##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936282_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1386962 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.4408635564637 34.0 31.0 34.0 31.0 34.0 2 32.587552506845896 34.0 31.0 34.0 31.0 34.0 3 32.66832256399238 34.0 31.0 34.0 31.0 34.0 4 36.16070303295981 37.0 35.0 37.0 35.0 37.0 5 36.11813878101923 37.0 35.0 37.0 35.0 37.0 6 36.15673681038125 37.0 36.0 37.0 35.0 37.0 7 36.10296244597905 37.0 36.0 37.0 35.0 37.0 8 36.08008799087502 37.0 36.0 37.0 35.0 37.0 9 37.84288322246752 39.0 38.0 39.0 35.0 39.0 10-11 37.773750109952545 39.0 38.0 39.0 35.0 39.0 12-13 37.71506681509659 39.0 38.0 39.0 35.0 39.0 14-15 39.052859775538195 40.0 38.0 41.0 36.0 41.0 16-17 38.97319429083133 40.0 38.0 41.0 35.0 41.0 18-19 38.98862045247094 40.0 38.5 41.0 35.0 41.0 20-21 38.937523522634365 40.0 38.0 41.0 35.0 41.0 22-23 38.81743659883977 40.0 38.0 41.0 35.0 41.0 24-25 38.97041771872625 40.0 38.0 41.0 35.0 41.0 26-27 38.887362451170254 40.0 38.0 41.0 35.0 41.0 28-29 38.789369499669064 40.0 38.0 41.0 35.0 41.0 30-31 38.666445079245136 40.0 38.0 41.0 34.5 41.0 32-33 38.50403183360467 40.0 38.0 41.0 34.0 41.0 34-35 38.364495206069094 40.0 38.0 41.0 34.0 41.0 36-37 38.216111544512394 40.0 38.0 41.0 33.5 41.0 38-39 38.07134261789436 40.0 38.0 41.0 33.0 41.0 40-41 37.91900535126413 40.0 37.0 41.0 33.0 41.0 42-43 37.9332753889436 40.0 37.0 41.0 33.0 41.0 44-45 37.78768524299873 40.0 37.0 41.0 33.0 41.0 46-47 37.571760798060794 40.0 37.0 41.0 33.0 41.0 48-49 37.31391739643912 40.0 37.0 41.0 32.0 41.0 50-51 37.089489113616665 40.0 36.0 41.0 31.5 41.0 52-53 36.8551268888405 39.5 36.0 41.0 31.0 41.0 54-55 36.63532382285888 39.0 35.0 41.0 31.0 41.0 56-57 36.35025184540024 39.0 35.0 41.0 30.5 41.0 58-59 36.05770237396554 38.5 35.0 40.5 30.0 41.0 60-61 35.74897870309353 38.0 35.0 40.0 29.0 41.0 62-63 35.41924256035854 37.5 35.0 40.0 29.0 41.0 64-65 35.01769587054295 37.0 34.0 40.0 28.0 41.0 66-67 34.64846116908755 36.0 34.0 39.0 28.0 41.0 68-69 34.26819696574239 36.0 34.0 39.0 28.0 41.0 70-71 33.88653798734212 35.0 34.0 38.5 27.0 40.0 72-73 33.47887685459298 35.0 33.0 37.5 26.0 40.0 74-75 33.04748363689849 35.0 33.0 37.0 26.0 39.0 76-77 32.36239168773189 35.0 32.0 36.0 26.0 39.0 78-79 32.4311444725955 35.0 33.0 36.0 26.0 38.5 80-81 32.2783962358017 35.0 33.0 36.0 26.0 37.0 82-83 32.00180394271797 35.0 33.0 35.0 25.0 37.0 84-85 31.713856976615077 35.0 32.0 35.0 25.0 36.5 86-87 31.317869559512083 35.0 32.0 35.0 24.0 36.0 88-89 30.820453985040686 34.0 31.5 35.0 20.5 36.0 90-91 30.590366210465753 34.0 31.0 35.0 19.5 35.0 92-93 30.287485525919237 34.0 31.0 35.0 18.0 35.0 94-95 30.110656600541326 34.0 31.0 35.0 17.0 35.0 96-97 29.82022218344843 34.0 31.0 35.0 4.5 35.0 98-99 29.316041823784644 34.0 31.0 35.0 2.0 35.0 100-101 25.90494872966959 30.5 24.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14089781930463374 1101 2 0.11800895765579611 1101 3 0.19979377964479283 1101 4 0.1921621881251241 1101 5 0.11299567274553368 1101 6 0.1474854938251653 1101 7 0.20968784713215882 1101 8 0.12335474665566437 1101 9 0.07841317073872744 1101 10-11 0.12164264760021126 1101 12-13 0.2561326563201263 1101 14-15 0.3177028474877446 1101 16-17 0.24176496603528363 1101 18-19 0.26370859901784627 1101 20-21 0.2853804809792422 1101 22-23 0.22533669629478936 1101 24-25 0.2320035505175042 1101 26-27 0.27718636744182845 1101 28-29 0.37496651485903953 