##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936282_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1386962 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48263110308718 33.0 31.0 34.0 30.0 34.0 2 31.580765010144475 33.0 31.0 34.0 30.0 34.0 3 31.64245667869776 34.0 31.0 34.0 29.0 34.0 4 35.1304412089156 37.0 35.0 37.0 33.0 37.0 5 35.10468635766517 37.0 35.0 37.0 32.0 37.0 6 35.18743123459763 37.0 35.0 37.0 32.0 37.0 7 35.12304518797198 37.0 35.0 37.0 32.0 37.0 8 35.117985207958114 37.0 35.0 37.0 32.0 37.0 9 36.7532809118058 39.0 37.0 39.0 33.0 39.0 10-11 36.66589820052749 39.0 37.0 39.0 32.5 39.0 12-13 36.556447112465946 39.0 37.0 39.0 32.0 39.0 14-15 37.750900168858266 40.0 38.0 41.0 32.0 41.0 16-17 37.682994919831984 40.0 38.0 41.0 32.0 41.0 18-19 37.619653242121984 40.0 38.0 41.0 32.0 41.0 20-21 37.529121201590236 40.0 38.0 41.0 32.0 41.0 22-23 37.35351220869786 40.0 37.5 41.0 32.0 41.0 24-25 37.510560130702935 40.0 38.0 41.0 32.0 41.0 26-27 37.4487992461221 40.0 38.0 41.0 32.0 41.0 28-29 37.30172383958609 40.0 37.5 41.0 31.5 41.0 30-31 37.13520990481354 40.0 37.0 41.0 31.0 41.0 32-33 36.93426171733616 40.0 37.0 41.0 30.5 41.0 34-35 36.65112850964914 40.0 36.0 41.0 30.0 41.0 36-37 36.43853184153568 40.0 36.0 41.0 30.0 41.0 38-39 36.260508218682276 40.0 36.0 41.0 29.5 41.0 40-41 36.06232506730538 40.0 35.0 41.0 29.0 41.0 42-43 35.84885382584382 39.0 35.0 41.0 28.0 41.0 44-45 35.64398628080654 39.0 35.0 41.0 27.0 41.0 46-47 35.34644604538553 39.0 35.0 41.0 26.0 41.0 48-49 35.1485188491105 39.0 35.0 41.0 25.5 41.0 50-51 34.830072849869 38.5 34.5 40.5 25.0 41.0 52-53 34.84017947139143 39.0 35.0 40.0 25.0 41.0 54-55 34.892326898646104 39.0 35.0 41.0 25.0 41.0 56-57 34.697330568537566 38.5 34.5 41.0 24.5 41.0 58-59 34.42805246286488 38.0 34.0 40.0 23.5 41.0 60-61 34.10492176425886 37.5 34.0 40.0 23.0 41.0 62-63 33.77621881493509 37.0 34.0 40.0 22.0 41.0 64-65 33.42054288437607 36.5 33.0 40.0 22.0 41.0 66-67 33.03567869920012 36.0 33.0 39.0 20.5 41.0 68-69 32.632247314634434 35.5 33.0 39.0 19.5 41.0 70-71 32.21891263062723 35.0 32.0 38.5 18.0 40.0 72-73 31.811656339539223 35.0 32.0 37.0 15.5 39.5 74-75 31.398186467978213 35.0 32.0 37.0 11.5 39.0 76-77 31.005403536650608 35.0 31.0 36.0 7.5 39.0 78-79 30.620092331296746 35.0 31.0 36.0 6.5 38.0 80-81 30.23365492349466 35.0 31.0 35.0 2.0 37.0 82-83 29.88778459683827 34.0 30.5 35.0 2.0 37.0 84-85 29.568980620954285 34.0 30.0 35.0 2.0 36.0 86-87 29.24739178146193 34.0 30.0 35.0 2.0 36.0 88-89 28.91623851266293 34.0 29.5 35.0 2.0 36.0 90-91 28.54975839280384 34.0 29.0 35.0 2.0 35.0 92-93 28.2166447242246 34.0 29.0 35.0 2.0 35.0 94-95 27.8076190984324 34.0 28.5 35.0 2.0 35.0 96-97 27.350419477966952 34.0 27.0 35.0 2.0 35.0 98-99 26.807720038472574 34.0 26.5 35.0 2.0 35.0 100-101 23.516182130440487 29.5 13.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2467221414344074 1101 2 0.15154312380790458 1101 3 0.2051123486065194 1101 4 0.013383482316022821 1101 5 0.24542114060461273 1101 6 0.1452011593330269 1101 7 0.1388743584361336 1101 8 0.14004139124904924 1101 9 0.33923902264562145 1101 10-11 0.4834356397469506 1101 12-13 0.31687043493361955 1101 14-15 0.3811895957042353 1101 16-17 0.3496549015979511 1101 18-19 0.3201208738048251 1101 20-21 0.40503708670166105 1101 22-23 0.4473960572692164 1101 24-25 0.5536351649699895 1101 26-27 0.39500215296943253 1101 