##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936287_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 52112 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.03463693583052 33.0 31.0 34.0 30.0 34.0 2 32.06230810561867 33.0 31.0 34.0 30.0 34.0 3 32.12457783236107 33.0 31.0 34.0 30.0 34.0 4 35.69751688670556 37.0 35.0 37.0 33.0 37.0 5 35.731309487258216 37.0 35.0 37.0 35.0 37.0 6 35.87365673933067 37.0 35.0 37.0 35.0 37.0 7 35.80985185753761 37.0 35.0 37.0 35.0 37.0 8 35.85615597175315 37.0 35.0 37.0 35.0 37.0 9 37.41500997850783 39.0 37.0 39.0 34.0 39.0 10-11 37.416122965919556 39.0 37.0 39.0 34.0 39.0 12-13 37.36384134172551 39.0 37.0 39.0 34.0 39.0 14-15 38.20994204789684 40.0 37.5 41.0 33.0 41.0 16-17 38.14973518575376 40.0 37.5 41.0 33.5 41.0 18-19 38.31398718145533 40.0 37.0 41.0 34.0 41.0 20-21 38.32490597175315 40.0 37.0 41.0 34.0 41.0 22-23 38.127293137856924 40.0 36.5 41.0 34.0 41.0 24-25 38.0641119128032 40.0 36.5 41.0 33.5 41.0 26-27 37.70224708320541 40.0 35.5 41.0 33.0 41.0 28-29 37.42662918329751 39.0 35.0 41.0 33.0 41.0 30-31 36.99938593797974 38.5 35.0 41.0 33.0 41.0 32-33 36.34638854774332 38.0 35.0 41.0 32.0 41.0 34-35 35.95801350936445 38.0 35.0 40.0 31.0 41.0 36-37 35.540355388394225 37.5 35.0 40.0 30.5 41.0 38-39 35.172206017807795 37.0 35.0 40.0 30.0 41.0 40-41 34.82409042063249 37.0 35.0 40.0 28.0 41.0 42-43 34.36765044519497 37.0 35.0 40.0 24.5 41.0 44-45 33.72386398526251 37.0 34.5 40.0 20.0 41.0 46-47 32.93017922935216 37.0 33.0 40.0 3.5 41.0 48-49 32.24860876573534 36.0 33.0 40.0 2.0 41.0 50-51 31.652431301811482 36.0 33.0 40.0 2.0 41.0 52-53 31.301715535769112 36.0 32.5 40.0 2.0 41.0 54-55 30.947718375805955 35.0 31.5 40.0 2.0 41.0 56-57 30.379979659195577 35.0 31.0 40.0 2.0 41.0 58-59 29.868101396991094 35.0 29.0 40.0 2.0 41.0 60-61 29.211285308566165 35.0 27.0 39.5 2.0 41.0 62-63 28.723249923242246 35.0 26.0 39.0 2.0 41.0 64-65 28.19699877187596 35.0 22.5 39.0 2.0 40.5 66-67 27.59321269573227 35.0 21.0 38.0 2.0 40.0 68-69 27.06321000921093 35.0 13.0 37.0 2.0 39.5 70-71 26.478325529628492 35.0 4.5 36.5 2.0 39.0 72-73 25.984830749155662 34.0 2.0 36.0 2.0 39.0 74-75 25.57146146760823 34.0 2.0 35.0 2.0 37.5 76-77 24.9299969296899 33.0 2.0 35.0 2.0 37.0 78-79 24.885208781086888 34.0 2.0 35.0 2.0 36.0 80-81 24.672983190052193 33.5 2.0 35.0 2.0 36.0 82-83 24.358094105004604 33.0 2.0 35.0 2.0 36.0 84-85 24.03671898986798 33.0 2.0 35.0 2.0 35.0 86-87 23.75949877187596 33.0 2.0 35.0 2.0 35.0 88-89 23.271492170709244 33.0 2.0 35.0 2.0 35.0 90-91 22.973940743015042 33.0 2.0 35.0 2.0 35.0 92-93 22.609245471292603 32.0 2.0 35.0 2.0 35.0 94-95 22.41196845256371 32.0 2.0 35.0 2.0 35.0 96-97 22.11086697881486 32.0 2.0 35.0 2.0 35.0 98-99 21.75522912189131 32.0 2.0 35.0 2.0 35.0 100-101 19.54106539760516 27.0 2.0 33.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18081664950305765 1101 2 0.211541454247957 1101 3 0.15099485632072884 1101 4 0.1912989421360578 1101 5 0.4995668247332503 1101 6 0.5111969927221622 1101 7 0.2747841513790803 1101 8 0.4672858955566497 1101 9 0.7895412027947941 1101 10-11 -0.3945012562900203 1101 12-13 -0.2368813236444396 1101 14-15 0.3353015653780389 1101 16-17 0.3223852474168325 1101 18-19 0.6165806260714106 1101 20-21 -0.08774269764602849 1101 22-23 0.37456540070781585 1101 24-25 1.0263870185029589 1101 26-27 0.6353037785021414 1101 28-29 0.42001651601800916 1101 30-31 0.7977123317062649 1101 32-33 0.4491170715039843 1101 34-35 0.6508531113368292 1101 36-37 1.4878356893103941 1101 38-39 2.3381667848302214 1101 40-41 1.6916111655574326 1101 42-43 2.2030025235537707 1101 44-45 2.044296116942583 1101 46-47 1.6630316034953339 1101 48-49 2.9876348244178175 1101 50-51 1.603643913998173 1101 