##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936284_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1830654 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39679371415898 34.0 31.0 34.0 31.0 34.0 2 32.53252171082028 34.0 31.0 34.0 31.0 34.0 3 32.61241119293979 34.0 31.0 34.0 31.0 34.0 4 36.12540436368642 37.0 35.0 37.0 35.0 37.0 5 36.08348054848158 37.0 35.0 37.0 35.0 37.0 6 36.12842459580019 37.0 36.0 37.0 35.0 37.0 7 36.07340764557366 37.0 35.0 37.0 35.0 37.0 8 36.05292207047317 37.0 35.0 37.0 35.0 37.0 9 37.80613485672333 39.0 38.0 39.0 35.0 39.0 10-11 37.73218860582065 39.0 37.5 39.0 35.0 39.0 12-13 37.67259269091811 39.0 37.0 39.0 35.0 39.0 14-15 38.9757493769986 40.0 38.0 41.0 35.5 41.0 16-17 38.89966673112451 40.0 38.0 41.0 35.0 41.0 18-19 38.930177138880424 40.0 38.0 41.0 35.0 41.0 20-21 38.88921636748397 40.0 38.0 41.0 35.0 41.0 22-23 38.765212869280596 40.0 38.0 41.0 34.5 41.0 24-25 38.92444011812172 40.0 38.0 41.0 35.0 41.0 26-27 38.84267016050002 40.0 38.0 41.0 35.0 41.0 28-29 38.74076914588994 40.0 38.0 41.0 35.0 41.0 30-31 38.609666272272094 40.0 38.0 41.0 34.0 41.0 32-33 38.44655871617466 40.0 38.0 41.0 34.0 41.0 34-35 38.290092229334434 40.0 38.0 41.0 34.0 41.0 36-37 38.12855924713244 40.0 38.0 41.0 33.5 41.0 38-39 37.99410156151845 40.0 37.5 41.0 33.0 41.0 40-41 37.834300474038244 40.0 37.0 41.0 33.0 41.0 42-43 37.842554354891746 40.0 37.0 41.0 33.0 41.0 44-45 37.687961788519296 40.0 37.0 41.0 33.0 41.0 46-47 37.440606198659054 40.0 37.0 41.0 32.5 41.0 48-49 37.17741555750021 40.0 37.0 41.0 32.0 41.0 50-51 36.96584417372152 40.0 36.5 41.0 31.0 41.0 52-53 36.743449608719075 40.0 36.0 41.0 31.0 41.0 54-55 36.53728722085113 39.0 35.5 41.0 31.0 41.0 56-57 36.295411093521764 39.0 35.0 41.0 30.0 41.0 58-59 36.03806453868399 39.0 35.0 41.0 29.5 41.0 60-61 35.761326553242725 38.0 35.0 40.0 29.0 41.0 62-63 35.4696373536452 38.0 35.0 40.0 29.0 41.0 64-65 35.09619267212701 37.0 34.0 40.0 28.0 41.0 66-67 34.74415072427668 37.0 34.0 39.5 28.0 41.0 68-69 34.39379888280364 36.0 34.0 39.0 28.0 41.0 70-71 34.014745549951 36.0 34.0 39.0 27.0 40.5 72-73 33.5982594198576 35.0 33.5 38.5 26.0 40.0 74-75 33.157344861453886 35.0 33.0 37.0 26.0 39.5 76-77 32.42842530592892 35.0 32.0 36.0 26.0 39.0 78-79 32.495507616403756 35.0 33.0 36.0 26.0 39.0 80-81 32.31503468159467 35.0 33.0 36.0 26.0 37.5 82-83 32.021975206674774 35.0 33.0 35.5 25.0 37.0 84-85 31.71950597983016 35.0 32.0 35.0 25.0 37.0 86-87 31.291496372334695 35.0 32.0 35.0 23.5 36.0 88-89 30.784681594665077 34.0 31.0 35.0 20.0 36.0 90-91 30.54943561153555 34.0 31.0 35.0 19.5 36.0 92-93 30.242747127529285 34.0 31.0 35.0 17.5 35.0 94-95 30.061425042635037 34.0 31.0 35.0 15.5 35.0 96-97 29.778050357959504 34.0 31.0 35.0 4.5 35.0 98-99 29.275438722991893 34.0 31.0 35.0 2.0 35.0 100-101 25.82853805252112 30.5 24.