##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936288_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 810913 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.73618748250429 33.0 31.0 34.0 30.0 34.0 2 31.828374930479594 34.0 31.0 34.0 30.0 34.0 3 31.893462060664955 34.0 31.0 34.0 30.0 34.0 4 35.37493787866269 37.0 35.0 37.0 33.0 37.0 5 35.364702501994664 37.0 35.0 37.0 33.0 37.0 6 35.45834386672799 37.0 35.0 37.0 33.0 37.0 7 35.40557741705954 37.0 35.0 37.0 33.0 37.0 8 35.39921051950086 37.0 35.0 37.0 33.0 37.0 9 37.06811088242512 39.0 37.0 39.0 34.0 39.0 10-11 36.98446935737866 39.0 37.0 39.0 33.0 39.0 12-13 36.88761433100715 39.0 37.0 39.0 33.0 39.0 14-15 38.12286089876473 40.0 38.0 41.0 33.0 41.0 16-17 38.08071272750591 40.0 38.0 41.0 33.0 41.0 18-19 38.044905557069626 40.0 38.0 41.0 33.0 41.0 20-21 37.97547024156722 40.0 38.0 41.0 33.0 41.0 22-23 37.81362920559913 40.0 38.0 41.0 33.0 41.0 24-25 37.954179424919815 40.0 38.0 41.0 33.0 41.0 26-27 37.90389474579887 40.0 38.0 41.0 33.0 41.0 28-29 37.767436827378525 40.0 38.0 41.0 33.0 41.0 30-31 37.58305576553835 40.0 37.5 41.0 32.5 41.0 32-33 37.38346098780017 40.0 37.0 41.0 32.0 41.0 34-35 37.108279186546525 40.0 37.0 41.0 31.0 41.0 36-37 36.897105484805394 40.0 37.0 41.0 31.0 41.0 38-39 36.7371438119749 40.0 36.0 41.0 30.0 41.0 40-41 36.54296268526957 40.0 36.0 41.0 30.0 41.0 42-43 36.350998812449674 40.0 36.0 41.0 30.0 41.0 44-45 36.160900737810344 40.0 35.0 41.0 29.5 41.0 46-47 35.83717242170245 39.5 35.0 41.0 28.0 41.0 48-49 35.637673215252434 39.0 35.0 41.0 27.0 41.0 50-51 35.31942945790732 39.0 35.0 40.5 26.5 41.0 52-53 35.33871574385908 39.0 35.0 40.5 27.0 41.0 54-55 35.416611276425456 39.0 35.0 41.0 27.0 41.0 56-57 35.264985886278794 39.0 35.0 41.0 26.5 41.0 58-59 35.039287815092365 39.0 35.0 41.0 26.0 41.0 60-61 34.756340692528056 38.0 35.0 41.0 26.0 41.0 62-63 34.46370449111063 38.0 34.0 40.0 25.5 41.0 64-65 34.13519637741656 37.0 34.0 40.0 24.5 41.0 66-67 33.78572608898858 37.0 34.0 40.0 23.5 41.0 68-69 33.41247519770925 36.0 34.0 39.0 22.5 41.0 70-71 33.015359230891605 36.0 33.0 39.0 22.0 41.0 72-73 32.613628095739 35.0 33.0 38.5 20.5 40.0 74-75 32.191715387470666 35.0 33.0 37.0 19.5 39.5 76-77 31.792871121809615 35.0 32.0 37.0 18.0 39.0 78-79 31.401754565532926 35.0 32.0 36.5 16.0 39.0 80-81 31.006747949533427 35.0 32.0 36.0 11.0 37.5 82-83 30.662302861096073 35.0 31.0 35.5 7.5 37.0 84-85 30.350865629235194 35.0 31.0 35.0 4.5 37.0 86-87 30.02434478174601 34.5 31.0 35.0 2.0 36.0 88-89 29.713061080535148 34.0 31.0 35.0 2.0 36.0 90-91 29.37423188430818 34.0 30.0 35.0 2.0 36.0 92-93 29.05492142806935 34.0 30.0 35.0 2.0 35.0 94-95 28.691098798514762 34.0 29.5 35.0 2.0 35.0 96-97 28.288976745964117 34.0 29.0 35.0 2.0 35.0 98-99 27.809910557606056 34.0 29.0 35.0 2.0 35.0 100-101 24.652901729285386 30.5 22.