##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936285_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1701236 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30921870922082 34.0 31.0 34.0 30.0 34.0 2 32.451527595230765 34.0 31.0 34.0 31.0 34.0 3 32.527829766122984 34.0 31.0 34.0 31.0 34.0 4 36.06893752542269 37.0 35.0 37.0 35.0 37.0 5 36.02279460345302 37.0 35.0 37.0 35.0 37.0 6 36.06757145980922 37.0 35.0 37.0 35.0 37.0 7 36.00855848336151 37.0 35.0 37.0 35.0 37.0 8 35.98522544785086 37.0 35.0 37.0 35.0 37.0 9 37.7174025238121 39.0 37.0 39.0 35.0 39.0 10-11 37.642964879652205 39.0 37.0 39.0 35.0 39.0 12-13 37.578383304844245 39.0 37.0 39.0 35.0 39.0 14-15 38.85006900865018 40.0 38.0 41.0 35.0 41.0 16-17 38.76297732942402 40.0 38.0 41.0 34.5 41.0 18-19 38.7937384936599 40.0 38.0 41.0 35.0 41.0 20-21 38.743042117613314 40.0 38.0 41.0 34.0 41.0 22-23 38.613350528674445 40.0 38.0 41.0 34.0 41.0 24-25 38.77391761049026 40.0 38.0 41.0 35.0 41.0 26-27 38.67658749285813 40.0 38.0 41.0 34.0 41.0 28-29 38.57292345094978 40.0 38.0 41.0 34.0 41.0 30-31 38.42861484238519 40.0 38.0 41.0 34.0 41.0 32-33 38.251103609375775 40.0 38.0 41.0 34.0 41.0 34-35 38.090222344225026 40.0 37.5 41.0 33.0 41.0 36-37 37.91840550047142 40.0 37.0 41.0 33.0 41.0 38-39 37.762803926086676 40.0 37.0 41.0 33.0 41.0 40-41 37.58628432504368 40.0 37.0 41.0 33.0 41.0 42-43 37.59295712058762 40.0 37.0 41.0 33.0 41.0 44-45 37.42520320519904 40.0 37.0 41.0 32.0 41.0 46-47 37.176529005969776 40.0 36.5 41.0 31.5 41.0 48-49 36.8908308429871 40.0 36.0 41.0 31.0 41.0 50-51 36.64607849822129 39.5 36.0 41.0 30.5 41.0 52-53 36.39630245304002 39.0 35.0 41.0 30.0 41.0 54-55 36.16729307397681 39.0 35.0 41.0 30.0 41.0 56-57 35.880300851851246 38.5 35.0 40.0 29.0 41.0 58-59 35.57965267605435 38.0 35.0 40.0 28.5 41.0 60-61 35.26764922679746 38.0 34.5 40.0 28.0 41.0 62-63 34.93924035230856 37.0 34.0 40.0 28.0 41.0 64-65 34.53341129625755 36.5 34.0 39.5 27.0 41.0 66-67 34.163248661561354 36.0 34.0 39.0 26.0 41.0 68-69 33.79705284863476 35.5 33.0 39.0 26.0 40.5 70-71 33.40538320374128 35.0 33.0 38.0 26.0 40.0 72-73 32.989453550242295 35.0 33.0 37.0 26.0 39.5 74-75 32.55413622801304 35.0 32.0 37.0 25.0 39.0 76-77 31.87553725644179 34.5 31.5 36.0 24.0 39.0 78-79 31.97351837134883 35.0 32.0 36.0 24.0 38.5 80-81 31.81800731938426 35.0 32.0 35.5 24.0 37.0 82-83 31.52285573547703 35.0 32.0 35.0 24.0 37.0 84-85 31.214187508376263 35.0 32.0 35.0 23.0 36.5 86-87 30.78375222485299 34.0 31.0 35.0 20.0 36.0 88-89 30.26554781347209 34.0 31.0 35.0 18.0 36.0 90-91 30.00756979043472 34.0 31.0 35.0 16.0 35.0 92-93 29.66938625799125 34.0 30.0 35.0 9.0 35.0 94-95 29.450612672198332 34.0 30.0 35.0 4.5 35.0 96-97 29.11346632683531 34.0 30.0 35.0 2.0 35.0 98-99 28.535811609911853 34.0 29.5 35.0 2.0 35.0 100-101 24.95974955855625 30.0 23.