##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936284_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1830654 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02106460314183 33.0 31.0 34.0 30.0 34.0 2 32.118113526641295 34.0 31.0 34.0 30.0 34.0 3 32.18626567336045 34.0 31.0 34.0 30.0 34.0 4 35.678910378476765 37.0 35.0 37.0 35.0 37.0 5 35.679808964446586 37.0 35.0 37.0 35.0 37.0 6 35.77380651941874 37.0 35.0 37.0 35.0 37.0 7 35.72461535604216 37.0 35.0 37.0 35.0 37.0 8 35.729757780552745 37.0 35.0 37.0 35.0 37.0 9 37.43501775868078 39.0 38.0 39.0 35.0 39.0 10-11 37.367755184759105 39.0 37.5 39.0 35.0 39.0 12-13 37.2875049026195 39.0 37.0 39.0 35.0 39.0 14-15 38.57415928952167 40.0 38.0 41.0 34.5 41.0 16-17 38.53039050525113 40.0 38.0 41.0 34.0 41.0 18-19 38.4991142509726 40.0 38.0 41.0 34.0 41.0 20-21 38.438683115433065 40.0 38.0 41.0 34.0 41.0 22-23 38.28880635008035 40.0 38.0 41.0 34.0 41.0 24-25 38.44246318528788 40.0 38.0 41.0 34.0 41.0 26-27 38.40199731899092 40.0 38.0 41.0 34.0 41.0 28-29 38.28437296179398 40.0 38.0 41.0 34.0 41.0 30-31 38.13804684009103 40.0 38.0 41.0 34.0 41.0 32-33 37.965108097980284 40.0 38.0 41.0 33.5 41.0 34-35 37.71165796485846 40.0 38.0 41.0 33.0 41.0 36-37 37.51824593833679 40.0 37.5 41.0 33.0 41.0 38-39 37.37309371404973 40.0 37.0 41.0 32.5 41.0 40-41 37.1877897188655 40.0 37.0 41.0 32.0 41.0 42-43 37.00610929208906 40.0 37.0 41.0 31.0 41.0 44-45 36.814536225851526 40.0 37.0 41.0 31.0 41.0 46-47 36.50935157599415 40.0 36.0 41.0 30.0 41.0 48-49 36.3237758200075 40.0 36.0 41.0 30.0 41.0 50-51 36.00574029827591 39.5 35.5 40.5 29.5 41.0 52-53 36.02315839038945 39.0 36.0 40.5 30.0 41.0 54-55 36.099156913321686 39.0 35.5 41.0 29.5 41.0 56-57 35.9557562488597 39.0 35.0 41.0 29.0 41.0 58-59 35.74829214040447 39.0 35.0 41.0 28.5 41.0 60-61 35.47829027221965 39.0 35.0 41.0 28.0 41.0 62-63 35.19027708130537 38.0 35.0 40.5 28.0 41.0 64-65 34.87100019992855 37.5 35.0 40.0 28.0 41.0 66-67 34.53132842142753 37.0 34.0 40.0 27.0 41.0 68-69 34.165059317599066 36.5 34.0 39.5 26.0 41.0 70-71 33.76107937381941 36.0 34.0 39.0 26.0 41.0 72-73 33.362368038963126 35.5 34.0 39.0 26.0 40.0 74-75 32.94879889919122 35.0 33.5 37.5 25.0 39.5 76-77 32.55271012435993 35.0 33.0 37.0 24.5 39.0 78-79 32.16433580567382 35.0 33.0 36.5 23.5 39.0 80-81 31.774964848627867 35.0 32.5 36.0 22.5 38.0 82-83 31.437484636638054 35.0 32.0 36.0 20.5 37.0 84-85 31.128842752371558 35.0 32.0 35.0 20.0 37.0 86-87 30.824031466350277 35.0 32.0 35.0 18.5 36.0 88-89 30.5273074540574 34.5 31.5 35.0 16.5 36.0 90-91 30.204469550226314 34.0 31.0 35.0 9.0 36.0 92-93 29.927762154945718 34.0 31.0 35.0 4.5 35.0 94-95 29.584676842265115 34.0 31.0 35.0 2.0 35.0 96-97 29.211083033713635 34.0 30.0 35.0 2.0 35.0 98-99 28.77042548728487 34.0 30.0 35.0 2.0 35.0 100-101 25.573347011505177 30.5 24.