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16135185035350474 1101 2 0.20274927029728218 1101 3 0.0884433561209157 1101 4 0.06047652278604687 1101 5 0.18893387351232604 1101 6 0.10511869072166746 1101 7 0.14814298561868355 1101 8 0.17023359768938207 1101 9 0.232581143682296 1101 10-11 0.36456947668363426 1101 12-13 0.29362174697246957 1101 14-15 0.49192436619682667 1101 16-17 0.4358917621201712 1101 18-19 0.6320232319249968 1101 20-21 0.5790183767944441 1101 22-23 0.6551489230057328 1101 24-25 0.6915262658809596 1101 26-27 0.4331651485599508 1101 28-29 0.4300370003596541 1101 30-31 0.3257541556163659 1101 32-33 0.5552422466045854 1101 34-35 0.40581319040106223 1101 36-37 0.4654965943755087 1101 38-39 0.43407297310461246 1101 40-41 0.36551203202066773 1101 42-43 0.589787877459905 1101 44-45 0.42750236430725863 1101 46-47 0.25858665252832225 1101 48-49 0.4845712931542252 1101 50-51 0.5741436170830667 1101 52-53 0.7850288348908236 1101 54-55 0.599419283425803 1101 56-57 0.7145737760166568 1101 58-59 0.39804783110947284 1101 60-61 0.3595316445400272 1101 62-63 0.5100684962325204 1101 64-65 0.548536991078997 1101 66-67 0.5654008044534393 1101 68-69 0.5785352902225398 1101 70-71 0.7495501877547355 1101 72-73 0.5303596679117959 1101 74-75 0.2247069801535062 1101 76-77 0.060657914150223036 1101 78-79 0.41549924338211497 1101 80-81 0.5735419490149134 1101 82-83 0.5451324939266051 1101 84-85 0.5909100393216953 1101 86-87 0.5449978787394691 1101 88-89 0.32367714015862603 1101 90-91 0.20777369765110976 1101 92-93 0.5815429773813499 1101 94-95 0.9415668589604174 1101 96-97 0.9478550719204613 1101 98-99 0.8626876698732424 1101 100-101 0.5648659083245171 1102 1 0.1725890555159424 1102 2 0.10758833887617669 1102 3 0.18318257830964413 1102 4 0.029370532002637617 1102 5 0.08893853923149209 1102 6 0.1438495433280096 1102 7 -0.054575334148175614 1102 8 0.04887644765420873 1102 9 0.11299158340838744 1102 10-11 0.17060296936922015 1102 12-13 0.17886748681197417 1102 14-15 0.35429013704541035 1102 16-17 0.15592079724945052 1102 18-19 0.21754496699828252 1102 20-21 0.16330907981065224 1102 22-23 0.22927912528260208 1102 24-25 0.23372859299812632 1102 26-27 0.3820916742501126 1102 28-29 0.26248743606604563 1102 30-31 0.23147253280745872 1102 32-33 0.1470564217772079 1102 34-35 0.23406268434997912 1102 36-37 0.23735010514972998 1102 38-39 0.3881174338192537 1102 40-41 0.2844379834684787 1102 42-43 0.5298603037774328 1102 44-45 0.3185596162704485 1102 46-47 0.6696347166137713 1102 48-49 0.527472580892713 1102 50-51 0.4977334931902817 1102 52-53 0.598317520880741 1102 54-55 0.34814428593811186 1102 56-57 0.5263362334868305 1102 58-59 0.44709799835755604 1102 60-61 0.559555511488071 1102 62-63 0.7878035049179317 1102 64-65 0.5359278850307945 1102 66-67 0.7105217183601553 1102 68-69 0.7080816746490797 1102 70-71 0.7251081000966408 1102 72-73 0.7584343768427075 1102 74-75 0.800719620663429 1102 76-77 0.6838077770498607 1102 78-79 0.5628202233266997 1102 80-81 0.6617751847270448 1102 82-83 0.5764924075031281 1102 84-85 0.5252929800168147 1102 86-87 0.6220935044483014 1102 88-89 0.6599283424785618 1102 90-91 0.6184547849790185 1102 92-93 0.7260968269585639 1102 94-95 0.5807847408674753 1102 96-97 1.023609303368847 1102 98-99 0.8499619208458675 1102 100-101 0.9791072982781479 1103 1 0.3372777762794321 1103 2 0.30108089685471384 1103 3 0.37932063893368095 1103 4 0.16825942089152335 1103 5 0.2868814754921871 1103 6 0.2530321571835188 1103 7 0.3296159332206585 