##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936278_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 694854 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.128042725522196 31.0 28.0 33.0 12.0 34.0 2 28.409016858217697 31.0 28.0 34.0 16.0 34.0 3 28.701632285343397 31.0 28.0 34.0 16.0 34.0 4 31.746900787791393 35.0 33.0 37.0 16.0 37.0 5 32.480939880895846 35.0 33.0 37.0 23.0 37.0 6 32.05044512948044 36.0 33.0 37.0 17.0 37.0 7 32.105150146649514 36.0 33.0 37.0 17.0 37.0 8 31.74957473080676 35.0 32.0 37.0 13.0 37.0 9 32.869310675336116 38.0 33.0 39.0 10.0 39.0 10-11 32.99127298684328 38.0 33.0 39.0 12.0 39.0 12-13 33.01177009846673 38.0 33.0 39.0 11.5 39.0 14-15 34.02692076321069 39.0 33.0 41.0 12.5 41.0 16-17 34.093135392470934 39.0 33.0 41.0 14.0 41.0 18-19 34.09151850604587 39.0 33.0 41.0 14.5 41.0 20-21 34.170747380025155 39.0 33.0 41.0 17.0 41.0 22-23 34.0528693797546 39.0 33.0 41.0 17.5 41.0 24-25 34.04066609676278 39.0 32.5 41.0 18.0 41.0 26-27 34.09406076672222 39.0 33.0 41.0 18.0 41.0 28-29 33.959102199886594 39.0 33.0 41.0 17.5 41.0 30-31 33.85373186309642 39.0 32.5 41.0 17.0 41.0 32-33 33.66134324620712 38.5 32.0 41.0 15.5 41.0 34-35 33.57806747892363 38.0 32.0 41.0 15.0 41.0 36-37 33.44251742092584 38.0 32.0 40.0 9.5 41.0 38-39 33.164499304889944 38.0 31.0 40.0 8.5 41.0 40-41 33.225711444418536 38.0 31.5 40.0 8.0 41.0 42-43 33.12463697409815 38.0 32.0 41.0 4.5 41.0 44-45 33.03461590492391 38.0 31.0 40.0 2.0 41.0 46-47 33.00574797007717 38.0 32.0 40.0 2.0 41.0 48-49 32.89617027461884 38.0 31.0 40.0 2.0 41.0 50-51 32.75737996759031 38.0 31.0 40.0 2.0 41.0 52-53 32.61672308139552 38.0 31.0 40.0 2.0 41.0 54-55 32.46665702435332 38.0 31.0 40.0 2.0 41.0 56-57 32.28632273830186 38.0 30.5 40.0 2.0 41.0 58-59 32.060469537485574 37.0 30.5 40.0 2.0 41.0 60-61 31.85827094612681 37.0 30.5 40.0 2.0 41.0 62-63 31.603085684186894 36.5 30.0 40.0 2.0 41.0 64-65 31.334229924559693 36.0 30.0 40.0 2.0 41.0 66-67 31.04428124469313 36.0 30.0 39.0 2.0 41.0 68-69 30.779211172418954 35.0 30.0 39.0 2.0 41.0 70-71 30.46007722485587 35.0 30.0 39.0 2.0 40.0 72-73 30.13093254122449 35.0 29.0 38.0 2.0 40.0 74-75 29.7880885768809 35.0 29.0 37.0 2.0 39.5 76-77 29.48577183120483 35.0 29.0 37.0 2.0 39.0 78-79 29.147761256321473 34.5 29.0 36.0 2.0 39.0 80-81 28.852032945050325 34.0 29.0 36.0 2.0 38.5 82-83 28.55460859403558 34.0 28.0 35.5 2.0 37.0 84-85 28.51741804753229 34.0 29.0 35.0 2.0 37.0 86-87 28.407484162140534 34.0 29.0 35.0 2.0 36.5 88-89 28.234957991175122 34.0 29.0 35.0 2.0 36.0 90-91 28.075494996071118 34.0 28.0 35.0 2.0 36.0 92-93 27.941155264271345 34.0 28.0 35.0 2.0 36.0 94-95 27.819560655907573 34.0 28.0 35.0 2.0 35.0 96-97 27.72407153157354 34.0 28.0 35.0 2.0 35.0 98-99 27.626972716570677 34.0 27.5 35.0 2.0 35.0 100-101 27.549070740040353 34.0 27.0 35.0 2.0 35.0 102-103 26.660852495632177 32.5 25.5 34.5 2.0 35.0 104-105 27.270595693483813 34.0 27.0 35.0 2.0 35.0 106-107 27.385401825419443 34.0 27.0 35.0 2.0 35.0 108-109 27.3498403981268 34.0 27.0 35.0 2.0 35.0 110-111 27.23852852541685 34.0 27.0 35.0 2.0 35.0 112-113 27.221514879384735 34.0 27.0 35.0 2.0 35.0 114-115 27.12291574920775 34.0 26.5 35.0 2.0 35.0 116-117 27.02672935609495 34.0 26.0 35.0 2.0 35.0 118-119 26.85235099747573 34.0 25.0 35.0 2.0 35.0 120-121 26.68281970025358 34.0 25.0 35.0 2.0 35.0 122-123 26.42337167232253 34.0 25.0 35.0 2.0 35.0 124-125 24.561964096054712 31.5 13.