##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936281_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54228 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.087408718743085 31.0 31.0 34.0 30.0 34.0 2 31.048701777679426 33.0 31.0 34.0 28.0 34.0 3 31.092535221656707 33.0 31.0 34.0 28.0 34.0 4 34.538946669617175 37.0 35.0 37.0 32.0 37.0 5 34.63625802168622 37.0 35.0 37.0 32.0 37.0 6 34.89099727078262 37.0 35.0 37.0 33.0 37.0 7 34.860570185144205 37.0 35.0 37.0 33.0 37.0 8 34.91299697573209 37.0 35.0 37.0 33.0 37.0 9 36.381666297853506 39.0 37.0 39.0 32.0 39.0 10-11 36.41868591871358 39.0 37.0 39.0 32.5 39.0 12-13 36.33577672051339 39.0 37.0 39.0 32.0 39.0 14-15 37.1057848344029 40.0 37.0 41.0 32.0 41.0 16-17 37.16721066607656 40.0 37.5 41.0 32.5 41.0 18-19 37.21464003835657 40.0 37.0 41.0 33.0 41.0 20-21 37.179888618425906 40.0 37.0 41.0 33.0 41.0 22-23 36.97389724865383 40.0 35.5 41.0 32.5 41.0 24-25 36.893864793095815 40.0 35.5 41.0 32.5 41.0 26-27 36.572010769344246 39.5 35.0 41.0 32.5 41.0 28-29 36.3210979567751 39.0 35.0 41.0 32.0 41.0 30-31 35.85881832263775 38.5 35.0 41.0 31.0 41.0 32-33 35.12936121560817 37.0 35.0 40.0 30.0 41.0 34-35 34.634653684443464 37.0 35.0 40.0 29.0 41.0 36-37 34.159032234270114 36.0 35.0 40.0 25.0 41.0 38-39 33.77734749575865 35.5 35.0 40.0 22.5 41.0 40-41 33.44089769122962 35.0 35.0 40.0 13.5 41.0 42-43 32.875368813159255 35.0 34.0 40.0 2.0 41.0 44-45 32.21207678689976 35.0 33.0 40.0 2.0 41.0 46-47 31.32759828870694 35.0 33.0 40.0 2.0 41.0 48-49 30.5804842516781 35.0 32.5 40.0 2.0 41.0 50-51 29.879619384819648 35.0 30.5 39.5 2.0 40.5 52-53 29.781717931695802 35.0 31.0 39.5 2.0 40.5 54-55 29.620233089916645 35.0 30.0 40.0 2.0 41.0 56-57 29.080770081876523 35.0 27.0 40.0 2.0 41.0 58-59 28.614009367854244 35.0 24.0 40.0 2.0 41.0 60-61 27.908543556834108 35.0 19.0 39.0 2.0 41.0 62-63 27.544276019768386 35.0 5.0 39.0 2.0 41.0 64-65 27.072084531976103 35.0 2.0 38.5 2.0 40.5 66-67 26.442271520247843 35.0 2.0 37.0 2.0 39.5 68-69 25.83909603894667 35.0 2.0 37.0 2.0 39.0 70-71 25.101589584716383 34.0 2.0 36.0 2.0 38.0 72-73 24.492015195102162 33.5 2.0 35.0 2.0 37.0 74-75 24.089741461975365 33.5 2.0 35.0 2.0 37.0 76-77 23.68404698679649 33.0 2.0 35.0 2.0 36.5 78-79 23.194982296968355 33.0 2.0 35.0 2.0 36.0 80-81 22.726405178136755 32.0 2.0 35.0 2.0 35.5 82-83 22.260806225566128 31.5 2.0 35.0 2.0 35.0 84-85 21.887401342479897 31.0 2.0 35.0 2.0 35.0 86-87 21.72584273806889 31.5 2.0 35.0 2.0 35.0 88-89 21.251161761451648 31.0 2.0 35.0 2.0 35.0 90-91 20.754103046396693 30.0 2.0 35.0 2.0 35.0 92-93 20.33264180865973 29.0 2.0 35.0 2.0 35.0 94-95 19.834799365641366 29.0 2.0 34.5 2.0 35.0 96-97 19.363225639890832 29.0 2.0 34.0 2.0 35.0 98-99 18.99513166629785 28.0 2.0 34.0 2.0 35.0 100-101 16.861944014162425 14.5 2.0 32.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.073902350126616 1101 2 1.0965869653919889 1101 3 1.049473150165408 1101 4 1.2886903996381065 1101 5 1.6458659707487229 1101 6 1.363357537498338 1101 7 1.2982436588929218 1101 8 1.1033728597007695 1101 9 0.9512034899684991 1101 10-11 1.5833914557836906 1101 12-13 1.0846996425539857 1101 14-15 1.1036678123847636 1101 16-17 0.0979542110049465 1101 18-19 1.3684149465415274 1101 20-21 1.4434048718926746 1101 22-23 1.4692132783520293 1101 24-25 1.7020333534451453 1101 26-27 1.1131056582345238 1101 28-29 1.282665936708021 1101 30-31 1.1183020371777488 1101 32-33 0.6609246993928934 1101 34-35 0.6832823494432176 1101 36-37 0.6743136318257967 1101 38-39 0.7185771108021157 1101 40-41 0.8852174001931417 1101 42-43 1.155051970719299 1101 44-45 1.2486497969443846 1101 46-47 -0.2547122039227574 1101 48-49 0.6708193402607563 1101 50-51 1.78820490171805 1101 52-53 1.846362627080488 1101 54-55 0.769245811719113 1101 56-57 1.2167931086937251 1101 58-59 1.4755286150036504 1101 60-61 1.2095750104082548 1101 62-63 0.5101778469684142 1101 64-65 1.1690033419896437 1101 66-67 1.366667417295421 1101 68-69 1.9296408837090517 1101 70-71 2.4875691039041783 1101 72-73 2.5514581336243722 1101 74-75 2.650868242746146 1101 76-77 2.321858492826408 1101 78-79 1.224633323946371 1101 80-81 1.0510595902721143 1101 82-83 1.4176727247100764 1101 84-85 1.0640372980352417 1101 86-87 1.4995254113251804 1101 88-89 -0.2673317617034705 1101 90-91 0.3717263497509471 1101 92-93 0.7699083187900335 1101 94-95 0.10578268009640723 1101 96-97 0.009990187879637347 1101 98-99 0.11313423914680243 1101 100-101 0.4227219933238473 1102 1 -2.2488791755609796 1102 2 -2.208031612708684 1102 3 -2.3983732431921396 1102 4 -2.233364608146019 1102 5 -2.1097126488672586 1102 6 -2.2214893748005693 1102 7 -2.1220988424044265 1102 8 -2.1986510116017755 1102 9 -2.6393517772863007 1102 10-11 -2.7430226594212925 1102 12-13 -2.9965146802275413 1102 14-15 -3.746617605363028 1102 16-17 -2.9700271071061053 1102 18-19 -2.585918213811354 1102 20-21 -2.7210995391088844 1102 