1101 30-31 0.30625397716381286 1101 32-33 0.38853446548400683 1101 34-35 0.35708967593201635 1101 36-37 0.37718629775728374 1101 38-39 0.3330378266582201 1101 40-41 0.29963322892054833 1101 42-43 0.2860295292738897 1101 44-45 0.28132015053000003 1101 46-47 0.2437338891493468 1101 48-49 0.21785682958476116 1101 50-51 0.3413979000879692 1101 52-53 0.3534160878300838 1101 54-55 0.5060645617638784 1101 56-57 0.46816377202177506 1101 58-59 0.44960926050020333 1101 60-61 0.5132748724854608 1101 62-63 0.4668682568798701 1101 64-65 0.4340142150574948 1101 66-67 0.4575979207794276 1101 68-69 0.47249306210612474 1101 70-71 0.5005734362105656 1101 72-73 0.3889645237214623 1101 74-75 0.24818854017683378 1101 76-77 0.35208823898232566 1101 78-79 0.2588581125877667 1101 80-81 0.2976011800667706 1101 82-83 0.28012925533281674 1101 84-85 0.4497086514525286 1101 86-87 0.47466290058666516 1101 88-89 0.44416738899808905 1101 90-91 0.6366056835222054 1101 92-93 0.7318533988773872 1101 94-95 0.6172393692076241 1101 96-97 0.2266964563753362 1101 98-99 0.1399343444326142 1101 100-101 0.262991823772353 1102 1 0.06331586162037439 1102 2 0.16079900648165335 1102 3 0.12057809337027692 1102 4 0.06540096980380383 1102 5 0.11415199298113521 1102 6 0.18950982925103688 1102 7 0.178324598275303 1102 8 0.17326872015020456 1102 9 0.11037163001176253 1102 10-11 0.19164342261377243 1102 12-13 0.2105541086955398 1102 14-15 0.26227077742651517 1102 16-17 0.2816006631599848 1102 18-19 0.2140286796192612 1102 20-21 0.17580596342519073 1102 22-23 0.25119114874770787 1102 24-25 0.13543531057330682 1102 26-27 0.15939360606849817 1102 28-29 0.12508044185276646 1102 30-31 0.08494420424278815 1102 32-33 -0.018431356417877964 1102 34-35 -0.027820810001486507 1102 36-37 -0.060584763236285255 1102 38-39 -0.05091939259311573 1102 40-41 -0.05497887679129576 1102 42-43 -9.378426551194252E-4 1102 44-45 0.03267874930548231 1102 46-47 0.12828941887111966 1102 48-49 0.08467849896397439 1102 50-51 0.1501447031570251 1102 52-53 0.11619528646606625 1102 54-55 -0.06417181737275257 1102 56-57 0.013113008633411027 1102 58-59 -0.32442933517521766 1102 60-61 -0.1093880741161044 1102 62-63 0.05390227997528285 1102 64-65 -0.008729332954594327 1102 66-67 -0.052736110066113895 1102 68-69 -0.025222197910927946 1102 70-71 0.04306742985546208 1102 72-73 0.019483007794939056 1102 74-75 0.05319202773787168 1102 76-77 -0.08505196097117818 1102 78-79 0.034491686175307734 1102 80-81 0.01072525973816596 1102 82-83 0.1185001768239502 1102 84-85 0.15513684644593795 1102 86-87 0.08229368412537852 1102 88-89 0.16399843604141395 1102 90-91 0.37707224168697806 1102 92-93 0.1738513838421234 1102 94-95 0.05890409834038479 1102 96-97 0.07236955565457492 1102 98-99 0.17815956337394567 1102 100-101 0.44389083950513 1103 1 0.00638324751847108 1103 2 -0.013625227933331985 1103 3 0.0903349322760576 1103 4 -0.028926672951222088 1103 5 0.09240505351911565 1103 6 0.059370588288487625 1103 7 0.07166770290425006 1103 8 0.11592402968803128 1103 9 0.06347962285148157 1103 10-11 0.10902181203194061 1103 12-13 0.13200130165059676 1103 14-15 0.15022445187417333 1103 16-17 0.24457055635652125 1103 18-19 0.08712417801540084 1103 20-21 0.22018917041246766 1103 22-23 0.18020438805234562 1103 24-25 0.1551807362854305 1103 26-27 0.08670928299102343 1103 28-29 0.27825991732640887 1103 30-31 0.1947973945307595 1103 32-33 0.1862762856163087 1103 