##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936285_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1701236 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01712460822602 33.0 31.0 34.0 30.0 34.0 2 32.11607384278254 34.0 31.0 34.0 30.0 34.0 3 32.18329732030124 34.0 31.0 34.0 30.0 34.0 4 35.68813556731694 37.0 35.0 37.0 35.0 37.0 5 35.67995386883419 37.0 35.0 37.0 35.0 37.0 6 35.771594299673886 37.0 35.0 37.0 35.0 37.0 7 35.72458083416998 37.0 35.0 37.0 35.0 37.0 8 35.729839951658676 37.0 35.0 37.0 35.0 37.0 9 37.43593069979709 39.0 38.0 39.0 35.0 39.0 10-11 37.36630014883296 39.0 37.5 39.0 35.0 39.0 12-13 37.2824084959406 39.0 37.0 39.0 35.0 39.0 14-15 38.567260803321815 40.0 38.0 41.0 34.5 41.0 16-17 38.519054381637815 40.0 38.0 41.0 34.0 41.0 18-19 38.48004156977633 40.0 38.0 41.0 34.0 41.0 20-21 38.407924297393194 40.0 38.0 41.0 34.0 41.0 22-23 38.25171757475153 40.0 38.0 41.0 34.0 41.0 24-25 38.405820238932165 40.0 38.0 41.0 34.0 41.0 26-27 38.362482042467946 40.0 38.0 41.0 34.0 41.0 28-29 38.244179819848625 40.0 38.0 41.0 34.0 41.0 30-31 38.086033624964436 40.0 38.0 41.0 34.0 41.0 32-33 37.90152277520579 40.0 38.0 41.0 33.0 41.0 34-35 37.64543573025729 40.0 38.0 41.0 33.0 41.0 36-37 37.44336764564117 40.0 37.0 41.0 32.5 41.0 38-39 37.282734435434 40.0 37.0 41.0 32.0 41.0 40-41 37.09237519074367 40.0 37.0 41.0 31.5 41.0 42-43 36.89579576261025 40.0 36.5 41.0 31.0 41.0 44-45 36.687958637132056 40.0 36.0 41.0 31.0 41.0 46-47 36.39022745815396 40.0 36.0 41.0 30.0 41.0 48-49 36.194631432676005 39.0 35.5 41.0 30.0 41.0 50-51 35.867696780458445 39.0 35.5 40.5 29.5 41.0 52-53 35.86942346623279 39.0 35.0 40.5 29.0 41.0 54-55 35.93544722778027 39.0 35.0 41.0 29.0 41.0 56-57 35.75540959631703 39.0 35.0 41.0 29.0 41.0 58-59 35.5082525293375 39.0 35.0 41.0 28.5 41.0 60-61 35.205402131156404 38.0 35.0 40.5 28.0 41.0 62-63 34.89001378997388 37.5 35.0 40.0 28.0 41.0 64-65 34.551459644634846 37.0 34.0 40.0 27.0 41.0 66-67 34.18789809291597 36.5 34.0 39.5 26.0 41.0 68-69 33.80409772659408 36.0 34.0 39.0 26.0 41.0 70-71 33.401383464728 35.5 34.0 39.0 26.0 40.5 72-73 33.014617901337616 35.0 33.0 37.5 25.5 40.0 74-75 32.61158592928906 35.0 33.0 37.0 24.5 39.5 76-77 32.236707311625196 35.0 33.0 36.5 24.0 39.0 78-79 31.863502477022585 35.0 32.5 36.0 22.5 39.0 80-81 31.49971021069387 35.0 32.0 36.0 20.5 37.0 82-83 31.175741049448753 35.0 32.0 35.0 20.0 37.0 84-85 30.880193870809222 35.0 32.0 35.0 18.5 36.5 86-87 30.583826112308934 34.5 31.0 35.0 17.5 36.0 88-89 30.280334121779696 34.0 31.0 35.0 12.5 36.0 90-91 29.953063243430073 34.0 31.0 35.0 7.0 35.5 92-93 29.66012740148927 34.0 31.0 35.0 2.0 35.0 94-95 29.29882508952315 34.0 30.0 35.0 2.0 35.0 96-97 28.892151353486526 34.0 30.0 35.0 2.0 35.0 98-99 28.423905913112584 34.0 29.5 35.0 2.0 35.0 100-101 25.154502667472357 30.5 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17706089536374492 1101 2 0.2132896362998835 1101 3 0.2998646637662077 1101 4 0.2141163598459528 1101 5 0.3299688226676665 1101 6 0.28599978663792314 1101 7 0.3601787231182456 1101 8 0.32794615256121773 1101 9 0.3793412721289897 1101 10-11 0.4833638627178871 1101 12-13 0.4063527009898493 1101 14-15 0.43791011041438566 1101 16-17 0.40717443904527073 1101 18-19 0.5519214003163526 1101 20-21 0.6092278352451643 1101 22-23 0.5992585530254786 1101 24-25 0.5280458105033219 1101 26-27 0.3504643563143759 1101 