##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936283_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1512628 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.43471296313436 34.0 31.0 34.0 31.0 34.0 2 32.548200218427795 34.0 31.0 34.0 31.0 34.0 3 32.61923883466391 34.0 31.0 34.0 31.0 34.0 4 36.08485761205002 37.0 37.0 37.0 35.0 37.0 5 36.0931888078232 37.0 37.0 37.0 35.0 37.0 6 36.18377089409954 37.0 37.0 37.0 35.0 37.0 7 36.14577410969518 37.0 37.0 37.0 35.0 37.0 8 36.15323000764233 37.0 37.0 37.0 35.0 37.0 9 37.92168199980431 39.0 38.0 39.0 35.0 39.0 10-11 37.87209413021576 39.0 38.0 39.0 35.0 39.0 12-13 37.80525020031362 39.0 38.0 39.0 35.0 39.0 14-15 39.188640564633204 41.0 39.0 41.0 36.0 41.0 16-17 39.15217356812118 40.5 39.0 41.0 36.0 41.0 18-19 39.11367335524663 40.0 39.0 41.0 36.0 41.0 20-21 39.04392421666133 40.0 39.0 41.0 36.0 41.0 22-23 38.90076079511949 40.0 39.0 41.0 36.0 41.0 24-25 39.060230935828244 40.5 39.0 41.0 36.0 41.0 26-27 39.030447671205344 40.5 39.0 41.0 36.0 41.0 28-29 38.92423682491663 40.0 39.0 41.0 35.5 41.0 30-31 38.78570177201533 40.0 38.5 41.0 35.0 41.0 32-33 38.62352805845191 40.0 38.0 41.0 35.0 41.0 34-35 38.38945960275758 40.0 38.0 41.0 35.0 41.0 36-37 38.214516391340105 40.0 38.0 41.0 34.5 41.0 38-39 38.06300987420569 40.0 38.0 41.0 34.0 41.0 40-41 37.88862925980479 40.0 38.0 41.0 33.5 41.0 42-43 37.705674825535425 40.0 38.0 41.0 33.0 41.0 44-45 37.5150370745484 40.0 37.5 41.0 33.0 41.0 46-47 37.22771560489426 40.0 37.0 41.0 32.5 41.0 48-49 37.04907022744521 40.0 37.0 41.0 32.0 41.0 50-51 36.745699206943144 39.5 36.5 40.5 31.5 41.0 52-53 36.75694718066835 39.5 36.0 40.5 32.0 41.0 54-55 36.826473197640134 40.0 36.0 41.0 32.0 41.0 56-57 36.65394829396256 39.5 36.0 41.0 32.0 41.0 58-59 36.41609536515257 39.0 35.0 41.0 31.0 41.0 60-61 36.11916016363574 39.0 35.0 41.0 31.0 41.0 62-63 35.815493630952226 38.0 35.0 40.5 31.0 41.0 64-65 35.478764111202494 37.5 35.0 40.0 30.0 41.0 66-67 35.120185861956806 37.0 35.0 40.0 30.0 41.0 68-69 34.74064938636598 36.5 35.0 39.0 29.0 41.0 70-71 34.347503814553214 36.0 34.0 39.0 29.0 41.0 72-73 33.957272706838694 35.0 34.0 38.5 29.0 40.0 74-75 33.565043421118745 35.0 34.0 37.0 28.0 39.5 76-77 33.18602789317664 35.0 34.0 37.0 27.5 39.0 78-79 32.821484859463126 35.0 33.5 36.5 27.0 39.0 80-81 32.46308081035126 35.0 33.0 36.0 26.0 37.5 82-83 32.14056099715198 35.0 33.0 35.5 26.0 37.0 84-85 31.853681804118395 35.0 33.0 35.0 25.0 37.0 86-87 31.579716890074756 35.0 33.0 35.0 25.0 36.0 88-89 31.301582742088605 35.0 32.5 35.0 24.0 36.0 90-91 31.00221733301248 35.0 32.0 35.0 22.5 36.0 92-93 30.734344465394003 34.0 32.0 35.0 20.0 35.0 94-95 30.41263912872167 34.0 31.5 35.0 18.5 35.0 96-97 30.062760639099633 34.0 31.0 35.0 10.0 35.0 98-99 29.641131857932024 34.0 31.0 35.0 2.0 35.0 100-101 26.36139520093506 31.5 25.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12880554186951088 1101 2 0.09195890380903649 1101 3 0.1348693285803222 1101 4 0.07229835915854466 1101 5 0.21735158586790249 1101 6 0.17163081261809054 1101 7 0.2116943370247668 1101 8 0.13682425654395303 1101 9 0.2281136572110114 1101 10-11 0.2716219517252938 1101 12-13 0.18799131135160962 1101 14-15 0.2806730977201255 1101 16-17 0.24660231106615527 1101 18-19 0.27048747965038444 1101 20-21 0.3470142582538642 1101 22-23 0.265792653189159 1101 