FastQCFastQC Report
Thu 26 May 2016
SRR936278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694854
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103811.4939829086398007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97631.4050433616270468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84411.2147875668845542No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27650.3979253195635342No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22370.32193813376623004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18260.2627890175490103No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT14660.210979572687212No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.21069174243797975No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG12340.17759126377627532No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.1752886217824176No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG10540.15168654134537615No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT10250.14751300273150905No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC9250.13312149026989842No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA9230.1328336600206662No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG8920.1283722911575669No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT8460.12175219542522601No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG8270.11901780805752No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC8240.11858606268367168No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC7920.11398077869595627No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG7760.11167813670209857No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7620.10966332495747308No Hit
GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG7270.10462629559590936No Hit
GTCCCAGGGTGTGCTTGTCAAAGAGATATTCTGCCATGCCAGCTTCAGGG7210.10376280484821272No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA7170.10318714434974828No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT7110.10232365360205165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38950.083.6725851
TGGTACT1550.057.6169172
GTATCAA62250.052.3164672
ATCACGC350.007777793351.028453
GGTACTC1550.049.9310653
CCGATAG602.1852626E-549.6109923
CGATAGC602.1852626E-549.6109924
GTGGTAC1800.046.3402941
CTTACTA5400.046.273619
ATATAGG5550.046.128142
CACGCAG1256.202754E-1042.86395
GCTTACT6250.042.836148
GTACATG48900.042.2789231
TACCTGG2750.041.134992
ACATGGG50050.040.561083
AGGCTTA6350.040.2877126
GATGACC6400.039.0433549
CATGGGT4450.038.7969134
ATGGGAT16300.038.3496065
GCTATCG701.893568E-938.24930670-71
Produced by FastQC (version 0.11.5)