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15523108565679422 1101 2 0.12845445006099254 1101 3 0.1701402368711129 1101 4 0.1330130877175293 1101 5 0.11942636428178588 1101 6 0.16365332087436713 1101 7 0.10633599906415014 1101 8 0.09562167182318859 1101 9 0.22542241684960374 1101 10-11 0.10607341764639955 1101 12-13 0.22465036036054897 1101 14-15 0.32876112751860376 1101 16-17 0.28775108362150803 1101 18-19 0.2961649412149896 1101 20-21 0.1251384903559014 1101 22-23 0.1974726951341026 1101 24-25 0.11779949682750868 1101 26-27 0.22002099626079996 1101 28-29 0.3037184945468354 1101 30-31 0.3037029118261998 1101 32-33 0.29216940439306427 1101 34-35 0.23489150353019284 1101 36-37 0.2451722431322665 1101 38-39 0.23621165550609646 1101 40-41 0.2785186542823155 1101 42-43 0.1743926292330542 1101 44-45 0.24514565268206923 1101 46-47 0.2951934755186514 1101 48-49 0.22597561459716786 1101 50-51 0.08484088149980806 1101 52-53 0.2631409529231732 1101 54-55 0.1407545763048006 1101 56-57 0.07499043215192813 1101 58-59 -0.05847328446454725 1101 60-61 0.15420295237664305 1101 62-63 0.07176915268873785 1101 64-65 0.14257289932395167 1101 66-67 0.18946443070303332 1101 68-69 0.2253856170587838 1101 70-71 0.18557978170576916 1101 72-73 0.15717846457851437 1101 74-75 -0.021135384054531414 1101 76-77 -0.09386552755289657 1101 78-79 0.04435786344495085 1101 80-81 0.25171760783214836 1101 82-83 0.23183477025025923 1101 84-85 0.34371300950832406 1101 86-87 0.29621407790019205 1101 88-89 0.27452203384671847 1101 90-91 0.6727919717260171 1101 92-93 0.4505965361541264 1101 94-95 0.33796103360831964 1101 96-97 0.26366972402021815 1101 98-99 0.3813728819401163 1101 100-101 0.596919648213941 1102 1 0.1104404332712079 1102 2 0.1901312808917197 1102 3 0.18233723374498823 1102 4 0.18027217994664113 1102 5 0.10741727632333209 1102 6 0.17451787271160413 1102 7 0.08463251933268623 1102 8 0.22692729713281068 1102 9 0.07338702164299349 1102 10-11 0.13362769769901206 1102 12-13 0.20187067502256895 1102 14-15 0.10180986425821459 1102 16-17 0.258518435301589 1102 18-19 0.3588069405658487 1102 20-21 0.3282680279291341 1102 22-23 0.3340056048182376 1102 24-25 0.3041624343563285 1102 26-27 0.35501219870703693 1102 28-29 0.21329587031342356 1102 30-31 0.2158853884657077 1102 32-33 0.2870787810479456 1102 34-35 0.32520309067722764 1102 36-37 0.21260421156357268 1102 38-39 0.1223943713024056 1102 40-41 0.03594105644156542 1102 42-43 0.10452894860988238 1102 44-45 0.13810590140514023 1102 46-47 0.2553781387346348 1102 48-49 0.1519992911205108 1102 50-51 -0.04231366504181011 1102 52-53 0.050022348232282354 1102 54-55 -0.01798147999954125 1102 56-57 0.23830036228397944 1102 58-59 0.10839410308904718 1102 60-61 0.012122459030329935 1102 62-63 0.07438706553316621 1102 64-65 0.12230863105835255 1102 66-67 0.1591513061194192 1102 68-69 0.10437654618295511 1102 70-71 -0.05664762224714792 1102 72-73 -0.13115495416796108 1102 74-75 0.16035473364478037 1102 76-77 0.05581246489657943 1102 78-79 0.0824136894754659 1102 80-81 0.13422328780618642 1102 82-83 0.19835708870461843 1102 84-85 0.20934532300872277 1102 86-87 0.04153343764327033 1102 88-89 0.19801321066900712 1102 90-91 0.4092796124441769 1102 92-93 0.13052786398685257 1102 94-95 0.3849083678641172 1102 96-97 0.3111338077934285 1102 98-99 0.45384593409338336 1102 100-101 0.8081292691504096 1103 1 -0.00849906828381819 1103 2 0.08848420439098703 1103 3 0.16044515315753927 1103 4 0.025399407722638045 1103 5 0.11118663639134496 1103 6 0.11500472895736635 1103 7 0.1398594732363918 1103 8 0.18173124507332972 1103 9 0.21022601815558062 1103 10-11 0.2502695303420239 1103 12-13 0.23380099755975436 1103 14-15 0.19114866323560875 1103 16-17 0.29194419639497227 1103 18-19 0.2501869797102074 