34-35 0.1085245051828636 1103 36-37 0.034100893382763786 1103 38-39 0.14982666985507365 1103 40-41 -0.005385246554745038 1103 42-43 -0.002491538727781517 1103 44-45 -0.04160642435527251 1103 46-47 0.1307420541174693 1103 48-49 0.1204627655761712 1103 50-51 -0.009449587843370466 1103 52-53 -0.021511202234876237 1103 54-55 0.025616978475248686 1103 56-57 0.059516951591476186 1103 58-59 -0.05487312819995083 1103 60-61 -0.15968001159583167 1103 62-63 -0.0512183394607888 1103 64-65 0.08469005986350453 1103 66-67 -0.05520796753334167 1103 68-69 -0.0767849043996236 1103 70-71 -0.4222571853997792 1103 72-73 -0.2936601857367833 1103 74-75 -0.12502619252475 1103 76-77 -0.17855184683923397 1103 78-79 -0.4101567273907776 1103 80-81 -0.3597073603706811 1103 82-83 -0.15107194855298545 1103 84-85 -0.30306743770654165 1103 86-87 -0.4382755939600891 1103 88-89 -0.3203871136540357 1103 90-91 -0.11227714620542883 1103 92-93 -0.008703249313803951 1103 94-95 -0.008212087970992599 1103 96-97 -0.11120896111080825 1103 98-99 -0.07047986380387172 1103 100-101 0.2652085828163955 1104 1 0.014924694805209526 1104 2 0.05257156333347268 1104 3 0.033891255246224716 1104 4 0.030179452519533356 1104 5 0.11634294811558732 1104 6 -0.017307007138910535 1104 7 -0.0827704009174397 1104 8 0.007572954362352391 1104 9 0.0865001453167551 1104 10-11 0.09362478992763812 1104 12-13 0.09065402916578336 1104 14-15 0.1480896648470562 1104 16-17 0.12093052030540719 1104 18-19 0.28679631519357685 1104 20-21 0.275376921310297 1104 22-23 0.15134452756647931 1104 24-25 0.24637304415459482 1104 26-27 0.3230538884290439 1104 28-29 0.20786942488840765 1104 30-31 0.14800681748300093 1104 32-33 0.09565647313728931 1104 34-35 0.07632968661102524 1104 36-37 0.059688448390609494 1104 38-39 0.10332349009152608 1104 40-41 0.2074594577479374 1104 42-43 0.06728104954916603 1104 44-45 0.17311096065132148 1104 46-47 0.2408784413726579 1104 48-49 0.21332715084488285 1104 50-51 0.39097874906902064 1104 52-53 0.4378686404093628 1104 54-55 0.30011932133790964 1104 56-57 0.3510717249154851 1104 58-59 0.44499236987103785 1104 60-61 0.24564442545037224 1104 62-63 0.33959081034907257 1104 64-65 0.09500647277752705 1104 66-67 0.13368792107606708 1104 68-69 0.22999387786359193 1104 70-71 0.15639942772669002 1104 72-73 0.02187099341978893 1104 74-75 0.10700228049898897 1104 76-77 0.03224486441616392 1104 78-79 0.16564373152520062 1104 80-81 0.1397500335567301 1104 82-83 0.05865851877798889 1104 84-85 0.13709644772080765 1104 86-87 0.25014034118468587 1104 88-89 0.21345219217806033 1104 90-91 0.3339249561631874 1104 92-93 0.2564759256490632 1104 94-95 0.17834553633408134 1104 96-97 0.15623547924620595 1104 98-99 -0.10160728886215509 1104 100-101 0.1476475741209029 1105 1 -0.07227111900239436 1105 2 0.041500708014474696 1105 3 0.034754922638569496 1105 4 0.039346549674618814 1105 5 0.07708701660014583 1105 6 0.027291208794082422 1105 7 -0.010818489548860555 1105 8 -0.024451924793403634 1105 9 0.07621685318731153 1105 10-11 0.16928851082354868 1105 12-13 0.0576421015616333 1105 14-15 0.0651493443791864 1105 16-17 0.11306757244370402 1105 18-19 0.048904378549167404 1105 20-21 0.11401121986016705 1105 22-23 0.05375760734213486 1105 24-25 0.12059931809282887 1105 26-27 0.207162754338043 1105 28-29 0.08418279893364655 1105 30-31 0.1510256175272744 1105 32-33 0.16635727753565277 1105 34-35 0.038433829447079404 1105 36-37 0.03799990368745654 