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13035353873320332 1101 2 0.11945664704770564 1101 3 0.1064760367787656 1101 4 0.09205469277207357 1101 5 0.11191174427931116 1101 6 0.09764680983776941 1101 7 0.11043175301861652 1101 8 0.08995209450887387 1101 9 0.2277109823589072 1101 10-11 0.16641399673024182 1101 12-13 0.19030194971922754 1101 14-15 0.3150703340677836 1101 16-17 0.39199419451381345 1101 18-19 0.36253977075712385 1101 20-21 0.3324229704362196 1101 22-23 0.2935601722846215 1101 24-25 0.2956159052318128 1101 26-27 0.3389399401545248 1101 28-29 0.22446446231722206 1101 30-31 0.19345555968720873 1101 32-33 0.266186708042639 1101 34-35 0.2416673633462736 1101 36-37 0.33761667924081706 1101 38-39 0.3334244107721105 1101 40-41 0.3655644938230438 1101 42-43 0.30952539906887466 1101 44-45 0.4976177879563224 1101 46-47 0.35599754294620567 1101 48-49 0.24929354383230162 1101 50-51 0.27532552457022064 1101 52-53 0.17527578564258306 1101 54-55 0.19717579991975498 1101 56-57 0.28071384637604524 1101 58-59 -0.017700285029469853 1101 60-61 0.28802108227025514 1101 62-63 0.22374841448117166 1101 64-65 0.3557241721326534 1101 66-67 0.13301331548467488 1101 68-69 0.2567626957071454 1101 70-71 0.33956602071782527 1101 72-73 0.3168833220239975 1101 74-75 0.19549491915138617 1101 76-77 0.15738761280199753 1101 78-79 0.05243575357969377 1101 80-81 0.17730253974905708 1101 82-83 0.2067160640543122 1101 84-85 0.2510122075951422 1101 86-87 0.15469915260320732 1101 88-89 0.1175285036195639 1101 90-91 0.23869559537045504 1101 92-93 0.4294662342176494 1101 94-95 0.3293719266056314 1101 96-97 0.10743203834588755 1101 98-99 0.2919993419219651 1101 100-101 0.3756022451316312 1102 1 0.049037585908870085 1102 2 0.09835174326124019 1102 3 0.13440899767261527 1102 4 0.11067666670130905 1102 5 0.007007957811275389 1102 6 0.07219677880114261 1102 7 -0.0031622879497277268 1102 8 0.13154116961749907 1102 9 0.07190713381762492 1102 10-11 0.05933764663713248 1102 12-13 0.16733484853983072 1102 14-15 0.19154457367051947 1102 16-17 0.22253423175776277 1102 18-19 0.27563722575401073 1102 20-21 0.19958622307433416 1102 22-23 0.2749381983553789 1102 24-25 0.18264259672777428 1102 26-27 0.2048617278640279 1102 28-29 0.22136079999568636 1102 30-31 0.20059398302674936 1102 32-33 0.2596690171674041 1102 34-35 0.11845196918115874 1102 36-37 0.1498451087876873 1102 38-39 0.19003521151699942 1102 40-41 0.14272153913651664 1102 42-43 0.1729642569211407 1102 44-45 0.17142287796253441 1102 46-47 0.22998885269171154 1102 48-49 0.27908870211908265 1102 50-51 0.14435097460125235 1102 52-53 0.14889465590950124 1102 54-55 0.11492874839895961 1102 56-57 0.2546430828751127 1102 58-59 0.19924571124613522 1102 60-61 0.17690997115914087 1102 62-63 0.3177893828238112 1102 64-65 0.43766085742129235 1102 66-67 0.45889785924630644 1102 68-69 0.2061729998660624 1102 70-71 0.3249788078065876 1102 72-73 0.04251957279991103 1102 74-75 0.18773576334753983 1102 76-77 0.17973398151708153 1102 78-79 0.16044320236925813 1102 80-81 0.09753841808549168 1102 82-83 0.09963971396120286 1102 84-85 0.20042251175405212 1102 86-87 0.05600269077825004 1102 88-89 0.10790715042279331 1102 90-91 0.21883215651260457 1102 92-93 0.17000006413695345 1102 94-95 0.16425709109973496 1102 96-97 0.20271447161714562 1102 98-99 0.23116756041979514 1102 100-101 0.47460907318874135 1103 1 0.1308244260755984 1103 2 0.09650517856551488 1103 3 0.12153528088828836 1103 4 0.043620021432374756 1103 5 0.15937795096338903 1103 6 0.14976774743356458 1103 7 0.14091284355556866 1103 8 0.17243211134643133 1103 9 0.16756475182161523 1103 10-11 0.18410639710420185 1103 12-13 0.17146988759107273 1103 14-15 0.2075291971970472 1103 16-17 0.2522016594698826 