1103 20-21 0.17218435418042333 1103 22-23 0.21783936783559454 1103 24-25 0.28238239832072765 1103 26-27 0.2874879774382535 1103 28-29 0.24033595549318676 1103 30-31 0.22751297914514623 1103 32-33 0.23269529162222824 1103 34-35 0.2110493379187517 1103 36-37 0.2711141356021045 1103 38-39 0.30705026931465795 1103 40-41 0.29865552245104254 1103 42-43 0.28597333457335594 1103 44-45 0.2643438425832585 1103 46-47 0.30404390184870067 1103 48-49 0.330476547371795 1103 50-51 0.25969173813938795 1103 52-53 0.16074805582924512 1103 54-55 0.17464167077961434 1103 56-57 0.18381195252647586 1103 58-59 0.30360485694810535 1103 60-61 0.11425916374609102 1103 62-63 0.06176467467081892 1103 64-65 0.12891472602291287 1103 66-67 0.13205746546102404 1103 68-69 0.05581807814069606 1103 70-71 -0.0599120262327375 1103 72-73 -0.1206329129105228 1103 74-75 0.04005493782924674 1103 76-77 0.11330664786295941 1103 78-79 0.10111433536157222 1103 80-81 0.24162457526068692 1103 82-83 0.2613483630462454 1103 84-85 0.12529571771210257 1103 86-87 0.10670580275047925 1103 88-89 0.12059344773714642 1103 90-91 0.20930815250150303 1103 92-93 0.34586864133856565 1103 94-95 0.32430154838796454 1103 96-97 0.29276066014461577 1103 98-99 0.20139898038829074 1103 100-101 0.5563139962906227 1104 1 0.08323840993506337 1104 2 0.0922622966868758 1104 3 0.10163852380956939 1104 4 0.05812640567955896 1104 5 0.0735709530735349 1104 6 0.14602992468122977 1104 7 0.16865850073052258 1104 8 0.0815648844598158 1104 9 0.11799448171338867 1104 10-11 0.14330862363308228 1104 12-13 0.23314045417044582 1104 14-15 0.2651664734117958 1104 16-17 0.25968613857521206 1104 18-19 0.2946991345804335 1104 20-21 0.2557530571281248 1104 22-23 0.3551966877648667 1104 24-25 0.36579271201448904 1104 26-27 0.4549116464806957 1104 28-29 0.3734705307255908 1104 30-31 0.3170946612929981 1104 32-33 0.2339866933899586 1104 34-35 0.23273558769714953 1104 36-37 0.2780666557764988 1104 38-39 0.3659415943580626 1104 40-41 0.5100130076199534 1104 42-43 0.45056966201853754 1104 44-45 0.4031696364081938 1104 46-47 0.26822820964614635 1104 48-49 0.2511943184800174 1104 50-51 0.2951022195638373 1104 52-53 0.15604391574589016 1104 54-55 0.17847456617253243 1104 56-57 0.2601493453023451 1104 58-59 0.3721537747322472 1104 60-61 0.3377808605828321 1104 62-63 0.31907787810697386 1104 64-65 0.2189437592090897 1104 66-67 0.1275270761057783 1104 68-69 0.2982763208793884 1104 70-71 0.19727662471014185 1104 72-73 0.052276463110551674 1104 74-75 0.1322385215615327 1104 76-77 0.00521625762754141 1104 78-79 0.04869455028104852 1104 80-81 0.11667060778633243 1104 82-83 0.06565418425612535 1104 84-85 0.2526888381796901 1104 86-87 0.2817740886549167 1104 88-89 0.25515726282983664 1104 90-91 0.5309452455625596 1104 92-93 0.46737312751178806 1104 94-95 0.15523295608439724 1104 96-97 0.22433068999154315 1104 98-99 0.07746115367153195 1104 100-101 0.40797280715475637 1105 1 -0.06304669169418986 1105 2 -0.0028044006126179966 1105 3 0.09149119856962074 1105 4 0.0860958749658991 1105 5 -0.032860278467254034 1105 6 0.045089505116898465 1105 7 0.0364896198699185 1105 8 0.039990146652066016 1105 9 0.138858027478868 1105 10-11 0.1470048222050906 1105 12-13 0.09924543804161345 1105 14-15 0.14018192151205255 1105 16-17 0.08390134469237154 