28-29 0.367734697092196 1101 30-31 0.35715816127871136 1101 32-33 0.3643000884851233 1101 34-35 0.174752442049801 1101 36-37 0.19517137541657092 1101 38-39 0.27625648845900486 1101 40-41 0.14584591520793566 1101 42-43 0.04635096366833835 1101 44-45 0.1261335529112415 1101 46-47 0.09350835074737063 1101 48-49 0.01588096475397549 1101 50-51 0.2348627574570017 1101 52-53 0.3349550503229679 1101 54-55 0.35598878457319927 1101 56-57 0.36095230195036265 1101 58-59 0.4083386729524179 1101 60-61 0.263469289475573 1101 62-63 0.373615925920447 1101 64-65 0.39544820969927486 1101 66-67 0.4050206808930099 1101 68-69 0.36309381221458636 1101 70-71 0.47313885319672266 1101 72-73 0.4506573740181352 1101 74-75 0.2723711513244922 1101 76-77 0.20784913570008712 1101 78-79 0.20926768973330923 1101 80-81 0.24508015291791807 1101 82-83 0.29901138291609186 1101 84-85 0.18466678989862828 1101 86-87 0.1786920067667772 1101 88-89 0.130244588668603 1101 90-91 0.15693203779018106 1101 92-93 0.27559321910695544 1101 94-95 0.5443947401024474 1101 96-97 0.4523603678610293 1101 98-99 0.5786424835727999 1101 100-101 0.556673324778167 1102 1 0.2361667905788991 1102 2 0.3239338222272181 1102 3 0.2499406814638121 1102 4 0.24492488066113793 1102 5 0.31353533388241317 1102 6 0.3310125131962849 1102 7 0.3080852469741373 1102 8 0.30693451929109017 1102 9 0.34717468233414905 1102 10-11 0.4118260844845665 1102 12-13 0.35976410193622854 1102 14-15 0.4171974231460638 1102 16-17 0.5294305418143566 1102 18-19 0.4449841448114782 1102 20-21 0.40848132437207596 1102 22-23 0.4839038322410474 1102 24-25 0.5343023232555026 1102 26-27 0.5094011335056479 1102 28-29 0.4466009230270416 1102 30-31 0.5704091563418316 1102 32-33 0.32433954935736153 1102 34-35 0.2127143136226053 1102 36-37 0.43091592178735993 1102 38-39 0.3608069590854157 1102 40-41 0.49090647312658575 1102 42-43 0.3209969261890464 1102 44-45 0.4486108757216769 1102 46-47 0.7265905694391819 1102 48-49 0.6052231127237064 1102 50-51 0.4218620229039587 1102 52-53 0.6608403575540436 1102 54-55 0.7511612337096736 1102 56-57 0.5737036301247471 1102 58-59 0.5728742816918881 1102 60-61 0.6416872296522058 1102 62-63 0.7693521018031859 1102 64-65 0.6911100761472824 1102 66-67 0.800346873687559 1102 68-69 0.7637412438413591 1102 70-71 0.6608214237906793 1102 72-73 0.7164972414569419 1102 74-75 0.7366727280511256 1102 76-77 0.7472374067672405 1102 78-79 0.7176723430413574 1102 80-81 0.658138114277012 1102 82-83 0.6269679588740011 1102 84-85 0.5498677670249883 1102 86-87 0.5973060931023824 1102 88-89 0.38356715994587276 1102 90-91 0.6116545304873604 1102 92-93 0.6076795205470233 1102 94-95 0.5828861731641659 1102 96-97 0.6099860533015047 1102 98-99 0.6326620261000002 1102 100-101 0.7153709451679902 1103 1 0.2575873773296635 1103 2 0.20147711481720876 1103 3 0.18262586908770118 1103 4 0.1531912643163622 1103 5 0.2190547909909384 1103 6 0.15598683362124888 1103 7 0.24673837215359384 1103 8 0.1996640584231315 1103 9 0.25991550795785656 1103 10-11 0.27909430877919306 1103 12-13 0.34172471320294306 1103 14-15 0.3423179843791715 1103 16-17 0.3887854847061831 1103 18-19 0.2691236361767011 1103 20-21 0.4347368818034312 1103 22-23 0.46571850493926803 1103 24-25 0.5065003783002524 1103 26-27 0.43319486272397967 1103 28-29 0.37355284058643434 1103 30-31 0.3716028302071308 1103 32-33 0.40208270870284935 1103 34-35 0.24731535514039393 