49 34.96696434590149 39.0 35.0 41.0 24.0 41.0 50-51 34.54795566128887 38.5 34.5 40.5 23.0 41.0 52-53 34.52299863495416 39.0 35.0 40.5 23.0 41.0 54-55 34.57573406323027 39.0 35.0 41.0 22.0 41.0 56-57 34.4147288726856 39.0 35.0 41.0 21.5 41.0 58-59 34.19514748674276 38.0 35.0 41.0 20.5 41.0 60-61 33.90843028154772 38.0 34.0 40.0 19.5 41.0 62-63 33.62465101451555 37.0 34.0 40.0 18.0 41.0 64-65 33.300754567005214 36.5 34.0 40.0 14.0 41.0 66-67 32.95500544894841 36.0 34.0 39.5 10.0 41.0 68-69 32.596087570780156 35.5 33.0 39.0 7.5 41.0 70-71 32.188867854574866 35.0 33.0 39.0 3.5 40.5 72-73 31.811064033570016 35.0 33.0 37.5 2.0 40.0 74-75 31.417498988854934 35.0 32.5 37.0 2.0 39.5 76-77 31.036029064803166 35.0 32.0 36.5 2.0 39.0 78-79 30.6669519874618 35.0 32.0 36.0 2.0 39.0 80-81 30.290211975328063 35.0 31.0 36.0 2.0 37.0 82-83 29.967243815162682 35.0 31.0 35.0 2.0 37.0 84-85 29.668186427062736 35.0 31.0 35.0 2.0 36.5 86-87 29.35851763324645 34.5 31.0 35.0 2.0 36.0 88-89 29.03557264515549 34.0 30.0 35.0 2.0 36.0 90-91 28.694446004853496 34.0 30.0 35.0 2.0 35.5 92-93 28.406036536041704 34.0 29.0 35.0 2.0 35.0 94-95 28.06471890167176 34.0 29.0 35.0 2.0 35.0 96-97 27.690402828959193 34.0 29.0 35.0 2.0 35.0 98-99 27.264390560399065 34.0 28.0 35.0 2.0 35.0 100-101 24.38296838486428 30.5 14.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2633101131629587 1101 2 0.20262759114513074 1101 3 0.3766232806396843 1101 4 0.17270615527713318 1101 5 0.2807928967618736 1101 6 0.24786230001678433 1101 7 0.38597222339042503 1101 8 0.33256112450673214 1101 9 0.33070093529703115 1101 10-11 0.4467299938456293 1101 12-13 0.26630415104570204 1101 14-15 0.437374304012323 1101 16-17 0.47481055142819173 1101 18-19 0.4546838590065718 1101 20-21 0.43229775639218104 1101 22-23 0.5485632695960305 1101 24-25 0.48879172478081756 1101 26-27 0.4758551162475939 1101 28-29 0.255019531387326 1101 30-31 0.42594811146255296 1101 32-33 0.5148720922022605 1101 34-35 0.2806444756112114 1101 36-37 0.3549338288062316 1101 38-39 0.3399108024837858 1101 40-41 0.151381163432184 1101 42-43 0.2849977974567608 1101 44-45 0.44171615918146045 1101 46-47 0.436903638753563 1101 48-49 0.2392957613380986 1101 50-51 0.5950043547240327 1101 52-53 0.6309506201450787 1101 54-55 0.5992123454555269 1101 56-57 0.40144245965351644 1101 58-59 0.535694203731353 1101 60-61 0.36602429651904345 1101 62-63 0.3509039897233066 1101 64-65 0.3187778824259553 1101 66-67 0.488976215356395 1101 68-69 0.4283400808965112 1101 70-71 0.5913971960084723 1101 72-73 0.6093044892701442 1101 74-75 0.4504065631322618 1101 76-77 0.4204703802220955 1101 78-79 0.542258772002512 1101 80-81 0.5736135136464462 1101 82-83 0.7399794904807351 1101 84-85 0.7941894053318777 1101 86-87 0.8414035546385463 1101 88-89 0.8182920824265416 1101 90-91 0.648479991313252 1101 92-93 0.7975882766777111 1101 94-95 0.9494431486444981 1101 96-97 1.1996591913618495 1101 98-99 1.1539046052622481 1101 100-101 1.2695235934161282 1102 1 0.19516561398398125 1102 2 0.1839351101431035 1102 3 0.3027316550239263 1102 4 0.0508708935060298 