28-29 0.3631045063897318 1101 30-31 0.3948109332212226 1101 32-33 0.3935524082237123 1101 34-35 0.197442176206593 1101 36-37 0.32185038731215343 1101 38-39 0.41009891043536584 1101 40-41 0.29964036189532095 1101 42-43 0.31661918742675255 1101 44-45 0.509632165679605 1101 46-47 0.4500993677385168 1101 48-49 0.5380360408156548 1101 50-51 0.6518753521215359 1101 52-53 0.7993472837424491 1101 54-55 0.751205528783693 1101 56-57 0.7232350129640039 1101 58-59 0.9673356788006942 1101 60-61 0.6803254838624753 1101 62-63 0.7253432279482155 1101 64-65 0.5875589971286459 1101 66-67 0.7116668038261764 1101 68-69 0.6998451880418344 1101 70-71 0.8361845652497522 1101 72-73 0.791749454896177 1101 74-75 0.651525169650153 1101 76-77 0.5099296748161066 1101 78-79 0.7554664698091891 1101 80-81 0.685033590279545 1101 82-83 0.7380309771655611 1101 84-85 0.7089756254575015 1101 86-87 0.8460720191231346 1101 88-89 0.6049934699014443 1101 90-91 0.5776212921872066 1101 92-93 0.6755087105728563 1101 94-95 1.1200418685045612 1101 96-97 1.2640773705899022 1101 98-99 1.2104129154403367 1101 100-101 1.2872445028292283 1102 1 0.2969926211678029 1102 2 0.3559762013867598 1102 3 0.30768384360768053 1102 4 0.379859495646258 1102 5 0.4570928448594387 1102 6 0.34326052537193874 1102 7 0.2565710181276799 1102 8 0.24345920106072327 1102 9 0.3172193373011254 1102 10-11 0.4219352134894834 1102 12-13 0.5536293282142495 1102 14-15 0.6358854028808594 1102 16-17 0.6406771444872064 1102 18-19 0.5736650108272201 1102 20-21 0.4376264070147684 1102 22-23 0.5120957395136543 1102 24-25 0.6271777519652559 1102 26-27 0.5775240471025782 1102 28-29 0.409430167088793 1102 30-31 0.47402506946860967 1102 32-33 0.2553690400147701 1102 34-35 0.3407871347433655 1102 36-37 0.4042033284886273 1102 38-39 0.3805631435594492 1102 40-41 0.32141669298111 1102 42-43 0.31791036002226747 1102 44-45 0.23579662355761144 1102 46-47 0.3416331195791713 1102 48-49 0.1365511148294516 1102 50-51 0.12633245512084557 1102 52-53 0.31977664125620464 1102 54-55 0.33332596589134056 1102 56-57 0.280150458984302 1102 58-59 0.3161723834430248 1102 60-61 0.2733627620148411 1102 62-63 0.46793022322062683 1102 64-65 0.2784231372510959 1102 66-67 0.38347646049517436 1102 68-69 0.4221237279162864 1102 70-71 0.42987285479481585 1102 72-73 0.28148672529840724 1102 74-75 0.48948223389877654 1102 76-77 0.43993974999241914 1102 78-79 0.5499948740562637 1102 80-81 0.5075895850094554 1102 82-83 0.37148521261468304 1102 84-85 0.41198732816230077 1102 86-87 0.4428712131090258 1102 88-89 0.2651515726682412 1102 90-91 0.2552451006038545 1102 92-93 0.35870486650558675 1102 94-95 0.545911821228735 1102 96-97 0.9107409372023163 1102 98-99 0.8178468455341132 1102 100-101 0.6986620026354764 1103 1 0.2694213487769197 1103 2 0.14759771585748993 1103 3 0.279666255275572 1103 4 0.2607027504488073 1103 5 0.28080465571278523 1103 6 0.12427798155128045 1103 7 0.30769371596637995 1103 8 0.24066717347683664 1103 9 0.3979814983593073 1103 10-11 0.30564077712104876 1103 12-13 0.35911967505520437 1103 14-15 0.29081740427447045 1103 16-17 0.4399764344664234 1103 18-19 0.3608452912313638 1103 20-21 0.4454060002245157 1103 22-23 0.45065221079445195 1103 24-25 0.45809669426255084 1103 26-27 0.4178366484697591 1103 28-29 0.502452500906692 1103 30-31 0.4678397192037025 1103 32-33 0.3280797936459692 1103 34-35 0.11673653224688252 1103 36-37 