1103 8 0.26553569702374347 1103 9 0.2222978188828435 1103 10-11 0.21738138997941547 1103 12-13 0.39047061196649935 1103 14-15 0.28238562238388454 1103 16-17 0.450474028718574 1103 18-19 0.187226764951113 1103 20-21 0.36555404841075756 1103 22-23 0.35231354154131367 1103 24-25 0.4082761378727682 1103 26-27 0.45074494033328705 1103 28-29 0.3653715284361141 1103 30-31 0.34019774667286384 1103 32-33 0.3768628303219401 1103 34-35 0.2975609289229908 1103 36-37 0.3126974818989794 1103 38-39 0.31673212995731603 1103 40-41 0.3644026276854575 1103 42-43 0.22110294829110444 1103 44-45 0.3204448459594147 1103 46-47 0.25858665252832225 1103 48-49 0.4844646196604572 1103 50-51 0.43676949180571256 1103 52-53 0.4337956893028405 1103 54-55 0.2700648714101064 1103 56-57 0.36936768282669163 1103 58-59 0.38491632402635645 1103 60-61 0.38051432076463243 1103 62-63 0.4149050724400638 1103 64-65 0.3246399950045813 1103 66-67 0.45319095635650086 1103 68-69 0.5435783863140173 1103 70-71 0.43455402800051246 1103 72-73 0.3866918065020002 1103 74-75 0.618940211080286 1103 76-77 0.44924811725655545 1103 78-79 0.5359016158006149 1103 80-81 0.5259335687452413 1103 82-83 0.5161279709704694 1103 84-85 0.597982491958664 1103 86-87 0.6400866310862874 1103 88-89 0.5083929619712215 1103 90-91 0.4049169814879434 1103 92-93 0.6605773689157388 1103 94-95 0.5153209125531752 1103 96-97 0.3949130169622812 1103 98-99 0.48110591530761937 1103 100-101 0.5358187159708763 1104 1 0.3538729333539443 1104 2 0.35137010559931525 1104 3 0.2148661901916178 1104 4 0.34780309810497556 1104 5 0.4298724786696084 1104 6 0.3272400915778704 1104 7 0.4078361545833573 1104 8 0.24339590457768878 1104 9 0.3162008335581632 1104 10-11 0.3991989313175921 1104 12-13 0.4975747200692737 1104 14-15 0.5707216329236218 1104 16-17 0.5373863642993442 1104 18-19 0.348638533386854 1104 20-21 0.3000399895544845 1104 22-23 0.4854191361981677 1104 24-25 0.4515174246227147 1104 26-27 0.46185270493639763 1104 28-29 0.6079431613206623 1104 30-31 0.6826600015851838 1104 32-33 0.5118019591026055 1104 34-35 0.4088789951345362 1104 36-37 0.45799405296119744 1104 38-39 0.3890809910878019 1104 40-41 0.6037087732832163 1104 42-43 0.4688290841663729 1104 44-45 0.38644062583668415 1104 46-47 0.6082870519957027 1104 48-49 0.49227607387670247 1104 50-51 0.34428522039335263 1104 52-53 0.09636640630098725 1104 54-55 0.2521663375328771 1104 56-57 0.25187619000231365 1104 58-59 0.13018365717650937 1104 60-61 0.19714153386321698 1104 62-63 0.2504585496380116 1104 64-65 0.279799429691316 1104 66-67 0.43354167039562697 1104 68-69 0.2536915335110521 1104 70-71 0.13859150184491398 1104 72-73 0.0377619845362247 1104 74-75 -0.06543036360320542 1104 76-77 -0.012531238663946453 1104 78-79 -0.022342594740532462 1104 80-81 0.19527479983746687 1104 82-83 0.23898602971401317 1104 84-85 -0.10283969221904599 1104 86-87 0.007903968111346416 1104 88-89 -0.012151074551510987 1104 90-91 0.18505757005063472 1104 92-93 0.2033527865247109 1104 94-95 0.014781067972055695 1104 96-97 0.13591883636357238 1104 98-99 0.0604982951327564 1104 100-101 0.15244716199346087 1105 1 0.33895269961040597 1105 2 0.2622914249442232 1105 3 