1103 18-19 0.22953316079519936 1103 20-21 0.27443748367978316 1103 22-23 0.31009403728217677 1103 24-25 0.2152621258556877 1103 26-27 0.19536124210655714 1103 28-29 0.18761266638721708 1103 30-31 0.2118033894875495 1103 32-33 0.23717795068192515 1103 34-35 0.1613862188193167 1103 36-37 0.20602120616489827 1103 38-39 0.18122942757154448 1103 40-41 0.09715356130597286 1103 42-43 0.21335819326479566 1103 44-45 0.3871794547526264 1103 46-47 0.36373134327777024 1103 48-49 0.1718047421814859 1103 50-51 0.1195664770513929 1103 52-53 0.05237132963757318 1103 54-55 0.16950745102234777 1103 56-57 0.2354596663453279 1103 58-59 0.09795479361444137 1103 60-61 0.03103841397473417 1103 62-63 0.13891415157427645 1103 64-65 0.13005188989612293 1103 66-67 -0.015720550427964497 1103 68-69 0.02934279230959902 1103 70-71 0.06428756806506897 1103 72-73 0.041231400422354625 1103 74-75 0.055018548926412336 1103 76-77 0.16638518325208196 1103 78-79 0.148896739705485 1103 80-81 0.17977822518563613 1103 82-83 0.39516098434280167 1103 84-85 0.3015128733192789 1103 86-87 0.2715266835368837 1103 88-89 0.4340530652558563 1103 90-91 0.5846002862472126 1103 92-93 0.5640814868496626 1103 94-95 0.6203402692499509 1103 96-97 0.49401033833200714 1103 98-99 0.604260943289713 1103 100-101 0.9603287949419794 1104 1 0.05918369937158019 1104 2 0.14110258708696932 1104 3 0.11898497454050982 1104 4 0.11081585445099051 1104 5 0.12727178594583677 1104 6 0.050202869387675264 1104 7 0.06369609861730652 1104 8 0.009971895088746408 1104 9 -0.025207751077473972 1104 10-11 -0.007076237116400819 1104 12-13 0.08704652785125688 1104 14-15 0.2085322140228385 1104 16-17 0.2483765702017564 1104 18-19 0.2702386492855027 1104 20-21 0.2743008601254431 1104 22-23 0.14831045935935094 1104 24-25 0.19906001629914272 1104 26-27 0.26414953557839027 1104 28-29 0.23411780624678613 1104 30-31 0.050962662191132324 1104 32-33 0.1354254296256059 1104 34-35 0.18206413823141787 1104 36-37 0.1425169860151101 1104 38-39 0.21123440377905922 1104 40-41 0.2255898843625488 1104 42-43 0.31576976561308356 1104 44-45 0.20846085040111006 1104 46-47 0.2531131013304204 1104 48-49 0.15916891467224303 1104 50-51 0.3308822026076257 1104 52-53 0.3194361299302173 1104 54-55 0.26574597131151023 1104 56-57 0.13747225348449632 1104 58-59 0.09144592159923803 1104 60-61 0.08683136375503153 1104 62-63 0.10765123898224971 1104 64-65 -0.23481353034903663 1104 66-67 -0.16997428221334587 1104 68-69 0.09302121674750907 1104 70-71 0.09828461788490017 1104 72-73 0.026097102060205657 1104 74-75 0.07168236464085709 1104 76-77 0.018009041205054643 1104 78-79 0.0632093343978184 1104 80-81 0.02490598487035811 1104 82-83 0.12214491385837789 1104 84-85 0.281871478677882 1104 86-87 0.24062513839483302 1104 88-89 0.253914538598341 1104 90-91 0.38422536751786396 1104 92-93 0.5721061768363782 1104 94-95 0.3688333373632702 1104 96-97 0.27946248454364664 1104 98-99 0.36737680450683996 1104 100-101 0.4305741101240059 1105 1 0.03665083471099706 1105 2 0.11798869369184217 1105 3 0.14092540376746854 1105 4 0.08074458753387859 1105 5 0.02348508266366167 1105 6 0.008045491873346577 1105 7 0.07383166367131366 1105 8 0.11371796200543116 1105 9 0.12166704507233561 1105 10-11 0.042897046255589544 1105 12-13 0.1812336027066479 1105 