##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936289_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 712064 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37803764830127 34.0 31.0 34.0 31.0 34.0 2 32.47616787255078 34.0 31.0 34.0 31.0 34.0 3 32.540177568308465 34.0 31.0 34.0 31.0 34.0 4 36.06815117742225 37.0 35.0 37.0 35.0 37.0 5 36.05689235799029 37.0 35.0 37.0 35.0 37.0 6 36.136969429714185 37.0 36.0 37.0 35.0 37.0 7 36.08162468542154 37.0 35.0 37.0 35.0 37.0 8 36.07280665782851 37.0 35.0 37.0 35.0 37.0 9 37.775534221643 39.0 38.0 39.0 35.0 39.0 10-11 37.72414839115585 39.0 37.5 39.0 35.0 39.0 12-13 37.65850611742765 39.0 37.0 39.0 35.0 39.0 14-15 38.802526879606326 40.0 38.0 41.0 34.5 41.0 16-17 38.76556882527413 40.0 38.0 41.0 34.5 41.0 18-19 38.94383861563006 40.0 38.5 41.0 35.0 41.0 20-21 38.965864023458565 40.0 38.5 41.0 35.0 41.0 22-23 38.83853136796692 40.0 38.0 41.0 35.0 41.0 24-25 38.93263597092396 40.0 38.0 41.0 35.0 41.0 26-27 38.79346800287614 40.0 38.0 41.0 35.0 41.0 28-29 38.64546796355384 40.0 38.0 41.0 35.0 41.0 30-31 38.37721679511954 40.0 37.5 41.0 34.0 41.0 32-33 38.046601850395476 40.0 36.5 41.0 34.0 41.0 34-35 37.80790069431961 40.0 36.0 41.0 33.0 41.0 36-37 37.58127078464857 40.0 35.5 41.0 33.0 41.0 38-39 37.38501244270178 40.0 35.0 41.0 33.0 41.0 40-41 37.143720929579366 40.0 35.0 41.0 33.0 41.0 42-43 36.983974755078194 40.0 35.0 41.0 32.5 41.0 44-45 36.60192974227036 40.0 35.0 41.0 31.0 41.0 46-47 36.039479597339565 40.0 35.0 41.0 29.5 41.0 48-49 35.53504530491641 39.5 35.0 41.0 26.5 41.0 50-51 35.23263007257775 39.0 35.0 41.0 25.5 41.0 52-53 34.9928222744023 39.0 35.0 41.0 24.0 41.0 54-55 34.788075229192884 39.0 35.0 41.0 24.0 41.0 56-57 34.540959099182096 38.0 35.0 41.0 23.0 41.0 58-59 34.27974521953083 38.0 34.5 40.0 23.0 41.0 60-61 33.99016942297321 37.5 34.0 40.0 22.0 41.0 62-63 33.70610858012763 37.0 34.0 40.0 22.0 41.0 64-65 33.344334217149026 36.5 34.0 40.0 20.0 41.0 66-67 32.99652418883696 36.0 33.0 39.0 19.0 41.0 68-69 32.64619121314938 35.5 33.0 39.0 17.5 41.0 70-71 32.27484186814668 35.0 33.0 38.5 14.0 40.0 72-73 31.885583318353405 35.0 33.0 37.5 10.5 40.0 74-75 31.46772902435736 35.0 32.0 37.0 7.5 39.0 76-77 30.76426486944994 35.0 31.0 36.0 4.5 39.0 78-79 30.80446280109653 35.0 32.0 36.0 2.0 38.5 80-81 30.63817648413626 35.0 32.0 36.0 2.0 37.0 82-83 30.364509369944273 35.0 31.5 35.0 2.0 37.0 84-85 30.07894866191803 35.0 31.0 35.0 2.0 36.5 86-87 29.67704237259572 34.5 31.0 35.0 2.0 36.0 88-89 29.169668035457487 34.0 30.0 35.0 2.0 36.0 90-91 28.935507201599854 34.0 30.0 35.0 2.0 35.5 92-93 28.639512038243755 34.0 29.5 35.0 2.0 35.0 94-95 28.484473305770265 34.0 29.0 35.0 2.0 35.0 96-97 28.220952049254 34.0 29.0 35.0 2.0 35.0 98-99 27.789010538378573 34.0 29.0 35.0 2.0 35.0 100-101 24.80905789367248 30.5 22.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07319497053666169 1101 2 0.22640522571748534 1101 3 0.2008625026354025 1101 4 0.1413301379080707 1101 5 0.2385682669027176 1101 6 0.1878789015741802 1101 7 0.1115940214856721 1101 8 0.19590173042437442 1101 9 0.2283068084880071 1101 10-11 0.16140716797080756 1101 12-13 0.35414727617200725 1101 14-15 0.48578571108274105 1101 16-17 0.43435305694939075 1101 18-19 0.3617253571792105 1101 20-21 0.2634607140422247 1101 22-23 0.33630170265088566 1101 