22-23 -2.4365988648757906 1102 24-25 -2.143841062745828 1102 26-27 -2.964216604349808 1102 28-29 -2.942814084049637 1102 30-31 -2.2690876877833333 1102 32-33 -1.961804932158742 1102 34-35 -2.4698053516465563 1102 36-37 -2.344368257120756 1102 38-39 -2.30711048649939 1102 40-41 -3.5268220393501934 1102 42-43 -3.887501220770062 1102 44-45 -3.3790097775236987 1102 46-47 -3.266128913834539 1102 48-49 -3.4285579301076936 1102 50-51 -3.881229452199957 1102 52-53 -4.0610063402262035 1102 54-55 -4.082336959427749 1102 56-57 -4.146984784404353 1102 58-59 -5.2701901187794356 1102 60-61 -5.040814195611468 1102 62-63 -4.340885982818818 1102 64-65 -5.707488614419283 1102 66-67 -5.146825058054862 1102 68-69 -5.8402086232966575 1102 70-71 -6.1457988255197975 1102 72-73 -6.101889038145217 1102 74-75 -6.154528747393972 1102 76-77 -5.6547891459904065 1102 78-79 -5.032761590428301 1102 80-81 -4.383553798414965 1102 82-83 -5.570651094698334 1102 84-85 -5.193098145659622 1102 86-87 -4.872815014206736 1102 88-89 -5.326231606021061 1102 90-91 -5.462990827208056 1102 92-93 -5.044829615823355 1102 94-95 -4.404596147096118 1102 96-97 -5.054099070034219 1102 98-99 -5.076798298476447 1102 100-101 -4.728549413007238 1103 1 0.6053336847494428 1103 2 0.8296624005950832 1103 3 0.738544716896552 1103 4 0.5681874982841393 1103 5 0.40747138660945836 1103 6 0.6476902260863469 1103 7 0.6933113571521119 1103 8 0.6217481014802644 1103 9 1.1078766040884176 1103 10-11 1.079039424835912 1103 12-13 0.8252702421671358 1103 14-15 1.5995575609340946 1103 16-17 1.2367356423395748 1103 18-19 1.0033279059225748 1103 20-21 1.1622153167669538 1103 22-23 1.6096871661663386 1103 24-25 0.9783873186288972 1103 26-27 1.7134441495304529 1103 28-29 1.5810218361277535 1103 30-31 1.0116772789572437 1103 32-33 1.0185165756017867 1103 34-35 1.5892301250718361 1103 36-37 2.0292459335666067 1103 38-39 1.587290843877554 1103 40-41 1.5114262976786321 1103 42-43 0.5102260519572113 1103 44-45 0.7118993133853948 1103 46-47 1.5354231925956192 1103 48-49 2.2665640211118188 1103 50-51 1.1581275322789786 1103 52-53 1.2929293582216879 1103 54-55 1.2247583842529615 1103 56-57 0.876851135773034 1103 58-59 1.2871823867638064 1103 60-61 1.519052766694518 1103 62-63 0.5341140171811816 1103 64-65 0.3288195895718502 1103 66-67 0.792199332189039 1103 68-69 1.5880064543086654 1103 70-71 0.595886318604375 1103 72-73 0.17887592859536028 1103 74-75 0.6847173723399536 1103 76-77 0.20773857019584696 1103 78-79 0.44561978429453575 1103 80-81 0.2860692614519955 1103 82-83 0.6787945815766108 1103 84-85 0.3590082844955873 1103 86-87 0.9453668039750838 1103 88-89 1.8472717199212916 1103 90-91 2.1362331195768647 1103 92-93 0.7312235992542497 1103 94-95 0.6123107265180714 1103 96-97 0.8153576927152244 1103 98-99 0.5019156704814236 1103 100-101 -1.0057112755349564 1104 1 1.8062427756585322 1104 2 1.571480582413269 1104 3 1.6830901714420072 1104 4 1.6027329528295908 1104 5 1.7956532047912788 1104 6 1.6531447715408945 1104 7 1.530584084424838 1104 8 1.867202646934814 1104 9 1.7533311495429658 1104 10-11 1.9972212430177265 1104 12-13 2.0629975148944126 1104 14-15 2.6172848336613583 1104 16-17 2.4058265514304864 1104 18-19 2.189691542286205 1104 20-21 1.9608516804033158 1104 22-23 2.4155962570754355 1104 24-25 2.2588418640834433 1104 26-27 2.174807785894096 1104 28-29 2.5801127452186634 1104 30-31 2.4521318244117865 1104 32-33 2.646243848329064 1104 34-35 2.9260483068900243 1104 36-37 2.831973206293881 1104 38-39 2.2741090256957293 1104 40-41 2.164153570405901 1104 42-43 2.4824987792299424 1104 44-45 2.780990222476298 1104 46-47 2.389968647141078 1104 48-49 1.5265640211118168 1104 50-51 1.3526729868244338 1104 52-53 1.2133839036762346 1104 54-55 1.5083947478893265 1104 56-57 2.225942044863938 1104 58-59 1.5712732958547129 1104 60-61 1.6272345848763372 1104 62-63 1.6691140171811796 1104 64-65 2.3583650441173027 1104 66-67 1.8121993321890386 1104 68-69 2.0602791815813895 1104 70-71 2.15224995496801 1104 72-73 2.259330474049907 1104 74-75 2.241081008703592 1104 76-77 1.8122840247412988 1104 78-79 2.2301652388399873 1104 80-81 2.794251079633817 1104 82-83 2.6078854906675204 1104 84-85 2.414462829950132 1104 86-87 2.267184985793264 1104 88-89 1.961817174466745 1104 90-91 2.2589603923041395 1104 92-93 0.6339508719815221 1104 94-95 0.8600379992453462 1104 96-97 0.8512667836243182 1104 98-99 0.9110065795723337 1104 100-101 0.7897432699195903 1105 1 0.6129094423252006 1105 2 0.7823139157465988 1105 3 0.6097568381086731 1105 4 0.6268996194962568 1105 5 0.3923198714579428 1105 6 0.2764781048742293 1105 7 0.5664174177581742 1105 8 0.8547026469348111 1105 9 0.19499781620962864 1105 10-11 0.7097212430177322 1105 12-13 0.5042475148944092 1105 14-15 0.04936816699468949 1105 16-17 0.3333265514304813 1105 18-19 0.8376082089528794 1105 20-21 1.192518347069985 1105 22-23 0.8360129237420963 1105 24-25 1.2217585307501082 