1102 5 0.08831189473796996 1102 6 0.11659487724976714 1102 7 0.08775173230706912 1102 8 0.189112084906931 1102 9 0.1828222168489475 1102 10-11 0.24053989952401622 1102 12-13 0.2717362356678379 1102 14-15 0.26667892080691047 1102 16-17 0.22238568412762305 1102 18-19 0.320342391866383 1102 20-21 0.25897702473143625 1102 22-23 0.3083009256849323 1102 24-25 0.3351276930093334 1102 26-27 0.32880696258356323 1102 28-29 0.2765378419994633 1102 30-31 0.3105950736957226 1102 32-33 0.16436420661017337 1102 34-35 0.06937552536872715 1102 36-37 0.08501936743226679 1102 38-39 0.23142185758746336 1102 40-41 0.2955748473211415 1102 42-43 0.1675922145339399 1102 44-45 -0.00817309884732964 1102 46-47 0.12829631450870238 1102 48-49 -0.09053392514755387 1102 50-51 -0.3540638852423683 1102 52-53 -0.01746271471572669 1102 54-55 -0.08415153738710757 1102 56-57 0.029406716727635285 1102 58-59 -0.08578782933849283 1102 60-61 0.00676206716860861 1102 62-63 0.018114819524349457 1102 64-65 -0.08041828361062642 1102 66-67 -0.11354602850506268 1102 68-69 -0.1539049261680674 1102 70-71 -0.11447785649849607 1102 72-73 0.1546323236917999 1102 74-75 0.15648209679400438 1102 76-77 -0.04061718230893163 1102 78-79 -0.07195820588728097 1102 80-81 -0.12279119097798485 1102 82-83 -0.06982117397109278 1102 84-85 -0.04727773485218023 1102 86-87 -0.09142571179136283 1102 88-89 -0.12784531399152854 1102 90-91 -0.1948583063153393 1102 92-93 -0.21167204332840228 1102 94-95 -0.03865208945073917 1102 96-97 -0.1440991620195966 1102 98-99 0.22678427838249604 1102 100-101 0.18174148779120003 1103 1 0.2931591259388071 1103 2 0.2994944036670759 1103 3 0.24421909945501596 1103 4 0.2678119003545234 1103 5 0.24435338618736324 1103 6 0.21900186191074766 1103 7 0.20222968496836558 1103 8 0.06991628211146406 1103 9 0.2685044628339668 1103 10-11 0.2838015439631292 1103 12-13 0.1431928418595163 1103 14-15 0.10905947269124994 1103 16-17 0.5204673053379238 1103 18-19 0.18230722826351808 1103 20-21 0.33632093117542894 1103 22-23 0.2784919625706621 1103 24-25 0.3439466284893271 1103 26-27 0.3529328787195709 1103 28-29 0.30434832700086645 1103 30-31 0.33657528916290147 1103 32-33 0.3853782640413854 1103 34-35 -0.032506808725557335 1103 36-37 0.20826419101350524 1103 38-39 0.3328476255397206 1103 40-41 0.35058706246791616 1103 42-43 0.360955985610083 1103 44-45 0.4344517559726384 1103 46-47 0.2841175328194083 1103 48-49 0.43447308354321734 1103 50-51 0.5441859445465766 1103 52-53 0.4544144129225387 1103 54-55 0.6340109841395858 1103 56-57 0.5164466462402331 1103 58-59 0.43539844163752406 1103 60-61 0.4672370561549499 1103 62-63 0.5845411882215359 1103 64-65 0.5341237485092023 1103 66-67 0.670470151558213 1103 68-69 0.472095314129902 1103 70-71 0.5614522968917086 1103 72-73 0.5088075009115585 1103 74-75 0.5763066792762821 1103 76-77 0.4675249138903581 1103 78-79 0.47750713113062204 1103 80-81 0.3983670452349166 1103 82-83 0.4244877691183362 1103 84-85 0.5724947830700415 1103 86-87 0.40662787465583605 1103 88-89 0.1314001503842981 1103 90-91 0.21068456684738024 1103 92-93 