FastQCFastQC Report
Thu 26 May 2016
SRR936283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512628
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44950.2971649341411107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32460.21459340961558293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22140.14636777846238466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22900.052.7581021
GTATCAA42650.045.212641
ATCAACG53200.035.791843
TCAACGC53700.035.4585844
TATCAAC54450.035.0560042
CAACGCA54350.035.0345155
GTAGGAC2500.034.1037453
AACGCAG56250.033.9353336
ACGCAGA67300.028.3634857
GTACATG63750.027.9442311
TACATGG62650.027.5947862
CGCAGAG69500.027.53388
TAACGCA1057.041047E-527.0664654
TACCTGG12650.026.9585932
ACATGGG63600.025.9174183
GTACCTG14800.024.3296781
GTGGTAT11300.023.8990651
GCAGAGT79400.023.6831599
CATGGGA28300.022.9299834
ATAACGC1553.9933162E-521.391243
Produced by FastQC (version 0.11.5)