52-53 2.2191281047190863 1101 54-55 2.639564028521402 1101 56-57 2.494542367986231 1101 58-59 2.7083301113714917 1101 60-61 3.3258866452145277 1101 62-63 2.191669854377647 1101 64-65 1.6211763630507185 1101 66-67 0.7857315166953533 1101 68-69 1.147523151117003 1101 70-71 1.2012678406510489 1101 72-73 0.9429425237223263 1101 74-75 1.2533632814099995 1101 76-77 1.4208474242652294 1101 78-79 0.4803654376878832 1101 80-81 0.5799638300101115 1101 82-83 0.11654418772240405 1101 84-85 0.5580329008863707 1101 86-87 0.9699077132806799 1101 88-89 1.3154405219777772 1101 90-91 1.5036567651650117 1101 92-93 1.2431469204209158 1101 94-95 0.5239981234259439 1101 96-97 1.2517523701707383 1101 98-99 1.5124973409898494 1101 100-101 1.1867705165985747 1102 1 -0.07017149278943435 1102 2 0.0455335491096136 1102 3 -0.7966336021772946 1102 4 -0.1308354452157161 1102 5 0.009448247658149 1102 6 -0.24769628791024445 1102 7 0.16114778774271343 1102 8 -0.007022404838608054 1102 9 -0.5484034612368305 1102 10-11 -0.05210995194219237 1102 12-13 0.686537648687576 1102 14-15 0.72067705944918 1102 16-17 -0.3223775984329649 1102 18-19 0.3571932742927544 1102 20-21 0.1360715316029797 1102 22-23 0.48671955090544117 1102 24-25 0.8297467022974203 1102 26-27 0.44014567573534435 1102 28-29 0.1695224448717596 1102 30-31 0.005222213129194131 1102 32-33 -0.35621889687546116 1102 34-35 0.6587582496767439 1102 36-37 1.0031518948439881 1102 38-39 0.22650670577884569 1102 40-41 0.045860177415143255 1102 42-43 0.6293859227632623 1102 44-45 0.1238415714880361 1102 46-47 -0.09586167713707283 1102 48-49 0.44860320386445807 1102 50-51 0.044849447595009195 1102 52-53 0.34116367784162094 1102 54-55 -0.16478379756555483 1102 56-57 -0.22482522094657753 1102 58-59 -0.6271441969289064 1102 60-61 -0.0451607856155114 1102 62-63 0.055306218014010256 1102 64-65 1.0594174697700858 1102 66-67 1.5762453506874472 1102 68-69 0.9202504238442764 1102 70-71 0.7709318722716034 1102 72-73 0.6657686106788496 1102 74-75 0.7523751391175075 1102 76-77 0.015709084344276647 1102 78-79 0.3775986392689106 1102 80-81 0.49498359285596294 1102 82-83 0.39124774503465787 1102 84-85 0.18846768349506604 1102 86-87 0.31032273304352387 1102 88-89 0.9676144350212539 1102 90-91 0.029842535915996393 1102 92-93 -0.06861197285971699 1102 94-95 0.9054210483271312 1102 96-97 1.4380171923051215 1102 98-99 0.8657582105550681 1102 100-101 0.9357823743060827 1103 1 -1.0030217343353272 1103 2 -0.1510848083783074 1103 3 0.011095866421740652 1103 4 -0.051125300288184405 1103 5 -0.46204933688291305 1103 6 -0.6100151284899553 1103 7 0.3389255655204906 1103 8 0.05819498646574317 1103 9 0.20570281895641074 1103 10-11 0.2916098548210897 1103 12-13 0.5350883733252516 1103 14-15 -0.012173182096709922 1103 16-17 0.698395348426935 1103 18-19 -0.28266179817102 1103 20-21 0.24887346397013488 1103 22-23 -0.029222478080065173 1103 24-25 -0.42411803200209164 1103 26-27 -0.3088881406897741 1103 28-29 0.5419862129877018 1103 30-31 0.587106271100204 1103 32-33 1.415278687665598 1103 34-35 0.8326712931550091 1103 36-37 -0.7821138056390993 1103 38-39 -1.8077928111293744 1103 40-41 -1.0773282283819654 1103 42-43 -0.37906818351692806 1103 44-45 0.17939712704359323 1103 46-47 1.0695972600609949 1103 48-49 0.9232408850238727 1103 50-51 1.5735934089476693 1103 52-53 1.481501842092829 1103 54-55 0.645119584076955 1103 56-57 0.2824211558650198 1103 58-59 0.5146432426846239 1103 60-61 0.7973512916791776 1103 62-63 0.6553062180140117 1103 64-65 1.4673884842628375 1103 66-67 1.8397719207357568 1103 68-69 1.7924726460664964 1103 70-71 1.2813183457015533 1103 72-73 1.5734980792778828 1103 74-75 0.5639693420160619 1103 76-77 1.2193322727500764 1103 78-79 1.1333957407181856 1103 