FastQCFastQC Report
Thu 26 May 2016
SRR936277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350431
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21070.15602426188379856No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13550.10033833642740725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29300.080.7083361
GTATCAA42350.055.5200042
GTACATG97300.047.260681
TACATGG97850.046.6595232
ACATGGG98800.045.2468953
TCAACGC60250.040.704835
ATCAACG60650.040.4363754
CAACGCA61550.040.0177736
CATGGGA67100.039.9204834
AACGCAG62950.039.3168037
CATGGGG31500.036.660384
TATCAAC68350.035.9680863
ATGGGAT29300.033.9277155
ATGGGAG25100.032.9645275
ACGCAGA75600.032.3445178
CGCAGAG76850.031.6635829
ATGGGCG2109.094947E-1131.1802225
ATGGGGG16750.028.7856465
CAGAGTA84750.028.5014489
TATTACG850.0071955228.0121772
Produced by FastQC (version 0.11.5)