##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936283_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1512628 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.98422811160444 33.0 31.0 34.0 30.0 34.0 2 32.13871553349535 33.0 31.0 34.0 30.0 34.0 3 32.201896963430535 34.0 31.0 34.0 30.0 34.0 4 35.82696803179632 37.0 35.0 37.0 35.0 37.0 5 35.77268436125736 37.0 35.0 37.0 35.0 37.0 6 35.827705820598325 37.0 35.0 37.0 35.0 37.0 7 35.75897444712117 37.0 35.0 37.0 33.0 37.0 8 35.73497383361937 37.0 35.0 37.0 33.0 37.0 9 37.408338335664816 39.0 37.0 39.0 34.0 39.0 10-11 37.30090610513623 39.0 37.0 39.0 34.0 39.0 12-13 37.22647537927369 39.0 37.0 39.0 33.0 39.0 14-15 38.36249890918322 40.0 38.0 41.0 33.0 41.0 16-17 38.23792961653493 40.0 38.0 41.0 33.0 41.0 18-19 38.26463082793654 40.0 38.0 41.0 33.5 41.0 20-21 38.187406288922325 40.0 38.0 41.0 33.0 41.0 22-23 38.02904712857358 40.0 37.0 41.0 33.0 41.0 24-25 38.21802782971094 40.0 38.0 41.0 33.5 41.0 26-27 38.093565304886596 40.0 37.5 41.0 33.0 41.0 28-29 37.96064531398335 40.0 37.0 41.0 33.0 41.0 30-31 37.791278490150916 40.0 37.0 41.0 33.0 41.0 32-33 37.582974465632 40.0 36.5 41.0 32.0 41.0 34-35 37.380846447375035 39.5 36.0 41.0 31.5 41.0 36-37 37.170840087582675 39.0 36.0 41.0 31.0 41.0 38-39 36.98486045478465 39.0 36.0 41.0 31.0 41.0 40-41 36.782062080035544 39.0 35.0 40.5 30.0 41.0 42-43 36.78219892795849 39.0 35.0 40.5 31.0 41.0 44-45 36.58386992704089 39.0 35.0 41.0 30.0 41.0 46-47 36.29946589643984 39.0 35.0 40.0 30.0 41.0 48-49 35.9725603386953 39.0 35.0 40.0 29.0 41.0 50-51 35.69923107333726 38.5 35.0 40.0 28.0 41.0 52-53 35.3976400674852 38.0 34.0 40.0 27.5 41.0 54-55 35.137098480260846 38.0 34.0 40.0 27.0 41.0 56-57 34.78795513503651 38.0 34.0 40.0 26.0 41.0 58-59 34.4419946609477 37.0 33.5 40.0 26.0 41.0 60-61 34.08510849990877 37.0 33.0 40.0 26.0 41.0 62-63 33.71186041776299 36.0 33.0 39.0 25.0 41.0 64-65 33.25465844873955 36.0 32.0 39.0 23.5 40.0 66-67 32.842431185988886 35.0 31.5 38.5 22.5 40.0 68-69 32.44275525773686 35.0 31.0 38.0 22.0 40.0 70-71 32.02437479671142 35.0 31.0 37.0 21.5 39.5 72-73 31.556622646149613 35.0 30.5 37.0 20.0 39.0 74-75 31.056066329593264 34.0 30.0 36.0 18.5 39.0 76-77 30.48007804959316 34.0 29.5 35.0 18.5 38.0 78-79 30.619789531861105 34.0 30.0 35.0 18.0 37.5 80-81 30.43862172325251 34.0 30.0 35.0 18.0 37.0 82-83 30.06299764383576 34.0 30.0 35.0 16.0 36.5 84-85 29.673019737833755 34.0 29.5 35.0 10.0 36.0 86-87 29.114608813270678 34.0 29.0 35.0 7.0 36.0 88-89 28.491006380947596 33.0 28.0 35.0 2.0 35.0 90-91 28.127065279764754 33.0 27.0 35.0 2.0 35.0 92-93 27.640487614932425 33.0 26.5 35.0 2.0 35.0 94-95 27.23936288367001 33.0 26.0 35.0 2.0 35.0 96-97 26.666200810774363 33.0 25.0 34.5 2.0 35.0 98-99 25.772075487165385 32.0 24.0 34.0 2.0 35.0 100-101 21.952438074662112 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10100049223389362 1101 2 0.07473421409405745 1101 3 0.27035051663999354 1101 4 0.11025777055603925 1101 5 0.08139073501829586 1101 6 0.10122860348280938 1101 7 0.17500005331051227 1101 8 0.10882994883509411 1101 9 0.09531555087284005 1101 10-11 0.07741653775578783 1101 12-13 0.27485849785789185 1101 14-15 0.3587136790480443 1101 16-17 0.33748021536811024 1101 18-19 0.13501676574478694 1101 20-21 0.11578819408952512 1101 22-23 0.2684427168702612 1101 