24-25 0.2286579691299977 1101 26-27 0.1766156887361845 1101 28-29 0.16525980237309312 1101 30-31 0.24883959042684012 1101 32-33 0.24650141390367963 1101 34-35 0.14011625141854722 1101 36-37 0.023266787267807842 1101 38-39 0.10267605604779106 1101 40-41 0.0049266279993531725 1101 42-43 -0.007583394545662259 1101 44-45 -0.12104878392761265 1101 46-47 -0.25937650753757424 1101 48-49 -0.2635824778075957 1101 50-51 -0.017565595406985324 1101 52-53 -0.024764891389637 1101 54-55 -0.026207568549736493 1101 56-57 -0.036489423005200194 1101 58-59 -0.17380984447333248 1101 60-61 -0.19815134373561705 1101 62-63 -0.17594602884645383 1101 64-65 -0.13415248605965502 1101 66-67 0.0706786813723852 1101 68-69 0.02873351953299874 1101 70-71 0.05393761963547661 1101 72-73 0.04681332367147917 1101 74-75 0.06520765690850538 1101 76-77 0.015202236765055943 1101 78-79 -0.028809450117442736 1101 80-81 0.08035055006534719 1101 82-83 0.08383653056767315 1101 84-85 0.12707392797430117 1101 86-87 0.13466071341035857 1101 88-89 0.0654063363438695 1101 90-91 0.18597753112293347 1101 92-93 -0.03623220711929065 1101 94-95 0.15197171871421133 1101 96-97 0.25261875734324946 1101 98-99 0.2545804661028903 1101 100-101 0.5419486075370301 1102 1 0.3196706160112157 1102 2 0.27014001412707955 1102 3 0.31162600349696845 1102 4 0.27649781719331656 1102 5 0.2646453130993365 1102 6 0.2529626751678009 1102 7 0.25008457052712174 1102 8 0.23358544268768355 1102 9 0.24135843453869654 1102 10-11 0.3467593742078918 1102 12-13 0.39075320198833907 1102 14-15 0.37160290488061776 1102 16-17 0.3791396909083815 1102 18-19 0.37627266490423494 1102 20-21 0.360076732569226 1102 22-23 0.4931422763781015 1102 24-25 0.35228026818727187 1102 26-27 0.4538305930889024 1102 28-29 0.41673135846834697 1102 30-31 0.4348713173307459 1102 32-33 0.3079954643367344 1102 34-35 0.36726657724656064 1102 36-37 0.3875777282294379 1102 38-39 0.34338400971192584 1102 40-41 0.2956674889021542 1102 42-43 0.3555611161252443 1102 44-45 0.32522281048218105 1102 46-47 0.397156892537204 1102 48-49 0.24466132620739245 1102 50-51 0.06716668180482799 1102 52-53 0.18044804779840007 1102 54-55 0.26793447948556803 1102 56-57 0.20588783917433773 1102 58-59 0.23125045456540505 1102 60-61 0.14573625255281542 1102 62-63 0.3653556455603706 1102 64-65 0.09642529089415319 1102 66-67 0.15995540189568658 1102 68-69 0.22803165293554173 1102 70-71 0.35754011255192353 1102 72-73 0.26605509763428614 1102 74-75 0.2939940750250969 1102 76-77 0.13983801697560239 1102 78-79 0.2189905542083963 1102 80-81 0.10514066986004877 1102 82-83 0.1300117206726341 1102 84-85 0.20026704248222416 1102 86-87 0.23070736444222817 1102 88-89 0.14469655964960992 1102 90-91 0.22495020730025672 1102 92-93 0.081071284137888 1102 94-95 0.04341490969670403 1102 96-97 0.195697731626165 1102 98-99 0.2964939370595481 1102 100-101 0.42994812551550865 1103 1 0.17364178547849463 1103 2 0.12747555400244437 1103 3 0.20430258747892793 1103 4 0.14179873133215892 1103 5 0.07423710282315454 1103 6 0.10814623036772275 1103 7 0.12836710489825265 1103 8 0.10632737426063699 1103 9 0.14234289175360004 1103 10-11 0.1943806734004383 1103 12-13 0.17908126144374137 1103 14-15 0.22089881186638394 1103 16-17 0.28322785559885943 1103 18-19 0.10252354388389051 1103 20-21 0.20985607836588116 1103 22-23 0.1178437263914347 1103 24-25 