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6010234754448476 1101 2 0.8137442303785285 1101 3 0.5165117975310949 1101 4 0.5997890139969435 1101 5 0.6476410948980131 1101 6 0.472417844437345 1101 7 0.3946737665390927 1101 8 0.4448287915837277 1101 9 0.36253389515183443 1101 10-11 0.6756434570793814 1101 12-13 0.6235274256762864 1101 14-15 0.623498104368025 1101 16-17 0.45186660431100734 1101 18-19 0.4847827836611671 1101 20-21 0.39839358540216807 1101 22-23 0.6120065686264624 1101 24-25 0.7705756791112108 1101 26-27 0.7778424142919533 1101 28-29 0.9118221867676013 1101 30-31 0.8085113575507208 1101 32-33 0.8627305126038038 1101 34-35 0.9864526034239915 1101 36-37 0.8016931846849431 1101 38-39 0.8376756242844223 1101 40-41 0.8117033366411732 1101 42-43 0.928200302300759 1101 44-45 0.9267557687278796 1101 46-47 0.8149652878017761 1101 48-49 0.6992922858557051 1101 50-51 0.7235653564188169 1101 52-53 0.7670683446113671 1101 54-55 0.6064760085472614 1101 56-57 0.7205690430042608 1101 58-59 0.6275131607002535 1101 60-61 0.6112283381791741 1101 62-63 0.6773736197361018 1101 64-65 0.8338627794407394 1101 66-67 0.7381252603460169 1101 68-69 0.7159861803719068 1101 70-71 0.6957599190641091 1101 72-73 0.7538159410536522 1101 74-75 0.662261530312076 1101 76-77 0.8101315745812769 1101 78-79 0.6409988314354109 1101 80-81 0.7291542622304199 1101 82-83 0.7753190437581132 1101 84-85 0.5815580515459899 1101 86-87 0.5298487565554382 1101 88-89 0.7694322826874576 1101 90-91 0.625756104632746 1101 92-93 0.617812600781491 1101 94-95 0.7286177038575765 1101 96-97 0.7467836487441559 1101 98-99 0.6884112004101475 1101 100-101 0.6907483921673503 1101 102-103 0.7162124717717049 1101 104-105 0.8014447749809221 1101 106-107 0.840683901464164 1101 108-109 0.5482110824370849 1101 110-111 0.8315169310352175 1101 112-113 0.8365598566218644 1101 114-115 0.7066560079102651 1101 116-117 0.6690609023807639 1101 118-119 0.823239149392144 1101 120-121 0.8367736937057906 1101 122-123 0.7517112696690695 1101 124-125 0.5103038123276598 1102 1 0.2311604617462173 1102 2 0.09410952718218013 1102 3 0.23432001670917657 1102 4 0.08928673089192429 1102 5 0.13074611772906763 1102 6 -0.04173740670420756 1102 7 0.16727650626512514 1102 8 -0.1149885600144458 1102 9 0.03376677186415833 1102 10-11 0.1318078406410237 1102 12-13 0.1080023115210409 1102 14-15 0.13080404044108462 1102 16-17 0.18611317965347496 1102 18-19 0.08615264667486144 1102 20-21 0.12670408768526897 1102 22-23 0.3836960663433544 1102 24-25 0.2970596973760493 1102 26-27 0.14222597593578712 1102 28-29 0.2109089447584651 1102 30-31 0.1957259694228668 1102 32-33 0.48784466785494374 1102 34-35 0.26544803721394317 1102 36-37 0.301693184684936 1102 38-39 0.22945644620222794 1102 40-41 0.356908816093231 1102 42-43 0.40719573609071347 1102 44-45 0.3199064536593781 1102 46-47 0.34373241108944796 1102 48-49 0.26093612147214174 1102 50-51 0.23224115550557656 1102 52-53 0.26889482862963376 1102 54-55 0.36446687612716744 1102 56-57 0.29591150875768335 1102 58-59 0.3439515168646352 1102 60-61 0.30529226511981733 1102 62-63 0.34769325443929233 1102 64-65 0.31879428629005346 1102 66-67 0.14177822838254528 1102 68-69 0.06895421690158798 1102 70-71 0.2099151702056652 1102 72-73 0.18943237940981916 1102 74-75 0.2887455485769159 1102 76-77 0.2375288348552509 1102 78-79 0.2222773702481966 1102 80-81 0.4401131663400051 1102 82-83 0.4164149341690724 1102 84-85 0.29662654469667515 1102 86-87 0.253593048792883 1102 88-89 0.3196605931897416 1102 90-91 0.2262127256373141 1102 92-93 0.26256145922897645 1102 94-95 0.30533003262469904 1102 96-97 0.3846831921231555 1102 98-99 0.3751691912777275 1102 100-101 0.4596981638568458 1102 102-103 0.5349339329589178 1102 104-105 0.5324950032914231 1102 106-107 0.33931403845046404 1102 108-109 0.3089416760443875 1102 110-111 0.358000949300056 1102 112-113 0.37126305296889583 1102 114-115 0.2655601174993052 1102 116-117 0.29965450968670027 