FastQCFastQC Report
Thu 26 May 2016
SRR936277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350431
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21600.069.684391
GTACATG106100.052.3721281
GTATCAA28450.052.0697862
TACATGG108450.050.4144132
ACATGGG107200.049.503833
TCAACGC32850.046.363195
ATCAACG32800.046.2524834
CAACGCA33300.045.9153186
CATGGGA69750.044.5240864
AACGCAG34750.044.3418357
CATGGGG34400.043.2364734
TATCAAC39750.037.8662343
ATGGGAG28000.036.9708945
ATGGGAT30050.035.8346185
ACGCAGA42600.034.496278
CGCAGAG43700.032.811079
ATGGGGG20300.032.530875
ATGGGGA20350.031.2815385
GAGTACA63050.030.8554151
AGTACAT65150.029.4042552
Produced by FastQC (version 0.11.5)