1103 36-37 0.35770527759811444 1103 38-39 0.41643200577065187 1103 40-41 0.5027067721711234 1103 42-43 0.3744663565925421 1103 44-45 0.4596719829636413 1103 46-47 0.6512612253812335 1103 48-49 0.6074406881314118 1103 50-51 0.48791865484042063 1103 52-53 0.5393888128288395 1103 54-55 0.6596994171544566 1103 56-57 0.7241110275694354 1103 58-59 0.7670569890077772 1103 60-61 0.6264331950768565 1103 62-63 0.6268478391448511 1103 64-65 0.5224256000818599 1103 66-67 0.5839599154143329 1103 68-69 0.6176280240090861 1103 70-71 0.5942598357718083 1103 72-73 0.5378103604275211 1103 74-75 0.587953123104235 1103 76-77 0.4116022307364062 1103 78-79 0.4032690698724153 1103 80-81 0.6058946973801689 1103 82-83 0.6490653465304455 1103 84-85 0.6033374133139411 1103 86-87 0.5994453020894355 1103 88-89 0.551023247764622 1103 90-91 0.7783750965562959 1103 92-93 0.7149169509401538 1103 94-95 0.6809473922347955 1103 96-97 0.8306004805811362 1103 98-99 0.8342819873874205 1103 100-101 0.9538835720924475 1104 1 0.23291975603733306 1104 2 0.15577174706143637 1104 3 0.28364915085492015 1104 4 0.2033352677573319 1104 5 0.15907072540657907 1104 6 0.24053514192852532 1104 7 0.22173196807956685 1104 8 0.1278201044295031 1104 9 0.2353956889575386 1104 10-11 0.2324491924616936 1104 12-13 0.37917951621694357 1104 14-15 0.45038221859099536 1104 16-17 0.3547116591220245 1104 18-19 0.26185724371926966 1104 20-21 0.36035496317191473 1104 22-23 0.45909376540966207 1104 24-25 0.4275498742155506 1104 26-27 0.433729608095085 1104 28-29 0.38184097866366784 1104 30-31 0.4019199450067461 1104 32-33 0.5181326260207513 1104 34-35 0.43760623918493735 1104 36-37 0.4686078555441 1104 38-39 0.36710278322316015 1104 40-41 0.42282544578670667 1104 42-43 0.43521247584823186 1104 44-45 0.4442339090101797 1104 46-47 0.47589671213030726 1104 48-49 0.47721150739204177 1104 50-51 0.555361961659294 1104 52-53 0.47707561562031486 1104 54-55 0.5213400724168196 1104 56-57 0.5431175719726156 1104 58-59 0.5338644138310045 1104 60-61 0.4612745131245575 1104 62-63 0.35987642020548805 1104 64-65 0.49583218965606335 1104 66-67 0.4742875009268843 1104 68-69 0.39918308679808234 1104 70-71 0.40878103730479864 1104 72-73 0.5679076400765197 1104 74-75 0.5172855674095516 1104 76-77 0.5938237739168812 1104 78-79 0.6279640511957751 1104 80-81 0.6643963807071209 1104 82-83 0.7301035579898603 1104 84-85 0.6310461913169725 1104 86-87 0.5636287795369235 1104 88-89 0.3973721782531179 1104 90-91 0.623048173247831 1104 92-93 0.4526208174232025 1104 94-95 0.5202966801889524 1104 96-97 0.7609040191589891 1104 98-99 0.7270150967200166 1104 100-101 1.0204180608854223 1105 1 0.27933050896862 1105 2 0.19616574351434934 1105 3 0.24055330699155064 1105 4 0.24098527640826717 1105 5 0.245545239991813 1105 6 0.2616596339646051 1105 7 0.3447308566267395 1105 8 0.31844824343282596 1105 9 0.2851995537987193 1105 10-11 0.3325451834112343 1105 12-13 0.35759877340340296 1105 14-15 0.4539264686358777 1105 16-17 0.4407852321936616 1105 18-19 0.44668256977042375 1105 20-21 0.45117561082921753 1105 22-23 0.5076411172304773 1105 24-25 0.5789866387087059 1105 26-27 0.4445702238148286 1105 28-29 0.4248085779701327 1105 30-31 0.4548606352420492 1105 32-33 0.4397820900881655 1105 34-35 0.20538249477613135 1105 36-37 0.3162940441523361 1105 38-39 0.32060268455791885 1105 40-41 0.3450920234723185 1105 42-43 0.29636312734273673 1105 44-45 