1105 38-39 0.12390045219623858 1105 40-41 0.17201277220127764 1105 42-43 0.24205146853739734 1105 44-45 0.28773484445635233 1105 46-47 0.06628559885762542 1105 48-49 0.1466229701873445 1105 50-51 -0.056002566196099224 1105 52-53 0.16824989940349866 1105 54-55 0.12602749815794567 1105 56-57 0.18658229186873854 1105 58-59 0.09155868661211031 1105 60-61 0.00262237368106355 1105 62-63 0.18625640848198088 1105 64-65 -0.0810306199496722 1105 66-67 -0.060528274820761396 1105 68-69 0.008168576214210077 1105 70-71 -0.06706615011176353 1105 72-73 -0.04982493108484931 1105 74-75 -0.10304531922057691 1105 76-77 0.08317414285607327 1105 78-79 -0.0014718730650358225 1105 80-81 0.14929534553783697 1105 82-83 0.12750341841268664 1105 84-85 0.18606027746878695 1105 86-87 0.25921634391521664 1105 88-89 0.2952198160664743 1105 90-91 0.37749461226443515 1105 92-93 0.4800839105942707 1105 94-95 0.36684930464521415 1105 96-97 0.2245067146249795 1105 98-99 0.11252341664686583 1105 100-101 0.5281230408933482 1106 1 0.04075120209919447 1106 2 -0.03036765425022736 1106 3 -0.02701950422731869 1106 4 0.05418303249890499 1106 5 -0.025170728950286048 1106 6 0.010085741131298676 1106 7 0.03779948818497303 1106 8 0.045873736233481566 1106 9 0.03131371834515306 1106 10-11 0.06601908285900038 1106 12-13 -0.05625914724814862 1106 14-15 -0.0018219714488409977 1106 16-17 0.02394567615717591 1106 18-19 0.05377395849497191 1106 20-21 0.13026566027619424 1106 22-23 -0.003284572032363542 1106 24-25 0.12476278033555843 1106 26-27 -0.01925154105490634 1106 28-29 -0.010391195792792018 1106 30-31 0.04856187329524175 1106 32-33 0.20169291805422063 1106 34-35 0.15532320357731066 1106 36-37 0.2315768647949099 1106 38-39 0.2407131490394221 1106 40-41 0.05280934621431754 1106 42-43 0.17891859481001404 1106 44-45 -0.009987039012479215 1106 46-47 -0.03046854858249759 1106 48-49 0.126627364825886 1106 50-51 -5.831986578357373E-4 1106 52-53 0.06605166458331269 1106 54-55 0.15890946549113494 1106 56-57 0.29199525055913966 1106 58-59 0.22329942124918745 1106 60-61 0.23088836833425574 1106 62-63 0.11186914011604898 1106 64-65 0.28304000555387177 1106 66-67 0.09751048039786525 1106 68-69 0.09938937017890481 1106 70-71 0.10699014703904197 1106 72-73 -0.06103812598706071 1106 74-75 0.1468539703128542 1106 76-77 0.11083404361048821 1106 78-79 0.0284482910014745 1106 80-81 0.11420690343719997 1106 82-83 0.09219867769636636 1106 84-85 0.17220114394502062 1106 86-87 9.603745311963507E-4 1106 88-89 0.07623062870838737 1106 90-91 0.19266115075414803 1106 92-93 0.1045437362736088 1106 94-95 0.025329812592186585 1106 96-97 -0.010109761607353107 1106 98-99 0.01142613033616513 1106 100-101 0.25336543267537337 1107 1 -0.17117500982701728 1107 2 -0.06874174262431154 1107 3 0.002324125116309972 1107 4 -0.004289725973848135 1107 5 -0.0015328053123582208 1107 6 0.07188325292880648 1107 7 0.07237692276240892 1107 8 0.048125988485729465 1107 9 0.022176009207449 1107 10-11 -0.01956650272659033 1107 12-13 0.05118401019500851 1107 14-15 -0.015378385005256234 1107 16-17 0.005723882935377844 1107 18-19 -0.08511493039392093 1107 20-21 -0.03836368335315399 1107 22-23 -0.13691820566599233 1107 24-25 0.005028760601533122 1107 26-27 0.04066909386573059 1107 28-29 -0.0811119165135068 1107 30-31 -0.18655181181843972 1107 32-33 -0.09576525440395045 1107 34-35 0.005409003663110923 1107 36-37 0.02586043407847427 1107 38-39 