80-81 1.5254183754646569 1103 82-83 1.6236148947931142 1103 84-85 1.7746995675530393 1103 86-87 2.027230945603911 1103 88-89 2.409884966422222 1103 90-91 1.3670406035488512 1103 92-93 2.322692374966369 1103 94-95 3.135614285042106 1103 96-97 3.5636210570394233 1103 98-99 3.4930528965454037 1103 100-101 3.058487688315747 1104 1 -0.26582366670247737 1104 2 -0.08490123349908174 1104 3 0.09467074564879141 1104 4 0.39090368521906527 1104 5 0.40075259548423503 1104 6 0.2088254512201857 1104 7 0.46549561382966687 1104 8 0.40602107342226645 1104 9 -0.15709911341073735 1104 10-11 -0.1064577780291458 1104 12-13 -0.06346235131243105 1104 14-15 0.438068363797008 1104 16-17 0.7645789233061606 1104 18-19 -0.12106759527246425 1104 20-21 0.625201966385589 1104 22-23 0.3997630291663157 1104 24-25 0.5253988762104669 1104 26-27 0.5162326322570863 1104 28-29 0.7890876622630643 1104 30-31 1.2117439522596243 1104 32-33 1.4698680596462737 1104 34-35 1.582671293155009 1104 36-37 1.3835866774526835 1104 38-39 1.5308545318657991 1104 40-41 1.5784688730673153 1104 42-43 1.9554728792850042 1104 44-45 2.1781893975749895 1104 46-47 2.9258774532977085 1104 48-49 3.850777116907935 1104 50-51 3.653545099768923 1104 52-53 3.862902808276406 1104 54-55 4.150433593738793 1104 56-57 4.525174779053419 1104 58-59 4.568507976984133 1104 60-61 3.759187040471449 1104 62-63 4.816175783231401 1104 64-65 4.722460948030953 1104 66-67 4.250158394165705 1104 68-69 3.5615547716703624 1104 70-71 4.010062307054213 1104 72-73 3.578812088939717 1104 74-75 2.991505573900117 1104 76-77 2.472230823474714 1104 78-79 1.9754247262254339 1104 80-81 1.386287940682049 1104 82-83 2.543421658078138 1104 84-85 3.188467683495066 1104 86-87 2.940757515652219 1104 88-89 3.424136174151691 1104 90-91 3.5950599272203476 1104 92-93 2.931388027140283 1104 94-95 3.1010732222401707 1104 96-97 3.41627806187034 1104 98-99 3.257062558381154 1104 100-101 2.5444780264799967 1105 1 0.2262711949576044 1105 2 0.3024505451570434 1105 3 0.12826758359345547 1105 4 0.1912989421360578 1105 5 0.13593046109688345 1105 6 0.19301517454034212 1105 7 0.41114778774271343 1105 8 0.17183135010211004 1105 9 0.6077230209766142 1105 10-11 0.3554987437099797 1105 12-13 0.4108459490828267 1105 14-15 0.34666520174167204 1105 16-17 -0.43897838894680064 1105 18-19 0.6279442624350509 1105 20-21 0.2531663932630579 1105 22-23 -0.12543459929218415 1105 24-25 -0.12134025422431449 1105 26-27 -0.30787803967967164 1105 28-29 0.5677437887452754 1105 30-31 0.37725778625172524 1105 32-33 0.017298889685804397 1105 34-35 -0.678692343208624 1105 36-37 -0.8644370379623325 1105 38-39 -1.3550150333515916 1105 40-41 -1.7742979253516538 1105 42-43 -1.717452021900769 1105 44-45 -1.9897947921483272 1105 46-47 -0.8710593055955727 1105 48-49 -0.023728811945815664 1105 50-51 -0.3395379041836435 1105 52-53 -0.5422355316445469 1105 54-55 -0.30361778966041797 1105 56-57 0.4718150952589575 1105 58-59 1.2196937477351248 1105 60-61 1.2917957361236212 1105 62-63 1.1121243998321901 1105 64-65 -0.4356418187674649 1105 66-67 -0.18017757421374014 1105 68-69 -0.07974957615572364 1105 70-71 0.13308602246922874 1105 72-73 -0.06842111264131034 1105 74-75 0.7988178268645463 1105 76-77 0.7163019697197726 1105 78-79 0.8780927104151566 1105 80-81 -0.32912707908079497 1105 82-83 -0.3493649031866859 1105 84-85 0.012578355431823951 1105 86-87 0.04945316782613318 1105 88-89 0.7586223401595973 1105 90-91 0.7309294924377383 1105 92-93 0.4931469204209158 1105 94-95 -0.4191836947558727 1105 96-97 -0.9187021752838085 1105 98-99 -0.11250265901015055 1105 100-101 0.5844977893258481 1106 1 0.4192004878868971 1106 2 0.1822485249550283 1106 3 0.1444291997550735 1106 4 0.12665247748959274 1106 5 0.16017288533930696 