FastQCFastQC Report
Thu 26 May 2016
SRR936279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265274
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28420.12545943669507528No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38950.064.611061
GTACATG200200.054.0855521
TACATGG202850.052.1471632
ACATGGG205700.048.9662253
GTATCAA54800.046.1405032
CATGGGA144050.045.514724
CATGGGG59800.043.0794564
ATGGGAG48450.041.6283725
ATGGGAT57300.040.805765
ATCAACG64050.040.2209474
TCAACGC63950.040.097775
TACCTGG22100.039.0345952
CAACGCA66700.038.355376
AACGCAG67150.038.1869357
CGTATAG1156.666469E-736.2138021
ATGGGGG34750.035.9540675
TATCAAC77200.032.9837763
GAGTACA119950.031.0490281
ACGCAGA82800.030.6099648
AGTACAT115450.030.5071532
Produced by FastQC (version 0.11.5)