0.2500239971816427 1103 38-39 0.27350107981376226 1103 40-41 0.307548461305295 1103 42-43 0.2890761276496434 1103 44-45 0.44952429274319883 1103 46-47 0.594104076004804 1103 48-49 0.5435843452438078 1103 50-51 0.6828238591839408 1103 52-53 0.7240761114437362 1103 54-55 0.7970127878575326 1103 56-57 0.6127168056810817 1103 58-59 0.6888086509682836 1103 60-61 0.5594380650651729 1103 62-63 0.6755503320664573 1103 64-65 0.6541875208785726 1103 66-67 0.6237938674175751 1103 68-69 0.6055460641369521 1103 70-71 0.5723818952455773 1103 72-73 0.6449239587402644 1103 74-75 0.7737407367280476 1103 76-77 0.7702661072614276 1103 78-79 0.7830336669007956 1103 80-81 0.8073081596817744 1103 82-83 0.9816021077454558 1103 84-85 0.9300469050331621 1103 86-87 0.9057612139499973 1103 88-89 0.8227290322114698 1103 90-91 0.8166441779372491 1103 92-93 0.7154806993683778 1103 94-95 0.7428575905380548 1103 96-97 0.9234462245144535 1103 98-99 1.0180450746870164 1103 100-101 1.0139794095578765 1104 1 0.337733384055511 1104 2 0.30150011114141506 1104 3 0.4024354775555281 1104 4 0.4010664344681416 1104 5 0.3666812634131915 1104 6 0.38978363982901243 1104 7 0.381260523189475 1104 8 0.3395341384235664 1104 9 0.41913163929893926 1104 10-11 0.4259475794698844 1104 12-13 0.45620477683981875 1104 14-15 0.5102032707657855 1104 16-17 0.4778525225525385 1104 18-19 0.32560751021430434 1104 20-21 0.5115349657322454 1104 22-23 0.5134157541040736 1104 24-25 0.5964888329122005 1104 26-27 0.5415347387722846 1104 28-29 0.5731050996678917 1104 30-31 0.6071273877909462 1104 32-33 0.6239754155506958 1104 34-35 0.5015212601851147 1104 36-37 0.4985301060983147 1104 38-39 0.48003453975517374 1104 40-41 0.6804866731883692 1104 42-43 0.5408804077999179 1104 44-45 0.6644273363953914 1104 46-47 0.6789466282766199 1104 48-49 0.6738368180100451 1104 50-51 0.6387250451000881 1104 52-53 0.5688913494776671 1104 54-55 0.7471608549383646 1104 56-57 0.7008138447993062 1104 58-59 0.6661945082628833 1104 60-61 0.6822141848799461 1104 62-63 0.6355164651704897 1104 64-65 0.582201398718631 1104 66-67 0.7359903184059888 1104 68-69 0.7094465051193524 1104 70-71 0.7531484642738135 1104 72-73 0.769712434339084 1104 74-75 0.6925435316091573 1104 76-77 0.7669834554811708 1104 78-79 0.7542042705270582 1104 80-81 0.7820028288070304 1104 82-83 0.948278967435801 1104 84-85 0.8352569579602509 1104 86-87 0.6325170963976134 1104 88-89 0.6092899903250419 1104 90-91 0.8110083171938776 1104 92-93 0.7954461197271385 1104 94-95 0.48145654027342033 1104 96-97 0.8078079036503283 1104 98-99 1.0236516209073976 1104 100-101 1.3109338030576403 1105 1 0.2420796258916056 1105 2 0.254325883253145 1105 3 0.271129206569217 1105 4 0.2085885277488302 1105 5 0.29806640602785706 1105 6 0.3227035505334115 1105 7 0.40969812696793895 1105 8 0.19305430373111676 1105 9 0.34591824311429775 1105 10-11 0.25950480521801467 1105 12-13 0.22212958127508386 1105 14-15 0.32230718298304595 1105 16-17 0.3558442248882443 1105 18-19 0.230552784373927 1105 20-21 0.288433189045044 1105 22-23 0.316773448469597 1105 24-25 0.23327230031192414 1105 26-27 0.24759839878483092 1105 28-29 0.23027123283640805 1105 30-31 0.434740990610031 1105 32-33 0.34844360860630985 1105 34-35 -0.07403349332951592 1105 36-37 7.734504398513309E-4 1105 38-39 0.15216610316611678 1105 40-41 0.20209911368871758 1105 42-43 0.24543524433731534 1105 44-45 0.23427946362317442 