24-25 0.3570367936531653 1101 26-27 0.49901393372561387 1101 28-29 0.39335591491541777 1101 30-31 0.4626299505514595 1101 32-33 0.5675195112624465 1101 34-35 0.661000137638105 1101 36-37 0.670064271131622 1101 38-39 0.6197367245808465 1101 40-41 0.4996862070214476 1101 42-43 0.5251603751467613 1101 44-45 0.4667632825220025 1101 46-47 0.7349835853205349 1101 48-49 0.7781665108875799 1101 50-51 0.7980887473865224 1101 52-53 0.894337064382448 1101 54-55 0.9568954158700507 1101 56-57 0.8106649726727468 1101 58-59 0.7811002060412662 1101 60-61 0.8551409828239045 1101 62-63 0.9258342196607074 1101 64-65 1.0116862466865513 1101 66-67 1.1678824860190602 1101 68-69 0.9194047628211806 1101 70-71 0.6917266767162076 1101 72-73 0.8213654336535399 1101 74-75 0.7655122742888878 1101 76-77 0.4972223680928991 1101 78-79 0.38342159732412995 1101 80-81 0.54374989972899 1101 82-83 0.5243041589774045 1101 84-85 0.8551049840082072 1101 86-87 0.6633047444607847 1101 88-89 0.8328455704240412 1101 90-91 0.9223534157159854 1101 92-93 1.0728752042663885 1101 94-95 1.0582239597330378 1101 96-97 1.1694413414402405 1101 98-99 1.2235715736348922 1101 100-101 0.7831167514874373 1102 1 0.01673565866435922 1102 2 0.18682451773261022 1102 3 0.236357213751603 1102 4 0.17810410972614932 1102 5 0.1478553177294657 1102 6 0.08047828294661485 1102 7 0.15499341813286804 1102 8 0.1989757747975318 1102 9 0.30011724878281143 1102 10-11 0.2000904841200395 1102 12-13 0.2746708183876123 1102 14-15 0.4302619779327088 1102 16-17 0.46116025135882666 1102 18-19 0.27898392058853716 1102 20-21 0.1675257081232573 1102 22-23 0.3460966390697209 1102 24-25 0.3605022536142144 1102 26-27 0.37113559714639166 1102 28-29 0.25691416901096886 1102 30-31 0.2921923288307582 1102 32-33 0.30857423307097065 1102 34-35 0.31508422516245815 1102 36-37 0.058148053160877566 1102 38-39 0.17661609220600383 1102 40-41 0.11896294738880187 1102 42-43 -0.05033509970717631 1102 44-45 0.016406235132073732 1102 46-47 0.4781481326149972 1102 48-49 0.5096363241536963 1102 50-51 0.24386336838167466 1102 52-53 0.3636729944240713 1102 54-55 0.2331679938667719 1102 56-57 0.26780973915993656 1102 58-59 0.11116856056832347 1102 60-61 0.3610885904154486 1102 62-63 0.48852754077423555 1102 64-65 0.520287842898405 1102 66-67 0.6153659701905951 1102 68-69 0.8179517517851664 1102 70-71 0.5658913385713262 1102 72-73 0.3414230958523419 1102 74-75 0.4673872504220853 1102 76-77 0.4929454367911106 1102 78-79 0.45788602623924035 1102 80-81 0.43174776126333825 1102 82-83 0.5077978772345553 1102 84-85 0.4948880824921851 1102 86-87 0.5832459366553842 1102 88-89 0.5095553882725916 1102 90-91 0.7575579064938545 1102 92-93 0.7067202418804754 1102 94-95 0.8089686040221906 1102 96-97 0.36280064185647376 1102 98-99 0.46061667234799586 1102 100-101 0.8458578062092457 1103 1 -0.044340075245685284 1103 2 0.022372597351335344 1103 3 0.1423286182932344 1103 4 -0.12205208395401002 1103 5 -0.0251191706361098 1103 6 0.1529681704070711 1103 7 0.14262608263685195 1103 8 0.06427274303815267 1103 9 0.09741790159229424 1103 10-11 0.22975927341877167 1103 12-13 0.14236676135688953 1103 14-15 0.20725344735478757 1103 16-17 0.2894593422315097 1103 18-19 0.16995672686832108 1103 20-21 0.19423482743119536 1103 22-23 0.13654479478280024 1103 24-25 0.16608117147236356 1103 26-27 