0.3435445084126769 1105 46-47 0.2640494678080145 1105 48-49 0.3134658992109749 1105 50-51 0.23760598613151274 1105 52-53 0.3104446062080157 1105 54-55 0.4074961839283944 1105 56-57 0.47703920948339373 1105 58-59 0.5183565471518534 1105 60-61 0.46490101797211736 1105 62-63 0.26212752750505075 1105 64-65 0.4096816293306773 1105 66-67 0.4795763226612806 1105 68-69 0.5455668536709481 1105 70-71 0.6030014420875673 1105 72-73 0.5733824468081181 1105 74-75 0.6561638874342179 1105 76-77 0.7544238449882741 1105 78-79 0.58407787239328 1105 80-81 0.5826746134525571 1105 82-83 0.7089790659537876 1105 84-85 0.6548388994894943 1105 86-87 0.6900151072489713 1105 88-89 0.6152134880737314 1105 90-91 0.5500146201766718 1105 92-93 0.5420698763755851 1105 94-95 0.5211512387055457 1105 96-97 0.528145821130753 1105 98-99 0.46802794913423185 1105 100-101 0.35003554363723666 1106 1 0.24027195844273308 1106 2 0.2523048705538429 1106 3 0.2355174236942119 1106 4 0.15409793718472287 1106 5 0.25723994904068803 1106 6 0.11381394126974698 1106 7 0.19021443395696735 1106 8 0.16855542112399746 1106 9 0.29842633639664484 1106 10-11 0.31210126698523055 1106 12-13 0.23125190972977094 1106 14-15 0.21775719216663703 1106 16-17 0.26883388396100116 1106 18-19 0.28691658299363354 1106 20-21 0.31638964479751763 1106 22-23 0.38151725934150704 1106 24-25 0.4463676381396837 1106 26-27 0.2421859269139759 1106 28-29 0.4063782609289248 1106 30-31 0.42900823332689697 1106 32-33 0.3305767128710997 1106 34-35 0.21874458871315738 1106 36-37 0.18513331094741403 1106 38-39 0.2148898080769328 1106 40-41 0.2040021759495474 1106 42-43 0.13319908000202219 1106 44-45 0.2760479586534217 1106 46-47 0.27080868584339157 1106 48-49 0.2007184203270853 1106 50-51 0.2213192229136567 1106 52-53 0.1451389890811683 1106 54-55 0.21112748790908853 1106 56-57 0.16325538859275213 1106 58-59 0.17481196940656218 1106 60-61 0.18897857954274144 1106 62-63 0.1271489114583204 1106 64-65 -0.05611991774219405 1106 66-67 -0.015253474720907434 1106 68-69 0.10786502748371873 1106 70-71 0.07956401028884841 1106 72-73 0.1562431824732542 1106 74-75 0.12456908656410093 1106 76-77 0.14776001946292183 1106 78-79 0.18821582012104443 1106 80-81 0.25174990131651853 1106 82-83 0.19935926304010465 1106 84-85 0.19360381250533365 1106 86-87 0.09599872545036803 1106 88-89 -0.02195233061541657 1106 90-91 0.031207534465469422 1106 92-93 -0.04293044503844712 1106 94-95 -0.00455950295137697 1106 96-97 -0.019465913893931486 1106 98-99 0.09347882159476484 1106 100-101 0.2933990184641715 1107 1 0.08923130349498365 1107 2 0.16453178460445628 1107 3 0.1769226934558148 1107 4 0.26760831872346813 1107 5 0.0749475485521458 1107 6 0.2502933470147113 1107 7 0.13183729710240044 1107 8 0.22770538965215792 1107 9 0.2134211049848318 1107 10-11 0.2634758152288583 1107 12-13 0.25063043468006896 1107 14-15 0.2905219620470447 1107 16-17 0.1338276104352829 1107 18-19 0.30186737437493605 1107 20-21 0.3161391206142241 1107 22-23 0.30805760790777015 1107 24-25 0.40012112401070254 1107 26-27 0.4069593505082736 1107 28-29 0.39844631513552287 1107 30-31 0.3721805114295833 1107 32-33 0.4335759166960784 1107 34-35 0.2232594430047854 1107 36-37 0.3752940882810165 1107 38-39 0.47191782426721574 1107 40-41 0.5031645247909253 1107 42-43 0.28674622731941923 1107 44-45 0.5268464263291364 1107 46-47 0.3995547727056703 1107 48-49 0.5099484232033191 1107 