1105 26-27 0.8393911192274217 1105 28-29 1.0734460785519886 1105 30-31 1.3175484910784547 1105 32-33 0.062077181662388625 1105 34-35 -0.6872850264433126 1105 36-37 0.16655653962720862 1105 38-39 -0.4288076409709305 1105 40-41 -0.4554297629274231 1105 42-43 0.10208211256326649 1105 44-45 -0.5248431108570308 1105 46-47 -0.26003135285892753 1105 48-49 1.5998973544451474 1105 50-51 1.7689229868244318 1105 52-53 1.598800570342899 1105 54-55 1.067561414555989 1105 56-57 0.9421920448639405 1105 58-59 1.1441899625213772 1105 60-61 1.6705679182096702 1105 62-63 1.6070306838478459 1105 64-65 0.6062817107839713 1105 66-67 0.02136599885570689 1105 68-69 0.8986125149147242 1105 70-71 0.5759999549680082 1105 72-73 0.630580474049907 1105 74-75 0.12316434203692594 1105 76-77 1.1310340247413002 1105 78-79 0.9209985721733212 1105 80-81 -0.10408225369952007 1105 82-83 -0.7766978426658149 1105 84-85 0.06071282995013405 1105 86-87 -0.03948168087340065 1105 88-89 -0.12526615886658732 1105 90-91 0.565210392304138 1105 92-93 0.6289508719815231 1105 94-95 -0.16704533408799094 1105 96-97 -0.6041498830423464 1105 98-99 0.23392324623899796 1105 100-101 0.6439099365862546 1106 1 -1.9028138281150504 1106 2 -1.328142059096166 1106 3 -2.0569098285579948 1106 4 -1.7602859150949328 1106 5 -1.9394411348313625 1106 6 -1.6181759831760871 1106 7 -1.3199819533110073 1106 8 -2.6252501832538684 1106 9 -2.45987639762685 1106 10-11 -2.041458002265287 1106 12-13 -1.9279458813320076 1106 14-15 -1.5215830908669403 1106 16-17 -1.7739847693242368 1106 18-19 -2.0631386463930355 1106 20-21 -1.8538653007287635 1106 22-23 -1.688554686320792 1106 24-25 -1.5398373811995683 1106 26-27 -1.4874563650493045 1106 28-29 -1.75988725478134 1106 30-31 -1.4782455340787735 1106 32-33 -0.9988504912935809 1106 34-35 -0.7265932025439383 1106 36-37 -0.7734984918193248 1106 38-39 -0.9690985214740735 1106 40-41 -1.2960351088393764 1106 42-43 -1.217689900015344 1106 44-45 -0.4922173246935131 1106 46-47 -5.973905947769254E-4 1106 48-49 0.8986394928099308 1106 50-51 1.2588994019187716 1106 52-53 1.123761262166802 1106 54-55 1.0317909743044211 1106 56-57 1.8095269505243188 1106 58-59 2.307122352458485 1106 60-61 2.7994987358197356 1106 62-63 2.177981941709483 1106 64-65 1.3753461761927745 1106 66-67 1.792199332189039 1106 68-69 1.5629206910153535 1106 70-71 2.307721653081213 1106 72-73 2.009707832540471 1106 74-75 2.547496103043212 1106 76-77 3.0207745907790375 1106 78-79 2.36714637091546 1106 80-81 1.6231190041621169 1106 82-83 1.7954326604788413 1106 84-85 2.149745848818057 1106 86-87 2.561147249944206 1106 88-89 2.1178549103158026 1106 90-91 1.0551868073984778 1106 92-93 1.5322527587739785 1106 94-95 1.1232455464151556 1106 96-97 1.201078104379036 1106 98-99 1.1091197871195035 1106 100-101 0.7755923265233626 1107 1 0.3375471234846188 1107 2 0.250611017195876 1107 3 0.6170032149202669 1107 4 0.5227329528295925 1107 5 0.6060879873999738 1107 6 0.8235795541495889 1107 7 0.5401493018161432 1107 8 0.6672026469348111 1107 9 1.0446354973690504 1107 10-11 0.9515690691046856 1107 12-13 0.7012583844596278 1107 14-15 0.5190239640961423 1107 16-17 0.4501743775174347 1107 18-19 0.7483871944601219 1107 20-21 0.6943299412728763 1107 22-23 0.5212484309884786 1107 24-25 0.464059255387788 1107 26-27 -0.23171395323634414 1107 28-29 0.4248953539143159 1107 30-31 0.627783998324837 1107 32-33 1.1227655874594973 1107 34-35 -0.09308212789258619 1107 36-37 0.2584949454243173 1107 38-39 0.5571525039566012 1107 40-41 1.0232840051885148 1107 42-43 0.427716170534282 1107 44-45 0.2079467442154268 1107 46-47 2.6312729949671585 1107 48-49 2.1252596732857327 1107 50-51 1.1778903781287795 1107 52-53 0.10468825150232064 1107 54-55 -0.8029095999367613 1107 56-57 -0.8118840420925828 1107 58-59 -1.037422356319201 1107 60-61 -0.8149393281671387 1107 62-63 -0.7974077219492521 1107 64-65 -0.026417564578348873 1107 66-67 -0.3382354504196563 1107 68-69 0.10332265984225941 1107 70-71 -0.21340221894503486 1107 72-73 -0.509365178124007 1107 74-75 0.09282013913837162 1107 76-77 -0.022498583954352114 1107 78-79 0.23712176057911805 1107 80-81 0.9194684709381633 1107 82-83 0.8161463602327395 1107 84-85 0.7414193516892666 1107 86-87 0.6380545510106543 1107 88-89 -0.0894871733593412 1107 90-91 0.39809082708674737 1107 92-93 -0.5069186932358676 1107 94-95 -0.9356141746677018 1107 96-97 0.6073537401460598 1107 98-99 -0.7846455943407129 1107 100-101 -2.503734990949976 1108 1 0.39068722010297563 1108 2 -0.06259349166080597 1108 3 -0.44579871744688404 1108 4 0.44865887875551635 1108 5 -0.005828276690202472 1108 6 -0.23278115438503022 1108 7 0.2076211214618766 1108 8 -0.24020476047259365 1108 9 -0.05037255416073805 1108 10-11 0.02685087264736552 1108 12-13 -0.02700248510559078 1108 14-15 0.07135890773543707 1108 16-17 0.062493218097145586 1108 18-19 -0.3880862354915706 1108 20-21 -0.5343335047818769 1108 22-23 -1.5355148540356822 1108 24-25 -1.0363433211017394 1108 26-27 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2311 44-45 1.2258289321537248 2311 46-47 2.344807356818489 2311 48-49 2.879467246918267 2311 50-51 3.3062213739212005 2311 52-53 2.8988677746439784 2311 54-55 2.7496850704699654 2311 56-57 2.8020710771220045 2311 58-59 1.5228861990805207 2311 60-61 1.2565894235860178 2311 62-63 1.0784688558908613 2311 64-65 1.3277198828269832 2311 66-67 1.3163928805761351 2311 68-69 1.190279181581392 2311 70-71 1.5064435033551042 2311 72-73 0.5348143450176508 2311 74-75 1.3452745570906863 2311 76-77 1.7590582182896846 2311 78-79 2.0775845936787007 2311 80-81 1.7010252731822 2311 82-83 2.104014522925585 2311 84-85 2.194785410595294 2311 86-87 2.3368624051481035 2311 88-89 1.834075238982873 2311 90-91 1.134766843917042 2311 92-93 0.9071766784331352 2311 94-95 0.45358638634212056 2311 96-97 1.700621622333994 2311 98-99 2.3464904505400774 2311 100-101 1.9026464957260423 2312 1 -0.4160760649211781 2312 2 0.025973336036454242 2312 3 -0.3902431618913269 2312 4 -0.1149482065906966 2312 5 -0.2779699836145184 2312 6 0.01923172806263551 2312 7 0.09812031630889351 2312 8 0.38459395128263907 2312 9 0.6533311495429643 2312 10-11 0.19069950388729495 2312 12-13 0.36067867431469836 2312 14-15 -0.20561371706327947 2312 16-17 -0.4519995355260349 2312 18-19 -0.5016128055398781 2312 20-21 -0.5919019427850927 2312 22-23 -0.6055631632144269 2312 24-25 -0.14101320838032194 2312 26-27 -0.20635163439575877 2312 28-29 -0.9048147910132229 2312 30-31 -0.7164189002258894 2312 32-33 -0.4823068763086198 2312 34-35 -0.6619227076027272 2312 36-37 -0.980635489358292 2312 38-39 -1.471833003289774 2312 40-41 -1.8209188933622116 2312 42-43 -1.6990954236686129 2312 44-45 -1.6108938354947142 2312 46-47 -1.2926400485110996 2312 48-49 -1.3421316310620952 2312 50-51 -1.6300806363639744 2312 52-53 -0.9279204441498514 2312 54-55 -0.6797211941396597 2312 56-57 -0.9785507087592471 2312 58-59 -1.9866977186380375 2312 60-61 -2.43812773396424 2312 62-63 -2.1742193161521506 2312 64-65 -1.435837854433423 2312 66-67 -2.2947571895500936 2312 68-69 -2.7118947314620847 2312 70-71 -3.3329674363363395 2312 72-73 -2.679655033196468 2312 74-75 -1.78036826665873 2312 76-77 -2.047860902794934 2312 78-79 -2.92954490608755 2312 80-81 -3.4174880508009657 2312 82-83 -2.9266072629556668 2312 84-85 -2.3636531120788504 2312 86-87 -3.039481680873404 2312 88-89 -2.346733550170935 2312 90-91 -3.0729236656668775 2312 92-93 -3.6554694178735474 2312 94-95 -3.714599681914077 2312 96-97 -3.58467524536119 2312 98-99 -2.6976890726015803 2312 100-101 -2.9964886141383804 2313 1 0.2795761089918649 2313 2 0.551163122095808 2313 3 -0.05690982855799476 2313 4 0.6973361274327701 2313 5 0.8724786016166775 2313 6 0.7169542953504191 2313 7 0.7102666241073763 2313 8 0.5640280437602101 2313 9 0.288251784463597 2313 10-11 0.5996021953986812 2313 12-13 0.611886403783295 2313 14-15 -0.05033421395769011 2313 16-17 0.4672551228590507 2313 18-19 0.6277867803814488 2313 20-21 0.12307390262554208 2313 22-23 -0.05138786990869448 2313 24-25 0.10915932440089904 2313 26-27 0.2367125477988523 2313 28-29 0.287731792837711 2313 30-31 0.04721118949115777 2313 32-33 -0.11550218341697871 2313 34-35 0.3008102116519282 2313 36-37 0.07975098407165859 2313 38-39 0.0964899780766828 2313 40-41 0.21669325294558917 2313 42-43 0.5807527474839063 2313 44-45 0.557498158984238 2313 46-47 1.0455242026966296 2313 48-49 2.3062465607943636 2313 50-51 1.4975936217450645 2313 52-53 1.9157648560571872 2313 54-55 2.0010931605877396 2313 56-57 1.742291251213146 2313 58-59 1.6188923434737603 2313 60-61 2.1467583944001483 2313 62-63 1.9210187790859443 2313 64-65 1.7456666314188922 2313 66-67 1.744580284569988 2313 68-69 1.360914102216313 2313 70-71 0.8195515422695969 2313 72-73 0.7937749184943499 2313 74-75 0.908382596005179 2313 76-77 0.5114903739476482 2313 78-79 0.4438160324907834 2313 80-81 0.4693304447131794 2313 82-83 1.0085204113024382 2313 84-85 0.5473199728072764 2313 86-87 0.20655006515834273 2313 88-89 0.1629282855778591 2313 90-91 0.4367381700819166 2313 92-93 0.5436334116640609 2313 94-95 1.4624189516262973 2313 96-97 1.285235037592571 2313 98-99 0.8157684843342388 2313 100-101 1.476251206427527 2314 1 0.581163410579169 2314 2 0.725766296698982 2314 3 0.48277271112454656 2314 4 0.8084472385438772 2314 5 0.23755796669603768 2314 6 0.49473207312819767 2314 7 0.6150285288692814 2314 8 0.8338693136014754 2314 9 0.31999781620962864 2314 10-11 0.3297609255574159 2314 12-13 0.6674419593388521 2314 14-15 0.1877610241375507 2314 16-17 0.8482075038114303 2314 18-19 0.5881042406989039 2314 20-21 0.4405342200858513 2314 22-23 0.6073422888214637 2314 24-25 0.5853498005913806 2314 26-27 0.29226810335440945 2314 28-29 -0.12496661986070734 2314 30-31 -0.5162808740009055 2314 32-33 0.4321168642020723 2314 34-35 0.07065148149320066 2314 