0.3089064329828375 1103 28-29 0.24522367279566026 1103 30-31 0.20296568779587432 1103 32-33 0.2508947104629442 1103 34-35 0.3076630228716155 1103 36-37 0.44891660616659834 1103 38-39 0.46092463479881474 1103 40-41 0.528911283325364 1103 42-43 0.6781922742365154 1103 44-45 0.8278604383440538 1103 46-47 0.8187544844913148 1103 48-49 0.8989670943086949 1103 50-51 1.046915152594103 1103 52-53 0.9790600744032503 1103 54-55 1.0175806655796862 1103 56-57 0.8777300403333967 1103 58-59 0.9309611033213443 1103 60-61 0.7692146667501092 1103 62-63 0.851930160022107 1103 64-65 0.9439972425539835 1103 66-67 0.8048689538903773 1103 68-69 0.9178476226650574 1103 70-71 0.7514854753008677 1103 72-73 0.7785771669004404 1103 74-75 0.9178338041487137 1103 76-77 0.7128180788672829 1103 78-79 0.4109558327993774 1103 80-81 0.5540353979328074 1103 82-83 0.620777972552748 1103 84-85 0.4880115555988418 1103 86-87 0.5029503701529201 1103 88-89 0.7211177255708421 1103 90-91 0.7616830216366317 1103 92-93 0.7384155440551687 1103 94-95 0.747688616838083 1103 96-97 0.8108642407190629 1103 98-99 0.435461479909371 1103 100-101 0.9735281173951158 1104 1 0.1194062662177302 1104 2 0.06676934531881784 1104 3 0.1341595126021744 1104 4 0.15849425750940327 1104 5 0.1243702602582033 1104 6 0.2712250809761727 1104 7 0.14334152979132142 1104 8 0.11751501946091736 1104 9 0.023700828421560516 1104 10-11 0.06754138723990621 1104 12-13 0.22243830607234116 1104 14-15 0.3264209270295879 1104 16-17 0.3007503991420748 1104 18-19 0.3731632309333648 1104 20-21 0.34534377052062837 1104 22-23 0.12983259966085114 1104 24-25 0.2644746674073204 1104 26-27 0.2945129370478696 1104 28-29 0.30143830694200346 1104 30-31 0.2527282894218885 1104 32-33 0.0892231657474909 1104 34-35 0.04734432368461938 1104 36-37 -0.09407526375210296 1104 38-39 0.07944170796955063 1104 40-41 0.2069340475530126 1104 42-43 0.2306540628544056 1104 44-45 0.2947287310269857 1104 46-47 0.04469919993847071 1104 48-49 0.13444026504040352 1104 50-51 -0.051862083178257024 1104 52-53 0.003248692289425037 1104 54-55 0.05146684444147809 1104 56-57 0.07037394277241305 1104 58-59 0.1567041927522368 1104 60-61 0.02961141471758566 1104 62-63 0.07169601368064349 1104 64-65 -0.1412320257387023 1104 66-67 -0.16551153391449702 1104 68-69 0.04727851697400354 1104 70-71 -0.04017631331701921 1104 72-73 0.1471820449492185 1104 74-75 0.3154923407340746 1104 76-77 0.11655791626565914 1104 78-79 0.05234119865303555 1104 80-81 0.1891638532173623 1104 82-83 0.13545764734949728 1104 84-85 0.12057740925737548 1104 86-87 0.4320170368195839 1104 88-89 0.5004282946765315 1104 90-91 0.5801155419618347 1104 92-93 0.8160220481202103 1104 94-95 0.6660365843177587 1104 96-97 0.5307861919385779 1104 98-99 0.2602094473890446 1104 100-101 0.38513136942763637 1105 1 -0.03479941201570824 1105 2 0.011364928915028827 1105 3 0.11472481228671683 1105 4 0.08244126066397683 1105 5 0.004721048901735969 1105 6 0.09165915037680605 1105 7 0.18860335944431483 1105 8 0.08749861567542894 1105 9 0.12700934577171807 1105 10-11 0.24193003077303388 1105 12-13 0.18248499376949923 1105 14-15 0.26326509106744567 1105 16-17 0.356033679899177 1105 18-19 0.29559982399329954 1105 20-21 0.2511620670505934 1105 22-23 0.2290199813643241 1105 24-25 0.18444438349564507 1105 26-27 0.2342340726945622 1105 28-29 0.2281966665634485 