FastQCFastQC Report
Thu 26 May 2016
SRR936279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2265274
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122900.5425392248354945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99400.43879901504188895No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94390.4166824852092948No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32890.1451921489409228No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25100.11080337301359572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69400.087.9925841
GTATCAA111400.054.8299182
GTACATG172300.047.1294361
TATCACG1800.046.3031922
TACATGG177900.045.377352
ACATGGG176850.044.9735153
CATGGGA122500.041.259794
ATCAACG155500.040.581764
TCAACGC155600.040.555685
CAACGCA158700.039.8513686
CATGGGG55850.039.5463264
AACGCAG161950.039.0148967
TATCAAC166450.038.0551343
TACCTGG22050.035.90862
ATGGGAT55000.035.3948825
ATGGGAG42250.034.3815
ATCACGC2450.034.018673
ACGCAGA190250.032.961198
CGCAGAG191800.032.6637959
TATAACG2750.030.3075432
Produced by FastQC (version 0.11.5)