14-15 0.17595468144156712 1105 16-17 0.12719857187069294 1105 18-19 0.09758061392544448 1105 20-21 0.06352430987482904 1105 22-23 0.06059223049177831 1105 24-25 0.08254058516548923 1105 26-27 0.06742675151683386 1105 28-29 0.00685643706504635 1105 30-31 0.005109692403557631 1105 32-33 -0.05515466875004904 1105 34-35 0.003561951888016779 1105 36-37 -0.05098019742136728 1105 38-39 -0.10035562234585171 1105 40-41 -0.016574158941565997 1105 42-43 0.15523740783768858 1105 44-45 0.07340000813757541 1105 46-47 0.07314194602072632 1105 48-49 0.051267957614541615 1105 50-51 0.11631559835699079 1105 52-53 0.05933059657613882 1105 54-55 -0.01112509270894435 1105 56-57 -0.04201215658776647 1105 58-59 0.050773553721782605 1105 60-61 0.1929355874774643 1105 62-63 0.20521380561786629 1105 64-65 0.25193741043629103 1105 66-67 0.13147928770918327 1105 68-69 0.16495596044789096 1105 70-71 0.08893932320174258 1105 72-73 -0.009269190217210621 1105 74-75 0.07662538639664263 1105 76-77 0.09014037223494142 1105 78-79 0.10040871886382519 1105 80-81 0.04382542113702925 1105 82-83 0.14452057994722622 1105 84-85 0.012194477185385466 1105 86-87 0.17564456008231843 1105 88-89 0.30160541277567887 1105 90-91 0.3113684634884848 1105 92-93 0.4149182988780744 1105 94-95 0.2325519782978489 1105 96-97 0.3016943192862449 1105 98-99 0.21901610757178247 1105 100-101 0.47623138373043616 1106 1 0.006321659427463544 1106 2 -0.01741922756288261 1106 3 -0.01237567365572545 1106 4 0.03254478761387958 1106 5 -0.006972349032864145 1106 6 -0.01324125965516032 1106 7 0.026685992810548953 1106 8 -0.019786564752671154 1106 9 -0.11878981120352705 1106 10-11 0.057845851148471183 1106 12-13 0.16174989369757498 1106 14-15 0.17019024895539303 1106 16-17 -0.014333419785003798 1106 18-19 0.08004882886655196 1106 20-21 0.16970883410939308 1106 22-23 0.10168125222811852 1106 24-25 0.08745731208955476 1106 26-27 -0.07045877166187609 1106 28-29 0.017564980952663234 1106 30-31 0.0999852940821171 1106 32-33 0.12045557179659028 1106 34-35 0.20579805045272082 1106 36-37 0.2911179596379725 1106 38-39 0.2876697696508117 1106 40-41 0.3018469976817002 1106 42-43 0.2781061848885287 1106 44-45 0.3324949476941441 1106 46-47 0.3715174918904225 1106 48-49 0.2732947139821249 1106 50-51 0.22558087629556667 1106 52-53 0.2519462509299544 1106 54-55 0.28469780390320665 1106 56-57 0.24281951206976515 1106 58-59 0.2843035237791085 1106 60-61 0.29098848180874626 1106 62-63 0.2527820293636083 1106 64-65 0.23063020720949368 1106 66-67 0.4422788936500268 1106 68-69 0.39415796984457785 1106 70-71 0.323047660353879 1106 72-73 0.3638515933532105 1106 74-75 0.4452509560111011 1106 76-77 0.3198441415176063 1106 78-79 0.18334590164585762 1106 80-81 0.19628017955394483 1106 82-83 0.15992244195302874 1106 84-85 0.26971550257114174 1106 86-87 0.37515105020345274 1106 88-89 0.2813512986912521 1106 90-91 0.3622084390019076 1106 92-93 0.2782385593009913 1106 94-95 0.39232151192286935 1106 96-97 0.36871401461833386 1106 98-99 0.16553838293761203 1106 100-101 0.4662790327210615 1107 1 -0.0337501950803798 1107 2 -0.007329377280015592 1107 3 -0.08062316807089331 1107 4 0.02088039631615146 1107 5 -0.10842558466464425 1107 6 -0.029908605003299726 1107 7 0.06779980197817537 1107 8 -0.03119627189803964 1107 9 