24-25 0.24446533926512615 1101 26-27 0.3356918572733889 1101 28-29 0.3550011694341535 1101 30-31 0.4227277190520411 1101 32-33 0.4412120626183551 1101 34-35 0.2195532242064786 1101 36-37 0.1519886588132593 1101 38-39 0.47025099042540575 1101 40-41 0.5217330539731364 1101 42-43 0.4149571623904933 1101 44-45 0.3408061391880679 1101 46-47 0.5519728952449938 1101 48-49 0.47858535601077534 1101 50-51 0.5614095100618215 1101 52-53 0.4612850206233645 1101 54-55 0.4173624211529514 1101 56-57 0.44118456783451876 1101 58-59 0.14424384472513907 1101 60-61 0.42310930699161275 1101 62-63 0.37209614756971376 1101 64-65 0.38429934815703604 1101 66-67 0.31789149789128857 1101 68-69 0.5872949423959781 1101 70-71 0.6665421801160392 1101 72-73 0.7164674840088878 1101 74-75 0.5235002498261601 1101 76-77 0.5011718988317924 1101 78-79 0.4959769488271917 1101 80-81 0.5497709556608932 1101 82-83 0.374182488153064 1101 84-85 0.6155715468327578 1101 86-87 0.49792316217290633 1101 88-89 0.4843665592001507 1101 90-91 0.6378264242786145 1101 92-93 0.7314339612144707 1101 94-95 0.761275985364513 1101 96-97 0.7011885114242524 1101 98-99 0.8619706346544085 1101 100-101 1.1385560089529427 1102 1 0.23634811935934152 1102 2 0.2686181025246128 1102 3 0.21438658173761382 1102 4 0.2179246936828747 1102 5 0.1938407948075067 1102 6 0.23757271741291675 1102 7 0.2369429090408559 1102 8 0.24291072451896412 1102 9 0.2223220427140049 1102 10-11 0.21909455973720782 1102 12-13 0.27053652601907174 1102 14-15 0.38478378228099075 1102 16-17 0.3537893827377161 1102 18-19 0.4306790355099892 1102 20-21 0.26295075584852867 1102 22-23 0.3737203890770857 1102 24-25 0.20071725112934757 1102 26-27 0.37193925791051896 1102 28-29 0.3263935009660841 1102 30-31 0.17803768052121427 1102 32-33 0.38101962479449725 1102 34-35 0.2582949317368275 1102 36-37 0.2175389512705479 1102 38-39 0.2698829855310336 1102 40-41 0.3154293865927045 1102 42-43 0.2426730910389523 1102 44-45 0.3945021010218568 1102 46-47 0.389080518909779 1102 48-49 0.38588041189128575 1102 50-51 0.3035658457094783 1102 52-53 0.29506020702046953 1102 54-55 0.13765012002545518 1102 56-57 0.3172702749693599 1102 58-59 0.2613789374370299 1102 60-61 0.29108639242992496 1102 62-63 0.5278323582007545 1102 64-65 0.3919653443194022 1102 66-67 0.25105426976224265 1102 68-69 0.04446995637768936 1102 70-71 -0.012200516176406495 1102 72-73 -0.16447397200510494 1102 74-75 0.1813522568587267 1102 76-77 -0.030963641912933326 1102 78-79 0.09746874364669722 1102 80-81 0.2249656954465138 1102 82-83 0.2839046460027568 1102 84-85 0.19622209049741812 1102 86-87 -0.02004836147859379 1102 88-89 0.3600003711561399 1102 90-91 0.46398273520721744 1102 92-93 0.2963886790001382 1102 94-95 0.2561483269076632 1102 96-97 0.24167831944991391 1102 98-99 0.41914812054137585 1102 100-101 0.781035855200436 1103 1 0.1240569528017943 1103 2 0.1023818327930357 1103 3 0.23100535916784537 1103 4 0.15867237196420092 1103 5 0.07970680193633228 1103 6 0.1583099691769405 1103 7 0.19109351159656285 1103 8 0.18110026890440167 1103 9 0.15369494831764996 1103 10-11 0.2159741819578116 1103 12-13 0.27547548239743946 1103 14-15 0.23077442266313852 1103 16-17 0.2826784130716149 1103 18-19 0.13078481616516768 1103 20-21 0.3369744822373164 1103 22-23 0.2331563872342599 1103 24-25 0.283674744885559 1103 26-27 0.31840485040343225 