FastQCFastQC Report
Thu 26 May 2016
SRR936288_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936288_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810913
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35070.43247549367194754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23960.29546942766979933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17610.21716263026983168No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.10025736423019485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33150.040.570841
GGTATCA18650.036.564771
ATCAACG39700.033.5131033
TCAACGC39750.033.470954
TATCAAC41100.032.601942
CAACGCA40650.032.496945
AACGCAG41100.032.2563366
GTACATG40950.030.1832351
TACATGG41750.028.465322
ACGCAGA47950.027.3520557
CGCAGAG47950.027.3520558
ACATGGG43300.026.8996123
GGGCGTA2850.024.919849
GCAGAGT51900.024.7229779
CATGGGG19600.024.6401254
GTACCTG7500.022.73111
GTACACG1252.3017928E-422.7310981
CGCGGAA1700.022.38550482-83
GAGTACT28400.021.7579412-13
TACCTGG8100.021.627962
Produced by FastQC (version 0.11.5)