0.16836320037378982 1103 94-95 0.2170970394691203 1103 96-97 0.3724733879423532 1103 98-99 0.5332282689307775 1103 100-101 0.46442000915769555 1104 1 0.31576725602010214 1104 2 0.23329602968333774 1104 3 0.3331947092111136 1104 4 0.4041240954764689 1104 5 0.40763143496785403 1104 6 0.25731080500017356 1104 7 0.31667521342365035 1104 8 0.2710089650382983 1104 9 0.3404524303136398 1104 10-11 0.48579178786556554 1104 12-13 0.4960383703148068 1104 14-15 0.5504188222847475 1104 16-17 0.5400185248501188 1104 18-19 0.31360478923912893 1104 20-21 0.45499410190713263 1104 22-23 0.6524822064731097 1104 24-25 0.6129352463755069 1104 26-27 0.6579800331911159 1104 28-29 0.687912554643141 1104 30-31 0.7134338257482682 1104 32-33 0.599771759976349 1104 34-35 0.6983029473720066 1104 36-37 0.6561471178427709 1104 38-39 0.6522297393608554 1104 40-41 0.5916602331996259 1104 42-43 0.7211120831710502 1104 44-45 0.8538078535336169 1104 46-47 0.893437858022665 1104 48-49 0.7302844656570358 1104 50-51 0.5456103347904744 1104 52-53 0.5596762015404337 1104 54-55 0.6056402524322664 1104 56-57 0.5183328251020143 1104 58-59 0.7300716123692226 1104 60-61 0.5883817716021014 1104 62-63 0.6620176109857638 1104 64-65 0.6465400086718063 1104 66-67 0.7749124279809791 1104 68-69 0.7068530377071411 1104 70-71 0.6740961993307266 1104 72-73 0.5426546553831031 1104 74-75 0.4314351345608358 1104 76-77 0.6508810114513359 1104 78-79 0.7882388384476933 1104 80-81 0.608461354178008 1104 82-83 0.6925788260289067 1104 84-85 0.5078053521757298 1104 86-87 0.5288002323794103 1104 88-89 0.42948145119730796 1104 90-91 0.712219526196975 1104 92-93 0.7591404361461471 1104 94-95 0.3679002915016376 1104 96-97 0.6972538757472329 1104 98-99 0.8524933095811811 1104 100-101 0.5536232611902143 1105 1 0.2973344484491278 1105 2 0.3258184271596747 1105 3 0.15219786378524702 1105 4 0.34726100399382176 1105 5 0.3596415296050779 1105 6 0.25368935389607117 1105 7 0.3191130683132428 1105 8 0.2218051795493352 1105 9 0.209731925581778 1105 10-11 0.2850056679917472 1105 12-13 0.30266297599303726 1105 14-15 0.20111661408285642 1105 16-17 0.3075604806860781 1105 18-19 0.34155936337162274 1105 20-21 0.2776843227273247 1105 22-23 0.2670777900693153 1105 24-25 0.2685112716121054 1105 26-27 0.24519391331730134 1105 28-29 0.31480971552642245 1105 30-31 0.4209909235400673 1105 32-33 0.3603660817429102 1105 34-35 -0.021565822344086882 1105 36-37 -0.19747559509097812 1105 38-39 0.031611442830893566 1105 40-41 0.018448876732755082 1105 42-43 -0.2155655193526016 1105 44-45 -0.026612335740828996 1105 46-47 -0.16711608519500487 1105 48-49 -0.30964991920100715 1105 50-51 -0.28082752009910905 1105 52-53 -0.145588151771868 1105 54-55 0.03749640385182573 1105 56-57 0.13764512794113415 1105 58-59 0.10026341047647946 1105 60-61 0.14682593563995994 1105 62-63 0.0373854343296145 1105 64-65 0.04499805283583669 1105 66-67 -0.07838599473195274 1105 68-69 -0.06012109478497507 1105 70-71 0.11604951163356958 1105 72-73 0.3387656963925565 1105 74-75 0.325462894813203 1105 76-77 0.3055144499371387 1105 78-79 0.23246596778523454 