1105 18-19 0.1494902337193551 1105 20-21 0.09597127952485351 1105 22-23 0.03510556727211167 1105 24-25 0.2208437444338216 1105 26-27 0.20031520353493448 1105 28-29 0.08376405070844584 1105 30-31 0.2884029300076918 1105 32-33 0.25956307467298245 1105 34-35 0.2790578052866266 1105 36-37 0.20612044636549598 1105 38-39 0.1977072240874591 1105 40-41 0.2186535302256445 1105 42-43 0.3976299847734168 1105 44-45 0.2880232451451974 1105 46-47 0.3278503805165158 1105 48-49 0.48983900244936507 1105 50-51 0.3609806177913484 1105 52-53 0.435642982336212 1105 54-55 0.466347331364247 1105 56-57 0.40716501545005457 1105 58-59 0.29897293288878757 1105 60-61 0.4248171795223854 1105 62-63 0.5261790608337904 1105 64-65 0.42884241561943526 1105 66-67 0.36588508385393936 1105 68-69 0.5135501057875373 1105 70-71 0.361126570879847 1105 72-73 0.3578490031348096 1105 74-75 0.39964260456027034 1105 76-77 0.3460459208974491 1105 78-79 0.09737419861948382 1105 80-81 0.31035676154770897 1105 82-83 0.3526693519606994 1105 84-85 0.2106392105751418 1105 86-87 0.38637059238008575 1105 88-89 0.6640278557654042 1105 90-91 0.822241917565659 1105 92-93 0.7216646604131007 1105 94-95 0.1658290359744754 1105 96-97 0.13740438178563608 1105 98-99 0.17508848755025852 1105 100-101 0.3780055186336746 1106 1 0.006916809956926784 1106 2 0.0705272026402497 1106 3 -0.014121087223799123 1106 4 -0.007486796294209341 1106 5 -0.01660585505624823 1106 6 0.055962029137639036 1106 7 0.06041988651659125 1106 8 0.1305900163404985 1106 9 0.023844976481967706 1106 10-11 0.1171744256436682 1106 12-13 0.13964281689034408 1106 14-15 0.17538231324036246 1106 16-17 0.09793205917592473 1106 18-19 0.24341356417186688 1106 20-21 0.2312770633108201 1106 22-23 0.15688696287564596 1106 24-25 0.1505818435833035 1106 26-27 0.08772029711403917 1106 28-29 0.12082447733994428 1106 30-31 0.06698695438119273 1106 32-33 0.1769414154940776 1106 34-35 0.25595607258723874 1106 36-37 0.2675067566673306 1106 38-39 0.34540232835654905 1106 40-41 0.041157988357298336 1106 42-43 0.10784626863994617 1106 44-45 0.15131792149713874 1106 46-47 0.282929198379513 1106 48-49 0.2106613541366329 1106 50-51 0.3002801891599418 1106 52-53 0.24404649106637777 1106 54-55 0.16248290300902823 1106 56-57 0.3031935284458953 1106 58-59 0.3937954290139629 1106 60-61 0.2582465402576517 1106 62-63 0.2924025332640028 1106 64-65 0.2701841168281902 1106 66-67 0.3558944643819615 1106 68-69 0.3523064080818017 1106 70-71 0.24691725336484183 1106 72-73 0.1635573956916474 1106 74-75 0.23555637671502438 1106 76-77 0.38553639632832315 1106 78-79 0.23970029974030638 1106 80-81 0.24707172761669938 1106 82-83 0.14532615240702995 1106 84-85 0.45357167315421165 1106 86-87 0.33896083234542473 1106 88-89 0.3144893031443736 1106 90-91 0.4882482966421584 1106 92-93 0.46557426132797985 1106 94-95 0.5650927200776543 1106 96-97 0.35832672894929374 1106 98-99 0.13244894822782882 1106 100-101 0.29015651656472485 1107 1 0.00960046169079476 1107 2 0.0548146259927762 1107 3 0.034874523392424805 1107 4 0.04270099263187177 1107 5 0.05652761472452994 1107 6 0.11801866177581388 1107 7 0.16339949860010705 1107 8 -0.027509501369188172 1107 9 0.08652687746447185 1107 10-11 0.024197439687192457 1107 12-13 0.1153507248675183 1107 14-15 0.23106787829536302 1107 16-17 