1106 6 -0.03223735071217959 1106 7 0.42781445440937915 1106 8 0.39152831979907887 1106 9 -0.2831860699324764 1106 10-11 0.30272096593220255 1106 12-13 -0.16491162667474413 1106 14-15 0.032271262347734364 1106 16-17 0.3539509039824935 1106 18-19 0.061782646273428554 1106 20-21 0.22665124174790918 1106 22-23 0.4374441885866034 1106 24-25 0.6203264124423526 1106 26-27 0.32444519264355876 1106 28-29 -0.41356934256785394 1106 30-31 0.04266182665576679 1106 32-33 0.45972313211004234 1106 34-35 0.19933795982167624 1106 36-37 1.017886194360898 1106 38-39 1.1810960777595199 1106 40-41 0.7448939938402575 1106 42-43 0.9209318164830691 1106 44-45 1.7905082381547004 1106 46-47 1.869597260060992 1106 48-49 1.0010186628016555 1106 50-51 1.3958156311698922 1106 52-53 1.3481685087594961 1106 54-55 0.5895640285214014 1106 56-57 0.8379767114205734 1106 58-59 1.7813099093512896 1106 60-61 2.341795736123622 1106 62-63 1.6219728846806802 1106 64-65 1.756277373151729 1106 66-67 1.484216365180199 1106 68-69 1.60358375717761 1106 70-71 0.14798501236821693 1106 72-73 -0.059835254055453646 1106 74-75 1.2195248975716133 1106 76-77 0.8304433838611871 1106 78-79 0.6445068518292985 1106 80-81 0.8587517087979926 1106 82-83 1.1791704503486677 1106 84-85 2.2413662342197007 1106 86-87 2.6383420567150218 1106 88-89 2.3987738553111093 1106 90-91 2.4781517146599583 1106 92-93 1.989359041633037 1106 94-95 1.846725396153218 1106 96-97 1.6858432792616433 1106 98-99 1.4041640076565152 1106 100-101 1.3918210216490792 1107 1 0.2680893767757837 1107 2 0.43335963606613603 1107 3 0.13554031086618323 1107 4 -0.3777919669548453 1107 5 0.14683955200597865 1107 6 0.3966515381767124 1107 7 0.12114778774271429 1107 8 0.35819498646574033 1107 9 0.25681393006752273 1107 10-11 0.040498743709981966 1107 12-13 -0.12824496000807528 1107 14-15 0.6989379290143987 1107 16-17 1.0428397928713764 1107 18-19 0.6706715351623131 1107 20-21 0.8199845750812429 1107 22-23 0.7641108552532714 1107 24-25 1.0836597457756838 1107 26-27 1.2966674148657802 1107 28-29 0.19865287965436806 1107 30-31 0.14043960443353853 1107 32-33 -0.4836102012232857 1107 34-35 0.2726712931550068 1107 36-37 -0.8198915834168758 1107 38-39 -0.45001503335159043 1107 40-41 -1.8706615617152877 1107 42-43 -2.410179294628044 1107 44-45 -2.4661584285119638 1107 46-47 -2.351513851050118 1107 48-49 -2.3100924483094545 1107 50-51 -1.6186288132745545 1107 52-53 -1.1940537134627256 1107 54-55 -0.9804359714785988 1107 56-57 -1.7009121774683145 1107 58-59 -1.2775789795376014 1107 60-61 -1.3982042638763765 1107 62-63 -1.1646937819859886 1107 64-65 -1.3092781824038262 1107 66-67 -1.0024503014864656 1107 68-69 -1.409749576155722 1107 70-71 -1.4464594320762245 1107 72-73 -0.9420574762776752 1107 74-75 -1.559363991317273 1107 76-77 -2.00733439391659 1107 78-79 -1.4032709259484797 1107 80-81 -0.6645816245353409 1107 82-83 -0.1952739940957784 1107 84-85 -0.13196709911363058 1107 86-87 -0.14054683217386454 1107 88-89 -0.5745594780222234 1107 90-91 -1.2840705075622623 1107 92-93 -1.167307625033633 1107 94-95 -1.233274603846784 1107 96-97 -1.404156720738353 1107 98-99 -2.212502659010152 1107 100-101 -1.5059567561287004 1108 1 -0.05417477416761329 1108 2 0.30845397568877786 1108 3 -0.2010634627187251 1108 4 0.15013256134703568 1108 5 0.4155187972889962 1108 6 0.49061380232765117 1108 7 0.538506278308752 1108 8 0.5298930996732878 1108 9 0.535681854595822 1108 10-11 0.5559704418231846 1108 12-13 0.25647202112399725 1108 14-15 0.2366737780710011 1108 16-17 0.3937831890977961 1108 18-19 0.41482247855854837 1108 20-21 0.6165883486661485 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86-87 -3.2156983473253824 2316 88-89 -3.3133473568101017 2316 90-91 -3.6440705075622617 2316 92-93 -3.7757924735184822 2316 94-95 -3.7941836947558762 2316 96-97 -3.729308235889871 2316 98-99 -3.2488662953737837 2316 100-101 -2.442017362189304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 4.0 5 9.0 6 15.0 7 42.0 8 77.0 9 91.0 10 185.0 11 266.0 12 454.0 13 661.0 14 767.0 15 1031.0 16 1187.0 17 1559.0 18 1476.0 19 1057.0 20 972.0 21 956.0 22 957.0 23 841.0 24 832.0 25 789.0 26 774.0 27 741.0 28 829.0 29 950.0 30 1185.0 31 1282.0 32 1540.0 33 1878.0 34 2673.0 35 5836.0 36 5091.0 37 7413.0 38 6807.0 39 881.