1105 30-31 0.3423081001474415 1105 32-33 0.23869445912288256 1105 34-35 0.46660867699691977 1105 36-37 0.3674452409797624 1105 38-39 0.4594089003985715 1105 40-41 0.6483939844615279 1105 42-43 0.6889152615925767 1105 44-45 0.6087060180932298 1105 46-47 0.21048847438642326 1105 48-49 0.5800781199299863 1105 50-51 0.49699722281543046 1105 52-53 0.724760364213715 1105 54-55 0.46985170248564145 1105 56-57 0.5272799364632661 1105 58-59 0.4040997763484455 1105 60-61 0.3872290803327232 1105 62-63 0.3958133638383643 1105 64-65 0.3884613496556213 1105 66-67 0.46555218848297386 1105 68-69 0.4776255201285764 1105 70-71 0.2104785762728909 1105 72-73 0.39288046766215245 1105 74-75 0.34609801896751335 1105 76-77 0.20523299513001092 1105 78-79 0.11284088319562002 1105 80-81 0.20413798570947606 1105 82-83 0.3556078050782041 1105 84-85 0.5011376616233072 1105 86-87 0.5069987402896174 1105 88-89 0.6479021117112325 1105 90-91 0.725860652371928 1105 92-93 0.9276233099498619 1105 94-95 0.9113135559265899 1105 96-97 0.7067748354716983 1105 98-99 0.7331558196287915 1105 100-101 0.7627692243172284 1106 1 -0.12361756485316278 1106 2 -0.0684415144096917 1106 3 -0.11273520836313367 1106 4 -0.1075135856278564 1106 5 -0.08353803536774507 1106 6 0.0069210085779829456 1106 7 -0.06740960143039842 1106 8 -0.02060383423440726 1106 9 -0.09345874925566022 1106 10-11 0.03073414742090108 1106 12-13 0.03439486715831208 1106 14-15 -0.034070174026219036 1106 16-17 -0.18795668984736125 1106 18-19 -0.04901836786000047 1106 20-21 -0.10195396703593218 1106 22-23 -0.2132841424205978 1106 24-25 -0.08839773078273083 1106 26-27 -0.18985750035785998 1106 28-29 -0.09407662518469806 1106 30-31 0.060865845982974065 1106 32-33 0.15434171778369432 1106 34-35 0.0706251683301744 1106 36-37 0.05574072418153264 1106 38-39 0.1602477411520482 1106 40-41 0.06702333865406729 1106 42-43 0.18782329362363726 1106 44-45 0.33676674007675444 1106 46-47 0.4732554593653262 1106 48-49 0.2885892846482463 1106 50-51 0.2610453074703045 1106 52-53 -0.06065960333651077 1106 54-55 0.15356639195279342 1106 56-57 0.10628465167135914 1106 58-59 0.46795849139476076 1106 60-61 0.2818696349287464 1106 62-63 -0.07642947651543608 1106 64-65 -0.11360125650792696 1106 66-67 0.21702360937358378 1106 68-69 0.21074759691367007 1106 70-71 0.07609276662265074 1106 72-73 -0.10933439547309831 1106 74-75 0.24223140559078615 1106 76-77 0.3534206613636961 1106 78-79 0.05628931328350362 1106 80-81 0.14043866468040633 1106 82-83 0.27302386152747715 1106 84-85 0.3107831407807531 1106 86-87 0.4257256341046549 1106 88-89 0.3836089884925187 1106 90-91 0.29953756307797264 1106 92-93 0.2601644312272988 1106 94-95 0.3177397517385714 1106 96-97 0.40432706675004226 1106 98-99 0.23779014120503206 1106 100-101 0.37173287772326447 1107 1 -0.029633601986553515 1107 2 0.012820086669719899 1107 3 0.07755852413430375 1107 4 0.08178124268238207 1107 5 0.16343978249872748 1107 6 0.10926396071258182 1107 7 0.068560640170233 1107 8 0.11102539837360581 1107 9 0.10772850552205426 1107 10-11 0.08710514341782982 1107 12-13 0.15186301776920885 1107 14-15 0.28125058106583367 1107 16-17 0.3162377138043553 1107 18-19 0.2543006279679574 1107 20-21 0.17388149704451195 1107 22-23 0.0536059110282352 1107 24-25 0.2545544038158809 1107 26-27 0.24415642963435857 1107 28-29 