1102 118-119 0.44880992564785416 1102 120-121 0.4217052005551061 1102 122-123 0.4398391235503496 1102 124-125 0.6002581502272015 1103 1 0.011374105531977818 1103 2 0.230472596971655 1103 3 0.14159811510497988 1103 4 0.036056937098479125 1103 5 0.33134442004624276 1103 6 0.13373718414425895 1103 7 -0.10670606817988926 1103 8 0.04726022572269173 1103 9 0.013081300223277026 1103 10-11 0.25276005539831203 1103 12-13 0.10064277753207307 1103 14-15 0.09733068774602316 1103 16-17 -0.023379883779043098 1103 18-19 -0.03156624264336472 1103 20-21 -0.02503742909420481 1103 22-23 -0.08202910514919637 1103 24-25 -0.041416127470945696 1103 26-27 0.04075265647973936 1103 28-29 0.01641664997823966 1103 30-31 0.021215725573121347 1103 32-33 0.3024332726887309 1103 34-35 0.19354830271059598 1103 36-37 -0.034605484507899575 1103 38-39 0.021834323974054826 1103 40-41 0.10032478993412042 1103 42-43 0.12893820849194526 1103 44-45 0.03448300934928028 1103 46-47 0.1684472826414165 1103 48-49 -0.034648490524652686 1103 50-51 -0.08226352080870925 1103 52-53 0.03391367099774101 1103 54-55 0.15696607631331005 1103 56-57 0.08482172601620874 1103 58-59 0.07797996115396444 1103 60-61 0.1767524136151266 1103 62-63 0.19618025590596844 1103 64-65 -0.009975745411662018 1103 66-67 -0.02841385159053189 1103 68-69 0.19849331637350076 1103 70-71 0.12442857176208122 1103 72-73 0.3068537795203383 1103 74-75 0.19412029772620443 1103 76-77 0.11507587845237666 1103 78-79 0.01515674169837311 1103 80-81 0.08036727862971915 1103 82-83 0.09749528533929563 1103 84-85 0.01921993594899618 1103 86-87 -8.87956402060297E-5 1103 88-89 -0.10654338635164251 1103 90-91 -0.1554177312745324 1103 92-93 -0.07825854733572868 1103 94-95 -0.1985427284158483 1103 96-97 -0.021765177295286264 1103 98-99 -0.2110740103044506 1103 100-101 -0.09109978030991783 1103 102-103 -0.11439318467812143 1103 104-105 -0.06531412995964558 1103 106-107 -0.14762759798946945 1103 108-109 -0.1541260359893819 1103 110-111 -0.1903597438996556 1103 112-113 -0.2785767765149423 1103 114-115 -0.10421855219211196 1103 116-117 -0.12905112141837094 1103 118-119 -0.17762340311511338 1103 120-121 -0.3309271218966039 1103 122-123 -0.22136720349665495 1103 124-125 -0.08737485777924903 1104 1 0.20155271272776787 1104 2 0.3738791243464554 1104 3 0.460235412590972 1104 4 0.5207800415788206 1104 5 0.3441862831464846 1104 6 0.3107420084270558 1104 7 0.2867905925923395 1104 8 0.3835751146153932 1104 9 0.4688072031310071 1104 10-11 0.6550955118738955 1104 12-13 0.5651931692546768 1104 14-15 0.5687712769609163 1104 16-17 0.7233567149585198 1104 18-19 0.5821735207779284 1104 20-21 0.5115309778623782 1104 22-23 0.5312757288622763 1104 24-25 0.5026462276718107 1104 26-27 0.4584107905181156 1104 28-29 0.5039353204677113 1104 30-31 0.48073009255053734 1104 32-33 0.615920090321687 1104 34-35 0.5643427928417069 1104 36-37 0.5767021078716894 1104 38-39 0.47335114029077374 1104 40-41 0.474441339570447 1104 42-43 0.5910478450397534 1104 44-45 0.5768142309858675 1104 46-47 0.4826608902091891 1104 48-49 0.4268717022177526 1104 50-51 0.19573362925910232 1104 52-53 0.4020041592060082 1104 54-55 0.4233660625786939 1104 56-57 0.4799236196759722 1104 58-59 0.5455576904790789 1104 60-61 0.4585323049972274 1104 62-63 0.4262579136966451 1104 64-65 0.4273457761545423 1104 66-67 0.5445770320601291 1104 68-69 0.5812054446090045 1104 70-71 0.5807381957198245 1104 72-73 0.5555218082027906 1104 74-75 0.6618639869576946 1104 76-77 0.7897830164451776 1104 78-79 0.6487687732193095 1104 80-81 0.6363186580999063 1104 82-83 0.6442072762286699 1104 84-85 0.6212766942369754 1104 86-87 0.5693310886456793 1104 88-89 0.49999142803078556 1104 90-91 