FastQCFastQC Report
Thu 26 May 2016
SRR936289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712064
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109191.5334295793636528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81831.1491944544310624No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60580.8507662232608305No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23760.3336778716519863No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.26542557972317093No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16110.22624370843070288No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13340.18734271076757145No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13060.1834104799568578No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11010.15462093294984722No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10680.14998651806579186No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA10020.1407176882976811No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9070.1273761909041884No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG7520.10560848463059501No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7320.10279974833722812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG301.306665E-463.276622
ATCACGC301.3093925E-463.249963
TATCACG500.00162252837.9659732
GGTATCA70000.031.403551
GTATCAA101850.029.1980481
GTACATG78850.027.0943951
CATATAG1606.3149855E-826.6835753
TACATGG81200.025.949652
ACATGGG80300.025.1660793
GTATAGA1351.3241601E-524.67171
ATGGGAG16100.024.4553435
CATGGGG22550.023.7713264
GTCGGGA4600.023.7287312
CGACCGT601.7256237E-423.720418-19
ATGGGTA3400.023.7187355
AGTACTT73100.023.42671212-13
GGACAGT3650.023.393826
ATCAACG127850.023.3755233
TCAACGC128700.023.2579984
TAGATAG1852.571287E-723.077695
Produced by FastQC (version 0.11.5)