0.2964347503773297 1103 26-27 0.20390333623285528 1103 28-29 0.21870027289816107 1103 30-31 0.2768883634741499 1103 32-33 0.3516610519800949 1103 34-35 0.22992449048824426 1103 36-37 0.2650888821781763 1103 38-39 0.20651020694283773 1103 40-41 0.36253056205751477 1103 42-43 0.2449081857889297 1103 44-45 0.15025281702906312 1103 46-47 0.09796888788164182 1103 48-49 0.24494472137239853 1103 50-51 0.3029620674647333 1103 52-53 0.24688981691166845 1103 54-55 0.2648459268316472 1103 56-57 0.3350008577723358 1103 58-59 0.3052317474535542 1103 60-61 0.41573607069495466 1103 62-63 0.34716076667770324 1103 64-65 0.346104008675006 1103 66-67 0.33369179286583517 1103 68-69 0.4608354768001419 1103 70-71 0.3663026304346104 1103 72-73 0.46383309768277314 1103 74-75 0.42998889813802066 1103 76-77 0.36811358012877093 1103 78-79 0.3806349129775839 1103 80-81 0.35010320714091847 1103 82-83 0.5482847984350627 1103 84-85 0.5119411047018616 1103 86-87 0.6246463450683031 1103 88-89 0.3955922096096032 1103 90-91 0.5058584660718672 1103 92-93 0.5834505911382024 1103 94-95 0.5521925824010516 1103 96-97 0.8951139678958953 1103 98-99 0.6661518624250817 1103 100-101 1.0894556544309069 1104 1 0.2551341996218355 1104 2 0.12288912602046764 1104 3 0.18556409322386713 1104 4 0.21348745728911922 1104 5 0.1905165416602017 1104 6 0.17034866799865256 1104 7 0.18082933431487191 1104 8 0.2087285042041387 1104 9 0.2493423521738407 1104 10-11 0.20654502304812894 1104 12-13 0.2914666007441866 1104 14-15 0.3944133805199712 1104 16-17 0.40535340311362233 1104 18-19 0.21778531941870938 1104 20-21 0.3817479402334598 1104 22-23 0.3493770057667973 1104 24-25 0.3948049020944637 1104 26-27 0.3327974357694572 1104 28-29 0.4499042499184114 1104 30-31 0.4390340658800369 1104 32-33 0.39310752249364356 1104 34-35 0.357654129287198 1104 36-37 0.4091308900496955 1104 38-39 0.1618084041116319 1104 40-41 0.19960127874639966 1104 42-43 0.31257585226515516 1104 44-45 0.28235860640251786 1104 46-47 0.31035502068174026 1104 48-49 0.26898379329521305 1104 50-51 0.3444779691977331 1104 52-53 0.40310410942738173 1104 54-55 0.4844330689633054 1104 56-57 0.46456546451391034 1104 58-59 0.39349668111697866 1104 60-61 0.3987135194348781 1104 62-63 0.43894090379443895 1104 64-65 0.5082219385931097 1104 66-67 0.42069506208440544 1104 68-69 0.3691406408958713 1104 70-71 0.37110131957319226 1104 72-73 0.3027824247950761 1104 74-75 0.2685097354388475 1104 76-77 0.4201592222286905 1104 78-79 0.3307297361858659 1104 80-81 0.45300820936271435 1104 82-83 0.46896855708420304 1104 84-85 0.1293631830361477 1104 86-87 0.22018370303186785 1104 88-89 0.35419930032248104 1104 90-91 0.37530595226497 1104 92-93 0.2430015496857827 1104 94-95 -0.043969656537562685 1104 96-97 0.14235457286002884 1104 98-99 0.16592769877371438 1104 100-101 0.43562186086777643 1105 1 0.24052490620715616 1105 2 0.2530026240849992 1105 3 0.22279889798835484 1105 4 0.2028811200848395 1105 5 0.19467113615293385 1105 6 0.20000891972801327 1105 7 0.2280869878768499 1105 8 0.20549649861364117 1105 9 0.19252707017570714 1105 10-11 0.2707110811987121 1105 12-13 0.26962553533067535 1105 14-15 0.35583689593702417 1105 16-17 0.28411025382695954 1105 18-19 0.2912020488999403 1105 20-21 0.39107639366038427 1105 22-23 0.3246528500337931 1105 24-25 0.34292203555185097 1105 26-27 0.31559777771622777 