0.23374868047590525 1107 18-19 0.24865977400358474 1107 20-21 0.1808174910777396 1107 22-23 0.11511653640095432 1107 24-25 0.13573297094212933 1107 26-27 0.22590668543902126 1107 28-29 0.21245567739662619 1107 30-31 0.18381396764104352 1107 32-33 0.1237398331483135 1107 34-35 0.2263175193748168 1107 36-37 0.22909089485997924 1107 38-39 0.24749635204171483 1107 40-41 0.3491426651144067 1107 42-43 0.2846250528681722 1107 44-45 0.18897033065606195 1107 46-47 0.19930101628469998 1107 48-49 0.1431329982172187 1107 50-51 0.23136102801995406 1107 52-53 0.21682189050863343 1107 54-55 0.2133880831488355 1107 56-57 0.021272954199439198 1107 58-59 0.15963347617586265 1107 60-61 0.266756025578438 1107 62-63 0.3164230504026051 1107 64-65 0.31218005675241045 1107 66-67 0.07811629291671096 1107 68-69 0.24872549627381346 1107 70-71 0.5233690222489855 1107 72-73 0.42854987162923663 1107 74-75 0.31380370786131095 1107 76-77 0.33160512582473345 1107 78-79 0.3166712497730302 1107 80-81 0.5401856473405786 1107 82-83 0.7478078424656829 1107 84-85 0.5213568059403677 1107 86-87 0.5820213958372555 1107 88-89 0.5756301260808847 1107 90-91 0.35246543983278045 1107 92-93 0.5916271090084457 1107 94-95 0.7043979218934346 1107 96-97 0.499815993091822 1107 98-99 0.49382216256565314 1107 100-101 0.6213676196631361 1108 1 -0.1456138888723899 1108 2 -0.09077814285581098 1108 3 -0.03339041929112341 1108 4 -0.0856918520400427 1108 5 -0.12870678911452416 1108 6 -0.13556249413092303 1108 7 -0.03950174151043484 1108 8 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738.0 10 1078.0 11 1860.0 12 2740.0 13 3940.0 14 5022.0 15 6900.0 16 8839.0 17 12116.0 18 11188.0 19 7607.0 20 7077.0 21 7217.0 22 7894.0 23 8887.0 24 10362.0 25 12707.0 26 15477.0 27 19166.0 28 23650.0 29 29494.0 30 36244.0 31 44407.0 32 56078.0 33 72078.0 34 99432.0 35 147820.0 36 246580.0 37 401024.0 38 342840.0 39 50102.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.653392069653925 18.32104160332242 13.46663319733471 23.558933129688945 2 16.233250083485018 19.42913885792496 39.37141661140194 24.966194447188084 3 20.226705759812276 23.629643388689164 28.54465811915572 27.598992732342836 4 13.28163758584935 15.263549560437234 35.29992311472365 36.15488973898977 5 14.15229868166439 37.475576580791845 33.2793921595828 15.092732577960966 6 34.60736781963231 34.55428876416911 16.163483490826668 14.674859925371905 7 30.558370502387678 31.76390577203868 19.264111504811794 18.413612220761845 8 27.94932625455845 34.95082398914671 18.257314093988136 18.8425356623067 9 27.32713156787183 15.59989325408115 19.028988335539573 38.04398684250745 10-11 26.46149532528993 25.83189148312528 25.819753195999244 21.886859995585542 12-13 26.732953785645787 22.67887073835457 27.061185826418505 23.526989649581143 14-15 23.33917810344949 24.27132390802922 24.952064263864628 27.437433724656664 16-17 23.2950696279524 27.570441339026893 25.637476153150047 23.497012879870656 18-19 23.36072126383406 25.930147257640918 27.78027269585172 22.928858782673302 20-21 23.92279023098213 25.140199578482815 28.069188539740676 22.86782165079438 22-23 23.619540034286846 25.009911914017785 27.99403727467479 23.376510777020584 24-25 23.428946290234123 25.199991300253526 28.194820241488504 23.176242168023848 26-27 23.145956059672223 