FastQCFastQC Report
Thu 26 May 2016
SRR936282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386962
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36830.2655444056866735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27100.19539107776564896No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18870.13605275414899615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25950.042.8993641
GTATCAA42000.034.5480041
ATCAACG53150.027.043163
TCAACGC53450.026.8913754
TATCAAC54450.026.578452
CAACGCA54250.026.494825
GTACATG52600.026.319621
TACATGG51600.026.0234222
AACGCAG56200.025.8287376
TAACGCA1151.2935257E-424.7497864
GTATAGG4050.024.6681541
ACATGGG51400.024.4568673
CCTATAC2151.6880222E-924.270143
GATAGCG1852.5751433E-723.0775037
ACGCAGA65200.022.1179077
CGCAGAG66450.021.7018458
GAGTACA37150.021.6421431
GTACCTG13400.021.3019261
AGAGTAC59350.020.3023710-11
GTAGGAC3052.1282176E-1020.2190883
Produced by FastQC (version 0.11.5)