FastQCFastQC Report
Thu 26 May 2016
SRR936285_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936285_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701236
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52330.30759988620038603No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39020.2293626516250538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25510.14994980120336038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28200.050.5428921
GTATCAA53700.042.767131
ATATGCG801.10291185E-535.5065
ATCAACG65050.034.7145583
CAACGCA66750.034.1141365
TCAACGC66550.034.0743834
TATCAAC67400.033.5734332
AACGCAG69450.032.989476
ACGCAGA83850.027.2127087
TACATGG80550.026.9170682
GTACATG83350.026.474211
CGCAGAG86700.026.3189478
TACCTGG14050.025.9443112
ACATGGG82000.025.5181353
GTACCGT1701.1614429E-725.0608526
GTACCTG15950.024.047341
AGTACAT58000.023.6699732
GAGTACA61650.023.2729911
GCAGAGT96200.022.8340769
CATGGGG48700.022.4555424
Produced by FastQC (version 0.11.5)