FastQCFastQC Report
Thu 26 May 2016
SRR936284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936284_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830654
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63090.344630935173987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45850.2504569405250801No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32890.179662568677642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69200.036.2262761
GGTATCA43500.034.8835451
GTACATG87050.030.819781
TACATGG87150.030.3795382
ATCAACG85300.029.1964283
TCAACGC85550.029.1111154
CAACGCA87800.028.4191255
ACATGGG88150.028.198663
TATCAAC90000.027.7832162
AACGCAG90150.027.6256836
GTACCTG16450.026.0253051
TACCTGG16150.024.972432
CATGGGG39000.024.570064
ACGCAGA105600.023.5389397
CGCAGAG107300.022.944958
CATGGGA50950.022.0660044
GTATAGG6500.021.954681
AGTACAT59200.021.8002552
GCAGAGT115200.021.12449
TATCACG1150.003747991820.6293982
Produced by FastQC (version 0.11.5)