0.001245109571385683 1107 40-41 -0.0339988905939137 1107 42-43 -0.2451376042461817 1107 44-45 -0.286177515202958 1107 46-47 0.015713347599394467 1107 48-49 -0.21683037863186172 1107 50-51 -0.0488028468774786 1107 52-53 -0.12069222216057085 1107 54-55 -0.15189062030895428 1107 56-57 -0.15243846887457835 1107 58-59 -0.2468099738602021 1107 60-61 -0.044862382416503976 1107 62-63 -0.14730074405382965 1107 64-65 -0.04979997728611352 1107 66-67 -0.09109955821216431 1107 68-69 -0.02684400605447479 1107 70-71 0.05563879568769181 1107 72-73 -0.10773482268376 1107 74-75 -0.14824469978581334 1107 76-77 -0.2976923399158977 1107 78-79 -0.3873603748071943 1107 80-81 -0.08600759677729997 1107 82-83 -0.025379614881927637 1107 84-85 -0.42029134854746886 1107 86-87 -0.2422185186476966 1107 88-89 -0.018031490553731544 1107 90-91 -0.23439805130505675 1107 92-93 -0.13843138170151192 1107 94-95 -0.1299361676737938 1107 96-97 -0.4493633008608917 1107 98-99 -0.6054764865664488 1107 100-101 -0.23382890451896543 1108 1 -0.1513042362387722 1108 2 -0.1689099139820769 1108 3 -0.043734174287713756 1108 4 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60-61 -0.39846005541098606 2316 62-63 -0.44722225378857416 2316 64-65 -0.39052524677745737 2316 66-67 -0.3262984326180245 2316 68-69 -0.3134646963800023 2316 70-71 0.0074163693421809285 2316 72-73 -0.27545102326891424 2316 74-75 -0.29510168712045015 2316 76-77 -0.17799857345237768 2316 78-79 -0.25854399828720176 2316 80-81 -0.2675857128165262 2316 82-83 -0.38845857171471465 2316 84-85 -0.24491882354236694 2316 86-87 -0.24020255815041125 2316 88-89 -0.15564193583410812 2316 90-91 -0.14956028072019478 2316 92-93 -0.15146732684094744 2316 94-95 -0.1775374760068864 2316 96-97 0.05903102645566349 2316 98-99 -0.3066703648117688 2316 100-101 -0.46675326225960845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 12.0 5 31.0 6 42.0 7 134.0 8 290.0 9 442.0 10 788.0 11 1280.0 12 1832.0 13 2716.0 14 3400.0 15 4671.0 16 5931.0 17 8105.0 18 7688.0 19 5496.0 20 5000.0 21 5380.0 22 5758.0 23 6354.0 24 7477.0 25 9041.0 26 11000.0 27 13903.0 28 17272.0 29 21514.0 30 26560.0 31 32635.0 32 41220.0 33 53365.0 34 73339.0 35 110620.0 36 187980.0 37 335429.0 38 325093.0 39 55121.0 40 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.15105005216101 17.617987633176863 13.118332119978282 24.11263019468384 2 16.254179974918348 18.819316189636908 39.502088094436246 25.4244157410085 3 20.726018448955344 22.557863878029824 27.888507399625944 28.82761027338889 4 13.455163155154937 14.673942040228932 34.67110129909832 37.19979350551782 5 14.675960841032415 36.63734118166179 33.15216999456366 15.534527982742139 6 35.6818716013849 33.78448724622592 15.867990615460265 14.665650536928915 7 31.310807361701332 30.78000695044277 18.895903420569564 19.013282267286343 8 27.926936714920814 35.21963831741605 17.88635881877081 18.96706614889233 9 26.927197717024693 15.89510022624989 18.699286642316086 38.47841541440933 10-11 26.794481574183994 25.48340661745329 25.655654641630022 22.066457166732697 12-13 26.964050552232344 22.1864125283939 26.854458469106753 23.995078450267005 14-15 23.502230053887562 23.832952885515247 24.576412331412108 28.088404729185086 16-17 23.932110147968295 27.03805645831418 25.168237556801692 23.86159583691583 18-19 23.88681160695102 25.805537570603953 27.023703605434036 23.28394721701099 20-21 24.315418993245306 24.91316449663527 27.29769355530056 