FastQCFastQC Report
Thu 26 May 2016
SRR936285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701236
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55740.3276441363808431No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41800.24570371188947332No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29110.1711108864378605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30300.051.181251
GTATCAA56250.042.0310061
ATCAACG72100.032.3725623
TCAACGC72850.032.039284
TATCAAC73450.031.8496362
CAACGCA73200.031.821285
AACGCAG76700.030.3073486
TACATGG80000.026.098292
GTACATG83600.025.7198311
ACGCAGA92350.025.0172337
CGCAGAG93450.024.6212238
ACATGGG82300.024.5559653
GTATAGG3950.024.086231
TGGTATC15250.023.9590842
GTGGTAT16700.022.7881681
CATGGGG48700.022.5024724
TACCTGG12550.022.3078442
GCAGAGT108450.020.997089
GTACCTG14150.020.1711541
GAGTACA62650.020.0455881
Produced by FastQC (version 0.11.5)