0.24598393553328535 1105 4 0.20207298878152358 1105 5 0.1497601867785434 1105 6 0.19872015637470497 1105 7 0.4166074743523325 1105 8 0.27373507019126464 1105 9 0.20596310382201466 1105 10-11 0.4083866809847052 1105 12-13 0.39133166410143616 1105 14-15 0.31826588390109123 1105 16-17 0.37140046015454686 1105 18-19 0.4501855153302472 1105 20-21 0.3847465803421457 1105 22-23 0.5674965361829081 1105 24-25 0.3185304094327748 1105 26-27 0.35369627656488234 1105 28-29 0.4151887022978755 1105 30-31 0.48899126266383064 1105 32-33 0.422381483643143 1105 34-35 0.17886126929976598 1105 36-37 0.36622257204827235 1105 38-39 0.38369114994181786 1105 40-41 0.28749853979410744 1105 42-43 0.1183698239019293 1105 44-45 0.242273612851875 1105 46-47 0.3023764614455189 1105 48-49 0.38698037472398283 1105 50-51 0.3495631088986997 1105 52-53 0.5366931044613992 1105 54-55 0.5272144686870845 1105 56-57 0.5503478668323538 1105 58-59 0.4151896364652643 1105 60-61 0.5116419452043601 1105 62-63 0.2988492304288002 1105 64-65 0.22784577129953476 1105 66-67 0.3108831344308456 1105 68-69 0.5113352696759961 1105 70-71 0.38302991517066687 1105 72-73 0.4500911931497953 1105 74-75 0.5094897005151253 1105 76-77 0.480481898808808 1105 78-79 0.39182535118294837 1105 80-81 0.3246386204757705 1105 82-83 0.32692398081106333 1105 84-85 0.4474618165972224 1105 86-87 0.5881901258458342 1105 88-89 0.6100308145644853 1105 90-91 0.6284375220466991 1105 92-93 0.6968561408633 1105 94-95 0.5097813648161775 1105 96-97 0.5047561403404366 1105 98-99 0.7111475356358632 1105 100-101 0.683831218919682 1106 1 0.09214281080548048 1106 2 0.1984892010426833 1106 3 0.24856658445081692 1106 4 0.2187912807454211 1106 5 0.11680359354989633 1106 6 0.17714382705789689 1106 7 0.07507167020182237 1106 8 0.1228751560581145 1106 9 0.20880816979997263 1106 10-11 0.2815287931098709 1106 12-13 0.3980880729737919 1106 14-15 0.23611605704651595 1106 16-17 0.26390745655877623 1106 18-19 0.3004151302581235 1106 20-21 0.28673048653634936 1106 22-23 0.16115700504579422 1106 24-25 0.19335664117361517 1106 26-27 0.12668724975017653 1106 28-29 0.2643586837792 1106 30-31 0.33391855819028393 1106 32-33 0.2921869130189023 1106 34-35 0.16115974379299303 1106 36-37 0.3166622867112423 1106 38-39 0.3099071125390722 1106 40-41 0.3919363776768279 1106 42-43 0.21628792652693107 1106 44-45 0.3971877044819365 1106 46-47 0.3128565351880326 1106 48-49 0.5849781989875922 1106 50-51 0.41213130826923106 1106 52-53 0.5394028097404657 1106 54-55 0.33613000527184766 1106 56-57 0.44931480726533835 1106 58-59 0.3916559748338031 1106 60-61 0.5021160398239815 1106 62-63 0.4209005678423381 1106 64-65 0.3270983809375423 1106 66-67 0.4287640336244891 1106 68-69 0.33816907497250526 1106 70-71 0.42945531315603347 1106 72-73 0.5504491100898079 1106 74-75 0.6529911413176563 1106 76-77 0.7704369723437203 1106 78-79 0.6832693924056805 1106 80-81 0.5782339833337886 1106 82-83 0.6766154746783108 1106 84-85 0.743612756232995 1106 86-87 0.7246918406914986 1106 88-89 0.47533280179241544 1106 90-91 0.6167184411707574 1106 92-93 0.581733112105379 1106 94-95 0.8707210836313131 1106 96-97 0.7343004813497451 1106 98-99 0.8441149245030708 