0.0012514383419386377 1107 10-11 -0.04120342326960014 1107 12-13 -0.04629187450341732 1107 14-15 0.06501444545011736 1107 16-17 -0.012851036570012297 1107 18-19 -0.09070046405450682 1107 20-21 -0.12294897540685668 1107 22-23 -0.15786779608185242 1107 24-25 -0.18495471471764802 1107 26-27 -0.09409528272474432 1107 28-29 -0.036955823492057505 1107 30-31 -0.2650665750423755 1107 32-33 -0.1734586914446652 1107 34-35 -0.06553165800714567 1107 36-37 0.02413770641120294 1107 38-39 0.0664708681146351 1107 40-41 0.009067739902050675 1107 42-43 -0.14379597353287465 1107 44-45 -0.09726009115858147 1107 46-47 0.08760735074610437 1107 48-49 -0.10743130373646181 1107 50-51 0.017886664448361955 1107 52-53 0.10807667350561445 1107 54-55 0.0893748836868653 1107 56-57 -0.11284869270343023 1107 58-59 0.01725308060010633 1107 60-61 0.009961308117013346 1107 62-63 0.037122418841043725 1107 64-65 0.03530139318036163 1107 66-67 -0.14858191020888256 1107 68-69 -0.004795467605632098 1107 70-71 0.2068779314049749 1107 72-73 0.1059165567437077 1107 74-75 0.1041995855388933 1107 76-77 0.11761420852133142 1107 78-79 0.038981065347392985 1107 80-81 0.16918024977961466 1107 82-83 0.2988460388505274 1107 84-85 0.08769545360605946 1107 86-87 0.24144169161459672 1107 88-89 0.4879826596333494 1107 90-91 0.06480539579418831 1107 92-93 0.25622570435951175 1107 94-95 0.17564191458816225 1107 96-97 0.041313250351556974 1107 98-99 0.1426053788005106 1107 100-101 0.368022386822048 1108 1 -0.07888810324644169 1108 2 -0.027849806146008405 1108 3 -0.012683518865316046 1108 4 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-1.0983325992428377 2316 94-95 -0.9391310963888131 2316 96-97 -0.7399558264711636 2316 98-99 -0.9693511750360599 2316 100-101 -0.8362259897185282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 21.0 5 36.0 6 81.0 7 173.0 8 369.0 9 654.0 10 1069.0 11 1739.0 12 2646.0 13 4017.0 14 5282.0 15 7042.0 16 9276.0 17 12993.0 18 12043.0 19 7455.0 20 6546.0 21 6771.0 22 7109.0 23 8220.0 24 9491.0 25 11638.0 26 14313.0 27 17796.0 28 22164.0 29 27627.0 30 34440.0 31 42747.0 32 53595.0 33 69102.0 34 96380.0 35 142203.0 36 235842.0 37 421050.0 38 461114.0 39 77535.0 40 65.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.723387196587915 18.662013097609943 13.204620856065393 23.409978849736746 2 16.034426804522493 19.750830183607437 39.6522899299466 24.56245308192347 3 19.85651029632033 24.23571029806834 28.780097167460372 27.127682238150957 4 12.703602100670034 15.501454671390661 36.317021130153485 35.47792209778582 5 13.795452335613392 37.814737246907384 33.57652511069815 14.813285306781074 6 33.91957191255147 35.24953377317615 16.59729255227913 14.233601761993256 7 30.145128462287246 32.03352463108812 19.666687424275697 18.15465948234893 8 27.44254239195391 35.10805428005511 18.751440741942496 18.697962586048483 9 27.261678066964045 15.864439702969541 19.56601302048339 37.307869209583025 10-11 26.006208185901436 26.294658037661538 26.42851777854557 21.270615997891458 12-13 26.40390593184995 23.27660398497475 27.49228894740479 22.827201135770515 14-15 22.92475803729159 24.648704779821855 25.78652219370782 26.640014989178727 16-17 22.958954859030406 28.40706789909338 26.044646607776613 22.589330634099603 18-19 22.637920655678244 26.497333739745464 