36-37 0.7146716189922984 2314 38-39 0.5806169622036705 2314 40-41 0.9151059513582851 2314 42-43 1.017260683991843 2314 44-45 1.255910857396934 2314 46-47 1.0058416630140847 2314 48-49 0.8697386242864233 2314 50-51 -0.010342886191441636 2314 52-53 0.6141775544698866 2314 54-55 1.1677598272544039 2314 56-57 1.1470531559750512 2314 58-59 1.983971708553124 2314 60-61 2.876917124558876 2314 62-63 1.9686378267049918 2314 64-65 2.2218571076093667 2314 66-67 1.2207707607604696 2314 68-69 1.1307553720575818 2314 70-71 1.2084404311584827 2314 72-73 1.1826638073832427 2314 74-75 0.2893349769575586 2314 76-77 0.30514116759844256 2314 78-79 0.08667317534792573 2314 80-81 -0.47511399973126345 2314 82-83 0.246615649397679 2314 84-85 0.4838279093152167 2314 86-87 -0.29344993484165727 2314 88-89 -0.2735796509300812 2314 90-91 -0.2775475442037987 2314 92-93 -0.4722396042089514 2314 94-95 -0.45027946107211747 2314 96-97 -1.968733216375682 2314 98-99 -1.6366124680467138 2314 100-101 -0.8015265713502515 2315 1 -0.4160760649211781 2315 2 -0.553736808891081 2315 3 -0.549663451746401 2315 4 -0.4482815399240323 2315 5 -0.5243467952087215 2315 6 -0.18366682266200485 2315 7 -0.4960825822418258 2315 8 -0.151637932775337 2315 9 -0.1437702997323953 2315 10-11 -0.345532380170674 2315 12-13 -0.14656770249689544 2315 14-15 -0.20561371706327947 2315 16-17 -0.698376347120238 2315 18-19 -0.36393164611958895 2315 20-21 0.07476472388157873 2315 22-23 -0.1417950472723959 2315 24-25 -0.4163755272209002 2315 26-27 -0.5686704749754696 2315 28-29 -0.3323510228972779 2315 30-31 -0.2816362915302406 2315 32-33 -0.8011474560187679 2315 34-35 -1.3358357510809924 2315 36-37 -1.34295432993801 2315 38-39 -1.7254561916955709 2315 40-41 -2.726715994811485 2315 42-43 -2.5251823801903512 2315 44-45 -2.110893835494714 2315 46-47 -2.0462632369169 2315 48-49 -1.9145953991780367 2315 50-51 -2.31124005665383 2315 52-53 -2.3119784151643437 2315 54-55 -2.556532788342558 2315 56-57 -2.0220289696288134 2315 58-59 -1.9649585882032596 2315 60-61 -1.2497219368627945 2315 62-63 -0.8916106204999785 2315 64-65 -1.8054030718247276 2315 66-67 -1.1425832765066097 2315 68-69 -1.2336338618968696 2315 70-71 -1.2387645377856167 2315 72-73 -0.9405245984138588 2315 74-75 -0.6789189912964098 2315 76-77 -0.93916525062102 2315 78-79 -1.3208492539136358 2315 80-81 -0.692850369641544 2315 82-83 -0.48457827744842064 2315 84-85 -0.3636531120788504 2315 86-87 0.047474840865728396 2315 88-89 0.08804905852471379 2315 90-91 0.6806995227389194 2315 92-93 -0.017788258453258265 2315 94-95 0.3868495934482432 2315 96-97 0.8066291024648962 2315 98-99 1.0124558549346538 2315 100-101 0.43104761774567635 2316 1 -0.06890873949298282 2316 2 0.01148058241326666 2316 3 0.30672653507836856 2316 4 0.3863693164659594 2316 5 0.6877744169124895 2316 6 0.041629620025744885 2316 7 0.003917417758174224 2316 8 0.30356628329844426 2316 9 0.2593917556035734 2316 10-11 0.3214636672601543 2316 12-13 -0.02700248510559078 2316 14-15 -0.30574546936894365 2316 16-17 -0.5965976909937609 2316 18-19 -0.11788421528954984 2316 20-21 -0.00823922868759297 2316 22-23 0.2877174691966502 2316 24-25 -0.017824802583227495 2316 26-27 0.18692899801530416 2316 28-29 0.2552642603701756 2316 30-31 0.2730409153208839 2316 32-33 0.5374559695411847 2316 34-35 0.9566543674960855 2316 36-37 1.5671247214453956 2316 38-39 2.144109025695734 2316 40-41 2.375062661314999 2316 42-43 1.3397715065026645 2316 44-45 1.0528084042944812 2316 46-47 0.4445141016865257 2316 48-49 0.7438367483845454 2316 50-51 1.5899457140971585 2316 52-53 1.8573232976156255 2316 54-55 0.8232432327378092 2316 56-57 0.1874571963790892 2316 58-59 0.12430359888501741 2316 60-61 0.17056791820967376 2316 62-63 0.3182049262720881 2316 64-65 0.5371529229051824 2316 66-67 0.8073508473405546 2316 68-69 -0.771842030539819 2316 70-71 -0.4950227723047185 2316 72-73 -0.5294574047379719 2316 74-75 -1.8986159609933786 2316 76-77 -2.6710493085920355 2316 78-79 -1.690743852069101 2316 80-81 -1.9563549809722467 2316 82-83 -1.8742357214536902 2316 84-85 -1.2394765639892604 2316 86-87 -0.9409968323885565 2316 88-89 -0.9481828255332552 2316 90-91 -0.9167971834534399 2316 92-93 -1.0415036734730236 2316 94-95 0.4797349689423136 2316 96-97 0.20550920786673998 2316 98-99 0.9413096098753648 2316 100-101 0.34277357294989486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1491.0 3 110.0 4 73.0 5 75.0 6 101.0 7 115.0 8 133.0 9 129.0 10 220.0 11 318.0 12 543.0 13 847.0 14 824.0 15 981.0 16 1389.0 17 1599.0 18 1406.0 19 935.0 20 1031.0 21 1089.0 22 978.0 23 843.0 24 810.0 25 845.0 26 780.0 27 868.0 28 954.0 29 1012.0 30 1129.0 31 1345.0 32 1650.0 33 2145.0 34 3072.0 35 6979.0 36 5962.0 37 6367.0 38 4710.0 39 370.