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15007633608090032 1101 2 0.045846167626862666 1101 3 0.08172071119245317 1101 4 -0.04677648388361888 1101 5 0.19300571886962103 1101 6 0.08349371871811684 1101 7 0.16543801753551435 1101 8 0.15104553992311764 1101 9 0.07736147248005665 1101 10-11 0.24534746073418034 1101 12-13 0.17498061711611257 1101 14-15 0.3565297361735773 1101 16-17 0.267237821925157 1101 18-19 0.3435998265520581 1101 20-21 0.3748117891827647 1101 22-23 0.32110203625232714 1101 24-25 0.2889202451552393 1101 26-27 0.2780077677819719 1101 28-29 0.25977563089275435 1101 30-31 0.29076764562620383 1101 32-33 0.36324562696925966 1101 34-35 0.21244673843890638 1101 36-37 0.16277053653214324 1101 38-39 0.18759282898395213 1101 40-41 0.19970663777177577 1101 42-43 0.1973841666343219 1101 44-45 0.34074857096838684 1101 46-47 0.30405138911255136 1101 48-49 0.16108740164187196 1101 50-51 0.30923942228543666 1101 52-53 0.43577117384246833 1101 54-55 0.35007365804305124 1101 56-57 0.3329363458934722 1101 58-59 0.4628210563871278 1101 60-61 0.3570394694229506 1101 62-63 0.22218556618181395 1101 64-65 0.17914065749410213 1101 66-67 0.13137297882934718 1101 68-69 0.22251516828199414 1101 70-71 0.3140980554505859 1101 72-73 0.3251133528772101 1101 74-75 0.13729023228923154 1101 76-77 0.1420107343971253 1101 78-79 0.11423684308244475 1101 80-81 0.207116647735063 1101 82-83 0.37617332435832296 1101 84-85 0.25235936088552435 1101 86-87 0.3002782972238407 1101 88-89 0.25358855210575726 1101 90-91 0.3543020868604607 1101 92-93 0.39243090905514677 1101 94-95 0.5106599811135482 1101 96-97 0.5117443728061026 1101 98-99 0.5498706246973057 1101 100-101 0.6274924166993046 1102 1 0.21152885004737954 1102 2 0.12033406334381169 1102 3 0.1977976820180274 1102 4 0.07985293883519518 1102 5 0.16755568783299424 1102 6 0.19274263243629974 1102 7 0.15488556564228162 1102 8 0.05545274041970316 1102 9 0.23440678594995035 1102 10-11 0.34125062646975124 1102 12-13 0.306886265781543 1102 14-15 0.38973892301404334 1102 16-17 0.25358170771038857 1102 18-19 0.32218455653344336 1102 20-21 0.1861091200331657 1102 22-23 0.28572028578678044 1102 24-25 0.4081008783023492 1102 26-27 0.3074925598365965 1102 28-29 0.21135849867673784 1102 30-31 0.27524933401850404 1102 32-33 0.07739832715547834 1102 34-35 0.08985207673810436 1102 36-37 0.10597351604797467 1102 38-39 0.34867290347184365 1102 40-41 0.1844986923962324 1102 42-43 0.09527367625567251 1102 44-45 -0.004378679186793022 1102 46-47 0.09548528110509835 1102 48-49 -0.010855490971415804 1102 50-51 -0.00950669813666849 1102 52-53 0.017397492898957978 1102 54-55 0.22282350285993857 1102 56-57 0.06384882261600211 1102 58-59 0.09069194403455327 1102 60-61 0.19254536638135988 1102 62-63 0.40157724836679165 1102 64-65 0.1490351329751718 1102 66-67 0.31045429478217557 1102 68-69 0.31562503792817864 1102 70-71 0.3277541696653614 1102 72-73 0.38687623307584573 1102 74-75 0.3762722310477642 1102 76-77 0.27764077784839714 1102 78-79 0.27779984742757335 1102 80-81 0.22387642427138132 1102 82-83 0.2551304938182888 1102 84-85 0.2514282621890622 1102 86-87 0.3067959880990756 1102 88-89 0.3712173540921029 1102 90-91 0.43748023707771466 1102 92-93 0.44829683084285676 1102 94-95 0.4153775478422901 1102 96-97 0.5573682089327328 1102 98-99 0.5359041442503774 1102 100-101 0.7773993068296576 1103 1 0.23285299287989858 1103 2 0.22763384662124508 1103 3 0.2709670931762602 1103 4 0.25554767877574136 1103 5 0.20149160445047443 1103 6 0.17450308766044031 1103 7 0.2720225095948692 1103 8 0.2681371109253021 