28.593224060909378 22.271521543666907 20-21 23.360113402188563 25.79787807899096 28.624301990683648 22.217706528136826 22-23 22.906313459272035 25.873834602689477 28.488148351475772 22.731703586562716 24-25 22.755801398929158 26.058107810246234 28.703967984521046 22.48212280630357 26-27 22.71601471854283 26.431621923986242 28.505923689075154 22.346439668395774 28-29 22.365343640260495 26.229430734559756 28.828095062119885 22.577130563059868 30-31 22.42635561308254 26.24054394136254 28.833682892608238 22.499417552946692 32-33 22.3870381128667 26.29536795696123 28.705032178839122 22.612561751332944 34-35 22.926703628451307 26.237094944662477 28.390343321791768 22.445858105094448 36-37 22.916122688662497 26.18512550187092 28.2606997514544 22.638052058012182 38-39 22.59223368324567 26.350463688014468 28.53894984784085 22.518352780899015 40-41 22.810366955663405 26.083396357619606 28.617723767625975 22.48851291909102 42-43 22.680136982933945 26.221093383400895 28.33210331297017 22.766666320694988 44-45 22.66260640035486 26.471332130804015 28.162866972652473 22.703194496188654 46-47 22.669348055842605 26.655647184254544 27.656196519384206 23.01880824051864 48-49 22.998446501267903 26.70037361002565 27.511378073841865 22.789801814864582 50-51 22.885983620169743 26.74718553610293 27.338293971628108 23.028536872099224 52-53 23.195323099312574 26.718349372872368 27.333608845751378 22.752718682063676 54-55 22.77706643126681 26.552267945051035 27.456594509910175 23.214071113771983 56-57 22.850842056990402 27.195604298356855 27.169629300453156 22.783924344199587 58-59 23.085553997194953 26.598120043536984 27.404865271607687 22.91146068766038 60-61 23.157254750614747 26.672080099135187 27.658242524505887 22.51242262574418 62-63 23.046244675400157 26.693602395646582 27.52385213153332 22.73630079741994 64-65 22.783517431364057 26.50850496302653 27.567671799488373 23.140305806121038 66-67 22.760800238603256 27.061203263970146 27.230650903993876 22.94734559343273 68-69 22.698773225306365 26.75186026414604 27.610132772222386 22.93923373832521 70-71 22.6316335727747 26.83186299948106 27.586594925299757 22.949908502444487 72-73 22.84232902906684 26.786226893485317 27.332133197095747 23.039310880352097 74-75 22.69199968972837 26.902762619260656 27.232781290183727 23.172456400827247 76-77 22.837308129599656 26.97696118604947 27.19597973449063 22.98975094986024 78-79 22.762833304763085 26.93682366086868 27.249893442540035 23.050449591828198 80-81 22.54625992962001 27.484521755850704 27.07540119980291 22.89381711472637 82-83 22.961848607109808 27.24548166939247 26.809298753341704 22.98337097015602 84-85 22.816985270218968 26.950883431795898 26.83524113085825 23.396890167126887 86-87 22.741739438362476 26.81186824665674 27.254254529345896 23.192137785634888 88-89 22.821392447416898 26.91502588567011 27.075949429680147 23.18763223723284 90-91 22.816256395701167 27.147963841069906 27.113636089671868 22.92214367355706 92-93 22.713810734542204 26.875690624381964 27.222749064421386 23.187749576654447 94-95 22.68272217903679 26.84442919752847 27.06943653116152 23.403412092273218 96-97 22.738728157925284 26.838585446128004 27.024916523841064 23.39776987210565 98-99 22.588554570788894 26.61180694834532 27.036692990336274 23.762945490529518 100-101 