1106 100-101 0.8762443763117886 1107 1 -0.28228577067001126 1107 2 -0.1760097088665944 1107 3 -0.12017441035389709 1107 4 -0.05341943045537789 1107 5 -0.3253602861271645 1107 6 -0.23297192633094 1107 7 -0.2765993514178575 1107 8 -0.0674353890593764 1107 9 -0.12481188714802727 1107 10-11 -0.0559286717123868 1107 12-13 -0.011365871820196105 1107 14-15 0.0520485128974002 1107 16-17 -0.33197454314909436 1107 18-19 -0.04852209890978543 1107 20-21 -0.09935187707608151 1107 22-23 -0.16933073165897383 1107 24-25 0.1081789897465768 1107 26-27 -0.015843239429166545 1107 28-29 0.1612644130219465 1107 30-31 -0.06997042386875307 1107 32-33 0.01841094334307769 1107 34-35 -0.11618854966051373 1107 36-37 0.006164956486784945 1107 38-39 -0.030149799393491605 1107 40-41 0.13984500841829117 1107 42-43 -0.21032254952443452 1107 44-45 0.13153016067543177 1107 46-47 0.3044026967883511 1107 48-49 -0.02768164041111021 1107 50-51 0.008266670668042764 1107 52-53 0.1716905936915154 1107 54-55 0.2873319903369733 1107 56-57 0.28297994654268877 1107 58-59 0.12349455845521362 1107 60-61 0.16294093825349876 1107 62-63 0.27341733396062295 1107 64-65 -0.06838454999091681 1107 66-67 -0.054130828973590894 1107 68-69 -0.025374828904759283 1107 70-71 0.08425270415147423 1107 72-73 -0.3834403588583797 1107 74-75 -0.24599919483422283 1107 76-77 -0.14903065944545446 1107 78-79 -0.1806865639618387 1107 80-81 -0.0658275017502703 1107 82-83 0.16234962237884432 1107 84-85 -0.11169280277887239 1107 86-87 -0.2425919128993108 1107 88-89 -0.055132033089041244 1107 90-91 0.05242021975881528 1107 92-93 -0.31671082621478064 1107 94-95 -0.20359443136216626 1107 96-97 0.06029260817174631 1107 98-99 -0.04764059943474663 1107 100-101 0.06580565311548625 1108 1 -0.137520330097626 1108 2 -0.09295976034383813 1108 3 -0.05370637304069703 1108 4 -0.3027096684986148 1108 5 -0.23372559774646362 1108 6 -0.24237302243219006 1108 7 -0.16843474904055 1108 8 -0.08450607407066002 1108 9 -0.04935353171587309 1108 10-11 -0.20840529896516813 1108 12-13 -0.20045113684998483 1108 14-15 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-0.9122649267475822 2316 84-85 -1.1411839312568368 2316 86-87 -1.0428872060286558 2316 88-89 -0.9502690304331018 2316 90-91 -1.3261871506898082 2316 92-93 -1.233002012393701 2316 94-95 -1.1780320591740256 2316 96-97 -1.4749177033285648 2316 98-99 -1.3584557102969512 2316 100-101 -1.492612322429487 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7404.0 3 1576.0 4 1325.0 5 1112.0 6 1075.0 7 1108.0 8 1340.0 9 1607.0 10 1876.0 11 2246.0 12 2990.0 13 4405.0 14 4517.0 15 3968.0 16 4874.0 17 6377.0 18 5860.0 19 4112.0 20 3799.0 21 3839.0 22 4049.0 23 4350.0 24 5047.0 25 5752.0 26 6771.0 27 7961.0 28 9642.0 29 11587.0 30 14376.0 31 17770.0 32 22216.0 33 29727.0 34 41784.0 35 64101.0 36 102569.0 37 172723.0 38 186278.0 39 38748.0 40 52.