0.20649662654660972 1107 30-31 0.1405582729474233 1107 32-33 0.14848247381998192 1107 34-35 0.15339506503552514 1107 36-37 0.048086186000787734 1107 38-39 0.16791221867795514 1107 40-41 0.3003917081790419 1107 42-43 0.2255878355067935 1107 44-45 0.2755801313564703 1107 46-47 0.1901344552926716 1107 48-49 0.24141094049344503 1107 50-51 0.2686426944164637 1107 52-53 0.3008157433602676 1107 54-55 0.23760852483687245 1107 56-57 0.3325538439256306 1107 58-59 0.21733417627776674 1107 60-61 0.347635796925168 1107 62-63 0.44569799061638804 1107 64-65 0.3988813185776152 1107 66-67 0.2237985155089035 1107 68-69 0.39541690247647665 1107 70-71 0.42138810183949005 1107 72-73 0.119924054833902 1107 74-75 0.2194330326615841 1107 76-77 0.09935857524424208 1107 78-79 0.03221994659372385 1107 80-81 0.4264172304826026 1107 82-83 0.34443557156695803 1107 84-85 0.49364100069065486 1107 86-87 0.43683285230558155 1107 88-89 0.8801699750719187 1107 90-91 0.553254256953597 1107 92-93 0.5367627400477204 1107 94-95 0.8637117078762415 1107 96-97 0.701867575793603 1107 98-99 0.6462127422819606 1107 100-101 0.055897761519890565 1108 1 -0.21950959986669716 1108 2 -0.17386413357492359 1108 3 -0.1373816809931654 1108 4 0.003797314277960595 1108 5 -0.01798316041137582 1108 6 -0.07737229893648845 1108 7 -0.05443430718101894 1108 8 -0.21579968214025058 1108 9 -0.18835623125820433 1108 10-11 -0.13513226632632325 1108 12-13 -0.02921700688253992 1108 14-15 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-1.3057183150121432 2316 62-63 -1.303244526859899 2316 64-65 -1.2839131068197815 2316 66-67 -1.1941818041847654 2316 68-69 -1.1808295911341062 2316 70-71 -1.2089979349386368 2316 72-73 -1.3379422793751026 2316 74-75 -1.4517509025091684 2316 76-77 -1.241279921572179 2316 78-79 -1.39624258513075 2316 80-81 -1.6647604711069626 2316 82-83 -1.6203910012991507 2316 84-85 -1.4195955637155961 2316 86-87 -1.5736262064236577 2316 88-89 -1.3765662999180641 2316 90-91 -1.393311485065194 2316 92-93 -1.294961735663577 2316 94-95 -1.2566444967633217 2316 96-97 -1.3361759702235858 2316 98-99 -1.4461689309893337 2316 100-101 -1.4191064684102024 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 9.0 5 25.0 6 52.0 7 115.0 8 229.0 9 408.0 10 729.0 11 1258.0 12 2100.0 13 3228.0 14 3992.0 15 5719.0 16 8298.0 17 12129.0 18 10831.0 19 5335.0 20 3830.0 21 3575.0 22 3757.0 23 3896.0 24 4434.0 25 5024.0 26 5960.0 27 7443.0 28 8973.0 29 10849.0 30 13360.0 31 16543.0 32 20191.0 33 26312.0 34 36996.0 35 60317.0 36 90483.0 37 147595.0 38 159804.0 39 28252.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.17179311723676 18.79947285276203 14.354483989873817 19.674250040127394 2 15.802100466754784 21.498671933050257 40.06155038999238 22.63767721020259 3 20.07502134639583 28.264313320150997 29.786086643897182 21.874578689555996 4 12.140762628078376 16.72770986877584 36.8225328060399 34.308994697105874 5 12.191039007729643 40.22166546827252 31.80528716519863 15.78200835879921 6 29.812629201869495 36.54587228114327 17.152109922703577 16.48938859428366 7 27.61128213194319 36.066280559050874 19.248269818443287 17.074167490562647 8 28.436769728563725 32.766155851159446 18.7389054916412 20.058168928635627 9 29.61700071903649 15.66656929714183 19.966604125471868 34.74982585834981 10-11 25.83433745937854 27.463970834000413 24.73183514918645 