23.47372295481886 22-23 23.886657190309315 24.749695467174444 27.34861591227724 24.015031430239002 24-25 23.857007923975974 24.98485864463239 27.38703467370379 23.77109875768784 26-27 23.630655775410748 25.28258598350449 27.292178441052606 23.794579800032157 28-29 23.55043152862798 25.55575984368353 27.27491654229125 23.618892085397245 30-31 23.666394966492916 25.30193147459651 27.170137543256022 23.861536015654544 32-33 23.571262223843696 25.23755516636973 27.19899404516847 23.992188564618104 34-35 23.878100904585224 25.371971557406482 27.223105064840862 23.52682247316743 36-37 23.967841910422106 25.143614399977505 27.15481082554262 23.73373286405777 38-39 23.734237564804477 25.374406841434922 27.127268584793875 23.76408700896673 40-41 23.86953386928152 25.319910595190887 27.08071668048596 23.72983885504164 42-43 23.77956145328535 25.34449155994777 26.963174679626995 23.912772307139885 44-45 23.813173600883985 25.385598482950318 26.98553070601574 23.815697210149956 46-47 24.106527982993367 25.648114696705182 26.590063262888197 23.655294057413254 48-49 24.09403888456822 25.656798597868175 26.48460009424945 23.764562423314157 50-51 23.888638324397736 25.874767506796786 26.474103348659227 23.76249082014625 52-53 24.19088685268875 25.703783955279707 26.295379612320797 23.809949579710747 54-55 24.049274868065247 25.69550767526981 26.43057144268251 23.824646013982434 56-57 23.851748451416924 25.941785822182066 26.35666404453632 23.849801681864687 58-59 23.975179865581637 25.674966842437495 26.39269873898518 23.95715455299568 60-61 23.857251317828226 25.8391308642545 26.48140764459339 23.82221017332388 62-63 23.823399631713055 25.863866493818865 26.56759882390433 23.74513505056375 64-65 23.711803286134995 25.836071058153927 26.542225612558795 23.909900043152287 66-67 23.738141347780257 25.917400765125503 26.37977824915174 23.964679637942496 68-69 23.883242655530577 25.702037979411113 26.611904291537908 23.8028150735204 70-71 23.832012216664094 25.728500790939595 26.401880950973283 24.03760604142303 72-73 23.882026662282918 25.954502013604763 26.286816197234124 23.87665512687819 74-75 23.605657545051702 26.278838208977607 26.174076867282594 23.941427378688097 76-77 23.913242142729946 26.02818795970507 26.109965479339436 23.94860441822555 78-79 23.925299951750777 26.03577948841387 26.109614875917934 23.929305683917416 80-81 23.947981944443946 26.134880238279695 26.03026155032494 23.88687626695142 82-83 23.951305082619424 26.19704072642221 26.082690080910652 23.768964110047715 84-85 23.899115543208747 26.26238956907696 25.933142651553442 23.90535223616085 86-87 23.84230016819583 26.247067401049602 26.01917288076865 23.891459549985925 88-89 23.83354455739946 26.263545989734325 25.85422457197664 24.04868488088957 90-91 23.76590544264747 26.371311602569968 25.932918602393134 23.92986435238943 92-93 23.89846582258527 26.23247055965135 26.005270718156137 23.863792899607244 94-95 23.790409946020933 26.061078218636673 26.1203099082756 24.028201927066792 96-97 23.860034933584732 25.989793913480796 26.07592016107663 24.07425099185784 98-99 23.63422088817011 26.106030705470186 25.95443569523527 24.30531271112444 100-101 23.720745621419077 26.129014698073217 25.862819184967307 24.287420495540395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 106.0 1 78.5 2 133.0 3 218.5 4 167.0 5 151.5 6 354.5 7 702.0 8 1118.0 9 1258.0 10 1018.5 11 846.0 12 857.0 13 912.0 14 