1105 80-81 0.26096482420955525 1105 82-83 0.19674034022449405 1105 84-85 0.14738642473093577 1105 86-87 0.21432452260023283 1105 88-89 0.27867388021938666 1105 90-91 0.17507378487205116 1105 92-93 0.12522119324394154 1105 94-95 0.2926157489148906 1105 96-97 0.5043769041384003 1105 98-99 0.46615766289348315 1105 100-101 0.4699071728621398 1106 1 -0.04104239707189805 1106 2 -0.1529869265760908 1106 3 -0.08168311884318058 1106 4 0.08219589034826669 1106 5 0.036561751709932366 1106 6 0.04996238871058267 1106 7 -0.0802189042234076 1106 8 -0.032228440693231164 1106 9 -0.13957411568937772 1106 10-11 -0.031778046221916156 1106 12-13 -0.046495566336780314 1106 14-15 -0.0846387946081677 1106 16-17 -0.19709882054957717 1106 18-19 -0.07562236008214285 1106 20-21 -0.0410252042768775 1106 22-23 0.009669536789843391 1106 24-25 -0.058065658601869075 1106 26-27 -0.012012123671631514 1106 28-29 0.038123112712519 1106 30-31 -0.036613836393513566 1106 32-33 -0.07950787803270032 1106 34-35 -0.04890308581049396 1106 36-37 -0.11534247491740501 1106 38-39 -0.1246993707447217 1106 40-41 -0.10790537766010289 1106 42-43 -0.12762879164041152 1106 44-45 -0.14930647527482677 1106 46-47 -0.31066229280689583 1106 48-49 -0.5755809943731265 1106 50-51 -0.6469555777283773 1106 52-53 -0.7137826219889831 1106 54-55 -0.7854644232841679 1106 56-57 -0.5848225293474556 1106 58-59 -0.576336834086284 1106 60-61 -0.4061654380509907 1106 62-63 -0.4989742442178553 1106 64-65 -0.6068283171200548 1106 66-67 -0.4302435297867433 1106 68-69 -0.4979300693818516 1106 70-71 -0.5250760480297529 1106 72-73 -0.41948591776924005 1106 74-75 -0.19158869651005617 1106 76-77 -0.4711352781414284 1106 78-79 -0.46141425355834187 1106 80-81 -0.48461496558066486 1106 82-83 -0.5079810533074429 1106 84-85 -0.35128846381220313 1106 86-87 -0.39575964695693955 1106 88-89 -0.5284129681088778 1106 90-91 -0.4706052098512927 1106 92-93 -0.6204275879865797 1106 94-95 -0.6343211262962463 1106 96-97 -0.4650206340566889 1106 98-99 -0.5316358005243984 1106 100-101 -0.5367719122637844 1107 1 -0.07406338648401345 1107 2 0.08450130151199886 1107 3 0.19728696621276498 1107 4 0.10281009218157777 1107 5 0.04494807417707847 1107 6 0.1083684656262065 1107 7 0.0463437471963033 1107 8 0.06077898151276173 1107 9 0.1308207993416488 1107 10-11 0.1704652639775901 1107 12-13 0.06803178052896897 1107 14-15 0.2759792835697539 1107 16-17 0.179650814800695 1107 18-19 0.2296733230117809 1107 20-21 0.19771041162706382 1107 22-23 0.22940609444674465 1107 24-25 0.23934315411850804 1107 26-27 0.21220210897365632 1107 28-29 0.2481998693054166 1107 30-31 0.054154748318282486 1107 32-33 0.2226159115414248 1107 34-35 0.03733688476904007 1107 36-37 0.09336886413601775 1107 38-39 -0.01425460989779026 1107 40-41 0.05184474720292798 1107 42-43 -0.3057294324796871 1107 44-45 -0.11015787958005063 1107 46-47 -0.049260000296939666 1107 48-49 0.023755305854734843 1107 50-51 0.11714773182507088 1107 52-53 0.07825148984355934 1107 54-55 0.07995063134495695 1107 56-57 0.003855065629203125 1107 58-59 0.13715036030992422 1107 