50-51 0.36283183040770695 1107 52-53 0.3908577767275361 1107 54-55 0.3385919830882713 1107 56-57 0.44744693746581277 1107 58-59 0.5641967887248498 1107 60-61 0.508747063517113 1107 62-63 0.6555008708408891 1107 64-65 0.4794011600536976 1107 66-67 0.45740208162202833 1107 68-69 0.47372683484271505 1107 70-71 0.6468708127915903 1107 72-73 0.4765216769938192 1107 74-75 0.5322446723363257 1107 76-77 0.48170125257546204 1107 78-79 0.46914367665947054 1107 80-81 0.6229783868749017 1107 82-83 0.839282229980082 1107 84-85 0.7266438255109158 1107 86-87 0.5856972210547333 1107 88-89 0.7111662577787676 1107 90-91 0.8886992913444622 1107 92-93 0.6071237448847278 1107 94-95 0.5939957103276541 1107 96-97 0.8881957075519402 1107 98-99 0.8668717777384458 1107 100-101 0.9204508365360908 1108 1 -0.06632824276223914 1108 2 -0.06940115165406269 1108 3 -0.032086315553193856 1108 4 -0.059170287124906906 1108 5 -0.07689066840234915 1108 6 -0.07545964850572062 1108 7 -0.13358427064474654 1108 8 -0.1380104923427936 1108 9 -0.037368555107150314 1108 10-11 -0.15961737763178263 1108 12-13 -0.21116258757808026 1108 14-15 -0.22274811866490296 1108 16-17 -0.09980602319835441 1108 18-19 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-1.1128544187233231 2316 72-73 -1.045806472331293 2316 74-75 -0.9499146356066035 2316 76-77 -1.197334810788199 2316 78-79 -1.2775910401460209 2316 80-81 -1.0907576299450383 2316 82-83 -1.2895459915680583 2316 84-85 -1.1818600344085155 2316 86-87 -1.295532014825664 2316 88-89 -1.1867408794610306 2316 90-91 -1.1759788347087898 2316 92-93 -1.3136291400238704 2316 94-95 -1.3331649618279648 2316 96-97 -1.5677378151662147 2316 98-99 -1.5592039440905623 2316 100-101 -1.4814117150231212 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9565.0 3 2078.0 4 1855.0 5 1575.0 6 1549.0 7 1648.0 8 1959.0 9 2335.0 10 2751.0 11 3465.0 12 4746.0 13 7657.0 14 8126.0 15 7254.0 16 8689.0 17 11620.0 18 10567.0 19 7326.0 20 6593.0 21 6876.0 22 7152.0 23 7800.0 24 8937.0 25 10341.0 26 12191.0 27 15050.0 28 18122.0 29 22850.0 30 28619.0 31 36272.0 32 47438.0 33 64501.0 34 92752.0 35 143348.0 36 243688.0 37 398441.0 38 365484.0 39 69962.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.817671162630965 17.60600486448596 12.776156021095119 23.800167951787955 2 15.949433614865164 18.83359170301184 39.99797772083259 25.2189969612904 3 20.180908811941602 23.40351236098521 27.99930397077618 28.416274856297008 4 12.921987341481952 14.72759261839218 35.318288190262216 37.03213184986365 5 14.268280280687481 37.34028801211445 33.2665337729681 15.124897934229963 6 34.704030322945485 34.05358950453633 16.485221208661883 14.757158963856302 7 30.828470959193677 31.51012428004924 19.274165009952043 18.38723975080504 8 28.063519235596452 34.612904306706696 18.426821375223312 18.896755082473543 9 27.502039962093157 15.587661933013994 19.07188191500119 37.83841618989167 10-11 26.53365271573007 25.720432635786505 25.854073595109334 21.89184105337409 12-13 26.793786261431197 22.552504950468858 26.99459732414556 23.659111463954385 14-15 23.53044915695627 24.089294091228137 24.865234101738995 27.5150226500766 16-17 23.473465322785465 27.393024108965374 25.54031761186729 23.593192956381866 18-19 23.507408177080226 25.86458615939066 27.558677400761113 23.069328262768 20-21 24.161250568355996 25.066799280616348 27.814130028095228 22.957820122932425 22-23 23.71121960395865 