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.82969303423849 20.886216056670605 14.058810507674144 11.225280401416764 2 14.429053617868934 29.909437999151557 33.43047383661951 22.23103454636 3 28.215721716035265 33.059131653694344 28.02021468884872 10.704931941421668 4 11.054349630230714 21.273260424543093 37.456429928259226 30.21596001696697 5 7.576540022132054 46.40538546661748 30.81335300627075 15.204721504979712 6 22.987551867219917 39.194098662978334 15.072383586906406 22.745965882895344 7 17.65389296647365 43.562128868070666 21.79397337070778 16.990004794747907 8 25.497934493951018 27.362422543523163 19.384405429330187 27.755237533195633 9 23.245177234332928 24.0732543985836 24.635756703920915 28.045811663162556 10-11 23.892377343180918 30.224152397812876 21.651775423455323 24.231694835550886 12-13 31.152892142850558 30.643897131370508 23.603721564975242 14.599489160803698 14-15 24.757268125363062 30.531197846071574 32.610439545240794 12.101094483324573 16-17 14.583967318634098 34.924060087974105 31.887385767375807 18.60458682601599 18-19 13.990889138894524 35.05468360967153 36.02755389978053 14.92687335165342 20-21 18.1651376146789 29.580932183855058 34.02978193720898 18.224148264257064 22-23 15.007192387134848 31.32007966951903 35.444637061079966 18.228090882266155 24-25 19.2818413190218 31.418981225332892 33.59153111283243 15.70764634281288 26-27 17.337661140106597 28.46946867565424 39.520129834203196 14.672740350035962 28-29 13.857816059094223 36.57607643147634 35.79958870127355 13.766518808155887 30-31 15.503933159345978 32.27496149838155 34.96315833156579 17.25794701070668 32-33 14.470541584824929 33.775048182882855 36.90117206591603 14.853238166376187 34-35 16.462901869461156 32.45913152763531 35.400552613826434 15.677413989077099 36-37 15.296535253769752 31.268802473364126 38.75276540992863 14.68189686293749 38-39 15.369093349377517 33.16425232563521 36.739019762114125 14.727634562873144 40-41 14.15807941930765 34.92764491847902 35.28502254441759 15.629253117795738 42-43 14.069760983466942 34.8036583785385 32.715272249273326 18.411308388721235 44-45 16.172099956484303 31.05724628959234 34.04131213717629 18.729341616747064 46-47 17.826686789937128 34.18486865862353 34.610790640827396 13.377653910611938 48-49 16.562184586037983 32.4345096441436 34.70687914957451 16.296426620243903 50-51 11.709684290463887 33.74174608025635 37.9814593578381 16.567110271441667 52-53 14.80068521690819 35.717394323811284 30.92550581045419 18.556414648826333 54-55 13.24772476877355 31.554193554360204 33.312347816426104 21.88573386044014 56-57 17.00350883689926 30.058418894022015 32.1340949700499 20.80397729902882 58-59 20.366975874391304 29.509896498731692 31.448462293321484 18.674665333555517 60-61 16.411457561842692 31.33332098948304 35.14294178640201 17.112279662272257 62-63 13.684415151627356 31.068796978561114 34.94834672492317 20.29844114488836 64-65 13.38561534757972 34.447798229695195 32.434909818154885 19.7316766045702 66-67 16.899153052251588 29.500624797519322 29.965289026704312 23.634933123524785 68-69 15.372578793378022 29.171141809562606 32.28121180697011 23.175067590089256 70-71 19.604902614233875 28.67603495519514 30.493223728060432 21.22583870251055 72-73 18.353762405569547 25.469374907421123 35.8965338468375 20.280328840171826 74-75 15.608333333333333 31.592592592592595 31.955555555555552 20.84351851851852 76-77 16.401259142671975 27.494676418850105 32.10073141375799 24.003333024719932 78-79 17.528770217848184 27.7851329957134 33.87062428826694 20.815472498171484 80-81 19.73577009961856 26.722956708513866 32.06495574565789 21.47631744620968 82-83 17.552354324439428 26.185494472938693 34.62791860314404 21.634232599477844 84-85 17.322878382506783 28.806043381257002 32.65351465945806 21.217563576778158 86-87 17.4596120909133 29.221867333240752 31.14937740128686 22.16914317455909 88-89 17.287037037037038 28.78981481481481 29.13703703703704 24.78611111111111 90-91 19.731236571089873 25.50566792620582 29.860339334666964 24.90275616803734 92-93 21.626276750409765 27.745418514848737 30.29475224328404 20.333552491457464 94-95 20.07591186817256 26.59970375856323 30.523977041288653 22.80040733197556 96-97 16.73331111851605 27.225184197859974 33.07693731719056 22.96456736643341 98-99 20.524377151741753 28.17254655166031 27.4136526857439 23.88942361085403 100-101 19.385620673059123 25.586797971196916 27.902521195068676 27.12506016067528 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2305.0 1 1318.0 2 500.5 3 597.5 4 355.5 5 278.5 6 495.0 7 785.0 8 1016.5 9 942.5 10 572.0 11 273.0 12 187.0 13 183.5 14 195.5 15 186.0 16 189.0 17 198.0 18 202.5 19 204.5 20 221.0 21 244.5 22 264.0 23 303.0 24 368.0 25 438.5 26 471.5 27 544.5 28 649.5 29 728.0 30 824.5 31 914.5 32 945.5 33 959.0 34 1002.5 35 1020.0 36 985.5 37 907.0 38 802.5 39 702.5 40 630.0 41 563.0 42 493.0 43 419.5 44 389.0 45 422.0 46 778.5 47 2700.0 48 6032.5 49 6145.5 50 4053.0 51 2695.5 52 1448.0 53 965.5 54 674.5 55 425.0 56 288.5 57 228.5 58 198.5 59 179.5 60 147.5 61 123.0 62 108.0 63 90.5 64 74.5 65 59.5 66 48.5 