1105 28-29 0.3414306143685053 1105 30-31 0.4200515538471308 1105 32-33 0.3750971639795253 1105 34-35 0.1627778347182982 1105 36-37 0.3843880862431277 1105 38-39 0.3902688976770605 1105 40-41 0.4465479020710177 1105 42-43 0.3648751106607193 1105 44-45 0.2840123960836536 1105 46-47 0.24730220344856235 1105 48-49 0.2625875040655501 1105 50-51 0.2734616884480445 1105 52-53 0.2660405234224612 1105 54-55 0.2917398258473938 1105 56-57 0.3283621435864532 1105 58-59 0.3108955300401064 1105 60-61 0.26902033913352597 1105 62-63 0.010719792604810152 1105 64-65 0.08827198413366943 1105 66-67 0.07075414703324157 1105 68-69 0.1083196054763107 1105 70-71 0.18172890576655476 1105 72-73 0.3032773348524742 1105 74-75 0.17708927241699968 1105 76-77 0.3137979717477677 1105 78-79 0.2265927592152508 1105 80-81 0.3129832602032536 1105 82-83 0.30110669612424346 1105 84-85 0.25636245281684467 1105 86-87 0.34566119186265 1105 88-89 0.29621655077199804 1105 90-91 0.4815221894370616 1105 92-93 0.3236242873800137 1105 94-95 0.2789985377007689 1105 96-97 0.3892229233495925 1105 98-99 0.4150347995675361 1105 100-101 0.6501308963501558 1106 1 0.1499049368514278 1106 2 0.1016157049455444 1106 3 0.1426682934225596 1106 4 0.16832752398094186 1106 5 0.11562012191757276 1106 6 0.09510473456725776 1106 7 0.06439925291387993 1106 8 0.08527065636315001 1106 9 0.10711250796779126 1106 10-11 0.09626959197712637 1106 12-13 0.12746877823992975 1106 14-15 0.13840601438494105 1106 16-17 0.14387654694770902 1106 18-19 0.21661787557923162 1106 20-21 0.12420516043199825 1106 22-23 0.14725134828244535 1106 24-25 0.17018399043417531 1106 26-27 0.1562631291410881 1106 28-29 0.16708803171446363 1106 30-31 0.21194710659593596 1106 32-33 0.13172019201486762 1106 34-35 0.1415198677104712 1106 36-37 0.12912077268440925 1106 38-39 0.03921404596267308 1106 40-41 0.04634774200043523 1106 42-43 0.02964315440375742 1106 44-45 -0.021388573057016913 1106 46-47 0.048776605806232 1106 48-49 0.15399719326902783 1106 50-51 0.2371294505109347 1106 52-53 0.13052703069867988 1106 54-55 0.24302103709601397 1106 56-57 0.24075822583264994 1106 58-59 0.3876630718263314 1106 60-61 0.4069693761475719 1106 62-63 0.21418281567806474 1106 64-65 0.12902939290613347 1106 66-67 -0.009961036058854233 1106 68-69 0.07155701620295218 1106 70-71 0.14289047599093152 1106 72-73 0.07765636685173405 1106 74-75 0.10496387325834888 1106 76-77 0.0013691281978438496 1106 78-79 0.08528760443485339 1106 80-81 0.13438003168214152 1106 82-83 0.153991439079725 1106 84-85 0.20076810098055375 1106 86-87 0.23751847519380576 1106 88-89 0.11165881160849622 1106 90-91 0.1151806451822388 1106 92-93 0.07429400218903481 1106 94-95 0.10499362637900944 1106 96-97 0.11860066453433404 1106 98-99 0.15178191822523956 1106 100-101 0.2507775489256261 1107 1 -0.11860569326906045 1107 2 0.008341552938297525 1107 3 0.1402381010914624 1107 4 -0.04584672553949787 1107 5 0.049385555141732596 1107 6 0.09777538388488693 1107 7 0.09613711143746428 1107 8 0.06953008580011044 1107 9 0.10422456043077233 1107 10-11 0.12041851960302807 1107 12-13 0.1279563441993048 1107 14-15 0.19586119108387123 1107 16-17 -0.04083482336639577 1107 18-19 0.09234029361074647 1107 20-21 0.1267908121468011 1107 22-23 0.22742453644151794 1107 24-25 0.3019184490536446 1107 26-27 0.2578995670467634 1107 28-29 0.27186691722589984 