1103 28-29 0.28898382370316966 1103 30-31 0.3420567998442223 1103 32-33 0.41229463025023705 1103 34-35 0.5521681404369048 1103 36-37 0.5763657255970642 1103 38-39 0.7570847386408062 1103 40-41 0.6324409770003072 1103 42-43 0.546271148797004 1103 44-45 0.516795449873733 1103 46-47 0.4621631308735985 1103 48-49 0.6132572941201389 1103 50-51 0.657881029628399 1103 52-53 0.48201463344078377 1103 54-55 0.5694716083501774 1103 56-57 0.772260454644929 1103 58-59 0.8612386037884789 1103 60-61 0.6168210981839124 1103 62-63 0.8566707714303092 1103 64-65 0.7735304130616782 1103 66-67 0.6531207812435369 1103 68-69 0.6620774343728826 1103 70-71 0.3821870471707456 1103 72-73 0.43986545962846435 1103 74-75 0.6223924231980504 1103 76-77 0.5207506715130279 1103 78-79 0.15922312642112146 1103 80-81 0.37728983889599377 1103 82-83 0.6636420432530663 1103 84-85 0.6153938491841622 1103 86-87 0.6230131549734814 1103 88-89 0.8352904344426761 1103 90-91 1.073489961026187 1103 92-93 1.2472673555598703 1103 94-95 1.1122608726752254 1103 96-97 0.8963256970595701 1103 98-99 0.712819770113402 1103 100-101 1.2392343106210113 1104 1 0.08762341633254778 1104 2 0.17334053907120506 1104 3 0.08040706043109225 1104 4 0.10324642135161355 1104 5 0.14437305598318062 1104 6 0.04881891985300513 1104 7 0.08252688936588015 1104 8 0.19142766686034918 1104 9 0.1948339377164956 1104 10-11 0.2330661644373393 1104 12-13 0.3151226924526611 1104 14-15 0.37136272330428 1104 16-17 0.4057938514008157 1104 18-19 0.48350731346726405 1104 20-21 0.3536677311422878 1104 22-23 0.31164937269734594 1104 24-25 0.234860630113765 1104 26-27 0.47692822690314784 1104 28-29 0.5624158683930673 1104 30-31 0.45532887500450414 1104 32-33 0.3277432749528302 1104 34-35 0.3127673120232757 1104 36-37 0.27093183998797343 1104 38-39 0.3294499230501202 1104 40-41 0.42950939254020426 1104 42-43 0.4516800391670941 1104 44-45 0.42065503217551026 1104 46-47 0.5178886276043855 1104 48-49 0.3557269824334739 1104 50-51 0.302218616119859 1104 52-53 0.3590003663170549 1104 54-55 0.30212967348062847 1104 56-57 0.4709031734921254 1104 58-59 0.47472294010537297 1104 60-61 0.3386538443275029 1104 62-63 0.30219702039875784 1104 64-65 0.2554445722694396 1104 66-67 0.14753057620323062 1104 68-69 0.45250822533333945 1104 70-71 0.3748848666337068 1104 72-73 0.467562564941737 1104 74-75 0.482278238598294 1104 76-77 0.5445635960353385 1104 78-79 0.4978491024256897 1104 80-81 0.5056681636595322 1104 82-83 0.4160602334390582 1104 84-85 0.6821470073127749 1104 86-87 0.6423249527262875 1104 88-89 0.925047147449721 1104 90-91 1.007312090144449 1104 92-93 0.7332858917117164 1104 94-95 0.6811108536312318 1104 96-97 0.43735161275816736 1104 98-99 0.36783786190544276 1104 100-101 0.30663020346682046 1105 1 0.03485182186336999 1105 2 0.06697634415761655 1105 3 0.051779323090194396 1105 4 0.06233254797007248 1105 5 -0.06562240959051024 1105 6 0.09622673948098281 1105 7 0.14130463573637542 1105 8 0.16455530212758163 1105 9 0.13742388031968744 1105 10-11 0.044554261077543345 1105 12-13 0.13810340052982895 1105 14-15 0.17172184594186746 1105 16-17 0.24400316523023236 1105 18-19 0.27680617730884904 1105 20-21 0.2252141569936228 1105 22-23 0.02618662565944163 1105 24-25 0.13131891928061634 1105 26-27 0.20476572759017841 1105 28-29 0.16040315236985947 1105 30-31 0.04069337613909596 