25.600732662098697 27.981326051386862 23.271985226842215 28-29 23.13446851409081 25.704804997656062 28.084364102345553 23.076362385907572 30-31 23.239336029047404 25.423093675770136 27.960685837625842 23.37688445755661 32-33 23.06632882939149 25.431464069618542 28.044804488090413 23.457402612899557 34-35 23.47066985537651 25.605267543316952 27.93910571325536 22.984956888051183 36-37 23.641316726951356 25.3144556582033 27.83045360910187 23.213774005743478 38-39 23.32930782282385 25.596545095073665 27.797947783182025 23.27619929892046 40-41 23.482561358835003 25.497436548551573 27.892126953448752 23.127875139164672 42-43 23.40347676114417 25.410464847655835 27.575548986263072 23.610509404936924 44-45 23.30567095832715 25.70505255949223 27.656095005341918 23.3331814768387 46-47 23.637816249703487 25.93430548153905 27.21860165977909 23.209276608978374 48-49 23.719366048348835 26.05038879773406 26.924955893929088 23.30528925998802 50-51 23.658130400074302 26.26568512533404 26.77693232867017 23.299252145921486 52-53 23.900501846727767 25.989687952092655 26.791719793523903 23.318090407655674 54-55 23.653695471102985 26.094192969679014 26.75377417881522 23.498337380402777 56-57 23.53546462824802 26.477408728488562 26.627274586935716 23.359852056327696 58-59 23.560761386587913 26.04898742778678 26.818086164452165 23.572165021173138 60-61 23.573193699550675 26.09898119693489 26.982939222154556 23.34488588135988 62-63 23.484454831663566 26.137907968089085 27.127100531613486 23.250536668633863 64-65 23.36741075837162 26.196050608267928 26.980691549143444 23.45584708421701 66-67 23.41156664918918 26.390106957529703 26.766980007476914 23.431346385804204 68-69 23.446893905372328 26.034248040836193 27.124249663186063 23.394608390605416 70-71 23.517657324725075 26.030038774783844 26.807033363125427 23.645270537365658 72-73 23.503838421838445 26.32972219936281 26.574664064612453 23.59177531418629 74-75 23.39487290417085 26.586581755852805 26.46522881011218 23.55331652986417 76-77 23.574639530515213 26.426626514395814 26.476355580784855 23.522378374304118 78-79 23.572575036743203 26.44634080043115 26.514061262601242 23.467022900224404 80-81 23.549312713615144 26.523061838874995 26.337400905644454 23.59022454186541 82-83 23.710378806938014 26.522863376980034 26.38020239402411 23.386555422057846 84-85 23.562927356864382 26.66095510447603 26.145768276141784 23.6303492625178 86-87 23.525505298082617 26.48240975369771 26.40243536433366 23.589649583886015 88-89 23.581445414790373 26.582706851362104 26.205724108334582 23.63012362551294 90-91 23.393049224709426 26.794741448456982 26.21776525414161 23.594444072691985 92-93 23.581918159653284 26.539605606304264 26.27194678538249 23.606529448659966 94-95 23.4843967080797 26.330395301421856 26.48943052106592 23.695777469432524 96-97 23.588114565585354 26.15954758767605 26.417895927648356 23.83444191909024 98-99 23.26436404591604 26.406455003315322 26.30026475553612 24.02891619523252 100-101 23.268256467862162 26.48929973154544 26.263549842789203 23.978893957803198 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 165.0 1 121.0 2 163.5 3 303.5 4 250.5 5 210.0 6 473.5 7 968.5 8 1522.5 9 1734.0 10 1490.5 11 1271.0 12 1339.5 13 1470.0 14 1565.5 15 1628.5 16 1715.5 17 1821.0 18 1979.0 19 2206.5 20 2376.0 21 2631.5 22 