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.55014811680068 21.18070249682607 14.569307121148 13.699842265225254 2 13.945721853287782 28.53825189052244 35.25392499328241 22.262101262907375 3 25.285922628185446 32.564476512127726 30.06409272336506 12.085508136321769 4 10.855465151980349 22.21369358305189 35.644381332514584 31.286459932453177 5 8.015428308259134 45.12780165796745 31.71246545901136 15.144304574762051 6 23.48595333128646 38.908504758980655 15.885016886705557 21.720525023027328 7 17.665796745471294 42.23787227509979 21.44611605772183 18.650214921707093 8 26.128338962235187 30.18690512741787 20.45785999385938 23.226895916487567 9 23.629874117285844 20.83781086889776 23.267193736567393 32.265121277249 10-11 23.033082591341724 30.039146453791833 23.411114522566777 23.516656432299662 12-13 29.08735032238256 29.970064476512125 25.69369818851704 15.248887012588272 14-15 24.25832821614983 29.62369511820694 31.662572919864907 14.455403745778323 16-17 15.197075529628492 34.171591955787534 32.064591648756526 18.566740865827448 18-19 14.376727049431992 32.59805802886092 36.897451642615906 16.12776327909119 20-21 17.573687442431684 28.70643997543752 35.59544826527479 18.124424316856004 22-23 15.067546822229044 30.957361068467915 36.0080211851397 17.967070924163338 24-25 17.955901824950587 31.542284738346986 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50-51 13.217486422690897 32.488629603331475 36.98209522347387 17.31178875050375 52-53 14.828532499184401 33.582490548657624 32.59321806213898 18.995758890018998 54-55 13.740729013749364 31.37982978805062 33.15870777083753 21.720733427362482 56-57 16.680579142799573 30.177599953944906 32.466921888642624 20.674899014612897 58-59 17.89257546391357 30.045479841108403 33.038130145266834 19.023814549711194 60-61 15.872696045978335 32.198267177110615 33.319900597756735 18.609136179154316 62-63 13.543905434448881 32.2267424009825 33.69185600245625 20.537496162112372 64-65 13.050987315537988 34.458175817006 31.78023833739518 20.71059853006083 66-67 15.71902824685293 31.00725360761437 29.99980810561867 23.273910039914032 68-69 15.3045363831747 30.448840957936753 31.48411114522567 22.76251151366288 70-71 17.57560638624501 30.2272029474977 30.225284003684372 21.97190666257292 72-73 17.087399134548036 27.918981414850848 33.52618903696881 21.467430413632307 74-75 15.547282775560332 31.501381639545595 31.279743629106537 21.671591955787534 76-77 15.959594017915496 28.927184652646957 31.389604398139248 23.723616931298295 78-79 16.606484326982176 29.27358635525507 32.43988168408113 21.680047633681625 80-81 17.63481097677989 28.325657263481098 31.452696219535596 22.586835540203413 82-83 15.684487258213078 28.425314706785386 32.45605618667485 23.434141848326682 84-85 16.06155971753147 29.94415873503224 30.74915566472214 23.245125882714156 86-87 16.28990575372998 29.556437279635695 30.21260651942088 23.941050447213442 88-89 16.309654759837127 28.984542349331328 28.99129662864972 25.714506262181825 90-91 17.437753245878287 27.58587501563989 29.396252201615 25.580119536866825 92-93 17.491580871740595 28.919465024535747 29.732512267872607 23.856441835851054 94-95 17.063575828519888 