1103 9 0.32783235982245174 1103 10-11 0.3354059041445794 1103 12-13 0.3885558607192934 1103 14-15 0.38029906161887794 1103 16-17 0.5528365237249844 1103 18-19 0.5129326306613962 1103 20-21 0.43944155353320014 1103 22-23 0.5401660723554755 1103 24-25 0.5420138034163955 1103 26-27 0.5676215944069725 1103 28-29 0.5400401513807367 1103 30-31 0.5522260313862617 1103 32-33 0.5178947529870968 1103 34-35 0.21965722469968085 1103 36-37 0.23505231551783368 1103 38-39 0.3261745925657138 1103 40-41 0.38158144045663533 1103 42-43 0.2426251923222722 1103 44-45 0.31559784889029174 1103 46-47 0.3592664568974513 1103 48-49 0.46010353306024143 1103 50-51 0.39771076564505137 1103 52-53 0.44839819903932465 1103 54-55 0.38841246663326245 1103 56-57 0.40483016708896713 1103 58-59 0.43786574302399117 1103 60-61 0.3834488142680428 1103 62-63 0.44768112268295823 1103 64-65 0.3483352456831028 1103 66-67 0.3584563979518691 1103 68-69 0.3911479325851488 1103 70-71 0.4130185223878158 1103 72-73 0.5412521681780404 1103 74-75 0.5038424752381161 1103 76-77 0.4497053004715781 1103 78-79 0.4818898566851786 1103 80-81 0.7292775211529232 1103 82-83 0.7132613646586528 1103 84-85 0.5217745988761813 1103 86-87 0.5500054875934879 1103 88-89 0.36377657151165366 1103 90-91 0.534607519260863 1103 92-93 0.532913379844878 1103 94-95 0.46546337516275216 1103 96-97 0.7163372973710942 1103 98-99 0.9379115350845879 1103 100-101 0.9058886392296017 1104 1 0.327202849887982 1104 2 0.2323375475274787 1104 3 0.29880645645866366 1104 4 0.34590583242503214 1104 5 0.3009772482399171 1104 6 0.17096087209291255 1104 7 0.2156432633422014 1104 8 0.2755714316507607 1104 9 0.3009475101527883 1104 10-11 0.3362252752171102 1104 12-13 0.307931857626933 1104 14-15 0.44933653571964527 1104 16-17 0.5188005972839917 1104 18-19 0.4280710758631372 1104 20-21 0.555446058778216 1104 22-23 0.5072538653926273 1104 24-25 0.4238790444588503 1104 26-27 0.3995226019408875 1104 28-29 0.5409865194157106 1104 30-31 0.4281077901794177 1104 32-33 0.469879719212571 1104 34-35 0.5090625679024896 1104 36-37 0.44800215504994867 1104 38-39 0.3760597769445724 1104 40-41 0.48577282154675316 1104 42-43 0.5118654172587043 1104 44-45 0.45815175410242404 1104 46-47 0.5248160686495922 1104 48-49 0.5824367819757654 1104 50-51 0.5453669496936371 1104 52-53 0.5863128274602829 1104 54-55 0.49580098699646413 1104 56-57 0.5449198499001682 1104 58-59 0.4953314982745596 1104 60-61 0.4074501294086019 1104 62-63 0.5381670138017611 1104 64-65 0.45528297921311633 1104 66-67 0.5657667207685222 1104 68-69 0.49522157305119663 1104 70-71 0.3237261435384724 1104 72-73 0.5022381829615199 1104 74-75 0.42067705188281934 1104 76-77 0.5012627349404042 1104 78-79 0.3629294323450054 1104 80-81 0.40471049563652883 1104 82-83 0.5093473775168214 1104 84-85 0.37027888567160616 1104 86-87 0.38688899694535195 1104 88-89 0.29828353449963174 1104 90-91 0.5697680908138416 1104 92-93 0.5296986535288895 1104 94-95 0.3073538230658599 1104 96-97 0.5425463973143749 1104 98-99 0.6824121593320456 1104 100-101 0.9226203964676927 1105 1 0.20197155232962416 1105 2 0.174556200403444 1105 3 0.26380749553766236 1105 4 0.12182194185663064 1105 5 0.12768418055434694 1105 