25.02649779405497 27.687769864527638 23.57451273745874 24-25 23.551433133751313 25.153243482228106 27.93505510062979 23.360268283390795 26-27 23.23456743676398 25.539539375954885 27.75254287304828 23.473350314232857 28-29 23.150824739661875 25.694680830424684 27.842055167637586 23.312439262275856 30-31 23.33125124007025 25.50404538473409 27.67710407771842 23.48759929747724 32-33 23.145377217815245 25.43661136378534 27.775316845445968 23.64269457295345 34-35 23.56119946081717 25.636964960482022 27.62398626998917 23.177849308711632 36-37 23.73493602468979 25.35028124340432 27.54369936548654 23.371083366419345 38-39 23.408266620303806 25.667275425213465 27.485345075180746 23.43911287930198 40-41 23.56483221500288 25.531828205310653 27.639241663008118 23.264097916678352 42-43 23.53433660300804 25.41853007225915 27.37280697740532 23.674326347327487 44-45 23.35498169101664 25.71554435935093 27.476466416405508 23.453007533226923 46-47 23.780497887574306 25.947786411734626 27.01536749829033 23.256348202400737 48-49 23.7555025134309 26.046042309740088 26.72147795583933 23.47697722098968 50-51 23.70390583977002 26.209090334542406 26.649011918874393 23.437991906813174 52-53 23.90528812123886 26.02007595589349 26.635176360773073 23.439459562094576 54-55 23.632358307950867 26.092282745156858 26.653570926630394 23.62178802026188 56-57 23.5231995880825 26.473700402056515 26.56498831148663 23.438111698374357 58-59 23.571852655328268 26.02215502018964 26.71678378144584 23.68920854303625 60-61 23.471844167193158 26.151888569387282 26.90268125483435 23.47358600858521 62-63 23.480610300668907 26.121227615745102 26.999504622246416 23.398657461339575 64-65 23.368976772957247 26.22814852433798 26.8456958442889 23.557178858415874 66-67 23.406347393981328 26.380526015317958 26.639347891670294 23.57377869903042 68-69 23.41209439989654 26.050117207073114 27.00457460431725 23.533213788713102 70-71 23.451244878812588 26.018052794504808 26.76482066766291 23.765881659019698 72-73 23.491116014674475 26.347064426091183 26.469824091717236 23.691995467517106 74-75 23.402108582708202 26.63785734217003 26.365876452584942 23.594157622536823 76-77 23.578916477049116 26.391485766062285 26.433705010302383 23.59589274658622 78-79 23.566778937224537 26.44659550949261 26.388610049553357 23.598015503729492 80-81 23.526518329909436 26.560520912742863 26.254407321216732 23.65855343613097 82-83 23.715576881742017 26.515130937168585 26.31675291883478 23.452539262254618 84-85 23.547904607083197 26.63476431369099 26.09304629876215 23.724284780463666 86-87 23.540448885521734 26.489253213643128 26.396190572919835 23.574107327915307 88-89 23.620808638167478 26.56414856934633 26.15744055959337 23.657602232892824 90-91 23.42591329076498 26.757273569875363 26.178826056366734 23.63798708299292 92-93 23.578724659715228 26.555694064578283 26.226048497058972 23.63953277864752 94-95 23.575733866741462 26.347842132845194 26.393664056214522 23.682759944198818 96-97 23.665775959040634 26.173285678064833 26.325767582276328 23.83517078061821 98-99 23.32857671994369 26.359626534524306 26.254085793171434 24.057710952360573 100-101 23.471130144800142 26.424720129897157 26.13238659457111 23.971763130731595 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 140.0 1 107.0 2 185.5 3 322.5 4 269.5 5 245.0 6 490.5 7 1015.5 8 1641.0 9 1878.0 10 1632.0 11 1442.5 12 1521.5 13 1655.5 14 