3073.0 23 3765.0 24 4670.5 25 5764.5 26 7205.5 27 8891.0 28 10691.5 29 12526.0 30 14313.5 31 17163.5 32 21343.5 33 23886.0 34 25362.0 35 27566.5 36 31338.5 37 35460.5 38 38515.5 39 40749.5 40 42537.5 41 44396.0 42 45394.0 43 47045.5 44 50279.5 45 54114.0 46 56839.0 47 59667.5 48 62951.5 49 63883.0 50 63157.0 51 63259.5 52 64951.5 53 66622.0 54 67645.0 55 65506.5 56 61023.5 57 56346.0 58 53532.5 59 49923.0 60 43047.5 61 36756.0 62 30413.0 63 23289.5 64 17312.5 65 13200.0 66 9690.0 67 7697.0 68 6005.5 69 4270.0 70 3106.0 71 2058.5 72 1539.5 73 1314.0 74 991.0 75 713.5 76 477.5 77 283.5 78 179.0 79 94.0 80 44.0 81 23.0 82 20.0 83 12.5 84 7.0 85 4.5 86 1.5 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2445280960431122 2 0.01951522304959453 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.4085594238541854E-4 12-13 2.9390396159027906E-5 14-15 0.0 16-17 9.111022809298651E-4 18-19 0.0 20-21 2.9390396159027906E-5 22-23 2.9390396159027906E-5 24-25 0.002174889315768065 26-27 0.0024981836735173723 28-29 0.002263060504245149 30-31 9.992734694069488E-4 32-33 0.0013225678271562558 34-35 0.0014989102041104232 36-37 1.4695198079513953E-4 38-39 1.4695198079513953E-4 40-41 8.229310924527814E-4 42-43 0.0032329435774930697 44-45 0.004026484273786823 46-47 0.013313849460039643 48-49 0.013108116686926446 50-51 0.005995640816441693 52-53 0.006994914285848642 54-55 2.9390396159027906E-4 56-57 0.002821478031266679 58-59 0.0014107390156333395 60-61 0.0014107390156333395 62-63 0.0 64-65 7.053695078166698E-4 66-67 0.0 68-69 0.0 70-71 8.817118847708372E-5 72-73 9.40492677088893E-4 74-75 0.0 76-77 0.17834092389298134 78-79 0.09416682929352542 80-81 9.69883073247921E-4 82-83 0.0 84-85 4.996367347034744E-4 86-87 0.11415229868166439 88-89 0.559240458113983 90-91 0.29775410348711173 92-93 0.28567465066575126 94-95 0.00238062208888126 96-97 0.002615745258153484 98-99 0.0025863548619944558 100-101 9.992734694069488E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1701236.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.10035817242539 #Duplication Level Percentage of deduplicated Percentage of total 1 69.97729380115317 32.959556019713574 2 14.854947471846463 13.993466931130671 3 5.8525522707024145 8.269719245187758 4 2.897170100982299 5.458309977708326 5 1.6523129743495937 3.89122664524057 6 1.0790410755019535 3.049393268334067 7 0.7269742889768224 2.3968524575066827 8 0.5108913308575549 1.9250531736462333 9 0.36317934253988227 1.5395289403008983 >10 1.9011531537828747 16.07906463618528 >50 0.12766948165431435 4.157352965758343 >100 0.05394295202387094 4.58742320413543 >500 0.001872884105831197 0.6123782016930389 >1k 8.740125827212252E-4 0.7528729221237188 >5k 1.2485894038874646E-4 0.327801411335542 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5574 0.3276441363808431 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4180 0.24570371188947332 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2911 0.1711108864378605 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.878079231805581E-5 2 0.0 0.0 0.0 0.0 5.878079231805581E-5 3 0.0 0.0 0.0 0.0 5.878079231805581E-5 4 0.0 0.0 0.0 5.878079231805581E-5 5.878079231805581E-5 5 0.0 0.0 0.0 5.878079231805581E-5 5.878079231805581E-5 6 0.0 0.0 0.0 5.878079231805581E-5 5.878079231805581E-5 7 