1107 30-31 0.2904678920821908 1107 32-33 0.44902856183968254 1107 34-35 0.2575866812464227 1107 36-37 0.2252887851053913 1107 38-39 0.2782107983151363 1107 40-41 0.3505142505797778 1107 42-43 0.11038545694693624 1107 44-45 0.40101970221104466 1107 46-47 0.4308277728849035 1107 48-49 0.3316367630284063 1107 50-51 0.40230911080631415 1107 52-53 0.47417874452136033 1107 54-55 0.5955610364751607 1107 56-57 0.5366543444725522 1107 58-59 0.5803277540658698 1107 60-61 0.4299347429504152 1107 62-63 0.5244277615235049 1107 64-65 0.41782414660771394 1107 66-67 0.39930858077194387 1107 68-69 0.4866564831078435 1107 70-71 0.5350361554765541 1107 72-73 0.5105085069928279 1107 74-75 0.46868931079891496 1107 76-77 0.45237626356182403 1107 78-79 0.4244095619518262 1107 80-81 0.4020066867332872 1107 82-83 0.43974401770989857 1107 84-85 0.34279786344552576 1107 86-87 0.19189742850622338 1107 88-89 0.33700913723554393 1107 90-91 0.4431041069350421 1107 92-93 0.3334043901772219 1107 94-95 0.2647982353678664 1107 96-97 0.24106422519971105 1107 98-99 0.22959365124506093 1107 100-101 0.20169352744020586 1108 1 0.057277568022477965 1108 2 0.04259435927651367 1108 3 0.10143189740361436 1108 4 0.03643309301155284 1108 5 0.07481397759587338 1108 6 -0.01698310954882487 1108 7 0.014835712788361377 1108 8 2.8457586969921067E-4 1108 9 8.712659321048477E-4 1108 10-11 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-0.5996309342068784 2316 76-77 -0.9667503351362612 2316 78-79 -0.9768731078928852 2316 80-81 -0.8335234145872725 2316 82-83 -0.8498420447447685 2316 84-85 -0.857324107945729 2316 86-87 -0.9800847532238173 2316 88-89 -0.9327105255882451 2316 90-91 -1.0188178634481808 2316 92-93 -0.9780386981640632 2316 94-95 -0.777759562439126 2316 96-97 -1.0431185779337895 2316 98-99 -0.841085150246272 2316 100-101 -0.9501058713168966 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5315.0 3 1085.0 4 958.0 5 795.0 6 841.0 7 858.0 8 986.0 9 1094.0 10 1298.0 11 1754.0 12 2690.0 13 5200.0 14 6190.0 15 4751.0 16 6089.0 17 8718.0 18 8276.0 19 4794.0 20 4455.0 21 4323.0 22 4520.0 23 4765.0 24 5281.0 25 6350.0 26 7370.0 27 8923.0 28 10916.0 29 13843.0 30 17905.0 31 23772.0 32 32378.0 33 46693.0 34 72993.0 35 122779.0 36 225552.0 37 388936.0 38 376051.0 39 73064.0 40 67.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.16719552505123 17.80753699253073 12.824209589289328 24.201057893128723 2 15.729889871729688 18.94448231382425 40.222901152984875 25.10272666146119 3 20.080844891261375 23.21090678771803 28.165135673700814 28.54311264731978 4 12.767985825543112 14.753335360774306 35.43746446468947 37.041214348993115 5 14.247840910668607 37.11253577546397 33.37595426029662 15.263669053570803 6 34.6419440806415 34.27753143488801 16.48868053136018 14.5918439531103 7 30.663221759507486 31.434094663986023 19.35717397728768 18.54550959921881 8 27.610778846929424 35.018095652415376 18.566787304349436 18.804338196305764 9 27.13741005624563 15.803257314345446 19.118159464664824 37.94117316474409 10-11 26.30963242099696 25.73816607166468 26.10230728546873 21.84989422186963 12-13 26.463584031332786 22.648140177727882 27.26663876652274 23.621637024416593 14-15 23.21834642449478 24.218588071875384 25.051990549637047 27.511074953992786 16-17 23.319990452368625 27.51202214733106 25.671557513569336 23.496429886730972 18-19 