1819.5 15 2023.5 16 2226.5 17 2337.0 18 2414.0 19 2532.0 20 2722.0 21 2940.0 22 3244.5 23 3752.0 24 4467.5 25 5549.5 26 7074.5 27 8712.0 28 10401.0 29 12166.0 30 13941.5 31 16827.5 32 20826.5 33 23294.0 34 24818.5 35 26983.5 36 30873.5 37 35053.5 38 38112.0 39 40568.5 40 42040.5 41 43636.0 42 44895.5 43 46845.5 44 50244.5 45 53958.5 46 56550.5 47 58918.0 48 62036.0 49 63534.0 50 62785.0 51 62939.0 52 64793.5 53 66164.0 54 67428.0 55 65666.0 56 61119.0 57 56600.5 58 54045.0 59 50720.0 60 43817.0 61 37203.5 62 31002.5 63 23890.5 64 17897.0 65 13842.0 66 10065.0 67 7765.0 68 6145.5 69 4559.0 70 3336.0 71 2181.5 72 1640.5 73 1358.5 74 1031.0 75 759.0 76 527.5 77 329.0 78 211.0 79 132.0 80 75.0 81 49.5 82 44.5 83 37.0 84 27.5 85 24.5 86 19.5 87 21.5 88 18.5 89 17.5 90 14.0 91 13.0 92 17.5 93 14.0 94 10.5 95 11.5 96 13.5 97 14.5 98 11.5 99 6.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043968032653905746 2 0.010874446578840326 3 0.009287365186252818 4 0.005231490516306968 5 0.007465160624393089 6 0.004820024970080577 7 0.004584901800808353 8 0.008523214886118092 9 0.013166897479244502 10-11 0.0021161085234500093 12-13 0.007906016566778506 14-15 0.008111749339891703 16-17 0.017163991356872296 18-19 0.010374809844136851 20-21 0.0033798955582882094 22-23 0.008875899640026427 24-25 0.02045571572668342 26-27 0.006407106362668083 28-29 0.01616471788746535 30-31 0.013137507083085476 32-33 0.012314575990632692 34-35 0.2482606763553087 36-37 0.436065307811497 38-39 0.4321269947261873 40-41 0.45346442233764156 42-43 0.4432365644742998 44-45 0.45878408404242565 46-47 0.46566143674363814 48-49 0.47215671429478334 50-51 0.48241396255428404 52-53 0.47121622161769444 54-55 0.45907798800401595 56-57 0.45220063530280336 58-59 0.45081928668332905 60-61 0.4482623222174936 62-63 0.445499624978545 64-65 0.46104714454667073 66-67 0.4353893286998394 68-69 0.462340321977668 70-71 0.4430308317011866 72-73 0.4516128273796228 74-75 0.46545570397052494 76-77 0.4523475872835985 78-79 0.45308234718757423 80-81 0.4553747980879784 82-83 0.4556687020495687 84-85 0.45466942858016174 86-87 0.45572748284188674 88-89 0.4706284136945139 90-91 0.4954632984488925 92-93 0.48232579136580694 94-95 0.4539934494685041 96-97 0.4270130657945165 98-99 0.4175493582313095 100-101 0.4279535584716053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1701236.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.89098604406557 #Duplication Level Percentage of deduplicated Percentage of total 1 70.87263061921306 33.94160163890946 2 14.387873244802703 13.780988735412613 3 5.638165353832935 8.100518948236415 4 2.840200358121285 5.440799828525459 5 1.6124769822353737 3.861155632630563 6 1.056593482215349 3.0360782226615552 7 0.7123490192049414 2.3880567856073354 8 0.49987264660736547 1.915151515398677 9 0.36609021610960063 1.577917928751645 >10 1.8342444184795956 15.709639275610918 >50 0.12351009186977821 4.075706921410684 >100 0.05328041522531323 4.565023017226231 >500 0.001973201514842054 0.704250564675776 >1k 6.166254733881418E-4 0.5940416118340284 >5k 1.2332509467762837E-4 0.3090693731087165 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5233 0.30759988620038603 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3902 0.2293626516250538 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2551 0.14994980120336038 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.7634237695416744E-4 