0.5241880853340497 1104 92-93 0.3732754372471696 1104 94-95 0.42356656476663446 1104 96-97 0.4326702442714847 1104 98-99 0.5038612367182864 1104 100-101 0.38829543890022933 1104 102-103 0.3759067449319886 1104 104-105 0.4186449866758366 1104 106-107 0.381838869525307 1104 108-109 0.4701264290875038 1104 110-111 0.4618483812684495 1104 112-113 0.3528148259137929 1104 114-115 0.42639532777881684 1104 116-117 0.36981291163626295 1104 118-119 0.4802330816251583 1104 120-121 0.3926008423476226 1104 122-123 0.2996810908360992 1104 124-125 0.2982015095300845 1105 1 0.006755335174798205 1105 2 0.12426366740322337 1105 3 0.0854534006874168 1105 4 0.17772825644717827 1105 5 0.06221375808488716 1105 6 0.24369891549541478 1105 7 0.10881104690766108 1105 8 0.17350932841972977 1105 9 0.011882454631006567 1105 10-11 0.006765160055174135 1105 12-13 -0.02069333710133492 1105 14-15 -0.08925542648043461 1105 16-17 0.016981658100796437 1105 18-19 -0.09499233662048567 1105 20-21 -0.308240080390469 1105 22-23 -0.34397279765873634 1105 24-25 -0.16182970885326853 1105 26-27 -0.23823938539928236 1105 28-29 -0.05143044145794562 1105 30-31 -0.03607086281996885 1105 32-33 0.021466350409411916 1105 34-35 -0.10684838251796691 1105 36-37 -0.17798513847856867 1105 38-39 0.06162004558469647 1105 40-41 -0.1697402065417748 1105 42-43 -0.10152866576844133 1105 44-45 0.02951101496830688 1105 46-47 -0.07345949226046855 1105 48-49 -0.0019199244676855187 1105 50-51 -0.0023819892163317036 1105 52-53 -0.024065529478868086 1105 54-55 -0.02719182621964933 1105 56-57 -0.05856443899638464 1105 58-59 0.0658962812432975 1105 60-61 -0.022436229174463307 1105 62-63 0.0023681059762061807 1105 64-65 0.21038192239823772 1105 66-67 0.2911643631143299 1105 68-69 0.24703109913584598 1105 70-71 0.1428658077596765 1105 72-73 0.14528227444416686 1105 74-75 0.15517165194153648 1105 76-77 0.09469713114428302 1105 78-79 0.08422099103221115 1105 80-81 0.17014518699005876 1105 82-83 0.19105000494941393 1105 84-85 0.2515668735618206 1105 86-87 0.16497140708108304 1105 88-89 0.2223533501513728 1105 90-91 0.20553449232766852 1105 92-93 0.1010213639838824 1105 94-95 0.014321137092071723 1105 96-97 0.0019024600592061347 1105 98-99 0.22524311650297335 1105 100-101 0.21785566951355406 1105 102-103 0.2948754462613721 1105 104-105 0.09145710946601682 1105 106-107 0.02660747666776686 1105 108-109 -0.008357643516795576 1105 110-111 -0.18558430159644246 1105 112-113 -0.14338394386988185 1105 114-115 -0.10729217091526522 1105 116-117 -0.0652587813336325 1105 118-119 -0.13870242925857568 1105 120-121 -0.2077194896532717 1105 122-123 -0.18634878538684418 1105 124-125 -0.15651723961604702 1106 1 -0.07690349804485308 1106 2 -0.0768276956422973 1106 3 0.016171795560069313 1106 4 0.35109153362507683 1106 5 0.26671241318585004 1106 6 0.0826762130848877 1106 7 0.07491669548360846 1106 8 0.0015343860122953856 1106 9 0.25151257522147574 1106 10-11 0.5648676941280897 1106 12-13 0.4865998444536004 1106 14-15 0.3634068622931892 1106 16-17 0.2623364476144445 1106 18-19 0.1803878064922131 1106 20-21 0.04768278418013239 1106 22-23 0.09117282466272769 1106 24-25 -3.4460256973218897 1106 26-27 -0.6746189043243902 1106 28-29 -0.34994976553357304 1106 30-31 -0.07470453548827294 1106 32-33 -4.8325332763650195 1106 34-35 -0.935047183048205 1106 36-37 -0.2168697731679643 1106 38-39 0.4492172129313161 1106 40-41 -0.11482215529404272 1106 42-43 0.254501179407292 1106 44-45 0.21186548917069814 1106 46-47 0.07038437718779988 1106 48-49 0.15562872355485524 1106 50-51 0.24878997124750413 1106 52-53 0.45242608581565236 1106 54-55 0.13464730712483686 1106 56-57 0.18658362859605404 1106 58-59 