28.034388133023736 29.706779758592234 25.195256279864136 96-97 15.455470054307153 28.276179693346894 31.263073055592873 25.005277196753084 98-99 17.6392700197655 28.589933027575753 28.122661242348062 25.648135710310683 100-101 16.222906652082553 27.770261842395634 28.106080231810637 27.900751273711176 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2055.0 1 1167.5 2 460.5 3 576.0 4 327.5 5 228.5 6 449.0 7 713.5 8 888.0 9 797.0 10 451.0 11 217.5 12 193.5 13 175.0 14 149.0 15 142.5 16 142.5 17 156.5 18 175.0 19 181.0 20 185.5 21 214.5 22 257.5 23 297.0 24 322.0 25 351.0 26 410.0 27 481.5 28 540.0 29 618.5 30 725.5 31 815.0 32 883.5 33 1018.0 34 1123.5 35 1098.5 36 1180.0 37 1247.5 38 1223.0 39 1108.5 40 911.0 41 855.0 42 873.5 43 911.5 44 916.5 45 878.5 46 915.5 47 1965.0 48 4100.5 49 4296.0 50 3697.5 51 3308.0 52 2035.0 53 1108.0 54 565.5 55 326.5 56 252.0 57 247.5 58 219.0 59 187.0 60 162.5 61 139.0 62 123.0 63 105.5 64 70.5 65 42.5 66 32.5 67 23.0 68 20.0 69 22.5 70 21.0 71 16.5 72 10.5 73 7.0 74 7.0 75 5.5 76 6.5 77 4.5 78 1.0 79 0.5 80 0.5 81 0.5 82 0.5 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24178692047896838 2 0.019189438133251458 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001918943813325146 26-27 9.59471906662573E-4 28-29 0.002878415719987719 30-31 9.59471906662573E-4 32-33 0.001918943813325146 34-35 9.59471906662573E-4 36-37 9.59471906662573E-4 38-39 9.59471906662573E-4 40-41 0.0 42-43 0.0047973595333128645 44-45 0.002878415719987719 46-47 0.017270494319926312 48-49 0.013432606693276021 50-51 0.005756831439975438 52-53 0.005756831439975438 54-55 9.59471906662573E-4 56-57 9.59471906662573E-4 58-59 0.001918943813325146 60-61 9.59471906662573E-4 62-63 0.0 64-65 0.001918943813325146 66-67 0.0 68-69 0.0 70-71 0.0 72-73 9.59471906662573E-4 74-75 0.0 76-77 0.1727049431992631 78-79 0.092109303039607 80-81 0.003837887626650292 82-83 0.0 84-85 0.0 86-87 0.12952870739944736 88-89 0.5622505373042678 90-91 0.30990942585201103 92-93 0.28208474055879645 94-95 0.001918943813325146 96-97 0.001918943813325146 98-99 0.001918943813325146 100-101 9.59471906662573E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 52112.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.62020264046669 #Duplication Level Percentage of deduplicated Percentage of total 1 84.35374149659864 34.2646607307338 2 7.4782690854119425 6.075376112987412 3 2.7825018896447467 3.390773718145533 4 1.436130007558579 2.3334356770033775 5 0.8550642479213907 1.7366441510592572 6 0.6235827664399093 1.5198035001535155 7 0.3448601662887377 0.9805802886091496 8 0.2928949357520786 0.9517961314092724 9 0.24092970521541948 0.8807952103162419 >10 1.2566137566137565 9.648449493398834 >50 0.10865457294028723 2.8304421246545903 >100 0.18424036281179137 17.199493398833283 >500 0.02362055933484505 6.626112987411728 >1k 0.01889644746787604 11.561636475284004 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2140 4.106539760515812 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1418 2.7210623272950567 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1344 2.579060485108996 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1123 2.154973902364139 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 860 1.6502916794596252 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 820 1.5735339269266198 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 713 1.3682069389008291 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 533 1.0227970525023027 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 527 1.0112833896223519 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 464 0.8903899293828677 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 446 