6 0.1663914197321006 1105 7 0.15843082533493913 1105 8 0.18277080542298307 1105 9 0.2142915301373236 1105 10-11 0.26354802669829525 1105 12-13 0.3369815120574202 1105 14-15 0.27745076953345205 1105 16-17 0.34524161092604544 1105 18-19 0.3458054273869493 1105 20-21 0.40661964770715286 1105 22-23 0.40648138234316633 1105 24-25 0.38294414427281254 1105 26-27 0.3930216746118944 1105 28-29 0.25990264061046275 1105 30-31 0.44693490347538756 1105 32-33 0.3535925213430531 1105 34-35 0.2088844095211826 1105 36-37 0.28606962496210997 1105 38-39 0.45879987281074364 1105 40-41 0.4044018860298877 1105 42-43 0.43158916839446704 1105 44-45 0.34180502752206365 1105 46-47 0.4733285459743186 1105 48-49 0.26765020666250194 1105 50-51 0.22441344945890762 1105 52-53 0.2668855079169319 1105 54-55 0.38029407863610487 1105 56-57 0.472479646846665 1105 58-59 0.40352165079995217 1105 60-61 0.3469185201978391 1105 62-63 0.29128986502026066 1105 64-65 0.2806783193626643 1105 66-67 0.2989850603167241 1105 68-69 0.27743328922828425 1105 70-71 0.29031823890988306 1105 72-73 0.3077174265120135 1105 74-75 0.3459820652340113 1105 76-77 0.41955761878634235 1105 78-79 0.38348679930814455 1105 80-81 0.41912945442590654 1105 82-83 0.4428358063117521 1105 84-85 0.3530182329731595 1105 86-87 0.2823275292738998 1105 88-89 0.2719691708633327 1105 90-91 0.45587719090013934 1105 92-93 0.4062562472001616 1105 94-95 0.33641898347791255 1105 96-97 0.5225866344453891 1105 98-99 0.5023508198077238 1105 100-101 0.702711535993366 1106 1 0.24262987516557644 1106 2 0.221671367036258 1106 3 0.2848737846913991 1106 4 0.1962224471175702 1106 5 0.15323008491111523 1106 6 0.12750823497977848 1106 7 0.22844600118834535 1106 8 0.12412954692945988 1106 9 0.13914436456938972 1106 10-11 0.15283449120973813 1106 12-13 0.16069012596609866 1106 14-15 0.27557545279210416 1106 16-17 0.23060914874262295 1106 18-19 0.27862194514906946 1106 20-21 0.2626691560691228 1106 22-23 0.3221517508827674 1106 24-25 0.24293892389016492 1106 26-27 0.3207349736926801 1106 28-29 0.2800746012627826 1106 30-31 0.26946407834295627 1106 32-33 0.2267734467720004 1106 34-35 0.09733998045980741 1106 36-37 0.23933766769298614 1106 38-39 0.2010420930193817 1106 40-41 0.08898175615120607 1106 42-43 0.25348295405927956 1106 44-45 0.36756359487713297 1106 46-47 0.37694506484569246 1106 48-49 0.38387668807606445 1106 50-51 0.35721811216880184 1106 52-53 0.3827367526627583 1106 54-55 0.40196594604254443 1106 56-57 0.5490627642817003 1106 58-59 0.49279868673185234 1106 60-61 0.5464563811443099 1106 62-63 0.4677252795969835 1106 64-65 0.5198976745726114 1106 66-67 0.5507827299323651 1106 68-69 0.5005216273926081 1106 70-71 0.616888877342582 1106 72-73 0.6164046146669477 1106 74-75 0.4851272743716919 1106 76-77 0.6277890113806066 1106 78-79 0.5544380389930339 1106 80-81 0.6796496000419978 1106 82-83 0.6488799071060392 1106 84-85 0.5496581922638804 1106 86-87 0.5819591213935489 1106 88-89 0.5605218353729775 1106 90-91 0.56006507475945 1106 92-93 0.4793738416922082 1106 94-95 0.42483462160902974 1106 96-97 0.5187729126730645 1106 98-99 0.7733002916339515 1106 100-101 1.0023720724656755 1107 1 -0.20258481321604194 1107 2 -0.020809378055979266 