60-61 0.11950664804361821 1107 62-63 0.058283838333373694 1107 64-65 -0.19158981010908605 1107 66-67 -0.08425989491852448 1107 68-69 0.027319594544039205 1107 70-71 -0.0028342784287431755 1107 72-73 -0.31149493275528783 1107 74-75 -0.21045481601577265 1107 76-77 -0.10212590782378683 1107 78-79 0.03571824536696866 1107 80-81 -0.13021146295543318 1107 82-83 0.13281474036169172 1107 84-85 -0.05230799214057669 1107 86-87 -0.3488683013932423 1107 88-89 -0.2348941665951152 1107 90-91 -0.11133764019511716 1107 92-93 -0.3136608818110176 1107 94-95 0.026814294796533744 1107 96-97 0.27891450177688526 1107 98-99 0.3440478400589413 1107 100-101 0.4830334753582548 1108 1 7.815209400447998E-4 1108 2 0.14593882444316364 1108 3 0.008979279807910245 1108 4 0.20726587131344587 1108 5 0.2565094553463112 1108 6 0.11622201315155678 1108 7 0.06337215665509177 1108 8 0.15858742209797327 1108 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1709.0 12 2768.0 13 4340.0 14 4902.0 15 5502.0 16 7788.0 17 11549.0 18 10171.0 19 4880.0 20 3826.0 21 3620.0 22 3662.0 23 3685.0 24 4312.0 25 4717.0 26 5534.0 27 6560.0 28 7880.0 29 9624.0 30 11945.0 31 15032.0 32 19358.0 33 26089.0 34 38066.0 35 63134.0 36 94480.0 37 144786.0 38 151413.0 39 31617.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.82760185509103 18.498927323535238 13.799101115386831 19.874369705986897 2 15.610924234018498 21.127536007977586 40.56081854507405 22.700721212929867 3 20.21895913652292 28.320447187870872 29.11412138959698 22.346472286009227 4 11.99593277277879 16.261324966679915 37.06471469721207 34.67802756332923 5 12.46116638483621 40.08738616764886 31.674325713611566 15.77712173390336 6 29.91062023712884 36.14992879393498 17.500540716667278 16.438910252268904 7 28.092148967516405 35.820994043715004 19.2994307752159 16.78742621355269 8 28.423212620366524 32.54601175294105 19.008640546522994 20.02213508016944 9 30.03192599573852 15.820152564965117 19.83133860236334 34.31658283693302 10-11 25.91145796760322 27.386152018268483 24.856821048078352 21.845568966049946 12-13 28.00265730896905 25.415081632896698 26.63622184597299 19.946039212161264 14-15 24.375810214282804 25.182056817176335 27.6841530162092 22.75797995233166 16-17 20.943615530748623 29.312620050074266 27.65806227328752 22.08570214588959 18-19 20.835220645190287 26.171730982743473 32.90221863603709 20.090829736029153 20-21 22.684865286953396 24.69130600871871 32.73687137656734 19.88695732776055 22-23 21.920365168539327 25.088693820224716 32.62387640449438 20.36706460674157 24-25 21.317167693348214 25.284107868634393 33.27838813689431 20.12033630112309 26-27 21.010508870919605 25.493895866323513 33.297847314146075 20.197747948610814 28-29 20.59390740308956 25.706724948240456 33.336938475670905 20.362429172999075 30-31 20.6192263989022 25.7663606140155 33.251939340055536 20.362473647026764 32-33 20.33780585608805 26.05912831453825 32.91616161758034 20.686904211793358 34-35 21.048944993815613 25.724227796577715 32.95112399534534 20.275703214261327 36-37 21.233978710932956 25.4767680287334 32.737918479138735 