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.83160099831845 17.93259746620559 12.935206763693877 23.300594771782084 2 15.556920435709213 19.197943799826348 40.96293117057384 24.2822045938906 3 19.901039309248457 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22-23 23.053333908871718 25.791807211084873 28.514679312067194 22.640179567976222 24-25 22.882301390882084 25.944363483193534 28.686164383477163 22.487170742447223 26-27 22.74711430965728 26.372240642433383 28.41237238000159 22.468272667907744 28-29 22.460468842213928 26.264377279042705 28.824367017376378 22.450786861366982 30-31 22.589994782911134 26.040735227190037 28.8027089394756 22.566561050423225 32-33 22.306109373140366 26.25332768467147 28.800467186269557 22.64009575591861 34-35 22.983749326185542 26.228011394264293 28.41741383829442 22.370825441255743 36-37 22.979422909928314 26.10519326558952 28.145002149253585 22.77038167522858 38-39 22.731757531388137 26.284961131416136 28.4428472948692 22.54043404232653 40-41 22.881575906931392 26.145035921663084 28.50427485446414 22.46911331694138 42-43 22.81504414418265 26.102768326200515 28.332679827226613 22.749507702390222 44-45 22.771848458621395 26.425137862607624 28.193793843902544 22.609219834868444 46-47 22.88504597739605 26.66366800615097 27.666794295605975 22.78449172084701 48-49 23.09783770487029 26.654964071214405 27.549822261409872 22.69737596250543 50-51 22.891273592359475 26.749833148562534 27.46321987022459 22.895673388853393 52-53 23.189617194136684 26.736369500197966 27.360324282994164 22.713689022671183 54-55 22.950788188173927 26.6294539833782 27.45329570763469 22.966462120813187 56-57 22.892436547908883 27.072871559462524 27.33591044535717 22.698781447271422 58-59 23.070423233224304 26.62931162652394 27.329703142035882 22.97056199821588 60-61 23.03277865949072 26.5915744584899 27.798860422156725 22.576786459862653 62-63 22.985098849583615 26.768923147824665 27.64201807266231 22.60395992992941 64-65 22.815312022165333 26.63743175560639 27.648427683866444 22.898828538361837 66-67 22.78939120847811 27.07044242525596 27.28177589485355 22.858390471412378 68-69 22.69368738654457 26.814574336924668 27.638268476584038 22.85346979994672 70-71 22.67112409439142 26.858231994370605 27.51185591532126 22.958787995916712 72-73 22.836029024130816 26.82706383115801 27.406902182632976 22.9300049620782 74-75 22.732480078712396 27.067981761821887 27.272817394547754 22.926720764917963 76-77 22.818409851065635 27.045702486865018 27.21389715407189 22.921990507997457 78-79 22.93839819700831 26.971278758721006 27.249619936984498 22.840703107286185 80-81 22.69115540731416 27.402390309902714 27.18134983372404 22.725104449059092 82-83 22.84934229315518 27.275025105797475 27.111287603714356 22.764344997332994 84-85 22.79598295898398 27.162851724947544 26.935497759603226 23.10566755646525 86-87 22.901712975869653 26.90896137285878 27.24399838924511 22.945327262026456 88-89 22.95751547930547 27.055217422823436 27.074177364679613 22.913089733191487 90-91 22.825034040250852 27.284322396037012 27.164646976103963 22.725996587608172 92-93 22.882434592933187 26.92521719731803 27.224831757284324 22.967516452464462 94-95 22.723738735083103 26.973912041496895 27.21074865273236 23.091600570687643 96-97 23.04724272902938 26.926187880889856 26.992208789575383 23.034360600505376 98-99 22.803053056927435 26.94833645554334 26.910312582248302 23.33829790528092 100-101 22.676257139282747 27.086602859552922 26.91802226849277 