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-1.9272169922152784 2216 70-71 -2.0781427001347055 2216 72-73 -2.2189764454282894 2216 74-75 -2.1051192276701514 2216 76-77 -1.9093774174397353 2216 78-79 -1.7352033086785497 2216 80-81 -1.7553201916171624 2216 82-83 -1.71846624501039 2216 84-85 -1.719443749882192 2216 86-87 -1.666874976936871 2216 88-89 -1.5755308463544146 2216 90-91 -1.7227818009585896 2216 92-93 -1.6247788638185021 2216 94-95 -1.7067952638333566 2216 96-97 -1.6957101844962956 2216 98-99 -1.6019621747405104 2216 100-101 -1.5714220479945666 2216 102-103 -1.6608562546574497 2216 104-105 -1.7899494094732304 2216 106-107 -1.7180046415389931 2216 108-109 -1.6417125836636686 2216 110-111 -1.6224533389198115 2216 112-113 -1.652953844168259 2216 114-115 -1.8475674123841017 2216 116-117 -1.526124921200232 2216 118-119 -1.5026780885907733 2216 120-121 -1.45189199664728 2216 122-123 -1.5626919478458206 2216 124-125 -1.5296071098566948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32590.0 3 6275.0 4 6594.0 5 5964.0 6 6744.0 7 5086.0 8 4110.0 9 3785.0 10 3948.0 11 4401.0 12 4430.0 13 4303.0 14 4060.0 15 3921.0 16 3548.0 17 3256.0 18 2758.0 19 2525.0 20 2435.0 21 2601.0 22 3141.0 23 5154.0 24 7482.0 25 9340.0 26 12035.0 27 9936.0 28 10938.0 29 12995.0 30 15006.0 31 18304.0 32 23941.0 33 31325.0 34 43194.0 35 63647.0 36 102815.0 37 155650.0 38 56532.0 39 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.821346642604055 21.697795508121132 10.083125375978263 21.39773247329655 2 17.660544517265496 25.625814919393136 36.138095196976636 20.575545366364732 3 14.81174462548967 35.68159642169434 27.80238726408713 21.704271688728856 4 9.85890561182637 20.95850351296819 36.032605410633025 33.14998546457241 5 9.966554125039217 46.2749009144367 32.861291724592505 10.897253235931577 6 25.925771350199252 43.529638642993554 17.746199555877286 12.798390450929908 7 25.74098406425532 38.51721155409849 22.139790718324594 13.602013663321594 8 24.320107994064934 38.85066337772162 19.687329550278978 17.141899077934468 9 25.656978216818644 19.805482637929448 18.566362715298883 35.971176429953026 10-11 21.18385263006404 32.91703245304742 27.38526300640426 18.513851910484277 12-13 22.08975501081533 32.017839898567594 26.74804669229341 19.14435839832366 14-15 21.33500374908072 33.13345432094146 23.264521031551286 22.267020898426537 16-17 20.648881795344693 34.30437013333257 24.930217898614288 20.11653017270845 18-19 19.83174616424142 33.11467482582396 26.231609154873837 20.821969855060786 20-21 21.82686142711271 32.26133054322852 26.017991282841358 19.893816746817418 22-23 21.822078912626843 31.99387517132451 25.650673018127446 20.533372897921204 24-25 21.387488297758278 32.7074867722393 26.043703240504545 19.86132168949788 26-27 20.620682062348845 32.82729976390537 26.258889527717194 20.293128646028595 28-29 20.892032968219084 30.879497906745474 27.564326740553007 20.664142384482428 30-31 20.751340394314468 32.19407312209875 26.748808918164528 20.305777565422254 32-33 20.560091300149242 32.73975283909788 26.50956823837053 20.190587622382353 34-35 20.8309527338876 32.18709568786491 26.344924753868803 20.637026824378683 36-37 20.939218886269632 32.26210110325334 25.83679449208035 20.96188551839667 38-39 20.346965473272764 33.04159297002388 26.35794909139055 20.25349246531281 40-41 19.828380843416408 32.63922918892859 27.716961513414716 19.815428454240287 42-43 20.617699884291905 32.49181120583952 26.03453685101288 20.8559520588557 44-45 19.891876177859587 32.8505443249385 26.482708895353902 20.77487060184802 46-47 20.38864503130172 32.28193135209038 26.60300784341944 20.72641577318846 48-49 20.078836761993717 33.14921778475607 26.16724244750872 