21.969856557434607 12-13 27.8876617832105 25.51434983372281 26.77083801456049 19.827150368506203 14-15 24.25828296332914 25.26036985439511 27.893279255797232 22.58806792647852 16-17 20.62708286813312 29.29806378404037 28.007510613586796 22.06734273423972 18-19 20.503423849541612 25.867337767391696 33.54171816016538 20.08752022290131 20-21 22.346532296628038 24.484262990590025 33.430936988063564 19.738267724718373 22-23 21.53088982295106 24.918651860860628 33.400415412681184 20.150042903507135 24-25 21.074215060694858 25.192982751804482 33.85829978561763 19.874502401883028 26-27 20.839457139907886 25.25716476591669 33.97531190159028 19.928066192585145 28-29 20.389341456375515 25.424181077553005 34.028510698358325 20.157966767713155 30-31 20.30287007525407 25.44910537044566 33.989041569149556 20.258982985150713 32-33 20.201403830057355 25.763989146860062 33.50548836320943 20.529118659873156 34-35 20.726830470591622 25.449599431500996 33.679844336883406 20.143725761023973 36-37 21.027127496353906 25.275976089211145 33.36921496467661 20.32768144975834 38-39 20.844152834791306 25.377459578717055 33.50258333883379 20.27580424765786 40-41 20.69745673986056 25.57514586231328 33.31390609058104 20.41349130724512 42-43 20.575360126734275 25.89253989587678 32.591592338081654 20.940507639307288 44-45 20.631078528443126 26.14309689832663 32.12405289065847 21.101771682571783 46-47 20.987930891608368 26.5601144971158 31.4022244913151 21.04973011996073 48-49 21.38108735224055 26.748182502584385 30.601445570587444 21.269284574587623 50-51 21.30498960002584 27.15151659852729 30.29728055773636 21.246213243710507 52-53 21.516802408951545 27.453087101182426 29.771590607448477 21.25851988241755 54-55 20.848368923708026 27.22963849279661 29.930041056904578 21.99195152659079 56-57 21.341462986460797 27.533711072427458 29.335286397530485 21.789539543581256 58-59 21.74385650526505 26.87900074994312 29.656177761798965 21.720964982992864 60-61 21.6790440050895 26.953854623385304 30.220109710780363 21.14699166074483 62-63 21.716025525795434 27.08057140931152 30.088166232248785 21.115236832644257 64-65 21.442675396280364 27.20827908923277 30.102491507024 21.246554007462866 66-67 21.47896818263527 27.604190072802442 29.497629426568402 21.41921231799389 68-69 21.53774098957397 27.62483428455869 29.592564713284197 21.24486001258314 70-71 21.48253944179018 27.612061871016852 29.54644398942789 21.358954697765082 72-73 21.640417579222998 27.79800479315897 29.226613337565205 21.334964290052827 74-75 21.11193656749955 28.290855878123317 29.212683129606326 21.384524424770806 76-77 21.29469326307866 27.93805113630368 29.161622095047818 21.60563350556984 78-79 21.37109584766039 27.928131826731406 29.251234961861677 21.449537363746522 80-81 21.00350041955881 28.41996608419966 28.995621842337172 21.580911653904355 82-83 21.135810825993172 28.238192072622688 28.708093654502964 21.91790344688118 84-85 21.006054273592497 28.117964943916164 28.57431757761646 22.301663204874878 86-87 21.27544252220256 28.087813389517258 28.453796246109764 22.18294784217042 88-89 21.143460848280775 27.961316848597157 28.538216702200607 22.357005600921457 90-91 21.006247847482374 28.039037954035955 28.73439358692368 22.22032061155799 92-93 21.12348140359822 28.203022720758547 28.565774564271724 22.107721311371506 