23.31911773267156 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 335.0 1 237.0 2 474.0 3 712.0 4 455.0 5 358.0 6 626.5 7 1210.0 8 1818.0 9 1849.0 10 1372.0 11 1021.5 12 988.5 13 1080.0 14 1158.0 15 1224.5 16 1265.0 17 1290.0 18 1316.0 19 1313.5 20 1337.5 21 1460.0 22 1630.0 23 1886.0 24 2282.5 25 2801.0 26 3476.5 27 4333.0 28 5204.0 29 6182.0 30 7427.0 31 8788.5 32 10381.0 33 11868.5 34 13438.5 35 14976.5 36 16525.5 37 18032.5 38 19229.0 39 20307.0 40 21280.5 41 22811.0 42 24574.5 43 26421.5 44 28011.0 45 29429.5 46 30059.0 47 30195.0 48 31950.5 49 32860.0 50 31999.5 51 30892.0 52 29484.5 53 28958.5 54 28814.0 55 27230.0 56 24666.5 57 22321.5 58 20538.5 59 18440.5 60 15895.0 61 13156.0 62 10718.5 63 8674.5 64 6886.5 65 5545.0 66 4271.0 67 3418.5 68 2457.5 69 1558.5 70 1225.5 71 968.0 72 803.5 73 738.5 74 619.0 75 500.0 76 350.5 77 208.0 78 134.5 79 90.0 80 52.5 81 33.5 82 27.0 83 17.5 84 13.0 85 10.5 86 9.0 87 11.5 88 11.5 89 8.0 90 5.0 91 4.5 92 6.5 93 8.5 94 7.5 95 4.5 96 5.0 97 5.5 98 5.5 99 5.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04316122691336802 2 0.011961825744561994 3 0.010605330041570427 4 0.006535842932595728 5 0.009372152129759913 6 0.005179347229604162 7 0.00493271164724206 8 0.008138974217949397 9 0.01331832144755356 10-11 0.0015414723897631435 12-13 0.007953997531177821 14-15 0.007707361948815718 16-17 0.01763444413889036 18-19 0.011345236788656735 20-21 0.002651332510392607 22-23 0.009618787712122016 24-25 0.020779047814007173 26-27 0.007214090784091511 28-29 0.015846336166765115 30-31 0.01430486377700197 32-33 0.012516755804876725 34-35 0.25711759461249234 36-37 0.45084984455792426 38-39 0.44906173658579895 40-41 0.46977912550421563 42-43 0.45979038441855047 44-45 0.4759450150632682 46-47 0.4792129365295661 48-49 0.48950997209318386 50-51 0.5003619377171165 52-53 0.48877006534609757 54-55 0.4736019770308282 56-57 0.46959414881744405 58-59 0.4677443819497282 60-61 0.4651547083349262 62-63 0.4596054077317789 64-65 0.4793362543207471 66-67 0.45109648014028636 68-69 0.4782263942001177 70-71 0.4588655009846926 72-73 0.46755940526295675 74-75 0.4839606714900365 76-77 0.47014907887775875 78-79 0.46984078439980614 80-81 0.47119728010279777 82-83 0.4721221635366556 84-85 0.46879258317476724 86-87 0.47329368255287557 88-89 0.48692029847838175 90-91 0.511460538923411 92-93 0.4985738297449911 94-95 0.47064235004248295 96-97 0.443080823713518 98-99 0.434756872808797 100-101 0.4419709635928885 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 810913.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.63310532512163 #Duplication Level Percentage of deduplicated Percentage of total 1 82.51870487058636 52.509214383226485 2 10.62368488008906 13.520361178312184 3 3.0111839522298145 5.748329567566674 4 1.221433811526731 3.108945055061809 5 0.6314075606700762 2.008921190559854 6 0.4025456928310029 1.5369139482053558 7 0.25674275711100625 1.1436137233294745 8 0.19682822821387208 1.0019833101512319 9 0.13310779053728947 0.7623055849368212 >10 0.9020456185008843 10.845470855763676 >50 0.07053634543829013 3.0475523591386797 >100 0.02984077933018394 3.2849070076752653 >500 0.0013563990547844323 