1105 32-33 0.007462611631041227 1105 34-35 0.08474136764557016 1105 36-37 0.2680963513299375 1105 38-39 0.26955157468055546 1105 40-41 0.3501105573577661 1105 42-43 0.4906748785581101 1105 44-45 0.4387417005695795 1105 46-47 0.49568834140553264 1105 48-49 0.25975908852726803 1105 50-51 0.3161227179108579 1105 52-53 0.34054147256004086 1105 54-55 0.393596020578201 1105 56-57 0.6208373654220267 1105 58-59 0.5897523549877093 1105 60-61 0.5109377524140939 1105 62-63 0.3580195378494295 1105 64-65 0.42623712521034207 1105 66-67 0.39810621278956404 1105 68-69 0.4543644447603086 1105 70-71 0.4035903860708885 1105 72-73 0.24901205025490825 1105 74-75 0.35694527233756546 1105 76-77 0.4437629066455031 1105 78-79 0.2900364429355875 1105 80-81 0.3276445748279606 1105 82-83 0.3967965280864725 1105 84-85 0.4993340533794033 1105 86-87 0.604669339459857 1105 88-89 0.576354671849252 1105 90-91 0.8077615087171459 1105 92-93 0.9416118639633808 1105 94-95 0.7142555960522614 1105 96-97 0.615692709578969 1105 98-99 0.5352941272655656 1105 100-101 0.511166200137648 1106 1 0.13725963399740948 1106 2 0.10203429191099644 1106 3 0.09080359184017084 1106 4 0.021177357578523015 1106 5 0.013106739204715723 1106 6 0.04073321007795272 1106 7 0.10415456262214917 1106 8 0.08637504885971481 1106 9 0.14632882416326964 1106 10-11 0.0865256102637133 1106 12-13 0.03645714501752906 1106 14-15 0.010546768864195144 1106 16-17 0.0825502876238744 1106 18-19 0.11616897953067706 1106 20-21 0.08116236922747788 1106 22-23 0.01282418074656988 1106 24-25 0.15546313798817835 1106 26-27 -0.072984509617946 1106 28-29 0.11046180180905907 1106 30-31 0.04599577398872867 1106 32-33 0.17790904187030065 1106 34-35 0.10918565882075626 1106 36-37 0.15353093433457587 1106 38-39 0.2793658362891378 1106 40-41 0.017515790257590425 1106 42-43 0.16039016654385563 1106 44-45 -0.03793981884604847 1106 46-47 0.15370559281836194 1106 48-49 0.047193547958571 1106 50-51 -0.10971843095717304 1106 52-53 -0.059144517049261935 1106 54-55 -5.147998002854592E-4 1106 56-57 0.1760293854581505 1106 58-59 0.2859192824516015 1106 60-61 0.13556361976242925 1106 62-63 0.15100449974046626 1106 64-65 0.1279741927575344 1106 66-67 0.17896901333501347 1106 68-69 0.22583327673840614 1106 70-71 0.07871488141988436 1106 72-73 -0.15391031551355283 1106 74-75 -0.07170214083659943 1106 76-77 0.11500298425722377 1106 78-79 0.1106663891620272 1106 80-81 0.06820498470827019 1106 82-83 -0.06326246141889058 1106 84-85 0.08788608517894403 1106 86-87 0.2307924558645027 1106 88-89 0.13416663825071495 1106 90-91 0.34262092488580365 1106 92-93 0.49413053287735664 1106 94-95 0.45503178030873315 1106 96-97 0.502485641375582 1106 98-99 0.5043351742095474 1106 100-101 0.8357289397106697 1107 1 0.0886538164165529 1107 2 0.09455203916409971 1107 3 0.04465556001509441 1107 4 -0.021219446708705902 1107 5 0.07151857008264528 1107 6 0.08335322546128765 1107 7 -0.008226065019862006 1107 8 0.11984439478221987 1107 9 0.09029199876707139 1107 10-11 -0.012995495996108275 1107 12-13 0.01352898006403791 1107 14-15 0.1448700820066975 1107 16-17 0.19071133861799439 1107 18-19 0.05067869611171005 1107 20-21 0.10851656877425597 1107 22-23 0.07029241504825023 1107 24-25 0.14243485324965377 1107 26-27 0.1460079953180795 1107 28-29 0.10721883529910059 1107 30-31 -0.001040421193359009 1107 