FastQCFastQC Report
Thu 26 May 2016
SRR936282_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936282_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386962
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35220.25393630106664783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25470.1836387730882317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18150.13086155208289774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25050.039.501661
GTATCAA41900.035.819711
TCAACGC50750.029.189624
ATCAACG51800.028.7806723
CAACGCA51750.028.7170245
TATCAAC52800.028.1469652
AACGCAG53000.028.0367056
TATAGCG856.545038E-427.8401285
GTACATG52900.026.8498821
TACATGG51500.026.7437082
ACATGGG51050.025.6805443
ACGCAGA63000.023.4362017
CGCAGAG65050.022.7711988
CATGGGG33850.022.0911644
TACCTGG12450.021.2889632
TATAACG1150.003801843820.578232
GCAGAGT72600.019.8164469
ACCGTAT1200.004866902719.718678
AGAGTAC59050.019.71710610-11
GAGTACT35650.019.38476212-13
Produced by FastQC (version 0.11.5)