1105 46-47 0.11650658916367718 1105 48-49 0.1745834463632363 1105 50-51 0.04006401783172464 1105 52-53 -0.037024368577014855 1105 54-55 0.058015966373368144 1105 56-57 0.22998596944415084 1105 58-59 0.1843096147165184 1105 60-61 0.22543906588064289 1105 62-63 0.06461271623133058 1105 64-65 0.302391613101868 1105 66-67 0.19146474739385155 1105 68-69 0.02139647929003985 1105 70-71 0.11075923048169756 1105 72-73 -0.13516769001056872 1105 74-75 -3.4833584960836106E-4 1105 76-77 0.14238992110974635 1105 78-79 0.1395440642616066 1105 80-81 0.1737734276779186 1105 82-83 0.09951531480305675 1105 84-85 0.12304096500795936 1105 86-87 0.2287941018586679 1105 88-89 0.2694599333160781 1105 90-91 0.26186806406668595 1105 92-93 0.1528718143509522 1105 94-95 0.06288919036013141 1105 96-97 0.21212704777785163 1105 98-99 0.10104210004100622 1105 100-101 0.10146736270488432 1106 1 0.1789478966013398 1106 2 0.05817622749983187 1106 3 0.21570411684534818 1106 4 0.2220356639093808 1106 5 0.13471631813508367 1106 6 0.1487994635195662 1106 7 0.10475141196024396 1106 8 0.19027103265516132 1106 9 0.2757802697751046 1106 10-11 0.19199331506933248 1106 12-13 0.2569605250237075 1106 14-15 0.2733561740472865 1106 16-17 0.3345153502819258 1106 18-19 0.23867943236337652 1106 20-21 0.35928986889562253 1106 22-23 0.27242078788218294 1106 24-25 0.3453928049629127 1106 26-27 0.37047203461577993 1106 28-29 0.16567119072112035 1106 30-31 0.21799146669587088 1106 32-33 0.161511249417849 1106 34-35 0.054545161544872656 1106 36-37 0.22492404920934916 1106 38-39 0.378055101920971 1106 40-41 0.0777422873501834 1106 42-43 0.1652227910303523 1106 44-45 0.24840181768455238 1106 46-47 0.17851219232781546 1106 48-49 0.14569899789037777 1106 50-51 0.39919296385091485 1106 52-53 0.3316251498438447 1106 54-55 0.42182909352490583 1106 56-57 0.4164491094133922 1106 58-59 0.26621926827251485 1106 60-61 0.3821308186089851 1106 62-63 0.1347850226841203 1106 64-65 0.08641343980661276 1106 66-67 0.10267016012365104 1106 68-69 0.24189517920947168 1106 70-71 0.37200332518616364 1106 72-73 0.4474171814462551 1106 74-75 0.34537696261959283 1106 76-77 0.369060570417588 1106 78-79 0.21511582504678017 1106 80-81 0.29213569238164894 1106 82-83 0.20209121387285833 1106 84-85 0.17942165972706547 1106 86-87 0.18107908501254855 1106 88-89 0.20565413056210247 1106 90-91 0.37517649444829004 1106 92-93 0.14646525535293264 1106 94-95 -0.059338982935113194 1106 96-97 0.08335841634153596 1106 98-99 0.1756513892082232 1106 100-101 0.2954082548165111 1107 1 -0.09741417976073663 1107 2 -0.07828880896520474 1107 3 0.1091833103245392 1107 4 0.03908843096215264 1107 5 0.0022838857026528103 1107 6 0.08196119668130564 1107 7 0.007389764598599413 1107 8 0.11165670404083272 1107 9 0.13901493300976142 1107 10-11 0.1418109898870057 1107 12-13 -0.0037644857013034994 1107 14-15 0.09748744817855481 1107 16-17 -0.23410326833668904 1107 18-19 -0.06749817381422218 1107 20-21 0.10312298772873874 1107 22-23 0.25891799937939197 1107 24-25 0.20922806879818268 1107 26-27 0.11471270385644772 1107 28-29 0.046559221609150825 1107 30-31 0.1468417455461548 1107 32-33 0.094780232686837 1107 34-35 -0.03807603107632218 1107 36-37 0.056981106266405845 1107 38-39 0.0812940551599226 1107 40-41 0.2536646382725394 1107 42-43 -0.20861890781134917 1107 44-45 0.040304434587163485 1107 46-47 0.12107975989538033 1107 48-49 -0.07888272669134722 1107 50-51 -0.08996405030610077 1107 52-53 -0.006395013176323516 1107 54-55 0.14154166323747575 1107 56-57 -0.1190292260649457 1107 58-59 -0.041459839406591925 1107 60-61 -0.11865210717393637 1107 62-63 0.10277086566996729 1107 64-65 -0.10596107756790474 1107 66-67 -0.05320714575365315 1107 68-69 0.060974973289269485 1107 70-71 -0.011812633629794789 1107 72-73 -0.01080890177983207 1107 74-75 -0.056789489546861915 1107 76-77 -0.2002337238767069 1107 78-79 0.053972538903497735 1107 80-81 -0.05758545733950271 1107 82-83 0.035832097613742064 1107 84-85 -0.2481952428898353 1107 86-87 -0.5343434804100156 1107 88-89 -0.23714938724141632 1107 90-91 -0.0628222185504228 1107 92-93 -0.402376443488766 1107 94-95 -0.2814754050715358 1107 96-97 -0.14997491699179832 1107 98-99 -0.1514078128509766 1107 100-101 0.003645063053316022 1108 1 0.11129827672123582 1108 2 0.009346170677208221 1108 3 0.053238305308902056 1108 4 -0.04025599578004346 1108 5 0.04144125868901938 1108 6 -0.016743588826457767 1108 7 -0.06573822265578144 1108 8 0.06214941661533402 1108 9 -0.0630031232833872 1108 10-11 2.2840889922548513E-4 1108 12-13 -0.003947408932553742 1108 14-15 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2316 56-57 -0.9359994046242193 2316 58-59 -1.149729748717526 2316 60-61 -1.1811437235387956 2316 62-63 -1.2751694817284331 2316 64-65 -1.0862709561631405 2316 66-67 -1.1099155118925133 2316 68-69 -1.2262590589233326 2316 70-71 -1.0562597511173308 2316 72-73 -1.0694457470057763 2316 74-75 -0.969026923814301 2316 76-77 -1.389682250786958 2316 78-79 -1.6055303722834324 2316 80-81 -1.3234130314799977 2316 82-83 -1.3548299908591268 2316 84-85 -1.4719741482057884 2316 86-87 -1.528052351997026 2316 88-89 -1.3747860769448508 2316 90-91 -1.6677243365725651 2316 92-93 -1.7206360008328296 2316 94-95 -1.6243993740059146 2316 96-97 -1.6800456797324905 2316 98-99 -1.2623123706324506 2316 100-101 -0.8626417884907305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16927.0 3 3599.0 4 2858.0 5 2438.0 6 2293.0 7 2514.0 8 2942.0 9 3462.0 10 3969.0 11 4679.0 12 5801.0 13 7725.0 14 7914.0 15 7231.0 16 8364.0 17 9914.0 18 9547.0 19 7472.0 20 7500.0 21 7498.0 22 8030.0 23 8992.0 24 10168.0 25 11793.0 26 13667.0 27 16280.0 28 19249.0 29 23220.0 30 28270.0 31 34911.0 32 43531.0 33 56606.0 34 77874.0 35 116826.0 36 190918.0 37 299937.0 38 257414.0 39 44586.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.232687979687235 17.058904149114202 12.513705349414275 24.194702521784293 2 16.10058457773326 18.371186309906236 39.89175180792219 25.636477304438305 3 20.637221181482428 22.528146843818366 27.46507512795331 29.369556846745898 4 13.192447573423166 14.268306400209966 34.734932496546236 37.80431352982063 5 14.759613859301496 36.63664183344726 33.00150268377592 15.602241623475324 6 35.78418047402769 33.50864277468651 16.00822276749051 14.698953983795294 7 31.49425867507886 30.757205948625504 18.79260928346102 18.95592609283461 8 28.017600053071284 34.99027266814439 17.970758876100184 19.02136840268414 9 27.18890121190182 15.885533471883098 18.707896038640097 38.217669277574984 10-11 26.87266818873174 25.37587472777134 25.61424266447189 22.13721441902502 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25.16241151397666 26.921121030624928 23.81733703069447 38-39 23.779611918437244 25.42121127170708 26.91126946148558 23.8879073483701 40-41 