32-33 0.039319095436034956 1107 34-35 0.14285554981194792 1107 36-37 0.039741563814502 1107 38-39 -0.10301595914601336 1107 40-41 0.028097375884172493 1107 42-43 -0.23487159897214838 1107 44-45 -0.2906633144993407 1107 46-47 -0.21705395068784838 1107 48-49 -0.26721726590455575 1107 50-51 -0.20641786317318633 1107 52-53 -0.22473339768750122 1107 54-55 -0.10249344544601513 1107 56-57 -0.21733163656288212 1107 58-59 -0.17471056703119814 1107 60-61 -0.21349394159968682 1107 62-63 -0.0022198165708502415 1107 64-65 -0.06809827743047947 1107 66-67 -0.09103708185539716 1107 68-69 -0.049356440374786814 1107 70-71 0.15402004745099873 1107 72-73 0.15531145628981236 1107 74-75 0.14630192820806442 1107 76-77 -0.05453150974095777 1107 78-79 0.04540350512684199 1107 80-81 0.20719922408812863 1107 82-83 0.4040637209470397 1107 84-85 0.18055268910341837 1107 86-87 0.30220078976859455 1107 88-89 0.06634372496650087 1107 90-91 -0.17710024355776355 1107 92-93 0.13379144910717145 1107 94-95 0.2749083095880671 1107 96-97 0.18554904367440983 1107 98-99 -0.011671968545531541 1107 100-101 0.2994613248813742 1108 1 -0.006019638922701631 1108 2 0.10222531274909841 1108 3 0.05564620658797992 1108 4 -0.037853539132413516 1108 5 -0.031548020193163495 1108 6 -0.04410278261413936 1108 7 0.09325864305465359 1108 8 0.05703470293916979 1108 9 0.14091747670682508 1108 10-11 0.13280791268335435 1108 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-1.1558331347832151 2316 62-63 -1.145185911548161 2316 64-65 -1.082659700472803 2316 66-67 -0.9999981760973142 2316 68-69 -0.8224479569483663 2316 70-71 -0.962613835395068 2316 72-73 -1.024847436418895 2316 74-75 -1.0051535955335176 2316 76-77 -0.7719381835279684 2316 78-79 -1.1536657642681867 2316 80-81 -1.1376446438253751 2316 82-83 -1.4334455975847789 2316 84-85 -0.9570930797676667 2316 86-87 -1.003022536549782 2316 88-89 -0.9205273972756842 2316 90-91 -0.8724792139854323 2316 92-93 -1.0632544331212515 2316 94-95 -0.8995712615784797 2316 96-97 -0.7254558273919294 2316 98-99 -0.8272055888002967 2316 100-101 -0.6828356410917245 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 22.0 5 33.0 6 78.0 7 230.0 8 498.0 9 877.0 10 1443.0 11 2118.0 12 3079.0 13 4629.0 14 5635.0 15 7665.0 16 9689.0 17 12928.0 18 12152.0 19 9055.0 20 8659.0 21 9605.0 22 10372.0 23 11551.0 24 13752.0 25 16605.0 26 20089.0 27 24794.0 28 30066.0 29 36775.0 30 44896.0 31 54167.0 32 67464.0 33 85861.0 34 116352.0 35 165056.0 36 240435.0 37 293994.0 38 175444.0 39 16547.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10143439228807 19.067148110113624 13.76744956438202 23.063967933216283 2 16.675901522494918 20.02339349874966 38.963201193346144 24.337503785409282 3 20.737683025833185 23.957509711574822 28.658401140267138 26.646406122324855 4 13.70971580586899 15.769574541790842 35.39667386826106 35.1240357840791 5 14.20236832849848 37.541682422909 33.31889929315073 14.937049955441786 6 34.45215875945705 34.946464034779204 16.11876813069704 14.482609075066705 7 30.36351303823544 31.808349442162907 19.395978389927993 18.432159129673654 8 27.65035421795709 35.47798930074017 18.295773977474962 18.575882503827774 9 27.114069024241257 15.754501437233742 19.156461469707025 37.97496806881798 10-11 26.469556720785626 26.03640229283606 25.919949068655175 21.574091917723138 12-13 26.55572061056691 