FastQCFastQC Report
Thu 26 May 2016
SRR936283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512628
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48580.3211629032386019No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37700.24923510605383475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25770.17036574755987594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27900.052.836751
GTATCAA49050.039.5548551
TCAACGC63650.030.187644
ATCAACG63700.030.1639463
CAACGCA65000.029.5606675
TATCAAC65200.029.478762
AACGCAG68250.028.2920446
GTACATG70250.024.9103661
TACATGG70300.024.8424422
ACATGGG68500.024.587273
ACGCAGA81100.023.8677717
CGCAGAG82650.023.3053578
TACCTGG12950.023.0872692
TGGTATC14950.022.5381832
GTACCTG13650.022.2959481
GTGGTAT15800.021.9707261
CATGGGG40350.021.0466064
GTGTAGC8400.020.9461
GCAGAGT94850.019.9575929
GAGTACT51600.019.6300212-13
Produced by FastQC (version 0.11.5)