23.942660380126757 25.35184276163375 26.95338891716584 23.75210794107365 42-43 23.93078027050073 25.353303451568433 26.80437055223791 23.911545725692932 44-45 23.863579551423943 25.428233989906857 26.840259537947098 23.8679269207221 46-47 24.159915192646164 25.69088936857505 26.459039366872233 23.69015607190655 48-49 24.16711595374952 25.665162279595698 26.37670258064703 23.791019186007752 50-51 23.97051368414797 25.924527467764968 26.360596785175527 23.744362062911534 52-53 24.183656351416815 25.858463839499063 26.1911499409955 23.76672986808862 54-55 24.089125514846828 25.79025297007159 26.25379172528074 23.866829789800843 56-57 23.914707549852647 26.017819978010316 26.216741505261044 23.850730966875997 58-59 24.146682784910915 25.709625989835654 26.221103718148562 23.922587507104865 60-61 23.884172440874142 25.814040090532618 26.418448194419085 23.88333927417415 62-63 23.9320623273956 25.90902570700792 26.450637676371635 23.70827428922485 64-65 23.7937328452471 25.9142559438411 26.34234582822495 23.949665382686852 66-67 23.81882377144778 26.03560733270262 26.184363604226462 23.96120529162314 68-69 23.926274346699977 25.81767341900652 26.442487969077366 23.813564265216133 70-71 23.920813339674794 25.767722075860654 26.249055029092606 24.062409555371943 72-73 23.890163449231984 25.952306688561816 26.193392403861864 23.964137458344336 74-75 23.72805024829224 26.334049985978535 26.025360373384 23.912539392345224 76-77 24.01458336201269 26.024939809578733 25.958753893449003 24.001722934959567 78-79 24.00799300394796 26.089238788094832 26.0077292753913 23.895038932565914 80-81 24.051931133320874 26.208289679417234 25.89354675569892 23.846232431562978 82-83 24.033538067605058 26.249037251397116 25.947297529349793 23.77012715164803 84-85 23.948119942022203 26.29103304017183 25.924016625325546 23.836830392480422 86-87 24.01234646171347 26.24709950530829 25.944234423369885 23.796319609608357 88-89 23.94045872995629 26.292261359551993 25.732580957460378 24.034698953031338 90-91 23.837789999826043 26.387809714885453 25.85872855262471 23.915671732663796 92-93 23.889397000802994 26.316678213932672 25.89651958638563 23.897405198878708 94-95 23.89414802221286 26.13282287276802 26.046023323575024 23.927005781444098 96-97 24.015900886022404 25.947990369069764 26.00727824812104 24.028830496786792 98-99 23.824280328682192 26.180789691923817 25.78529187022196 24.20963810917203 100-101 23.9139197833906 26.235350489062508 25.593287310596253 24.257442416950642 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 83.0 1 69.0 2 140.0 3 251.5 4 210.5 5 170.5 6 342.5 7 743.5 8 1204.5 9 1359.5 10 1188.0 11 1036.5 12 1060.0 13 1177.0 14 1339.5 15 1559.0 16 1680.0 17 1741.0 18 1801.5 19 1875.5 20 1984.0 21 2089.5 22 2335.0 23 2734.5 24 3191.5 25 3844.0 26 4864.0 27 6125.0 28 7310.0 29 8562.0 30 9910.0 31 11795.5 32 14465.0 33 16132.0 34 17383.5 35 19282.0 36 21824.0 37 24351.0 38 26829.5 39 28864.0 40 30754.5 41 33484.5 42 35323.5 43 37922.5 44 41121.5 45 43878.0 46 46800.5 47 49547.0 48 52456.0 49 54006.5 50 53781.0 51 54329.0 52 56081.5 53 57473.5 54 58319.0 55 56950.5 56 53236.5 57 49566.0 58 46806.0 59 43097.0 60 37323.0 61 31463.0 62 26087.0 63 20292.5 64 15021.5 65 11410.0 66 8599.5 67 6563.0 68 5130.5 69 4052.5 70 3183.0 71 2306.5 72 1770.5 73 1523.0 74 1250.5 75 971.5 76 722.0 77 446.5 78 286.5 79 200.0 80 147.0 81 90.0 82 52.5 83 36.5 84 25.0 85 25.5 86 22.0 87 13.5 88 15.5 89 13.0 90 9.0 91 11.5 92 11.0 93 10.0 94 13.0 95 15.5 96 13.0 97 11.0 98 10.0 99 7.