20.6047030057415 50-51 20.366410016550336 32.66640282075268 26.081600345398286 20.885586817298698 52-53 20.30866938138759 32.616875355740454 26.280503732081655 20.793951530790302 54-55 20.48090816461635 33.01746271148389 26.046409882967886 20.455219240931882 56-57 20.45511784506939 33.25760988295366 25.659527986494986 20.627744285481967 58-59 20.10394996931008 32.149438694631314 26.664973264148482 21.08163807191012 60-61 20.455564310411205 33.58590492226767 25.89499210264049 20.06353866468063 62-63 19.837936944936555 33.429876535036904 26.54417553065181 20.18801098937473 64-65 20.394817321073667 32.38713799897244 26.532806219444975 20.685238460508913 66-67 20.62699502341499 33.0247793061564 25.607969443940743 20.740256226487865 68-69 20.391204764745375 32.79864028892421 26.37613540120328 20.43401954512714 70-71 21.180738947662313 31.724335938680554 26.717881336052372 20.377043777604758 72-73 20.788683665921187 32.78523258123289 25.557671107887415 20.868412644958507 74-75 20.62673687815984 32.87335594722913 25.8282687129508 20.671638461660237 76-77 20.32156441722206 32.42779408018674 27.131853787779576 20.118787714811624 78-79 20.401623938266166 32.17639964654445 26.405762937250127 21.01621347793925 80-81 20.452959115093407 33.29941728682182 25.558355508287367 20.689268089797412 82-83 21.467675223859974 31.700544287001296 25.7746951158085 21.057085373330224 84-85 20.11747800075843 31.75021785167665 26.3079195830488 21.824384564516116 86-87 20.486188815340213 32.21232964934335 26.67994044787858 20.621541087437855 88-89 20.80619929682969 31.544297858683777 26.902884494993906 20.746618349492625 90-91 20.689382229936072 31.902673079524618 26.631421852648185 20.776522837891125 92-93 20.50763218433814 32.12180697082191 26.312380955122194 21.05817988971776 94-95 20.117693873600693 31.639259210754496 26.753625044703668 21.489421870941143 96-97 20.80438538483292 32.07323558131318 26.631009270299423 20.491369763554477 98-99 20.24012290378174 32.3894639509824 26.573841210904476 20.796571934331386 100-101 19.759416913061532 32.41762472233356 26.802196434212394 21.02076193039252 102-103 20.557528396314325 32.284045894632314 26.246433595619212 20.911992113434145 104-105 19.324073347835192 33.63378035801791 26.153642458446274 20.888503835700618 106-107 19.73935494270011 32.88141520580612 26.674850651864496 20.704379199629276 108-109 20.33712190716735 33.434721757495296 25.213768389694213 21.01438794564314 110-111 19.912038696065775 33.86582159340636 25.29412780581736 20.928011904710505 112-113 19.315064747414564 33.23928479939671 26.897161130251824 20.548489322936906 114-115 19.558655169758804 33.86742673095839 25.73578459344308 20.83813350583972 116-117 19.355084759178588 34.08514919025806 25.977583683408383 20.58218236715497 118-119 19.69003337415755 32.62385064956559 26.203678784887074 21.482437191389785 120-121 19.548449630532037 33.059341541813666 26.00228394453195 21.38992488312234 122-123 19.80295056062379 33.15941115433381 26.144741822347523 20.89289646269488 124-125 20.30289816277952 32.9640471235684 25.33632964622784 21.396725067424235 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5227.0 1 5734.0 2 5808.0 3 4396.0 4 2740.5 5 1734.5 6 1250.5 7 1047.5 8 1003.0 9 985.5 10 841.0 11 678.0 12 643.0 13 630.5 14 608.0 15 613.0 16 637.5 17 711.0 18 789.5 19 825.5 20 981.0 21 1203.5 22 1389.0 23 1730.0 24 2001.0 25 2242.0 26 2724.0 27 3361.5 28 3977.5 29 5311.0 30 7851.0 31 10148.5 32 13414.5 33 18179.0 34 22654.0 35 25913.0 36 31057.0 37 35842.5 38 35428.0 39 34658.5 40 32256.5 41 29038.5 42 25800.0 43 24063.5 44 24524.5 45 24034.0 46 20920.0 47 16954.5 48 15995.0 49 14689.0 50 13800.5 51 13077.5 52 12849.5 53 13554.0 54 13507.0 55 12756.0 56 11188.5 57 10011.0 58 9455.0 59 9234.5 60 8879.5 61 7768.5 62 6525.5 63 5255.5 64 4219.5 65 3640.0 66 3184.5 67 2942.5 68 2321.5 69 1487.5 70 1223.0 71 1056.5 72 894.0 73 795.5 74 690.5 75 619.0 76 435.5 77 276.5 78 195.0 79 124.5 80 75.5 81 52.0 82 36.0 83 24.0 84 21.0 85 11.5 86 5.5 87 4.5 88 4.5 89 6.0 90 3.0 91 1.5 92 1.5 93 1.0 94 1.5 95 1.5 96 0.5 97 1.