0.8558489407430151 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 431 0.827064783543138 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 420 0.8059564015965612 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 373 0.7157660423702794 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 369 0.7080902671169789 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 362 0.6946576604237028 No Hit CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT 356 0.6831439975437519 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 329 0.631332514583973 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 324 0.6217377955173473 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 314 0.6025483573840958 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 312 0.5987104697574455 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 268 0.514276941971139 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 242 0.4643844028246853 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 234 0.44903285231808415 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 216 0.41449186367823154 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 213 0.4087350322382561 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 211 0.40489714461160575 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 205 0.39338348173165494 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 198 0.3799508750383789 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 188 0.3607614369051274 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 172 0.33005833589192507 No Hit ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT 168 0.3223825606386245 RNA PCR Primer, Index 23 (95% over 23bp) GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 162 0.31086889775867366 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 160 0.30703101013202333 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 155 0.2974362910653976 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 148 0.2840036843721216 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 146 0.2801657967454713 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 139 0.26673319005219526 No Hit TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG 132 0.25330058335891925 RNA PCR Primer, Index 23 (96% over 30bp) GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 131 0.2513816395455941 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 128 0.24562480810561868 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 127 0.24370586429229352 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 124 0.2379490328523181 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 124 0.2379490328523181 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 122 0.23411114522566778 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 120 0.23027325759901748 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 116 0.2225974823457169 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 114 0.2187595947190666 No Hit ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT 95 0.18229966226588887 RNA PCR Primer, Index 23 (96% over 25bp) GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 83 0.1592723365059871 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 80 0.15351550506601166 No Hit GTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.14775867362603623 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 72 0.1381639545594105 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 67 0.12856923549278476 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 66 0.12665029167945963 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 65 0.12473134786613449 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 63 0.12089346023948419 