20.551334781194917 38-39 21.07813125983941 25.73051444241935 32.71141681854859 20.479937479192646 40-41 20.884683300422793 25.896256664119893 32.61416923989703 20.604890795560287 42-43 20.724796140063766 26.13717163736915 31.96862391016055 21.169408312406535 44-45 20.77509579165782 26.40717257455381 31.618055325282434 21.199676308505932 46-47 21.20707330478668 26.72329430922531 30.925917694480255 21.14371469150776 48-49 21.557238184144108 26.92095197170249 30.136738856936823 21.385070987216587 50-51 21.389417350216497 27.365232952653002 29.92840368298342 21.31694601414708 52-53 21.621867624386578 27.720205608729554 29.331559952161047 21.326366814722828 54-55 20.92952869563745 27.481960282991448 29.573847842712293 22.01466317865881 56-57 21.47710758377425 27.82659611992945 28.925502645502643 21.770793650793653 58-59 21.833993054061356 27.219317695212553 29.219290887811734 21.72739836291436 60-61 21.700006560517956 27.173735918694724 29.921746423988637 21.20451109679868 62-63 21.787500238076596 27.367098590516015 29.66695376940501 21.17844740200238 64-65 21.467693265047092 27.546852720572723 29.778032083433576 21.20742193094661 66-67 21.605558717872213 27.862008256820552 29.123173056779923 21.40925996852731 68-69 21.58714973002834 27.870120102640733 29.186783135819766 21.355947031511157 70-71 21.51421289611257 27.86837948929933 29.192287642465793 21.42511997212231 72-73 21.57602231506055 28.075128499250095 28.871026681848132 21.47782250384122 74-75 21.19674461757723 28.580126927459442 28.829748579209717 21.39337987575361 76-77 21.436795150906313 28.087226684688982 28.89364529997136 21.582332864433347 78-79 21.47728843977775 28.089277299162763 28.890961371864588 21.5424728891949 80-81 21.205489734613913 28.674156003391932 28.628511684804465 21.491842577189693 82-83 21.19725906209016 28.594729627625536 28.438112666855265 21.769898643429038 84-85 21.165606084122306 28.421190015944013 28.25603544368095 22.157168456252734 86-87 21.415151910656146 28.337113609346392 28.19305158224834 22.05468289774912 88-89 21.274304837718443 28.188566803950444 28.246354406639856 22.290773951691257 90-91 21.18649630013868 28.252119877624043 28.346689532762387 22.21469428947489 92-93 21.31088658402853 28.473109204559726 28.330353072679316 21.885651138732428 94-95 21.03829550673485 28.210687016438364 28.395169727621685 22.3558477492051 96-97 21.142404143732914 28.13932624843778 28.37764449321932 22.34062511460999 98-99 21.258922942169605 28.291209852931015 27.770184784770386 22.679682420129 100-101 21.2424339455766 28.42593624413371 27.89075823467429 22.440871575615407 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1158.0 1 812.5 2 892.0 3 1335.5 4 1008.0 5 856.0 6 1871.0 7 3879.0 8 5867.0 9 5986.5 10 4239.5 11 2852.5 12 2638.5 13 2724.0 14 2694.0 15 2652.0 16 2670.0 17 2670.5 18 2619.5 19 2587.0 20 2647.0 21 2897.5 22 3098.5 23 3355.5 24 3647.5 25 4025.0 26 4725.0 27 5375.0 28 5836.5 29 6512.5 30 7813.5 31 9222.0 32 10974.0 33 12577.0 34 13927.0 35 14713.0 36 15761.0 37 17172.5 38 17156.0 39 16920.0 40 16997.5 41 17806.0 42 18556.5 43 