94-95 20.752381166385316 28.00437050415282 28.64786825570258 22.59538007375928 96-97 20.983479239681284 27.96296855458971 28.57515025416284 22.478401951566163 98-99 20.99643278467502 28.045334531767878 28.06745407561373 22.890778607943375 100-101 20.86088250990085 28.099177035643063 28.294525742212734 22.74541471224335 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1175.0 1 843.0 2 898.0 3 1356.0 4 1055.5 5 884.0 6 1925.5 7 4044.0 8 5891.5 9 6003.0 10 4362.5 11 2820.0 12 2485.5 13 2518.5 14 2519.0 15 2530.5 16 2513.5 17 2518.0 18 2535.5 19 2572.5 20 2709.0 21 2951.5 22 3168.5 23 3476.5 24 3782.0 25 4142.0 26 4804.0 27 5389.0 28 5890.5 29 6646.0 30 8017.5 31 9569.5 32 11204.5 33 12658.5 34 14078.5 35 14949.5 36 15777.0 37 16887.0 38 17016.0 39 17002.5 40 17081.5 41 17956.0 42 18699.5 43 19520.5 44 20863.5 45 21701.0 46 22332.5 47 24079.5 48 29953.5 49 30467.5 50 25368.0 51 22611.0 52 20808.5 53 20664.5 54 20289.5 55 19219.5 56 18000.0 57 16597.5 58 15592.0 59 14075.5 60 12987.0 61 12005.0 62 10284.5 63 7670.0 64 5278.5 65 4241.0 66 3022.5 67 2230.5 68 1726.0 69 1145.0 70 865.0 71 719.0 72 666.5 73 718.5 74 575.5 75 457.0 76 330.0 77 149.5 78 69.5 79 36.0 80 14.0 81 5.5 82 2.0 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2564376235843969 2 0.01783547546287974 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.8087362933668883E-4 12-13 0.0 14-15 0.0 16-17 7.021840733417221E-4 18-19 0.0 20-21 7.021840733417221E-5 22-23 1.4043681466834442E-4 24-25 0.002598081071364372 26-27 0.0028789547007010605 28-29 0.0021767706273593387 30-31 0.0013341497393492719 32-33 0.0016852417760201332 34-35 0.0018958969980226498 36-37 7.021840733417221E-5 38-39 1.4043681466834442E-4 40-41 7.724024806758943E-4 42-43 0.003230046737371921 44-45 0.004072667625381988 46-47 0.011375381988135898 48-49 0.013481934208161066 50-51 0.00603878303073881 52-53 0.007443151177422255 54-55 4.915288513392055E-4 56-57 0.0028789547007010605 58-59 0.0014043681466834443 60-61 0.0014043681466834443 62-63 0.0 64-65 7.021840733417221E-4 66-67 0.0 68-69 0.0 70-71 2.8087362933668883E-4 72-73 0.0010532761100125832 74-75 0.0 76-77 0.18488506651087544 78-79 0.09914839115585117 80-81 9.128392953442387E-4 82-83 0.0 84-85 7.021840733417221E-4 86-87 0.11635190095272337 88-89 0.5695415018874708 90-91 0.3004645649829229 92-93 0.2913361720294805 94-95 0.0025278626640302 96-97 0.0028789547007010605 98-99 0.0033704835520402664 100-101 5.617472586733777E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 712064.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.65954061387242 #Duplication Level Percentage of deduplicated Percentage of total 1 70.11162988555188 29.90929922607492 2 14.001865075414162 11.946262637091849 3 5.805380402560087 7.429645831859734 4 2.989415479101729 5.101083641699165 5 1.7982748044949137 3.835678852862713 6 1.1282504306466863 2.887838704127482 7 0.7772503001982204 2.320999852391536 8 0.5708690596122572 1.94824094669858 9 0.41253238864658126 1.5838597969205974 >10 2.1750225109800647 16.76496175190251 >50 0.14065665538386446 4.084712321000325 >100 0.08295133164072026 6.371136812287907 >500 0.0022950961323513273 0.6624158403326116 >1k 0.002622967008401517 1.6347796329830162 >5k 6.557417521003792E-4 1.9918631719123145 >10k+ 3.278708760501896E-4 1.5272209798546847 