0.0 0.0 0.0 5.878079231805581E-5 1.1756158463611163E-4 8 0.0 0.0 0.0 5.878079231805581E-5 1.1756158463611163E-4 9 0.0 0.0 0.0 1.1756158463611163E-4 1.1756158463611163E-4 10-11 0.0 0.0 0.0 2.0573277311319534E-4 1.1756158463611163E-4 12-13 0.0 0.0 0.0 2.3512316927222325E-4 7.347599039756977E-4 14-15 0.0 0.0 0.0 2.3512316927222325E-4 0.0010874446578840325 16-17 0.0 0.0 0.0 2.9390396159027906E-4 0.0013225678271562558 18-19 0.0 0.0 0.0 2.9390396159027906E-4 0.0014107390156333395 20-21 0.0 0.0 0.0 4.408559423854186E-4 0.0017928141657007023 22-23 0.0 0.0 0.0 6.465887154986139E-4 0.001880985354177786 24-25 0.0 0.0 0.0 0.0011756158463611163 0.0019397661464958418 26-27 0.0 0.0 0.0 0.0014401294117923674 0.002086718127290981 28-29 0.0 0.0 0.0 0.001851594958018758 0.0021161085234500093 30-31 0.0 0.0 0.0 0.0034386763506062653 0.0021161085234500093 32-33 0.0 0.0 0.0 0.006054421608759749 0.0023806220888812606 34-35 0.0 0.0 0.0 0.008728947659231288 0.002468793277358344 36-37 0.0 0.0 0.0 0.014254342137128534 0.002468793277358344 38-39 0.0 0.0 0.0 0.025011227131332747 0.0025569644658354276 40-41 0.0 0.0 0.0 0.037384583914283495 0.0025863548619944558 42-43 0.0 0.0 0.0 0.04867049603935021 0.0026451356543125116 44-45 0.0 0.0 0.0 0.06310118055343292 0.0026745260504715393 46-47 0.0 0.0 0.0 0.08082358943732675 0.0027039164466305674 48-49 0.0 0.0 0.0 0.101102962787056 0.0029390396159027906 50-51 0.0 0.0 0.0 0.12487979327970958 0.0029978204082208464 52-53 0.0 0.0 0.0 0.14795125426454647 0.0030566012005389023 54-55 0.0 0.0 0.0 0.17237467347269866 0.003115381992856958 56-57 0.0 0.0 0.0 0.20311702785504188 0.003203553181334042 58-59 0.0 0.0 0.0 0.2378270857188538 0.0032917243698111255 60-61 0.0 0.0 0.0 0.2750941080485012 0.0033211147659701536 62-63 0.0 0.0 0.0 0.31820981921379515 0.003468066746765293 64-65 0.0 0.0 0.0 0.361266749586771 0.0036150187275604323 66-67 0.0 0.0 0.0 0.41102469028400523 0.0036444091237194604 68-69 0.0 0.0 0.0 0.47239183746405555 0.003703189916037516 70-71 0.0 0.0 0.0 0.5394607214989573 0.003703189916037516 72-73 0.0 0.0 0.0 0.6089690084150583 0.003997093877627795 74-75 0.0 0.0 0.0 0.68027010949686 0.004379169027695158 76-77 0.0 0.0 0.0 0.757184776245036 0.004408559423854186 78-79 0.0 2.9390396159027906E-5 0.0 0.8533501524773752 0.004467340216172242 80-81 0.0 5.878079231805581E-5 0.0 0.9614774199464389 0.004526121008490298 82-83 0.0 5.878079231805581E-5 0.0 1.0795386413172539 0.004878805762398633 84-85 0.0 5.878079231805581E-5 0.0 1.195248630995347 0.004878805762398633 86-87 0.0 5.878079231805581E-5 0.0 1.3178359733746525 0.004966976950875716 88-89 0.0 8.817118847708372E-5 0.0 1.4571170607722856 0.0050551481393528 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3030 0.0 51.18125 1 GTATCAA 5625 0.0 42.031006 1 ATCAACG 7210 0.0 32.372562 3 TCAACGC 7285 0.0 32.03928 4 TATCAAC 7345 0.0 31.849636 2 CAACGCA 7320 0.0 31.82128 5 AACGCAG 7670 0.0 30.307348 6 TACATGG 8000 0.0 26.09829 2 GTACATG 8360 0.0 25.719831 1 ACGCAGA 9235 0.0 25.017233 7 CGCAGAG 9345 0.0 24.621223 8 ACATGGG 8230 0.0 24.555965 3 GTATAGG 395 0.0 24.08623 1 TGGTATC 1525 0.0 23.959084 2 GTGGTAT 1670 0.0 22.788168 1 CATGGGG 4870 0.0 22.502472 4 TACCTGG 1255 0.0 22.307844 2 GCAGAGT 10845 0.0 20.99708 9 GTACCTG 1415 0.0 20.171154 1 GAGTACA 6265 0.0 20.045588 1 >>END_MODULE