22.45021480198586 26.852965862475926 27.090287301240906 23.606532034297302 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 157.0 1 121.0 2 192.5 3 329.0 4 275.0 5 241.0 6 517.5 7 1079.5 8 1693.5 9 1904.0 10 1618.0 11 1393.0 12 1460.5 13 1622.0 14 1796.5 15 1966.5 16 2118.0 17 2161.5 18 2241.5 19 2416.0 20 2624.0 21 2948.0 22 3458.5 23 4239.0 24 5078.0 25 6183.5 26 7939.0 27 9919.0 28 11818.5 29 13876.5 30 16549.5 31 19921.5 32 23788.0 33 27748.5 34 31950.5 35 35547.5 36 40038.0 37 45081.5 38 48611.5 39 50592.0 40 51360.0 41 54831.0 42 58997.0 43 62170.5 44 65148.5 45 67431.5 46 67054.5 47 66510.0 48 71837.0 49 73422.5 50 69993.0 51 68065.5 52 66393.0 53 66394.0 54 66216.5 55 61881.5 56 55180.0 57 49082.0 58 44451.0 59 40002.5 60 35130.5 61 29640.5 62 24656.5 63 19850.0 64 15801.0 65 12825.5 66 9090.0 67 7149.5 68 4968.0 69 2620.0 70 1997.5 71 1631.5 72 1383.5 73 1274.0 74 1037.0 75 886.0 76 599.5 77 269.5 78 125.0 79 60.5 80 39.5 81 28.0 82 15.0 83 6.0 84 2.5 85 2.0 86 3.0 87 1.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2552093404870609 2 0.018354096404891367 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 3.5506436497557704E-4 12-13 1.3656321729829888E-4 14-15 0.0 16-17 6.00878156112515E-4 18-19 0.0 20-21 8.193793037897931E-5 22-23 8.193793037897931E-5 24-25 0.0021576988333131216 26-27 0.0020757609029341427 28-29 0.0022123241202324415 30-31 8.466919472494529E-4 32-33 0.0013929448164426484 34-35 0.0013383195295233288 36-37 2.1850114767727816E-4 38-39 2.7312643459659775E-4 40-41 5.735655126528552E-4 42-43 0.003332142502078492 44-45 0.0037145195105137293 46-47 0.014175261955563422 48-49 0.014721514824756619 50-51 0.00600878156112515 52-53 0.006855473508374603 54-55 5.462528691931955E-4 56-57 0.0025947011286676785 58-59 0.0014475701033619679 60-61 0.0012290689556846896 62-63 0.0 64-65 9.286298776284323E-4 66-67 0.0 68-69 0.0 70-71 2.1850114767727816E-4 72-73 0.001556820677200607 74-75 0.0 76-77 0.18635416632525864 78-79 0.09988233713197579 80-81 0.001092505738386391 82-83 0.0 84-85 4.916275822738759E-4 86-87 0.11807255767610919 88-89 0.5754773976950314 90-91 0.30590160674818945 92-93 0.29022414940234476 94-95 0.0025673884852080186 96-97 0.002266949407151761 98-99 0.002485450554829039 100-101 7.920666603301334E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1830654.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.75799376706213 #Duplication Level Percentage of deduplicated Percentage of total 1 71.28926717986045 34.04632377634202 2 14.901332740884515 14.233155123201632 3 5.620366641791391 8.052523051418314 4 2.73537099739775 5.22543324177297 5 1.521955692379678 3.6342775235206695 6 0.9453208197039493 2.708797548917717 7 0.584702920873206 1.9546966915451915 8 0.42323693164753545 1.6170357394890797 9 0.30049063345038823 1.2915746819445775 >10 1.4581455859290797 12.58825832600996 >50 0.1280747416953825 4.2507496611156155 >100 0.08568849916184676 7.364827463530431 >500 0.004677570268400621 1.5699509719108193 >1k 0.0012549578768879714 1.1186459153293895 >5k 1.1408707971708831E-4 0.3437502839517373 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6309 0.344630935173987 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4585 0.2504569405250801 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3289 