1107 3 0.08985037732463041 1107 4 0.014718017470762845 1107 5 0.04992404886098001 1107 6 0.082773124358269 1107 7 0.015944759915655027 1107 8 0.13329281840276508 1107 9 -0.02519442464857491 1107 10-11 0.031935781204616376 1107 12-13 0.07617124326154112 1107 14-15 0.1315017351250276 1107 16-17 -0.13665361457685776 1107 18-19 -0.06570174162013842 1107 20-21 0.0940653896012762 1107 22-23 0.02300860410110772 1107 24-25 0.18479038837762118 1107 26-27 0.09690368104737246 1107 28-29 0.16887407089107853 1107 30-31 0.16989900600622576 1107 32-33 0.21790968814828204 1107 34-35 0.1462087970464907 1107 36-37 0.1479926781839751 1107 38-39 0.32145429556936733 1107 40-41 0.3181876702529891 1107 42-43 0.012635688109732257 1107 44-45 0.32059008560099045 1107 46-47 0.49918720696445007 1107 48-49 0.27093605413041644 1107 50-51 0.27729836803886343 1107 52-53 0.3472384968741338 1107 54-55 0.4121400957082315 1107 56-57 0.28661574608661056 1107 58-59 0.4203678354438196 1107 60-61 0.43779483387914553 1107 62-63 0.45958395954009035 1107 64-65 0.33682656232798536 1107 66-67 0.34414690398058667 1107 68-69 0.37719216611566964 1107 70-71 0.37993938624651946 1107 72-73 0.20154163331211095 1107 74-75 0.09312460679630163 1107 76-77 -0.007403087624012983 1107 78-79 0.0934343619682565 1107 80-81 0.2429211090652501 1107 82-83 0.41610162805650575 1107 84-85 0.3248488838974488 1107 86-87 -0.05572141680800513 1107 88-89 0.15065930766312974 1107 90-91 0.26657284423349736 1107 92-93 0.006624688793849032 1107 94-95 0.00849479365120942 1107 96-97 0.09420192866351584 1107 98-99 0.13795959710705574 1107 100-101 0.08434697125283463 1108 1 -0.11632327862234604 1108 2 -0.040166906748268616 1108 3 -0.08581066003612392 1108 4 -0.12105782007084542 1108 5 -0.05506700694684241 1108 6 -0.14239704290692146 1108 7 -0.07681204806821285 1108 8 -0.021612933918120802 1108 9 -0.08207824533433694 1108 10-11 -0.08509087755764 1108 12-13 -0.14457804104789318 1108 14-15 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2316 72-73 -0.9543771816363318 2316 74-75 -0.9130775585321373 2316 76-77 -1.239442217090037 2316 78-79 -1.3193270089590001 2316 80-81 -1.1241003160354879 2316 82-83 -1.1895281990339903 2316 84-85 -1.2659439065736002 2316 86-87 -1.2786329325647543 2316 88-89 -1.3880948633994272 2316 90-91 -1.4358237550250372 2316 92-93 -1.2857655213230075 2316 94-95 -1.270404260594752 2316 96-97 -1.4445674098992498 2316 98-99 -1.2525142731826335 2316 100-101 -1.3473211584735871 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10019.0 3 2289.0 4 1897.0 5 1619.0 6 1541.0 7 1635.0 8 1896.0 9 2298.0 10 2847.0 11 3587.0 12 5137.0 13 8182.0 14 8910.0 15 7811.0 16 9616.0 17 13104.0 18 12072.0 19 7759.0 20 6935.0 21 7048.0 22 7388.0 23 7980.0 24 9147.0 25 10509.0 26 12700.0 27 15355.0 28 18568.0 29 22912.0 30 28967.0 31 36470.0 32 47864.0 33 64123.0 34 92769.0 35 141831.0 36 237710.0 37 411749.0 38 455023.0 39 93258.0 40 129.