19424.5 44 20740.0 45 21666.0 46 22298.5 47 23796.5 48 29433.0 49 30194.0 50 25506.0 51 22726.5 52 20846.5 53 20914.0 54 20531.0 55 19326.0 56 18084.0 57 16611.5 58 15569.0 59 14392.5 60 13360.5 61 12195.0 62 10582.0 63 7901.0 64 5357.0 65 4308.5 66 3138.0 67 2254.0 68 1691.5 69 1141.5 70 855.5 71 772.0 72 733.5 73 734.0 74 568.5 75 461.5 76 337.5 77 162.0 78 93.5 79 50.5 80 28.0 81 21.5 82 17.0 83 9.5 84 9.0 85 7.0 86 3.5 87 2.5 88 4.0 89 3.5 90 4.0 91 4.5 92 5.0 93 6.5 94 8.0 95 8.0 96 7.0 97 5.0 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04114798669782491 2 0.009690140212115764 3 0.008285772065432321 4 0.004353541254718677 5 0.0073027143627539095 6 0.006460093474743842 7 0.00603878303073881 8 0.0101114506561208 9 0.014745865540176165 10-11 0.0025278626640302 12-13 0.009198611360776559 14-15 0.00856664569476901 16-17 0.017063072982203848 18-19 0.01018166906345497 20-21 0.004493978069387021 22-23 0.008987956138774043 24-25 0.02001224609023908 26-27 0.006530311882078015 28-29 0.016571544130864643 30-31 0.01334149739349272 32-33 0.012218002876145965 34-35 0.25383954251303253 36-37 0.449748898975373 38-39 0.44658907064533526 40-41 0.4668821903649111 42-43 0.4555770267841093 44-45 0.4724996629516448 46-47 0.4774149514650368 48-49 0.48457722901312245 50-51 0.4926523458565522 52-53 0.4826111136077656 54-55 0.47046332913895383 56-57 0.46540760381089347 58-59 0.46407345407154416 60-61 0.46042209689016717 62-63 0.4576133605968003 64-65 0.4747466519863383 66-67 0.4487658412726946 68-69 0.47446577835700166 70-71 0.4569111765234586 72-73 0.46414367247887833 74-75 0.47629145694769004 76-77 0.46526716699622506 78-79 0.4642841092935467 80-81 0.4666715351429085 82-83 0.4674439376235844 84-85 0.46821634010426033 86-87 0.4692696162142729 88-89 0.4823302399784289 90-91 0.504378819881359 92-93 0.4933545299298939 94-95 0.4670226271795794 96-97 0.44069072442926477 98-99 0.4321240787344958 100-101 0.44111203487326983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 712064.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.8220930312108 #Duplication Level Percentage of deduplicated Percentage of total 1 70.98476437266741 31.107009481376092 2 13.885884524603883 12.170170469156833 3 5.514950658492049 7.250300424569274 4 2.8609260405676835 5.014870684206823 5 1.71627026463365 3.76052776017383 6 1.0644072794579615 2.798673289410287 7 0.7437421539569014 2.2814636503352705 8 0.5424434090445622 1.901680442825435 9 0.38838407591485186 1.5317822795923328 >10 2.0864403098082605 16.582001972868806 >50 0.1266882631663232 3.823693835288714 >100 0.07934009257988105 6.237158528996548 >500 0.002559357825156076 0.7867772632497404 >1k 0.002239438097011566 1.4437334741528558 >5k 6.39839456289019E-4 1.8064192867022284 >10k+ 3.199197281445095E-4 1.5037371570949243 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10726 1.5063252741326623 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7373 1.0354406345497034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5512 0.7740877224519145 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2065 