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046288218422710935 2 0.010742904275675902 3 0.00922880367306386 4 0.005047002008706799 5 0.007931003156539256 6 0.005840102324360726 7 0.006272702496535594 8 0.01067080424698009 9 0.015573606198295266 10-11 0.0021990508752222485 12-13 0.009373003730455484 14-15 0.00890435354393271 16-17 0.019539107776564896 18-19 0.010887104333067525 20-21 0.004362051736096591 22-23 0.010094004017413599 24-25 0.022134708809614104 26-27 0.006597152625666744 28-29 0.01791685713090914 30-31 0.013951355552639511 32-33 0.011788354691765169 34-35 0.2651478555288465 36-37 0.4699479870392988 38-39 0.46713608592016215 40-41 0.48772064411281635 42-43 0.4782394903393172 44-45 0.49150589561934643 46-47 0.4969854978002281 48-49 0.5045560008132883 50-51 0.5144697547589624 52-53 0.5034384503685032 54-55 0.4916140456623902 56-57 0.4880811442562954 58-59 0.48635074356759594 60-61 0.4820607918601952 62-63 0.4785999904827963 64-65 0.49590399736979096 66-67 0.4698037869819072 68-69 0.495507447211964 70-71 0.477915040210186 72-73 0.4864949436249876 74-75 0.5000136990054522 76-77 0.4873240939549894 78-79 0.4870356938402061 80-81 0.48941499478716793 82-83 0.4902080951028219 84-85 0.4887300445145577 86-87 0.4909651454041279 88-89 0.503114000239372 90-91 0.5267628096515983 92-93 0.5137848044863522 94-95 0.48815324428499124 96-97 0.4621611839401512 98-99 0.4540138806975245 100-101 0.4623414340118907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1386962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.12482932923832 #Duplication Level Percentage of deduplicated Percentage of total 1 79.57355197804102 50.230668877351135 2 12.037333049265188 15.197091886281298 3 3.75328665799117 7.107767391281998 4 1.5779514253141809 3.984316576511441 5 0.8789486360308849 2.7741741319308213 6 0.48993772612579267 1.8556341206187463 7 0.3307105597542636 1.4613233349305332 8 0.22900733587531275 1.156483919381814 9 0.16966869321490072 0.9639276571534954 >10 0.8873975578842822 9.860365014913802 >50 0.05198813926736829 2.2049862697152456 >100 0.018733318449389972 2.173324015613821 >500 0.0011422482971556918 0.4614670643812233 >1k 3.426744891467076E-4 0.5684697399346195 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3522 0.25393630106664783 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2547 0.1836387730882317 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1815 0.13086155208289774 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.210002869581142E-5 2 0.0 0.0 0.0 0.0 7.210002869581142E-5 3 0.0 0.0 0.0 0.0 7.210002869581142E-5 4 0.0 0.0 0.0 0.0 7.210002869581142E-5 5 0.0 0.0 0.0 0.0 7.210002869581142E-5 6 0.0 0.0 0.0 0.0 1.4420005739162285E-4 7 0.0 0.0 0.0 0.0 1.4420005739162285E-4 8 0.0 0.0 0.0 0.0 1.4420005739162285E-4 9 0.0 0.0 0.0 0.0 1.4420005739162285E-4 10-11 0.0 0.0 0.0 0.0 1.4420005739162285E-4 12-13 0.0 0.0 0.0 0.0 2.884001147832457E-4 14-15 0.0 0.0 0.0 0.0 2.884001147832457E-4 16-17 0.0 0.0 0.0 0.0 3.605001434790571E-4 18-19 0.0 0.0 0.0 0.0 4.3260017217486854E-4 20-21 0.0 0.0 0.0 7.210002869581142E-5 4.3260017217486854E-4 22-23 3.605001434790571E-5 0.0 0.0 3.244501291311514E-4 5.768002295664914E-4 24-25 7.210002869581142E-5 0.0 0.0 3.9655015782696283E-4 5.768002295664914E-4 