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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22.576826888010785 18-19 22.769779348236117 26.5191029485155 28.395977303016423 22.315140400231964 20-21 23.507364949075342 25.75024042709576 28.481972952532136 22.26042167129676 22-23 23.032086614657153 25.853394580765844 28.27606796727777 22.838450837299238 24-25 22.9365108851127 25.998525923101045 28.518661988375666 22.546301203410593 26-27 22.78642340782712 26.471361073523397 28.308476274396583 22.433739244252905 28-29 22.42432238850982 26.2527979422661 28.669941426919145 22.65293824230494 30-31 22.49946118643878 26.218790812224906 28.665154619687772 22.616593381648542 32-33 22.452715203495146 26.31807539726441 28.566460949359012 22.662748449881434 34-35 23.072291333977354 26.276595977717758 28.160603232809134 22.49050945549575 36-37 23.050759942297173 26.225375746046097 27.98858783357047 22.73527647808626 38-39 22.72438953995604 26.398535400701682 28.28113700137944 22.59593805796284 40-41 22.867319938435752 26.129187780556222 28.45955202104753 22.543940259960493 42-43 22.806451462438858 26.205204312864595 28.15892747441373 22.829416750282814 44-45 22.782761412126284 26.538616687018923 28.002497152971202 22.676124747883595 46-47 22.71461773606311 26.660572478621177 27.543223499818463 23.08158628549725 48-49 23.133311670205224 26.694252391543476 27.366294221872877 22.806141716378423 50-51 23.015534063723806 26.738840209609403 27.19059502413714 23.055030702529653 52-53 23.233502235763932 26.772823715097836 27.218082833455142 22.77559121568309 54-55 22.871809844898976 26.540493356321832 27.341203317873813 23.246493480905375 56-57 22.90671267865042 27.144381258458488 27.097128970756266 22.851777092134824 58-59 23.136520055285565 26.580809587221427 27.33413987840359 22.948530479089417 60-61 23.174104661123682 26.672951960852643 27.544131051676423 22.608812326347255 62-63 23.05825236058662 26.69459703957071 27.46778124750483 22.77936935233784 64-65 22.815254497755237 26.49973655613001 27.507766105750758 23.17724284036399 66-67 22.814416978038064 27.020269708581612 27.11837947684118 23.04693383653914 68-69 22.758394904934036 26.754310377947725 27.483509861152427 23.003784855965815 70-71 22.697561477518263 26.819467023356314 27.449573001337583 23.03339849778784 72-73 22.889338352619387 26.822036235337894 27.23942285344711 23.04920255859561 74-75 22.71678946536462 26.942806396655893 27.12692211430378 23.213482023675706 76-77 22.913485907338238 26.931889401225146 27.164526159698905 22.99009853173771 78-79 22.875689659477587 26.91356066657319 27.143037852073785 23.067711821875434 80-81 22.60700651763978 27.466341626182956 27.077264435287894 22.849387420889375 82-83 23.03859719986587 27.268653611960087 26.710130523284054 22.98261866488999 84-85 22.932988894367934 26.88690234612 26.764707738653364 23.4154010208587 86-87 22.811010968819048 26.765268589530393 27.262915776197893 23.160804665452666 88-89 22.95240695939984 26.926109550581074 27.0302343356556 23.091249154363485 90-91 22.85135624425765 27.168504355469324 27.13515172427613 22.844987675996894 92-93 22.88067718640467 26.836832440829905 27.179021274610175 23.103469098155248 94-95 22.80655442607611 26.821037496003985 27.067617386818434 23.304790691101473 96-97 22.891168648898365 26.80969972166274 27.027711374189415 23.27142025524948 98-99 22.759924717505484 26.68877447593163 26.93620491629472 23.615095890268165 100-101 22.75294654048453 26.807558528226245 