2 0.0 0.0 0.0 0.0 1.7634237695416744E-4 3 0.0 0.0 0.0 0.0 1.7634237695416744E-4 4 0.0 0.0 0.0 0.0 1.7634237695416744E-4 5 0.0 0.0 0.0 0.0 1.7634237695416744E-4 6 0.0 0.0 0.0 0.0 2.9390396159027906E-4 7 0.0 0.0 0.0 0.0 2.9390396159027906E-4 8 0.0 0.0 0.0 0.0 2.9390396159027906E-4 9 0.0 0.0 0.0 5.878079231805581E-5 2.9390396159027906E-4 10-11 0.0 0.0 0.0 1.7634237695416744E-4 3.526847539083349E-4 12-13 0.0 0.0 0.0 1.7634237695416744E-4 0.0010874446578840325 14-15 0.0 0.0 0.0 2.3512316927222325E-4 0.0013225678271562558 16-17 0.0 0.0 0.0 3.526847539083349E-4 0.0014107390156333395 18-19 0.0 0.0 0.0 3.820751500673628E-4 0.0015283006002694511 20-21 0.0 0.0 0.0 6.17198319339586E-4 0.0016752525810645907 22-23 0.0 0.0 0.0 8.229310924527814E-4 0.0017046429772236186 24-25 0.0 0.0 0.0 0.0013519582233152837 0.0017046429772236186 26-27 0.0 0.0 0.0 0.0016164717887465348 0.0018222045618597302 28-29 0.0 0.0 0.0 0.0019985469388138976 0.001851594958018758 30-31 0.0 0.0 0.0 0.0034974571429243206 0.001910375750336814 32-33 0.0 0.0 0.0 0.006054421608759749 0.0019397661464958418 34-35 0.0 0.0 0.0 0.008787728451549345 0.0021161085234500093 36-37 0.0 0.0 0.0 0.014577636494877842 0.0021161085234500093 38-39 0.0 0.0 0.0 0.025275740696764 0.002233670108086121 40-41 0.0 0.0 0.0 0.03773726866819183 0.0022924509004041767 42-43 0.0 0.0 0.0 0.04908196158557661 0.0023512316927222325 44-45 0.0 0.0 0.0 0.06342447491118222 0.0024100124850402883 46-47 0.0 0.0 0.0 0.081088103002758 0.002468793277358344 48-49 0.0 0.0 0.0 0.10119113397553309 0.0025275740696764 50-51 0.0 0.0 0.0 0.12517369724129984 0.002762697238948623 52-53 0.0 0.0 0.0 0.14821576782997775 0.003115381992856958 54-55 0.0 0.0 0.0 0.17263918703812992 0.003115381992856958 56-57 0.0 0.0 0.0 0.20373422617438144 0.003174162785175014 58-59 0.0 0.0 0.0 0.2385324552266705 0.0032623339736520974 60-61 0.0 0.0 0.0 0.27621094310254424 0.0033505051621291813 62-63 0.0 0.0 0.0 0.31956177743711045 0.003820751500673628 64-65 0.0 0.0 0.0 0.3627068789985634 0.003820751500673628 66-67 0.0 0.0 0.0 0.41258238128043373 0.0038501418968326555 68-69 0.0 0.0 0.0 0.47409648044127917 0.0038795322929916836 70-71 0.0 0.0 0.0 0.5415768300224072 0.003908922689150712 72-73 0.0 0.0 0.0 0.6112614593154624 0.0042322170469000185 74-75 0.0 0.0 0.0 0.6830328067358086 0.004526121008490298 76-77 0.0 0.0 0.0 0.7610643085380276 0.00464368259312641 78-79 0.0 0.0 0.0 0.8579644446743426 0.004702463385444465 80-81 0.0 0.0 0.0 0.9674142799705625 0.004731853781603493 82-83 0.0 0.0 0.0 1.0863278228299895 0.004937586554716688 84-85 0.0 0.0 0.0 1.2029489147890122 0.004937586554716688 86-87 0.0 0.0 0.0 1.3266824826185197 0.005084538535511828 88-89 0.0 0.0 0.0 1.468020897747285 0.005231490516306968 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2820 0.0 50.542892 1 GTATCAA 5370 0.0 42.76713 1 ATATGCG 80 1.10291185E-5 35.506 5 ATCAACG 6505 0.0 34.714558 3 CAACGCA 6675 0.0 34.114136 5 TCAACGC 6655 0.0 34.074383 4 TATCAAC 6740 0.0 33.573433 2 AACGCAG 6945 0.0 32.98947 6 ACGCAGA 8385 0.0 27.212708 7 TACATGG 8055 0.0 26.917068 2 GTACATG 8335 0.0 26.47421 1 CGCAGAG 8670 0.0 26.318947 8 TACCTGG 1405 0.0 25.944311 2 ACATGGG 8200 0.0 25.518135 3 GTACCGT 170 1.1614429E-7 25.060852 6 GTACCTG 1595 0.0 24.04734 1 AGTACAT 5800 0.0 23.669973 2 GAGTACA 6165 0.0 23.272991 1 GCAGAGT 9620 0.0 22.834076 9 CATGGGG 4870 0.0 22.455542 4 >>END_MODULE