FastQCFastQC Report
Thu 26 May 2016
SRR936288_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936288_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810913
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.43740820531918956No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25980.3203796214883718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19050.23492039219990307No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.11517881696310207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19600.038.825951
GTATCAA32100.038.81991
TACCTGG9600.034.107022
GTACCTG9950.033.9385641
ATCAACG40000.030.8351023
TCAACGC40600.030.4962524
TATCAAC41200.030.0614012
CAACGCA41250.030.017565
GTGTACG650.00580606429.2687931
AACGCAG42350.029.2378836
GTACATG39450.028.2115551
TACATGG40250.027.2340372
ATTCGCG1900.026.24277780-81
ACATGGG40100.026.1444763
ACGCAGA47700.025.9585827
CGCAGAG48800.025.373458
ACCTGGG12300.025.0691873
TATAACG950.001233302424.9753912
GTACACG1551.3939043E-624.5480211
TCGCGGA2200.023.88276982-83
Produced by FastQC (version 0.11.5)