FastQCFastQC Report
Thu 26 May 2016
SRR936284_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936284_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830654
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59580.3254574594653058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43190.2359266142045411No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29000.15841333206602667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40300.039.7197071
GTATCAA67950.038.053761
ATCAACG82700.031.141073
TCAACGC83000.031.0285114
CAACGCA84400.030.5699145
GTACATG89600.030.5502131
AACGCAG85950.030.0178056
GTACCTG15750.029.7679581
TATCAAC86550.029.7550092
TACATGG89850.029.7159292
ACATGGG90900.028.3839473
TACCTGG16450.027.9147742
ACGCAGA101950.025.1675157
CGCAGAG102750.024.9729278
CATGGGG38500.024.592884
CATGGGA53800.023.4066644
GCAGAGT110900.022.6680959
GGACCGT2205.8489604E-821.5181856
CGCGGAA4900.021.35591182-83
CTCGCGC4500.021.13758560-61
Produced by FastQC (version 0.11.5)