22.953808176106865 27.256389050307845 23.23408216301838 14-15 23.289367908038198 24.435122184701065 25.08690173658031 27.188608170680435 16-17 23.20709973892963 27.818769961239415 25.764055588353706 23.210074711477247 18-19 23.163031492210905 26.254835954378734 27.996705072231904 22.585427481178453 20-21 23.848204531518828 25.472894020504057 28.18926007890244 22.489641369074672 22-23 23.45167597442199 25.276282495194618 28.180500486735827 23.09154104364756 24-25 23.234388387615432 25.560131218464726 28.29632842167617 22.90915197224367 26-27 23.059174697829395 25.854928359990016 28.203074512552085 22.8828224296285 28-29 22.962248228138947 25.927927624174064 28.319167369923864 22.790656777763125 30-31 23.16674974580428 25.754819140904424 28.136590035527902 22.941841077763396 32-33 23.008172650379542 25.72649453988679 28.181132429727242 23.084200380006425 34-35 23.280219700305302 25.88747499348806 28.068644445414076 22.763660860792566 36-37 23.508067914936095 25.69694124958061 27.960221535044155 22.834769300439138 38-39 23.161020358310765 25.886975989760852 28.04752399057136 22.904479661357023 40-41 23.193185867444654 25.831784696893674 28.093124450870377 22.881904984791298 42-43 23.269019464729844 25.765016375971683 27.837267665488547 23.128696493809922 44-45 23.248497758455294 25.9655897599653 27.800178900456373 22.98573358112303 46-47 23.41480540887394 26.248140009474753 27.481378606298897 22.85567597535242 48-49 23.507368936082994 26.36264157680025 27.178580165693617 22.951409321423142 50-51 23.384837426741292 26.49468590282463 27.05546687065113 23.065009799782946 52-53 23.669675760945598 26.29505211811235 27.02561807201992 23.009654048922133 54-55 23.583009668622427 26.38443103875713 26.958201801504007 23.074357491116437 56-57 23.387908778680206 26.70349104347855 26.8818611846607 23.026738993180548 58-59 23.484668181932374 26.40879382998613 27.064747858993023 23.041790129088476 60-61 23.41110624093867 26.456142995314718 27.170901402807996 22.961849360938615 62-63 23.342322104311172 26.51078123636479 27.291938269025827 22.85495839029821 64-65 23.293381642496108 26.455150455980803 27.161739724854616 23.089728176668473 66-67 23.282624677052123 26.715292854555116 26.874254608535608 23.12782785985715 68-69 23.317762199298176 26.50691379506395 27.19594639263586 22.979377613002008 70-71 23.281366312624534 26.38097586581217 27.114501535739834 23.223156285823464 72-73 23.37024968118 26.652723869816985 26.821769538591926 23.155256910411083 74-75 23.259221698923994 26.894186806009145 26.758165259402837 23.088426235664024 76-77 23.428896075517873 26.711448458891862 26.728035534083954 23.131619931506307 78-79 23.36944116014368 26.815883826838576 26.680247902770876 23.134427110246865 80-81 23.381858569431085 26.85564403367678 26.613117019201848 23.14938037769029 82-83 23.523695184804193 26.887344409861512 26.618606822034234 22.970353583300056 84-85 23.385328054638915 26.87470064388216 26.497177251546404 23.24279404993252 86-87 23.417464767108203 26.731798545154216 26.685507395995465 23.165229291742108 88-89 23.475260180022016 26.859569072841104 26.443653162412872 23.221517584724012 90-91 23.211710381388865 27.02066029002167 26.61070943573089 23.156919892858575 92-93 23.402959233319677 26.887303348592166 26.502219888985667 23.207517529102486 94-95 23.369330053752122 