No Hit GGTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.11705557261283389 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 61 0.11705557261283389 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.11705557261283389 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.11705557261283389 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 60 0.11513662879950874 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 60 0.11513662879950874 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 59 0.1132176849861836 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 58 0.11129874117285846 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 57 0.1093797973595333 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 57 0.1093797973595333 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 57 0.1093797973595333 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 53 0.10170402210623274 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.001918943813325146 0.0 2 0.0 0.0 0.0 0.001918943813325146 0.0 3 0.0 0.0 0.0 0.001918943813325146 0.0 4 0.0 0.0 0.0 0.001918943813325146 0.0 5 0.0 0.0 0.0 0.001918943813325146 0.0 6 0.0 0.0 0.0 0.001918943813325146 0.0 7 0.0 0.0 0.0 0.001918943813325146 0.0 8 0.0 0.0 0.0 0.001918943813325146 0.0 9 0.0 0.0 0.0 0.001918943813325146 0.0 10-11 0.0 0.0 0.0 0.001918943813325146 0.0 12-13 0.0 0.0 0.0 0.001918943813325146 0.0 14-15 0.0 0.0 0.0 0.001918943813325146 0.0 16-17 0.0 0.0 0.0 0.002878415719987719 0.0 18-19 0.0 0.0 0.0 0.005756831439975438 0.0 20-21 0.0 0.0 0.0 0.008635247159963156 0.0 22-23 0.0 0.0 0.0 0.020148910039914033 0.0 24-25 0.0 0.0 0.0 0.04221676389315321 0.0 26-27 0.0 0.0 0.0 0.06812250537304268 0.0 28-29 0.0 0.0 0.0 0.18229966226588884 0.0 30-31 0.0 0.0 0.0 0.4154513355848941 0.0 32-33 0.0 0.0 0.0 0.7090497390236414 0.0 34-35 0.0 0.0 0.0 1.123541602701873 0.0 36-37 0.0 0.0 0.0 1.6637242861529014 0.0 38-39 0.0 0.0 0.0 2.3084894074301503 0.0 40-41 0.0 0.0 0.0 3.2065551120663187 0.0 42-43 0.0 0.0 0.0 3.9175237949032855 0.0 44-45 0.0 0.0 0.0 4.464422781700952 0.0 46-47 0.0 0.0 0.0 5.188824071231194 0.0 48-49 0.0 0.0 0.0 6.012050967147681 0.0 50-51 0.0 0.0 0.0 6.695194964691434 0.0 52-53 0.0 0.0 0.0 7.202755603315935 0.0 54-55 0.0 0.0 0.0 7.692086275713847 0.0 56-57 0.0 0.0 0.0 8.146875959471906 0.0 58-59 0.0 0.0 0.0 8.46542063248388 0.0 60-61 0.0 0.0 0.0 8.838655204175621 0.0 62-63 0.0 0.0 0.0 9.188862450107461 0.0 64-65 0.0 0.0 0.0 9.526596561252687 0.0 66-67 0.0 0.0 0.0 9.895993245317776 0.0 68-69 0.0 0.0 0.0 10.360377648142462 0.0 70-71 0.0 0.0 0.0 10.84107307338041 0.0 72-73 0.0 0.0 0.0 11.255564937058644 0.0 74-75 0.0 0.0 0.0 11.682529935523487 0.0 76-77 0.0 0.0 0.0 12.072075529628492 0.0 78-79 0.0 0.0 0.0 12.63048817930611 0.0 80-81 0.0 0.0 0.0 13.16779244703715 0.0 82-83 0.0 0.0 0.0 13.677272029474977 0.0 84-85 0.0 0.0 0.0 14.165643229966227 0.0 86-87 0.0 0.0 0.0 14.637703408044214 0.0 88-89 0.0 0.0 0.0 15.141426159042062 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTGCAT 25 0.0050291806 56.900387 3 GAGTAAT 55 4.9489106E-5 43.10635 6 TAATGGG 45 9.623063E-4 42.14843 9 AGTAATG 60 8.266972E-5 39.514153 7 GTAATGG 60 8.266972E-5 39.514153 8 ATGGGCA 50 0.0016155171 37.93359 5 CATGGGG 160 1.8517312E-9 29.635616 4 GTTGAGA 35 0.008389397 27.12145 24-25 GAGTACT 995 0.0 24.54246 12-13 CATGGGC 240 2.2009772E-10 23.708492 4 GTACTTT 1030 0.0 22.327415 14-15 ACTTTTT 1065 0.0 21.816265 16-17 GGCTTGA 185 6.4538E-6 20.504642 8 TGGGCTT 185 6.4538E-6 20.504642 6 GGGCTTG 185 8.2628685E-7 20.504642 7 GAGTAAA 70 4.8682227E-4 20.321566 1 AGTAAAA 70 4.8682227E-4 20.321566 2 AGTACTT 935 0.0 19.524641 12-13 GCTTGAT 195 1.4792531E-6 19.453123 9 ATGGGCT 200 1.276585E-7 18.966795 5 >>END_MODULE