0.179662568677642 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 5.462528691931954E-5 0.0 0.0 0.0 5 0.0 1.0925057383863908E-4 0.0 0.0 0.0 6 0.0 1.0925057383863908E-4 0.0 0.0 0.0 7 0.0 1.6387586075795863E-4 0.0 0.0 0.0 8 0.0 1.6387586075795863E-4 0.0 0.0 0.0 9 0.0 1.6387586075795863E-4 0.0 0.0 0.0 10-11 0.0 1.6387586075795863E-4 0.0 0.0 0.0 12-13 0.0 2.1850114767727816E-4 0.0 0.0 0.0 14-15 0.0 2.1850114767727816E-4 0.0 0.0 0.0 16-17 0.0 2.1850114767727816E-4 0.0 2.731264345965977E-5 0.0 18-19 0.0 2.1850114767727816E-4 0.0 8.193793037897931E-5 0.0 20-21 0.0 2.1850114767727816E-4 0.0 2.1850114767727816E-4 0.0 22-23 0.0 2.1850114767727816E-4 0.0 4.096896518948966E-4 0.0 24-25 0.0 2.1850114767727816E-4 0.0 6.555034430318345E-4 0.0 26-27 0.0 2.1850114767727816E-4 0.0 7.374413734108139E-4 0.0 28-29 0.0 2.1850114767727816E-4 0.0 0.0010925057383863908 0.0 30-31 0.0 2.731264345965977E-4 0.0 0.0028951402067239358 0.0 32-33 0.0 2.731264345965977E-4 0.0 0.006008781561125149 0.0 34-35 0.0 2.731264345965977E-4 0.0 0.010324179227751393 0.0 36-37 0.0 2.731264345965977E-4 0.0 0.019009599847923202 0.0 38-39 0.0 2.731264345965977E-4 0.0 0.032010418134721254 0.0 40-41 0.0 2.731264345965977E-4 0.0 0.046786558246397186 0.0 42-43 0.0 2.731264345965977E-4 0.0 0.06057944319352537 0.0 44-45 0.0 2.731264345965977E-4 0.0 0.0781414729380866 0.0 46-47 0.0 2.731264345965977E-4 0.0 0.09947264748008089 0.0 48-49 0.0 2.731264345965977E-4 0.0 0.12419058981107298 0.0 50-51 0.0 2.731264345965977E-4 0.0 0.1535243688867476 0.0 52-53 0.0 2.731264345965977E-4 0.0 0.18318589968393809 0.0 54-55 0.0 4.370022953545563E-4 0.0 0.21232849025539507 0.0 56-57 0.0 4.370022953545563E-4 0.0 0.25007456351664487 0.0 58-59 0.0 4.370022953545563E-4 0.0 0.2962329309634699 0.0 60-61 0.0 4.370022953545563E-4 0.0 0.3474987627372513 0.0 62-63 0.0 4.370022953545563E-4 0.0 0.39980247496249977 0.0 64-65 0.0 4.916275822738759E-4 0.0 0.4531167549957556 0.0 66-67 0.0 4.916275822738759E-4 0.0 0.5130406947462491 0.0 68-69 0.0 4.916275822738759E-4 0.0 0.5813769286823178 0.0 70-71 0.0 4.916275822738759E-4 0.0 0.6637791740001114 0.0 72-73 0.0 4.916275822738759E-4 0.0 0.7520809503051915 0.0 74-75 0.0 5.462528691931954E-4 0.0 0.8375422116904669 0.0 76-77 0.0 5.462528691931954E-4 0.0 0.9316615810524544 0.0 78-79 0.0 5.462528691931954E-4 0.0 1.0453368031315584 0.0 80-81 0.0 5.462528691931954E-4 0.0 1.1779669997716664 0.0 82-83 0.0 5.462528691931954E-4 0.0 1.3117170147936204 0.0 84-85 0.0 5.462528691931954E-4 0.0 1.4468599746320168 0.0 86-87 0.0 5.462528691931954E-4 0.0 1.5921632378374069 0.0 88-89 0.0 5.462528691931954E-4 0.0 1.761447002000378 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6920 0.0 36.226276 1 GGTATCA 4350 0.0 34.883545 1 GTACATG 8705 0.0 30.81978 1 TACATGG 8715 0.0 30.379538 2 ATCAACG 8530 0.0 29.196428 3 TCAACGC 8555 0.0 29.111115 4 CAACGCA 8780 0.0 28.419125 5 ACATGGG 8815 0.0 28.19866 3 TATCAAC 9000 0.0 27.783216 2 AACGCAG 9015 0.0 27.625683 6 GTACCTG 1645 0.0 26.025305 1 TACCTGG 1615 0.0 24.97243 2 CATGGGG 3900 0.0 24.57006 4 ACGCAGA 10560 0.0 23.538939 7 CGCAGAG 10730 0.0 22.94495 8 CATGGGA 5095 0.0 22.066004 4 GTATAGG 650 0.0 21.95468 1 AGTACAT 5920 0.0 21.800255 2 GCAGAGT 11520 0.0 21.1244 9 TATCACG 115 0.0037479918 20.629398 2 >>END_MODULE