0.2900020222901312 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1758 0.2468879201869495 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1637 0.2298950656120798 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1381 0.19394324105698363 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1255 0.17624820240877223 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1135 0.15939578464857093 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1067 0.1498460812511235 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 916 0.12864012223620347 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 805 0.11305163580801725 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 786 0.11038333632931872 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 756 0.10617023188926838 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.2131044400503325E-4 0.0 18-19 0.0 0.0 0.0 6.319656660075498E-4 0.0 20-21 0.0 0.0 0.0 0.0016150233686859608 0.0 22-23 0.0 0.0 0.0 0.0034407019593744384 0.0 24-25 0.0 0.0 0.0 0.004774851698723711 0.0 26-27 0.0 0.0 0.0 0.005617472586733776 0.0 28-29 0.0 0.0 0.0 0.007092059140751393 0.0 30-31 0.0 0.0 0.0 0.01306062376415603 0.0 32-33 0.0 0.0 0.0 0.02155705105159087 0.0 34-35 0.0 0.0 0.0 0.031387628078374974 0.0 36-37 0.0 0.0 0.0 0.049433758763257236 0.0 38-39 0.0 0.0 0.0 0.07618697195757684 0.0 40-41 0.0 0.0 0.0 0.1089087497753011 0.0 42-43 0.0 0.0 0.0 0.1379791704116484 0.0 44-45 0.0 0.0 0.0 0.1740714317814129 0.0 46-47 0.0 0.0 0.0 0.21943252291928816 0.0 48-49 0.0 0.0 0.0 0.2721665468272515 0.0 50-51 0.0 0.0 0.0 0.329886077655941 0.0 52-53 0.0 0.0 0.0 0.38676298759662053 0.0 54-55 0.0 0.0 0.0 0.4430781502786266 0.0 56-57 0.0 0.0 0.0 0.5134369944274672 0.0 58-59 0.0 0.0 0.0 0.5936966340104259 0.0 60-61 0.0 0.0 0.0 0.681750516807478 0.0 62-63 0.0 0.0 0.0 0.775913401042603 0.0 64-65 0.0 0.0 0.0 0.86523121517167 0.0 66-67 0.0 0.0 0.0 0.964730698364192 0.0 68-69 0.0 0.0 0.0 1.0881746584576668 0.0 70-71 0.0 0.0 0.0 1.236756808376775 0.0 72-73 0.0 0.0 0.0 1.3906755572532807 0.0 74-75 0.0 0.0 0.0 1.5363085340643536 0.0 76-77 0.0 0.0 0.0 1.684820465576128 0.0 78-79 0.0 0.0 0.0 1.8804489484091318 0.0 80-81 0.0 0.0 0.0 2.0965109877763792 0.0 82-83 0.0 0.0 0.0 2.322965351429085 0.0 84-85 0.0 0.0 0.0 2.53797411468632 0.0 86-87 0.0 0.0 0.0 2.7650604440050333 0.0 88-89 0.0 0.0 0.0 3.0309775525795435 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 40 7.688399E-6 59.183453 2 TAACGCA 90 5.8430487E-7 36.822674 4 ATGGGCG 75 3.1374456E-4 31.562292 5 GTATCAA 10505 0.0 30.746851 1 ATGGGTA 340 0.0 30.633987 5 GGTATCA 7110 0.0 29.1754 1 ACTAGCG 65 0.0059396513 29.132378 8 GTACATG 8360 0.0 27.985497 1 CGAGCAA 35 0.008341643 27.176516 94-95 GTAAGCG 35 0.008341643 27.176516 94-95 TACATGG 8540 0.0 27.110735 2 GAGTACG 70 0.008511055 27.062899 1 GTACCGT 70 0.008531494 27.049593 6 CATGGGT 675 0.0 26.652601 4 CTGGTCG 145 8.115785E-7 26.118681 9 ACATGGG 8580 0.0 25.713335 3 ATCAACG 12650 0.0 25.374584 3 TCAACGC 12665 0.0 25.344532 4 CAACGCA 12725 0.0 25.225029 5 TATCAAC 12810 0.0 25.207254 2 >>END_MODULE