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10919 1.5334295793636528 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8183 1.1491944544310624 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6058 0.8507662232608305 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2376 0.3336778716519863 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1890 0.26542557972317093 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1611 0.22624370843070288 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1334 0.18734271076757145 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1306 0.1834104799568578 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1101 0.15462093294984722 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1068 0.14998651806579186 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1002 0.1407176882976811 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 907 0.1273761909041884 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 752 0.10560848463059501 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 732 0.10279974833722812 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.2131044400503325E-4 0.0 18-19 0.0 0.0 0.0 6.319656660075498E-4 0.0 20-21 0.0 0.0 0.0 0.0016150233686859608 0.0 22-23 0.0 0.0 0.0 0.0035109203667086104 0.0 24-25 0.0 0.0 0.0 0.004985506920726227 0.0 26-27 0.0 0.0 0.0 0.005757909401402122 0.0 28-29 0.0 0.0 0.0 0.00730271436275391 0.0 30-31 0.0 0.0 0.0 0.013481934208161064 0.0 32-33 0.0 0.0 0.0 0.022048579902930073 0.0 34-35 0.0 0.0 0.0 0.03173872011504584 0.0 36-37 0.0 0.0 0.0 0.04992528761459644 0.0 38-39 0.0 0.0 0.0 0.07667850080891606 0.0 40-41 0.0 0.0 0.0 0.10982158907064533 0.0 42-43 0.0 0.0 0.0 0.13917288333632932 0.0 44-45 0.0 0.0 0.0 0.1758268919647672 0.0 46-47 0.0 0.0 0.0 0.22104754628797413 0.0 48-49 0.0 0.0 0.0 0.2737815701959374 0.0 50-51 0.0 0.0 0.0 0.331079790580622 0.0 52-53 0.0 0.0 0.0 0.3883780109653065 0.0 54-55 0.0 0.0 0.0 0.44483361046198094 0.0 56-57 0.0 0.0 0.0 0.5151222362034873 0.0 58-59 0.0 0.0 0.0 0.5956627494157829 0.0 60-61 0.0 0.0 0.0 0.6846294715081791 0.0 62-63 0.0 0.0 0.0 0.7786519189286356 0.0 64-65 0.0 0.0 0.0 0.8684612619090419 0.0 66-67 0.0 0.0 0.0 0.9685927107675714 0.0 68-69 0.0 0.0 0.0 1.0933006021930614 0.0 70-71 0.0 0.0 0.0 1.2418125337048354 0.0 72-73 0.0 0.0 0.0 1.396573903469351 0.0 74-75 0.0 0.0 0.0 1.5431899379831027 0.0 76-77 0.0 0.0 0.0 1.6920529615315476 0.0 78-79 0.0 0.0 0.0 1.887541007549883 0.0 80-81 0.0 0.0 0.0 2.104515886212475 0.0 82-83 0.0 0.0 0.0 2.33061915782851 0.0 84-85 0.0 0.0 0.0 2.544785300197735 0.0 86-87 0.0 0.0 0.0 2.7726440319971237 0.0 88-89 0.0 0.0 0.0 3.03764830127629 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 30 1.306665E-4 63.27662 2 ATCACGC 30 1.3093925E-4 63.24996 3 TATCACG 50 0.001622528 37.965973 2 GGTATCA 7000 0.0 31.40355 1 GTATCAA 10185 0.0 29.198048 1 GTACATG 7885 0.0 27.094395 1 CATATAG 160 6.3149855E-8 26.683575 3 TACATGG 8120 0.0 25.94965 2 ACATGGG 8030 0.0 25.166079 3 GTATAGA 135 1.3241601E-5 24.6717 1 ATGGGAG 1610 0.0 24.455343 5 CATGGGG 2255 0.0 23.771326 4 GTCGGGA 460 0.0 23.728731 2 CGACCGT 60 1.7256237E-4 23.7204 18-19 ATGGGTA 340 0.0 23.718735 5 AGTACTT 7310 0.0 23.426712 12-13 GGACAGT 365 0.0 23.39382 6 ATCAACG 12785 0.0 23.375523 3 TCAACGC 12870 0.0 23.257998 4 TAGATAG 185 2.571287E-7 23.07769 5 >>END_MODULE