23.201428087371177 26.116081685275326 27.73977074569632 22.942719481657175 20-21 23.816523793483817 25.35021917725806 27.99231251592882 22.840944513329305 22-23 23.42490564590695 25.156518539748717 27.8716552932671 23.546920521077237 24-25 23.2258403653055 25.421323240618417 28.02354966813852 23.32928672593756 26-27 23.02383676399954 25.7700776531949 27.892904638068543 23.313180944737013 28-29 22.87448014122968 25.851080711173406 28.026916948222397 23.247522199374515 30-31 23.096086668052905 25.6331060801655 27.858122088604603 23.412685163176985 32-33 22.893498377503498 25.628866156600665 27.91893996895174 23.5586954969441 34-35 23.244606683171025 25.86625508472976 27.794998802423777 23.094139429675444 36-37 23.401940958905108 25.505962711236734 27.7510467679112 23.34104956194696 38-39 23.047939807709856 25.820070180095726 27.789725169435986 23.342264842758425 40-41 23.15763202489391 25.780241385467356 27.87668273203797 23.185443857600763 42-43 23.202432077350732 25.6244543107748 27.647424446412693 23.525689165461767 44-45 23.034863915097784 25.918561473521823 27.640648502541676 23.405926108838713 46-47 23.325560978686045 26.112555865527753 27.32925851736665 23.23262463841955 48-49 23.392859336002758 26.147758455809257 27.099085749907143 23.360296458280846 50-51 23.299841220362435 26.344506334089225 27.035860030919373 23.319792414628974 52-53 23.49281334206978 26.15095732484119 26.99847908795731 23.35775024513172 54-55 23.42724383707133 26.240476605348533 26.8508036077299 23.481475949850232 56-57 23.180689783347493 26.531004407391674 26.882301401869157 23.406004407391674 58-59 23.279773050247947 26.180896050614997 27.063518982283824 23.475811916853235 60-61 23.186570926129406 26.365230215681702 27.07087253337971 23.377326324809186 62-63 23.11241687300116 26.363923272440182 27.248299296023543 23.275360558535112 64-65 23.08761805000999 26.41575376429096 27.040032287636105 23.45659589806294 66-67 23.10178729877044 26.585164975554136 26.782437471753035 23.530610253922386 68-69 23.17820524224437 26.399909987294667 27.04171518015153 23.38016959030943 70-71 23.18470394862379 26.244126610028147 27.018373725973397 23.552795715374668 72-73 23.213575139679175 26.4578489988069 26.76307723963752 23.565498621876404 74-75 23.010762324396538 26.821272393849625 26.725847004057652 23.442118277696185 76-77 23.282057409122135 26.581870558990545 26.631420691577805 23.504651340309515 78-79 23.162877851319045 26.66814464593712 26.702062942667794 23.46691456007604 80-81 23.212524928951037 26.775875579103392 26.588391945339346 23.42320754660623 82-83 23.390604874160033 26.75374941131529 26.613128855490725 23.242516859033948 84-85 23.204508637561602 26.80665940716102 26.446361358100617 23.54247059717676 86-87 23.153747863460612 26.693815237052153 26.70546456248652 23.446972337000712 88-89 23.21380264009116 26.732043260876708 26.48279831473373 23.571355784298397 90-91 23.080240527694816 26.877263413150672 26.58334340401402 23.459152655140496 92-93 23.15449297397066 26.74419145058542 26.604202007057566 23.497113568386354 94-95 23.190659231612212 26.558006102656588 26.672273269448155 23.57906139628305 96-97 23.25854786996308 26.522975319370328 26.628655119199184 23.58982169146741 98-99 23.071076153661636 26.58611574953128 26.52559971095629 23.817208385850794 100-101 23.07244112829024 26.62805316479301 