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.439151246161064E-4 7 1.439151246161064E-4 8 1.439151246161064E-4 9 8.634907476966384E-4 10-11 5.756604984644256E-4 12-13 0.0010074058723127449 14-15 0.0024465571184738087 16-17 0.00179893905770133 18-19 0.0015830663707771704 20-21 0.002518514680781862 22-23 0.0907384860704551 24-25 0.0012232785592369044 26-27 7.915331853885852E-4 28-29 4.317453738483192E-4 30-31 3.59787811540266E-4 32-33 4.317453738483192E-4 34-35 1.439151246161064E-4 36-37 0.0 38-39 1.439151246161064E-4 40-41 2.158726869241596E-4 42-43 2.878302492322128E-4 44-45 3.59787811540266E-4 46-47 5.756604984644256E-4 48-49 7.19575623080532E-5 50-51 5.756604984644256E-4 52-53 7.19575623080532E-5 54-55 2.878302492322128E-4 56-57 1.439151246161064E-4 58-59 7.19575623080532E-5 60-61 2.158726869241596E-4 62-63 1.439151246161064E-4 64-65 1.439151246161064E-4 66-67 0.0 68-69 7.19575623080532E-5 70-71 1.439151246161064E-4 72-73 0.0 74-75 1.439151246161064E-4 76-77 1.439151246161064E-4 78-79 0.0 80-81 1.439151246161064E-4 82-83 0.0 84-85 7.19575623080532E-5 86-87 7.19575623080532E-5 88-89 1.439151246161064E-4 90-91 0.0 92-93 7.19575623080532E-5 94-95 7.19575623080532E-5 96-97 7.19575623080532E-5 98-99 2.158726869241596E-4 100-101 7.19575623080532E-5 102-103 2.158726869241596E-4 104-105 7.19575623080532E-5 106-107 1.439151246161064E-4 108-109 2.158726869241596E-4 110-111 4.317453738483192E-4 112-113 0.0 114-115 7.19575623080532E-5 116-117 4.317453738483192E-4 118-119 7.19575623080532E-4 120-121 5.037029361563724E-4 122-123 4.317453738483192E-4 124-125 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 694854.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.20966670271964 #Duplication Level Percentage of deduplicated Percentage of total 1 67.02651217747864 17.567425444174944 2 9.9649364573483 5.223553265217574 3 6.124167016586131 4.815371290095341 4 4.175909597413886 4.377967949156244 5 2.7901957509306374 3.656505033361827 6 1.952001704073683 3.0696788440147205 7 1.3537274224531388 2.4836521184179965 8 0.9099820127761856 1.908026020826703 9 0.681668461748658 1.6079672865769126 >10 4.046582625559287 20.57507386767904 >50 0.5822742008799349 10.854248519403441 >100 0.3694353357855234 18.165323245097635 >500 0.017644672753935445 3.1570188654215197 >1k 0.004411168188483861 1.6307530728961512 >5k 5.513960235604827E-4 0.9074351776599552 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6279 0.903643067464532 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2510 0.3612269627864271 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1550 0.22306844315496493 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1422 0.2046473072041033 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1365 0.19644414510098523 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 1161 0.16708545967929953 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 1134 0.16319975131466466 No Hit AAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1126 0.1620484303177358 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1016 0.1462177666099641 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 920 0.13240191464681791 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 874 0.12578181891447698 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 