26.87732137022671 23.56217356106251 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 192.0 1 138.5 2 188.0 3 339.0 4 296.5 5 255.0 6 549.0 7 1133.0 8 1760.0 9 2008.0 10 1777.0 11 1642.0 12 1776.0 13 2040.5 14 2295.5 15 2397.5 16 2580.5 17 2738.5 18 2755.5 19 2850.5 20 2999.0 21 3213.0 22 3585.0 23 4133.0 24 4849.5 25 5863.5 26 7559.0 27 9697.5 28 11562.0 29 13652.5 30 16362.5 31 19576.5 32 23515.0 33 27425.0 34 31322.5 35 35207.0 36 39918.0 37 44623.5 38 47937.0 39 50233.0 40 51089.0 41 53989.5 42 58299.0 43 61696.5 44 64625.0 45 66988.5 46 66812.0 47 66247.5 48 71621.5 49 73388.5 50 69860.0 51 67630.0 52 66397.0 53 67105.0 54 66648.5 55 62138.5 56 55389.0 57 49249.5 58 44732.0 59 40180.0 60 35129.0 61 29723.5 62 24802.0 63 20103.5 64 16014.5 65 13016.5 66 9406.5 67 7288.0 68 5071.5 69 2760.5 70 2133.5 71 1812.5 72 1520.5 73 1364.5 74 1102.5 75 887.0 76 586.5 77 289.0 78 166.5 79 109.0 80 75.0 81 50.5 82 37.5 83 27.0 84 22.5 85 21.0 86 14.5 87 15.5 88 16.0 89 15.5 90 16.0 91 13.0 92 15.0 93 15.5 94 12.0 95 9.0 96 13.0 97 17.0 98 11.0 99 6.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04337247781393972 2 0.010925057383863908 3 0.009286298776284322 4 0.005407903405012635 5 0.007811416029462694 6 0.00480702524890012 7 0.004424648240464883 8 0.00835766889865589 9 0.012946192999878732 10-11 0.0020757609029341427 12-13 0.007866041316382015 14-15 0.007756790742543375 16-17 0.017562029744561232 18-19 0.010406117158130373 20-21 0.003140953997860874 22-23 0.008494232115954188 24-25 0.019446602143277758 26-27 0.0066096597172376655 28-29 0.01589595849352199 30-31 0.013301257364854309 32-33 0.012536503347983834 34-35 0.2526965772887722 36-37 0.4486374814683714 38-39 0.4466163458523566 40-41 0.46390524916232123 42-43 0.455492954976746 44-45 0.46863033648084235 46-47 0.47521268355462043 48-49 0.48168578005455975 50-51 0.4905350765354895 52-53 0.4803474605250364 54-55 0.4688761502719793 56-57 0.4654074445526025 58-59 0.46314049514545075 60-61 0.45945328827839665 62-63 0.45800571817503466 64-65 0.47163472726140493 66-67 0.44822779181647654 68-69 0.47283648357363 70-71 0.45565683083750397 72-73 0.46335899629312804 74-75 0.4753492467719187 76-77 0.4643149388142161 78-79 0.46259424227625756 80-81 0.46551669512644117 82-83 0.46578982156103776 84-85 0.46761976867283495 86-87 0.46868496176776175 88-89 0.48078446282039095 90-91 0.503016954596554 92-93 0.4915729569869565 94-95 0.4657625089175781 96-97 0.43946043326592577 98-99 0.4326049597575511 100-101 0.43976087234398203 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1830654.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.7902523316863 #Duplication Level Percentage of deduplicated Percentage of total 1 72.34081245073767 35.29526493350685 2 14.381843003660313 14.033874982865676 3 5.428732819655516 7.946077323368985 4 2.6446053222067465 5.161238439527508 5 1.4536569632058376 3.5462145019262814 6 0.8699451755755846 2.5466906778639538 7 0.565229716589875 1.9304390348451268 8 0.3947217639501407 1.5406859571148543 9 0.30341020791625617 1.3323114543819188 >10 1.4076446800673172 12.407293689158934 >50 0.12188798958873026 4.117951296915972 >100 0.08167650721330796 7.190903616384679 >500 