26.68883171695729 26.623050572477762 23.318787656812827 96-97 23.364885122728033 26.660187724056545 26.579002606452168 23.395924546763254 98-99 23.22368299268278 26.72237001816744 26.497392562191095 23.556554426958687 100-101 23.19845936393891 26.875782581504172 26.335793079830914 23.589964974726005 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 133.0 1 111.0 2 147.0 3 250.5 4 223.5 5 196.5 6 441.5 7 910.5 8 1432.0 9 1612.0 10 1391.5 11 1201.5 12 1176.5 13 1297.5 14 1432.5 15 1529.0 16 1602.5 17 1703.0 18 1865.5 19 1958.0 20 2146.5 21 2501.5 22 2950.5 23 3583.0 24 4365.0 25 5331.5 26 6942.0 27 8728.5 28 10110.0 29 11909.0 30 13995.5 31 16526.0 32 19673.5 33 22058.5 34 24170.5 35 26545.5 36 30143.5 37 33526.5 38 35448.0 39 37051.5 40 38591.5 41 40895.5 42 42244.5 43 44168.5 44 47590.0 45 50675.5 46 53093.5 47 55070.0 48 56426.5 49 56886.0 50 56767.5 51 56381.5 52 56371.5 53 56972.5 54 57411.5 55 55390.5 56 51640.0 57 47482.0 58 44668.0 59 41458.0 60 35673.5 61 29810.5 62 24374.5 63 18869.5 64 14175.0 65 10583.5 66 7736.5 67 6127.0 68 4627.0 69 3362.5 70 2630.5 71 1769.5 72 1252.5 73 999.0 74 741.0 75 541.5 76 375.5 77 230.5 78 135.0 79 71.5 80 43.5 81 27.0 82 14.0 83 7.5 84 4.5 85 2.0 86 2.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21670893306219374 2 0.01599864606499417 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.3138537697305615E-4 12-13 6.611010770658747E-5 14-15 0.0 16-17 5.949909693592873E-4 18-19 0.0 20-21 3.305505385329373E-5 22-23 3.305505385329373E-5 24-25 0.002379963877437149 26-27 0.002016358285050918 28-29 0.0015535875311048055 30-31 9.255415078922246E-4 32-33 0.0014544223695449243 34-35 0.0014544223695449243 36-37 3.305505385329373E-5 38-39 1.6527526926646868E-4 40-41 4.627707539461123E-4 42-43 0.003041064954503024 44-45 0.0036360559238623113 46-47 0.012395645194985153 48-49 0.01308980132590432 50-51 0.006247405178272516 52-53 0.006743230986071922 54-55 1.9833032311976243E-4 56-57 0.0028427346313832616 58-59 0.0014544223695449243 60-61 0.001256092046425162 62-63 0.0 64-65 6.28046023212581E-4 66-67 0.0 68-69 0.0 70-71 1.3222021541317493E-4 72-73 0.001256092046425162 74-75 0.0 76-77 0.15972202021911536 78-79 0.08124932237139601 80-81 8.594314001856373E-4 82-83 0.0 84-85 4.297157000928186E-4 86-87 0.10180956586814471 88-89 0.5127830504261457 90-91 0.27465444246701765 92-93 0.2640437701801104 94-95 0.0024130189312904426 96-97 0.002346908823583855 98-99 0.002115523446610799 100-101 3.9666064623952485E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1512628.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.43952140378113 #Duplication Level Percentage of deduplicated Percentage of total 1 73.29540052341034 38.43575724546086 2 14.134144699536863 14.823755669910058 3 5.128168774402949 8.067561486225165 4 2.4317452248141156 5.100782230607295 5 1.338058122070162 3.5083563765900716 6 0.8173994494739532 2.5718421555676962 7 0.546973454799326 2.0078118333174553 8 0.3819475207768775 1.6023316152720162 9 0.28711173375909865 1.3550401716963263 >10 1.5024034585116317 14.341267516056346 >50 0.09808423011154635 3.5485294297989025 >100 0.036919198229614064 3.4157827672568746 >500 0.0010114523713720756 0.3397217013672026 >1k 6.321577321075473E-4 0.8814598008737233 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4858 0.3211629032386019 