FastQCFastQC Report
Thu 26 May 2016
SRR936278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694854
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62790.903643067464532No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25100.3612269627864271No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT15500.22306844315496493No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG14220.2046473072041033No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13650.19644414510098523No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG11610.16708545967929953No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT11340.16319975131466466No Hit
AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11260.1620484303177358No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10160.1462177666099641No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC9200.13240191464681791No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA8740.12578181891447698No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT8720.1254939886652448No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG8700.12520615841601257No Hit
GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT8670.12477441304216425No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG8660.12463049791754816No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT8600.12376700716985152No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA8470.12189611054984212No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC7860.11311728794825963No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG7630.1098072400820892No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG7380.10620936196668652No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG7300.10505804096975768No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7220.10390671997282883No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC7210.10376280484821272No Hit
GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7040.1013162477297389No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG6960.10016492673281005No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12150.059.7418251
GTACATG54650.051.82171
GAACAAA12550.046.459791
TACATGG60850.045.0749472
ACATGGG58550.044.203563
ATGGGAT15900.043.7807925
GTACAAG2600.043.478561
CATGGGA36850.042.9476174
GTATCAA17650.041.125392
CATGGGT5600.039.3105054
TAGACGC1001.0619538E-535.6981854
CATGGGG15900.035.1743134
TGGGATC3050.035.1129726
ATGGGTA3900.035.0879635
TGGGTAT3250.034.782856
GGTACTC1555.116817E-934.5466353
GGGATAC1408.061397E-833.9982727
TCAACGC21100.033.8371435
ATGGGCG902.3927362E-433.053885
ATCAACG21900.032.8727574
Produced by FastQC (version 0.11.5)