26-27 7.210002869581142E-5 0.0 0.0 5.768002295664914E-4 7.210002869581142E-4 28-29 7.210002869581142E-5 0.0 0.0 9.012503586976428E-4 7.210002869581142E-4 30-31 7.210002869581142E-5 0.0 0.0 0.0024874509900054942 7.5705030130602E-4 32-33 7.210002869581142E-5 0.0 0.0 0.004794651908271459 8.652003443497371E-4 34-35 7.210002869581142E-5 0.0 0.0 0.007570503013060199 0.00100940040174136 36-37 7.210002869581142E-5 0.0 0.0 0.012797755093506526 0.00100940040174136 38-39 7.210002869581142E-5 0.0 0.0 0.020548508178306255 0.0010454504160892658 40-41 7.210002869581142E-5 0.0 0.0 0.03291366309963791 0.0010815004304371714 42-43 7.210002869581142E-5 0.0 0.0 0.04380076743270544 0.0010815004304371714 44-45 1.4420005739162285E-4 0.0 0.0 0.05587752223925385 0.001117550444785077 46-47 2.1630008608743427E-4 0.0 0.0 0.07152322846624493 0.0011536004591329828 48-49 2.1630008608743427E-4 0.0 0.0 0.08774573492280249 0.0011536004591329828 50-51 2.1630008608743427E-4 0.0 0.0 0.10634754232632185 0.0012257004878287942 52-53 2.1630008608743427E-4 0.0 0.0 0.12473304964375376 0.0014420005739162285 54-55 2.5235010043534E-4 0.0 0.0 0.14506525773597256 0.0014420005739162285 56-57 3.605001434790571E-4 0.0 0.0 0.17163411831037909 0.00151410060261204 58-59 3.605001434790571E-4 0.0 7.210002869581142E-5 0.2019882303913157 0.00151410060261204 60-61 3.605001434790571E-4 0.0 7.210002869581142E-5 0.23645204410791357 0.00151410060261204 62-63 3.605001434790571E-4 0.0 7.210002869581142E-5 0.26990645742277003 0.0015862006313078513 64-65 3.605001434790571E-4 0.0 7.210002869581142E-5 0.30685772212937346 0.0015862006313078513 66-67 4.3260017217486854E-4 0.0 7.210002869581142E-5 0.3479907885003338 0.0016222506456557572 68-69 4.3260017217486854E-4 0.0 7.210002869581142E-5 0.3964059577695712 0.001874600746091097 70-71 4.3260017217486854E-4 0.0 7.210002869581142E-5 0.45304053031013103 0.001874600746091097 72-73 4.3260017217486854E-4 0.0 7.210002869581142E-5 0.5138208545007001 0.0019467007747869085 74-75 4.3260017217486854E-4 0.0 7.210002869581142E-5 0.5745290786625733 0.00201880080348272 76-77 4.3260017217486854E-4 0.0 7.210002869581142E-5 0.6385539041444539 0.00201880080348272 78-79 4.3260017217486854E-4 0.0 7.210002869581142E-5 0.7191617362263709 0.00201880080348272 80-81 5.768002295664914E-4 0.0 7.210002869581142E-5 0.8084576217661334 0.0020548508178306257 82-83 7.210002869581142E-4 0.0 7.210002869581142E-5 0.9033412595298214 0.002090900832178531 84-85 7.210002869581142E-4 0.0 7.210002869581142E-5 0.9966747466765491 0.002090900832178531 86-87 7.210002869581142E-4 0.0 7.210002869581142E-5 1.097722936893729 0.002199050875222248 88-89 7.210002869581142E-4 0.0 7.210002869581142E-5 1.2164356341413824 0.0023072009182659656 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2505 0.0 39.50166 1 GTATCAA 4190 0.0 35.81971 1 TCAACGC 5075 0.0 29.18962 4 ATCAACG 5180 0.0 28.780672 3 CAACGCA 5175 0.0 28.717024 5 TATCAAC 5280 0.0 28.146965 2 AACGCAG 5300 0.0 28.036705 6 TATAGCG 85 6.545038E-4 27.840128 5 GTACATG 5290 0.0 26.849882 1 TACATGG 5150 0.0 26.743708 2 ACATGGG 5105 0.0 25.680544 3 ACGCAGA 6300 0.0 23.436201 7 CGCAGAG 6505 0.0 22.771198 8 CATGGGG 3385 0.0 22.091164 4 TACCTGG 1245 0.0 21.288963 2 TATAACG 115 0.0038018438 20.57823 2 GCAGAGT 7260 0.0 19.816446 9 ACCGTAT 120 0.0048669027 19.71867 8 AGAGTAC 5905 0.0 19.717106 10-11 GAGTACT 3565 0.0 19.384762 12-13 >>END_MODULE