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3770 0.24923510605383475 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2577 0.17036574755987594 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 6.611010770658748E-5 5 0.0 0.0 0.0 0.0 6.611010770658748E-5 6 0.0 0.0 0.0 0.0 1.3222021541317496E-4 7 0.0 0.0 0.0 0.0 1.3222021541317496E-4 8 0.0 0.0 0.0 0.0 1.3222021541317496E-4 9 0.0 0.0 0.0 0.0 1.3222021541317496E-4 10-11 0.0 0.0 0.0 0.0 1.3222021541317496E-4 12-13 0.0 0.0 0.0 0.0 3.6360559238623113E-4 14-15 0.0 0.0 0.0 0.0 4.6277075394611235E-4 16-17 0.0 0.0 0.0 0.0 5.288808616526998E-4 18-19 0.0 0.0 0.0 0.0 5.949909693592873E-4 20-21 0.0 0.0 0.0 0.0 6.611010770658747E-4 22-23 0.0 0.0 0.0 1.9833032311976243E-4 8.92486454038931E-4 24-25 0.0 0.0 0.0 3.3055053853293736E-4 9.916516155988122E-4 26-27 0.0 0.0 0.0 3.3055053853293736E-4 0.0011569268848652808 28-29 0.0 0.0 0.0 4.95825807799406E-4 0.001256092046425162 30-31 0.0 0.0 0.0 0.0011899819387185746 0.001256092046425162 32-33 0.0 0.0 0.0 0.002677459362116793 0.001388312261838337 34-35 0.0 0.0 0.0 0.004792982808727591 0.001388312261838337 36-37 0.0 0.0 0.0 0.008693479163416253 0.001388312261838337 38-39 0.0 0.0 0.0 0.016461416818940283 0.001388312261838337 40-41 0.0 0.0 0.0 0.02389880393593137 0.001388312261838337 42-43 0.0 0.0 0.0 0.03193118202228175 0.001388312261838337 44-45 0.0 0.0 0.0 0.042343523986069276 0.001388312261838337 46-47 0.0 0.0 0.0 0.055069719719587365 0.001388312261838337 48-49 0.0 0.0 0.0 0.07027504449210248 0.0014213673156916308 50-51 0.0 0.0 0.0 0.08733145228040205 0.001520532477251512 52-53 0.0 0.0 0.0 0.10385897920704892 0.0015866425849580994 54-55 0.0 0.0 0.0 0.1224359194726 0.0016858077465179806 56-57 0.0 0.0 0.0 0.14709498964715714 0.0017849729080778617 58-59 0.0 0.0 0.0 0.17598510671493586 0.0018510830157844494 60-61 0.0 0.0 0.0 0.20755268314483138 0.001884138069637743 62-63 0.0 0.0 0.0 0.2421943795830832 0.0022477436620239742 64-65 0.0 0.0 0.0 0.27845577366014646 0.0022477436620239742 66-67 0.0 0.0 0.0 0.32079929764621573 0.0022477436620239742 68-69 0.0 0.0 0.0 0.3700843829414767 0.0022477436620239742 70-71 0.0 0.0 0.0 0.42564992846886346 0.0023469088235838556 72-73 0.0 0.0 0.0 0.4835293277659808 0.0028096795775299677 74-75 0.0 0.0 0.0 0.5422681584632838 0.002908844739089849 76-77 0.0 0.0 0.0 0.6061635775617006 0.002908844739089849 78-79 0.0 0.0 0.0 0.6877103954177762 0.002941899792943143 80-81 0.0 0.0 0.0 0.7760996094214836 0.0029749548467964365 82-83 0.0 0.0 0.0 0.8742400643119128 0.003371615493035961 84-85 0.0 0.0 0.0 0.9745952078105126 0.003371615493035961 86-87 0.0 0.0 0.0 1.0767353242171902 0.0034377256007425486 88-89 0.0 0.0 0.0 1.19646072927382 0.003503835708449136 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2790 0.0 52.83675 1 GTATCAA 4905 0.0 39.554855 1 TCAACGC 6365 0.0 30.18764 4 ATCAACG 6370 0.0 30.163946 3 CAACGCA 6500 0.0 29.560667 5 TATCAAC 6520 0.0 29.47876 2 AACGCAG 6825 0.0 28.292044 6 GTACATG 7025 0.0 24.910366 1 TACATGG 7030 0.0 24.842442 2 ACATGGG 6850 0.0 24.58727 3 ACGCAGA 8110 0.0 23.867771 7 CGCAGAG 8265 0.0 23.305357 8 TACCTGG 1295 0.0 23.087269 2 TGGTATC 1495 0.0 22.538183 2 GTACCTG 1365 0.0 22.295948 1 GTGGTAT 1580 0.0 21.970726 1 CATGGGG 4035 0.0 21.046606 4 GTGTAGC 840 0.0 20.946 1 GCAGAGT 9485 0.0 19.957592 9 GAGTACT 5160 0.0 19.63002 12-13 >>END_MODULE