0.5364900098835519 >1k 5.813138806218995E-4 0.9449918261888169 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3507 0.43247549367194754 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2396 0.29546942766979933 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1761 0.21716263026983168 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 813 0.10025736423019485 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.165889559052574E-5 0.0 20-21 0.0 0.0 0.0 1.2331779118105149E-4 0.0 22-23 0.0 0.0 0.0 4.3161226913368024E-4 6.165889559052574E-5 24-25 0.0 0.0 0.0 9.865423294484119E-4 1.2331779118105149E-4 26-27 0.0 0.0 0.0 0.0013564957029915663 1.2331779118105149E-4 28-29 0.0 0.0 0.0 0.0018497668677157724 1.2331779118105149E-4 30-31 0.0 0.0 0.0 0.003144603675116813 1.2331779118105149E-4 32-33 0.0 0.0 0.0 0.005302665020785214 1.2331779118105149E-4 34-35 0.0 0.0 0.0 0.008323950904720975 1.2331779118105149E-4 36-37 0.0 0.0 0.0 0.014551499359364076 1.2331779118105149E-4 38-39 0.0 0.0 0.0 0.02534180608770608 1.2331779118105149E-4 40-41 0.0 0.0 0.0 0.03674870177195334 1.2331779118105149E-4 42-43 0.0 0.0 0.0 0.04741569070911429 1.2331779118105149E-4 44-45 0.0 0.0 0.0 0.060734012156667856 1.2331779118105149E-4 46-47 0.0 0.0 0.0 0.07577878268075613 1.2331779118105149E-4 48-49 0.0 0.0 0.0 0.0963111949124012 1.2331779118105149E-4 50-51 0.0 0.0 0.0 0.11967991634121046 1.2331779118105149E-4 52-53 0.0 0.0 0.0 0.14335693224797236 1.2331779118105149E-4 54-55 0.0 0.0 0.0 0.16882205612685947 1.2331779118105149E-4 56-57 0.0 0.0 0.0 0.196445241351415 1.2331779118105149E-4 58-59 0.0 0.0 0.0 0.2277679603114021 1.2331779118105149E-4 60-61 0.0 0.0 0.0 0.2666747234290239 1.2331779118105149E-4 62-63 0.0 0.0 0.0 0.30256020066270983 1.2331779118105149E-4 64-65 0.0 0.0 0.0 0.339062266852301 1.2331779118105149E-4 66-67 0.0 0.0 0.0 0.3853064385451953 1.2331779118105149E-4 68-69 0.0 0.0 0.0 0.4380247942750949 1.2331779118105149E-4 70-71 0.0 0.0 0.0 0.5031982469142806 1.2331779118105149E-4 72-73 0.0 0.0 0.0 0.5689882885093716 1.2331779118105149E-4 74-75 0.0 0.0 0.0 0.6350249656868246 1.2331779118105149E-4 76-77 0.0 0.0 0.0 0.7116669729058481 1.2331779118105149E-4 78-79 0.0 0.0 0.0 0.7921318316514843 1.2331779118105149E-4 80-81 0.0 0.0 0.0 0.888628003250657 1.2331779118105149E-4 82-83 0.0 0.0 0.0 0.9893786386455761 1.2331779118105149E-4 84-85 0.0 0.0 0.0 1.0891427317110467 1.2331779118105149E-4 86-87 0.0 0.0 0.0 1.19741575236801 1.2331779118105149E-4 88-89 0.0 0.0 0.0 1.331585509172994 1.2331779118105149E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3315 0.0 40.57084 1 GGTATCA 1865 0.0 36.56477 1 ATCAACG 3970 0.0 33.513103 3 TCAACGC 3975 0.0 33.47095 4 TATCAAC 4110 0.0 32.60194 2 CAACGCA 4065 0.0 32.49694 5 AACGCAG 4110 0.0 32.256336 6 GTACATG 4095 0.0 30.183235 1 TACATGG 4175 0.0 28.46532 2 ACGCAGA 4795 0.0 27.352055 7 CGCAGAG 4795 0.0 27.352055 8 ACATGGG 4330 0.0 26.899612 3 GGGCGTA 285 0.0 24.91984 9 GCAGAGT 5190 0.0 24.722977 9 CATGGGG 1960 0.0 24.640125 4 GTACCTG 750 0.0 22.7311 1 GTACACG 125 2.3017928E-4 22.731098 1 CGCGGAA 170 0.0 22.385504 82-83 GAGTACT 2840 0.0 21.75794 12-13 TACCTGG 810 0.0 21.62796 2 >>END_MODULE