872 0.1254939886652448 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 870 0.12520615841601257 No Hit GTACATGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 867 0.12477441304216425 No Hit GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG 866 0.12463049791754816 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 860 0.12376700716985152 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 847 0.12189611054984212 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 786 0.11311728794825963 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 763 0.1098072400820892 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 738 0.10620936196668652 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 730 0.10505804096975768 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 722 0.10390671997282883 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 721 0.10376280484821272 No Hit GAAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 704 0.1013162477297389 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 696 0.10016492673281005 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.1587268692415963E-4 0.0 24-25 0.0 0.0 0.0 7.19575623080532E-4 0.0 26-27 0.0 0.0 0.0 0.0011513209969288513 0.0 28-29 0.0 0.0 0.0 0.0015830663707771704 0.0 30-31 0.0 0.0 0.0 0.006620095732340895 0.0 32-33 0.0 0.0 0.0 0.017125899829316664 0.0 34-35 0.0 0.0 0.0 0.027343873677060218 0.0 36-37 0.0 0.0 0.0 0.04123168320251448 0.0 38-39 0.0 0.0 0.0 0.06447397582801567 0.0 40-41 0.0 0.0 0.0 0.0944083217481658 0.0 42-43 0.0 0.0 0.0 0.13002731509065213 0.0 44-45 0.0 0.0 0.0 0.17269814953932766 0.0 46-47 0.0 0.0 0.0 0.22472346708805016 0.0 48-49 0.0 0.0 0.0 0.2758133363267679 0.0 50-51 0.0 0.0 0.0 0.3239529455108555 0.0 52-53 0.0 0.0 0.0 0.38144703779499 0.0 54-55 0.0 0.0 0.0 0.4455612258114654 0.0 56-57 0.0 0.0 0.0 0.5267293560949495 0.0 58-59 0.0 0.0 0.0 0.6263905798916032 0.0 60-61 0.0 0.0 0.0 0.7238211192567072 0.0 62-63 0.0 0.0 0.0 0.8213236161841193 0.0 64-65 0.0 0.0 0.0 0.9317784743269809 0.0 66-67 0.0 0.0 0.0 1.0782840711861772 0.0 68-69 0.0 0.0 0.0 1.2365187507015862 0.0 70-71 0.0 0.0 0.0 1.4040359557547342 0.0 72-73 0.0 0.0 0.0 1.5651489377624652 0.0 74-75 0.0 0.0 0.0 1.7366238087425558 0.0 76-77 0.0 0.0 0.0 1.945228781873602 0.0 78-79 0.0 0.0 0.0 2.1862146580432724 0.0 80-81 0.0 0.0 0.0 2.450370869276136 0.0 82-83 0.0 0.0 0.0 2.715534486381312 0.0 84-85 0.0 0.0 0.0 3.0006303482458185 0.0 86-87 0.0 0.0 0.0 3.3250869966928303 0.0 88-89 0.0 0.0 0.0 3.6586822555529652 0.0 90-91 0.0 0.0 0.0 4.033077452241766 0.0 92-93 0.0 0.0 0.0 4.426037700006044 0.0 94-95 0.0 0.0 0.0 4.854473025988193 0.0 96-97 0.0 0.0 0.0 5.301401445483512 0.0 98-99 0.0 0.0 0.0 5.77862399871052 0.0 100-101 0.0 0.0 0.0 6.3175861403978395 0.0 102-103 0.0 0.0 0.0 6.854821300589764 0.0 104-105 0.0 0.0 0.0 7.416234201717195 0.0 106-107 0.0 0.0 0.0 8.007005788266312 0.0 108-109 0.0 0.0 0.0 8.628934999294817 0.0 110-111 0.0 0.0 0.0 9.277704381064224 0.0 112-113 0.0 0.0 0.0 9.908988075192774 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1215 0.0 59.741825 1 GTACATG 5465 0.0 51.8217 1 GAACAAA 1255 0.0 46.45979 1 TACATGG 6085 0.0 45.074947 2 ACATGGG 5855 0.0 44.20356 3 ATGGGAT 1590 0.0 43.780792 5 GTACAAG 260 0.0 43.47856 1 CATGGGA 3685 0.0 42.947617 4 GTATCAA 1765 0.0 41.12539 2 CATGGGT 560 0.0 39.310505 4 TAGACGC 100 1.0619538E-5 35.698185 4 CATGGGG 1590 0.0 35.174313 4 TGGGATC 305 0.0 35.112972 6 ATGGGTA 390 0.0 35.087963 5 TGGGTAT 325 0.0 34.78285 6 GGTACTC 155 5.116817E-9 34.546635 3 GGGATAC 140 8.061397E-8 33.998272 7 TCAACGC 2110 0.0 33.837143 5 ATGGGCG 90 2.3927362E-4 33.05388 5 ATCAACG 2190 0.0 32.872757 4 >>END_MODULE