998.0 15 1092.5 16 1152.5 17 1234.0 18 1314.5 19 1411.5 20 1541.0 21 1761.0 22 2116.5 23 2614.5 24 3225.0 25 3880.0 26 4962.0 27 6269.0 28 7405.0 29 8668.5 30 10148.0 31 12075.5 32 14707.0 33 16372.0 34 17548.0 35 19557.5 36 22026.0 37 24436.5 38 26885.0 39 29092.5 40 31030.5 41 33971.5 42 36283.5 43 38701.5 44 41713.5 45 44554.5 46 47636.0 47 50181.0 48 52593.0 49 54055.0 50 54135.5 51 54556.5 52 55791.5 53 57548.5 54 58864.0 55 57050.5 56 53354.5 57 49921.0 58 47101.0 59 43220.5 60 37148.5 61 30951.5 62 25495.0 63 19833.0 64 14650.5 65 11106.5 66 8217.5 67 6298.0 68 4898.0 69 3816.0 70 3041.0 71 2208.0 72 1663.5 73 1399.0 74 1102.5 75 801.5 76 615.5 77 415.5 78 269.0 79 170.0 80 91.5 81 63.5 82 44.0 83 20.5 84 9.5 85 3.5 86 2.5 87 2.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2700146074658138 2 0.020981108350481125 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.128502439143971E-4 12-13 3.6050014347905705E-5 14-15 0.0 16-17 6.849502726102084E-4 18-19 0.0 20-21 3.6050014347905705E-5 22-23 1.0815004304371714E-4 24-25 0.002307200918265965 26-27 0.0028119011191366453 28-29 0.002307200918265965 30-31 0.0011896504734808884 32-33 0.00151410060261204 34-35 0.00201880080348272 36-37 1.0815004304371714E-4 38-39 1.0815004304371714E-4 40-41 8.652003443497371E-4 42-43 0.0045423018078361195 44-45 0.00428995170740078 46-47 0.015068905997424586 48-49 0.015609656212643173 50-51 0.006741352683058368 52-53 0.008615953429149465 54-55 5.407502152185857E-4 56-57 0.0035329014060947596 58-59 0.0016943506743515684 60-61 0.00151410060261204 62-63 0.0 64-65 9.733503873934541E-4 66-67 0.0 68-69 0.0 70-71 2.8840011478324564E-4 72-73 0.0015501506169599455 74-75 0.0 76-77 0.19600392800956334 78-79 0.10436479153718703 80-81 0.001478050588264134 82-83 0.0 84-85 6.489002582623028E-4 86-87 0.12055124797939669 88-89 0.5836136822782455 90-91 0.3099219733489454 92-93 0.2911759658880344 94-95 0.0025595510187013054 96-97 0.0032084512769636085 98-99 0.003100301233919891 100-101 8.291503300018313E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1386962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.03383118369708 #Duplication Level Percentage of deduplicated Percentage of total 1 77.98764702522695 47.59884882951458 2 12.90223610163669 15.749458002389915 3 4.0012817577354785 7.326406659601019 4 1.762123696662524 4.3019664090757095 5 0.924134231199988 2.820172632906788 6 0.5473903412041119 2.004559780598287 7 0.35618476781027714 1.5217524692115758 8 0.26425588983673576 1.2902839495674387 9 0.19209995734027233 1.0552136730031434 >10 0.983357102604885 10.572904876047813 >50 0.056873192322641465 2.3691066657659454 >100 0.02088224466122213 2.312124533468192 >500 0.001179762890916111 0.480996400128042 >1k 3.539288672748334E-4 0.5962051187215851 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3683 0.2655444056866735 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2710 0.19539107776564896 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1887 0.13605275414899615 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.210002869581142E-5 7 0.0 0.0 0.0 0.0 7.210002869581142E-5 8 0.0 0.0 0.0 0.0 7.210002869581142E-5 9 0.0 0.0 0.0 0.0 7.210002869581142E-5 10-11 0.0 0.0 0.0 0.0 1.0815004304371714E-4 12-13 0.0 0.0 0.0 0.0 3.605001434790571E-4 14-15 0.0 0.0 0.0 0.0 3.605001434790571E-4 16-17 0.0 0.0 0.0 0.0 5.768002295664914E-4 18-19 0.0 0.0 0.0 0.0 5.768002295664914E-4 