67 37.5 68 40.5 69 38.5 70 41.5 71 68.0 72 77.0 73 65.0 74 58.5 75 53.0 76 70.0 77 60.0 78 29.0 79 28.5 80 20.0 81 9.5 82 8.0 83 5.0 84 2.5 85 1.5 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.036881315925352216 2 0.020284723758943718 3 0.018440657962676108 4 0.009220328981338054 5 0.014752526370140887 6 0.005532197388802833 7 0.0036881315925352217 8 0.0073762631850704434 9 0.011064394777605666 10-11 0.0027660986944014164 12-13 0.006454230286936638 14-15 0.0027660986944014164 16-17 0.01383049347200708 18-19 0.012908460573873275 20-21 9.220328981338054E-4 22-23 0.0073762631850704434 24-25 0.011064394777605666 26-27 0.009220328981338054 28-29 0.017518625064542305 30-31 0.017518625064542305 32-33 0.01383049347200708 34-35 0.22405399424651473 36-37 0.3918639817068673 38-39 0.3881758501143321 40-41 0.41214870546581106 42-43 0.39647414619753635 44-45 0.4139927712620786 46-47 0.4195249686508815 48-49 0.4269012318359519 50-51 0.43980969240982515 52-53 0.4250571660396843 54-55 0.41030463966954345 56-57 0.4084605738732758 58-59 0.40385040938260675 60-61 0.4056944751788744 62-63 0.3946300804012687 64-65 0.4167588699564801 66-67 0.3881758501143321 68-69 0.4167588699564801 70-71 0.39831821199380396 72-73 0.4056944751788744 74-75 0.42044700154901526 76-77 0.4112266725676772 78-79 0.41030463966954345 80-81 0.4093826067714096 82-83 0.407538540975142 84-85 0.402928376484473 86-87 0.40661650807700817 88-89 0.42044700154901526 90-91 0.44257579110422657 92-93 0.4305893634284871 94-95 0.40200634358633913 96-97 0.3872538172161983 98-99 0.3725012908460574 100-101 0.37987755403112783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 54228.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.28464999631187 #Duplication Level Percentage of deduplicated Percentage of total 1 87.12008092095756 33.35361805709228 2 6.762680025046963 5.178136755919451 3 2.0326573864457393 2.334587298074795 4 1.151196955830644 1.762926901231836 5 0.7128751023553779 1.364608689238032 6 0.35643755117768894 0.8187652135428192 7 0.3227204855257454 0.8648668584495095 8 0.1974856702470979 0.6048535811757764 9 0.18303549925340784 0.6306705023235228 >10 0.8381099176340253 6.6681419193036815 >50 0.10115119695583064 2.607509035922402 >100 0.14450170993690092 13.435863391605812 >500 0.04816723664563364 11.997492070517076 >1k 0.02890034198738018 18.37795972560301 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2547 4.696835583093605 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2265 4.176809028546138 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1573 2.9007154975289517 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1419 2.61672936490374 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1132 2.0874824813749355 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1030 1.8993877701556392 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 896 1.6522829534557792 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 838 1.545327137272258 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 701 1.2926901231835952 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 644 1.1875783727963414 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 612 1.1285682673157778 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 604 1.1138157409456368 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 569 1.0492734380762707 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 566 1.0437412406874678 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 556 1.0253005827247916 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 520 0.9589142140591577 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 493 0.9091244375599321 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 432 0.7966364239876079 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 430 0.7929482923950727 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 410 0.7560669764697204 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 402 0.7413144500995795 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 401 0.739470384303312 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 370 0.682304344619016 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 351 0.6472670944899314 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 348 0.6417348971011286 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 313 0.5771925942317622 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 298 0.549531607287748 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 265 0.4886774360109169 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 245 0.45179612008556463 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 227 0.41860293575274765 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 214 0.3946300804012687 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 210 0.3872538172161983 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 196 0.36143689606845175 