26.47910494362763 23.820400763289122 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 137.0 1 99.5 2 151.0 3 281.5 4 238.5 5 212.5 6 449.5 7 903.5 8 1477.0 9 1683.5 10 1440.5 11 1285.5 12 1408.0 13 1547.0 14 1642.0 15 1763.0 16 1856.0 17 1970.5 18 2053.5 19 2137.5 20 2284.5 21 2456.0 22 2812.0 23 3346.0 24 4013.0 25 5053.0 26 6555.0 27 8252.0 28 9810.0 29 11623.0 30 13465.5 31 15695.0 32 18951.0 33 21364.5 34 23276.0 35 25861.5 36 29591.5 37 33078.0 38 34912.5 39 36479.5 40 38081.0 41 40264.5 42 41714.0 43 43719.5 44 47022.0 45 49956.0 46 52187.0 47 54212.5 48 56533.5 49 57290.0 50 56652.0 51 56278.5 52 56592.5 53 57263.5 54 58200.5 55 56329.5 56 52335.5 57 48536.5 58 45795.0 59 42497.0 60 36652.5 61 30840.5 62 25262.0 63 19419.5 64 14399.0 65 10843.0 66 8023.0 67 6252.5 68 4810.5 69 3500.5 70 2635.0 71 1792.0 72 1344.0 73 1092.5 74 808.5 75 601.0 76 416.0 77 236.5 78 171.0 79 105.0 80 59.0 81 46.0 82 33.5 83 29.0 84 25.0 85 21.0 86 18.5 87 15.5 88 17.0 89 16.5 90 14.5 91 16.5 92 15.0 93 13.5 94 13.5 95 14.0 96 15.0 97 12.0 98 9.0 99 6.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037418320961928514 2 0.00786710281708391 3 0.006743230986071922 4 0.003173285169916199 5 0.004561597431754536 6 0.003768276139275486 7 0.003768276139275486 8 0.007272111847724623 9 0.01024706669452106 10-11 0.0018510830157844492 12-13 0.006016019801299461 14-15 0.00618129507056593 16-17 0.013949232726089958 18-19 0.007635717440110853 20-21 0.0026113492544102054 22-23 0.007106836578458153 24-25 0.016527526926646868 26-27 0.004594652485607829 28-29 0.01308980132590432 30-31 0.00955291056360189 32-33 0.009519855509748596 34-35 0.22011360360908303 36-37 0.386545799760417 38-39 0.3828105786749948 40-41 0.4016189043175189 42-43 0.3921982139693302 44-45 0.4060482815338603 46-47 0.41123792498882744 48-49 0.41725394479012684 50-51 0.42657546997675566 52-53 0.4161631280129681 54-55 0.4059491163723004 56-57 0.40115613356357277 58-59 0.39923894044008174 60-61 0.39576815978548596 62-63 0.3948426182775937 64-65 0.4078332544419382 66-67 0.3853558178216984 68-69 0.4081638049804711 70-71 0.39259487461556974 72-73 0.3999992066787075 74-75 0.4109073744502945 76-77 0.40148668410210575 78-79 0.4010569684020129 80-81 0.40304027163321055 82-83 0.4027097210946776 84-85 0.4031063817409171 86-87 0.40346998733330336 88-89 0.41507231123580945 90-91 0.4367233715097169 92-93 0.42594742395354307 94-95 0.40135446388669255 96-97 0.3779845408124139 98-99 0.3695224470259707 100-101 0.3787117519971864 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1512628.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.73033856847137 #Duplication Level Percentage of deduplicated Percentage of total 1 72.81174829836856 37.66576391236924 2 14.232357558662533 14.724893502743111 3 5.308782383872002 8.238753303121056 4 2.4806292841050044 5.132951709184665 5 1.3409736110712214 3.4684509456049963 6 0.8435815204106224 2.6183254596568406 7 0.5614628145211592 2.0331263042150485 8 0.3963523045689656 1.6402751126197177 9 0.3031291451337925 1.4112875976968142 >10 1.5748958240531057 14.60424961156577 >50 0.10502347142152166 3.719708076486135 >100 0.03901705538341758 3.5029791805583876 >500 0.0016629668477911757 0.5804758857698087 >1k 3.837615802595021E-4 0.6587593984083954 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4495 0.2971649341411107 