20-21 0.0 0.0 0.0 7.210002869581142E-5 6.489002582623028E-4 22-23 0.0 0.0 0.0 3.244501291311514E-4 6.489002582623028E-4 24-25 1.4420005739162285E-4 0.0 0.0 3.9655015782696283E-4 6.489002582623028E-4 26-27 1.4420005739162285E-4 0.0 0.0 5.768002295664914E-4 7.210002869581142E-4 28-29 2.1630008608743427E-4 0.0 0.0 8.291503300018314E-4 7.210002869581142E-4 30-31 2.1630008608743427E-4 0.0 0.0 0.0024874509900054942 7.210002869581142E-4 32-33 2.1630008608743427E-4 0.0 0.0 0.004794651908271459 7.210002869581142E-4 34-35 2.1630008608743427E-4 0.0 0.0 0.007606553027408105 7.931003156539257E-4 36-37 2.1630008608743427E-4 0.0 0.0 0.012797755093506526 7.931003156539257E-4 38-39 2.884001147832457E-4 0.0 0.0 0.02058455819265416 8.291503300018314E-4 40-41 2.884001147832457E-4 0.0 0.0 0.033021813142681634 8.652003443497371E-4 42-43 2.884001147832457E-4 0.0 0.0 0.04394496749009706 8.652003443497371E-4 44-45 2.884001147832457E-4 0.0 0.0 0.05631012241142872 8.652003443497371E-4 46-47 2.884001147832457E-4 0.0 0.0 0.0720279286671156 9.012503586976428E-4 48-49 2.884001147832457E-4 0.0 0.0 0.08825043512367317 0.0010815004304371714 50-51 2.884001147832457E-4 0.0 0.0 0.1066359424411051 0.0011536004591329828 52-53 2.884001147832457E-4 0.0 0.0 0.1249493497298412 0.0012257004878287942 54-55 2.884001147832457E-4 0.0 0.0 0.1451734077790163 0.0012257004878287942 56-57 2.884001147832457E-4 0.0 0.0 0.17199461845385816 0.0012257004878287942 58-59 2.884001147832457E-4 0.0 0.0 0.20234873053479474 0.0012617505021766998 60-61 2.884001147832457E-4 0.0 0.0 0.23742539449530703 0.0012978005165246056 62-63 2.884001147832457E-4 0.0 0.0 0.2713484579966863 0.0012978005165246056 64-65 2.884001147832457E-4 0.0 0.0 0.30819157266024594 0.001369900545220417 66-67 2.884001147832457E-4 0.0 0.0 0.34943278907425 0.001369900545220417 68-69 2.884001147832457E-4 0.0 0.0 0.39770375828609583 0.001369900545220417 70-71 2.884001147832457E-4 0.0 0.0 0.45480698101317846 0.001369900545220417 72-73 2.884001147832457E-4 0.0 0.0 0.516128055418966 0.0014420005739162285 74-75 2.884001147832457E-4 0.0 0.0 0.5775572798677974 0.0015501506169599455 76-77 2.884001147832457E-4 0.0 0.0 0.6416181553640259 0.0015862006313078513 78-79 2.884001147832457E-4 3.605001434790571E-5 0.0 0.7212886870728974 0.0015862006313078513 80-81 2.884001147832457E-4 7.210002869581142E-5 0.0 0.8103322225122246 0.0016583006600036628 82-83 2.884001147832457E-4 7.210002869581142E-5 0.0 0.9056124104337393 0.0018025007173952856 84-85 4.6865018652277426E-4 7.210002869581142E-5 0.0 0.9986935474800318 0.0018025007173952856 86-87 5.0470020087068E-4 7.210002869581142E-5 0.0 1.0996696376685158 0.0019106507604390028 88-89 5.0470020087068E-4 7.210002869581142E-5 0.0 1.2185986350022566 0.00201880080348272 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2595 0.0 42.899364 1 GTATCAA 4200 0.0 34.548004 1 ATCAACG 5315 0.0 27.04316 3 TCAACGC 5345 0.0 26.891375 4 TATCAAC 5445 0.0 26.57845 2 CAACGCA 5425 0.0 26.49482 5 GTACATG 5260 0.0 26.31962 1 TACATGG 5160 0.0 26.023422 2 AACGCAG 5620 0.0 25.828737 6 TAACGCA 115 1.2935257E-4 24.749786 4 GTATAGG 405 0.0 24.668154 1 ACATGGG 5140 0.0 24.456867 3 CCTATAC 215 1.6880222E-9 24.27014 3 GATAGCG 185 2.5751433E-7 23.077503 7 ACGCAGA 6520 0.0 22.117907 7 CGCAGAG 6645 0.0 21.701845 8 GAGTACA 3715 0.0 21.642143 1 GTACCTG 1340 0.0 21.301926 1 AGAGTAC 5935 0.0 20.30237 10-11 GTAGGAC 305 2.1282176E-10 20.219088 3 >>END_MODULE