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 193 0.3559046986796489 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 155 0.28583019842147966 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 140 0.2581692114774655 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 138 0.2544810798849303 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 134 0.24710481669985984 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 133 0.24526075090359223 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 122 0.2249760271446485 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 117 0.21575569816331047 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 116 0.21391163236704286 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 115 0.21206756657077527 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 111 0.2046913033857048 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 105 0.19362690860809914 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 102 0.1880947112192963 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 98 0.18071844803422588 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 92 0.1696540532566202 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 82 0.1512133952939441 No Hit GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 80 0.14752526370140887 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 77 0.14199306631260603 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 75 0.13830493472007083 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 75 0.13830493472007083 No Hit GTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.13277273733126796 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 69 0.12724053994246515 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67 0.12355240834992992 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 64 0.1180202109611271 No Hit GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.11248801357232426 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 60 0.11064394777605664 No Hit GAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 57 0.10511175038725382 No Hit TATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 57 0.10511175038725382 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 57 0.10511175038725382 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 56 0.1032676845909862 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 56 0.1032676845909862 No Hit GGTATCAACGCAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 55 0.1014236187947186 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0027660986944014164 0.0 16-17 0.0 0.0 0.0 0.0036881315925352217 0.0 18-19 0.0 0.0 0.0 0.005532197388802833 0.0 20-21 0.0 0.0 0.0 0.009220328981338054 0.0 22-23 0.0 0.0 0.0 0.02212878955521133 0.0 24-25 0.0 0.0 0.0 0.04610164490669027 0.0 26-27 0.0 0.0 0.0 0.08206092793390868 0.0 28-29 0.0 0.0 0.0 0.16688795456221878 0.0 30-31 0.0 0.0 0.0 0.3761894224385926 0.0 32-33 0.0 0.0 0.0 0.6786162130264808 0.0 34-35 0.0 0.0 0.0 1.1073615106587003 0.0 36-37 0.0 0.0 0.0 1.6808659732979274 0.0 38-39 0.0 0.0 0.0 2.3087703769270487 0.0 40-41 0.0 0.0 0.0 3.076823781072509 0.0 42-43 0.0 0.0 0.0 3.649406210813602 0.0 44-45 0.0 0.0 0.0 4.12886331784318 0.0 46-47 0.0 0.0 0.0 4.798259201888324 0.0 48-49 0.0 0.0 0.0 5.519288928228959 0.0 50-51 0.0 0.0 0.0 6.0651324039241725 0.0 52-53 0.0 0.0 0.0 6.468060780408646 0.0 54-55 0.0 0.0 0.0 6.835029873865899 0.0 56-57 0.0 0.0 0.0 7.249022645127978 0.0 58-59 0.0 0.0 0.0 7.555137567308401 0.0 60-61 0.0 0.0 0.0 7.877849081655233 0.0 62-63 0.0 0.0 0.0 8.1360182931327 0.0 64-65 0.0 0.0 0.0 8.45872980747953 0.0 66-67 0.0 0.0 0.0 8.837685328612526 0.0 68-69 0.0 0.0 0.0 9.249834034078336 0.0 70-71 0.0 0.0 0.0 9.71085048314524 0.0 72-73 0.0 0.0 0.0 10.065833148926753 0.0 74-75 0.0 0.0 0.0 10.446632735856015 0.0 76-77 0.0 0.0 0.0 10.808991664822601 0.0 78-79 0.0 0.0 0.0 11.349302943129011 0.0 80-81 0.0 0.0 0.0 11.839824444936195 0.0 82-83 0.0 0.0 0.0 12.239064689828133 0.0 84-85 0.0 0.0 0.0 12.621708342553662 0.0 86-87 0.0 0.0 0.0 13.018182488751199 0.0 88-89 0.0 0.0 0.0 13.540053109094933 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCAATG 15 6.674907E-4 94.67159 8 GGGCAAT 25 0.0050645117 56.80295 7 TGGGCAA 40 5.4345856E-4 47.335796 6 ACAAACT 25 0.0015929072 38.079407 88-89 CTTGGTA 25 0.0016369794 37.868637 16-17 ATGGGCA 75 6.964692E-6 37.868633 5 GAGTAAA 65 1.338554E-4 36.412148 1 AGTAAAA 75 3.102578E-4 31.557194 2 GTAAAAA 95 0.0012362432 24.913574 3 GAGTACT 965 0.0 22.564213 12-13 AGTACTT 860 0.0 22.291855 12-13 GTACTTT 970 0.0 21.227907 14-15 CATGGGC 230 8.950883E-8 20.58078 4 AGTACTC 120 0.0048014796 19.723248 2 TACTTTT 1005 0.0 19.07562 14-15 ACTTTTT 1095 0.0 19.020775 16-17 CATGGGG 175 9.978142E-5 18.934319 4 TGGTCGC 90 1.2094271E-4 18.493668 94-95 GTGGTCG 105 1.9100731E-5 18.116243 94-95 GCAGAGA 120 3.0044102E-6 17.83318 94-95 >>END_MODULE