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3246 0.21459340961558293 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2214 0.14636777846238466 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.3222021541317496E-4 7 0.0 0.0 0.0 0.0 1.3222021541317496E-4 8 0.0 0.0 0.0 0.0 1.3222021541317496E-4 9 0.0 0.0 0.0 0.0 1.3222021541317496E-4 10-11 0.0 0.0 0.0 0.0 1.3222021541317496E-4 12-13 0.0 0.0 0.0 0.0 3.3055053853293736E-4 14-15 0.0 0.0 0.0 0.0 4.2971570009281857E-4 16-17 0.0 0.0 0.0 3.305505385329374E-5 4.6277075394611235E-4 18-19 0.0 0.0 0.0 6.611010770658748E-5 6.611010770658747E-4 20-21 0.0 0.0 0.0 6.611010770658748E-5 7.272111847724623E-4 22-23 0.0 0.0 0.0 2.6444043082634987E-4 8.263763463323434E-4 24-25 0.0 0.0 0.0 3.9666064623952485E-4 9.255415078922247E-4 26-27 0.0 0.0 0.0 3.9666064623952485E-4 9.585965617455185E-4 28-29 0.0 0.0 0.0 5.619359155059936E-4 9.916516155988122E-4 30-31 0.0 0.0 0.0 0.0012560920464251622 0.001024706669452106 32-33 0.0 0.0 0.0 0.0027105144159700863 0.001256092046425162 34-35 0.0 0.0 0.0 0.004792982808727591 0.0013222021541317494 36-37 0.0 0.0 0.0 0.008726534217269547 0.0013552572079850432 38-39 0.0 0.0 0.0 0.01642836176508699 0.0014544223695449245 40-41 0.0 0.0 0.0 0.02389880393593137 0.0014544223695449245 42-43 0.0 0.0 0.0 0.031997292129988336 0.0014544223695449245 44-45 0.0 0.0 0.0 0.042508799255335745 0.0014874774233982182 46-47 0.0 0.0 0.0 0.055499435419680186 0.0015866425849580994 48-49 0.0 0.0 0.0 0.071233641053848 0.0016527526926646868 50-51 0.0 0.0 0.0 0.0887197645422404 0.001784972908077862 52-53 0.0 0.0 0.0 0.1056108970612735 0.001917193123491037 54-55 0.0 0.0 0.0 0.12418783732682458 0.0019833032311976245 56-57 0.0 0.0 0.0 0.1493757883630344 0.002049413338904212 58-59 0.0 0.0 0.0 0.1785303458616395 0.0021155234466107994 60-61 0.0 0.0 0.0 0.2105606930454811 0.002148578500464093 62-63 0.0 0.0 0.0 0.24629320626089163 0.0022477436620239742 64-65 0.0 0.0 0.0 0.28305042614575426 0.0022477436620239742 66-67 0.0 0.0 0.0 0.32631949163971574 0.0022807987158772677 68-69 0.0 0.0 0.0 0.37699288919681506 0.0023469088235838556 70-71 0.0 0.0 0.0 0.43305426053200125 0.002644404308263499 72-73 0.0 0.0 0.0 0.4924211372525168 0.002908844739089849 74-75 0.0 0.0 0.0 0.5526143903193648 0.0029749548467964365 76-77 0.0 0.0 0.0 0.6185592227566857 0.003041064954503024 78-79 0.0 0.0 0.0 0.7030148853518512 0.003173285169916199 80-81 0.0 0.0 0.0 0.7946104395793282 0.0032724503314760798 82-83 0.0 0.0 0.0 0.8967505559860058 0.0036360559238623113 84-85 0.0 0.0 0.0 1.0014689665932404 0.0037021660315688988 86-87 0.0 0.0 0.0 1.108236790539379 0.003768276139275486 88-89 0.0 0.0 0.0 1.2331848941048293 0.003900496354688661 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2290 0.0 52.758102 1 GTATCAA 4265 0.0 45.21264 1 ATCAACG 5320 0.0 35.79184 3 TCAACGC 5370 0.0 35.458584 4 TATCAAC 5445 0.0 35.056004 2 CAACGCA 5435 0.0 35.034515 5 GTAGGAC 250 0.0 34.103745 3 AACGCAG 5625 0.0 33.935333 6 ACGCAGA 6730 0.0 28.363485 7 GTACATG 6375 0.0 27.944231 1 TACATGG 6265 0.0 27.594786 2 CGCAGAG 6950 0.0 27.5338 8 TAACGCA 105 7.041047E-5 27.066465 4 TACCTGG 1265 0.0 26.958593 2 ACATGGG 6360 0.0 25.917418 3 GTACCTG 1480 0.0 24.329678 1 GTGGTAT 1130 0.0 23.899065 1 GCAGAGT 7940 0.0 23.683159 9 CATGGGA 2830 0.0 22.929983 4 ATAACGC 155 3.9933162E-5 21.39124 3 >>END_MODULE