0.004375049724460258 1.5144697726040786 >1k 0.0013461691459877717 1.110483455596825 >5k 1.1218076216564764E-4 0.32610086393838456 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5958 0.3254574594653058 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4319 0.2359266142045411 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2900 0.15841333206602667 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.731264345965977E-5 0.0 18-19 0.0 0.0 0.0 8.193793037897931E-5 0.0 20-21 0.0 5.462528691931954E-5 0.0 2.1850114767727816E-4 0.0 22-23 0.0 5.462528691931954E-5 0.0 4.096896518948966E-4 0.0 24-25 0.0 5.462528691931954E-5 0.0 7.101287299511541E-4 0.0 26-27 0.0 5.462528691931954E-5 0.0 8.466919472494529E-4 0.0 28-29 0.0 5.462528691931954E-5 0.0 0.0012017563122250301 0.0 30-31 0.0 8.193793037897931E-5 0.0 0.002977078137102915 0.0 32-33 0.0 1.0925057383863908E-4 0.0 0.006172657421883108 0.0 34-35 0.0 1.0925057383863908E-4 0.0 0.010460742445049694 0.0 36-37 0.0 1.0925057383863908E-4 0.0 0.0191461630652215 0.0 38-39 0.0 1.0925057383863908E-4 0.0 0.03222891928239853 0.0 40-41 0.0 1.0925057383863908E-4 0.0 0.04700505939407447 0.0 42-43 0.0 1.0925057383863908E-4 0.0 0.060661381123904345 0.0 44-45 0.0 1.0925057383863908E-4 0.0 0.0782780361553849 0.0 46-47 0.0 1.0925057383863908E-4 0.0 0.09966383598429851 0.0 48-49 0.0 1.0925057383863908E-4 0.0 0.1245183415325889 0.0 50-51 0.0 1.0925057383863908E-4 0.0 0.15396137118210212 0.0 52-53 0.0 1.0925057383863908E-4 0.0 0.18315858704047844 0.0 54-55 0.0 1.6387586075795863E-4 0.0 0.2123011776119354 0.0 56-57 0.0 1.6387586075795863E-4 0.0 0.25010187616010454 0.0 58-59 0.0 2.458137911369379E-4 0.0 0.29598711717233295 0.0 60-61 0.0 2.731264345965977E-4 0.0 0.34681594665075977 0.0 62-63 0.0 2.731264345965977E-4 0.0 0.3986007186502747 0.0 64-65 0.0 2.731264345965977E-4 0.0 0.451559934318555 0.0 66-67 0.0 2.731264345965977E-4 0.0 0.5117296878601855 0.0 68-69 0.0 2.731264345965977E-4 0.0 0.580093234439714 0.0 70-71 0.0 2.731264345965977E-4 0.0 0.6623042912532897 0.0 72-73 0.0 2.731264345965977E-4 0.0 0.7505514422714505 0.0 74-75 0.0 2.731264345965977E-4 0.0 0.8361492668740242 0.0 76-77 0.0 2.731264345965977E-4 0.0 0.9307875764617454 0.0 78-79 0.0 2.731264345965977E-4 0.0 1.0457191801399937 0.0 80-81 0.0 2.731264345965977E-4 0.0 1.1788410043623754 0.0 82-83 0.0 2.731264345965977E-4 0.0 1.3133830860446594 0.0 84-85 0.0 2.731264345965977E-4 0.0 1.449318112543386 0.0 86-87 0.0 2.731264345965977E-4 0.0 1.5961508837825171 0.0 88-89 0.0 2.731264345965977E-4 0.0 1.7660628387450603 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4030 0.0 39.719707 1 GTATCAA 6795 0.0 38.05376 1 ATCAACG 8270 0.0 31.14107 3 TCAACGC 8300 0.0 31.028511 4 CAACGCA 8440 0.0 30.569914 5 GTACATG 8960 0.0 30.550213 1 AACGCAG 8595 0.0 30.017805 6 GTACCTG 1575 0.0 29.767958 1 TATCAAC 8655 0.0 29.755009 2 TACATGG 8985 0.0 29.715929 2 ACATGGG 9090 0.0 28.383947 3 TACCTGG 1645 0.0 27.914774 2 ACGCAGA 10195 0.0 25.167515 7 CGCAGAG 10275 0.0 24.972927 8 CATGGGG 3850 0.0 24.59288 4 CATGGGA 5380 0.0 23.406664 4 GCAGAGT 11090 0.0 22.668095 9 GGACCGT 220 5.8489604E-8 21.518185 6 CGCGGAA 490 0.0 21.355911 82-83 CTCGCGC 450 0.0 21.137585 60-61 >>END_MODULE