##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936277_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1350431 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51896468608911 31.0 31.0 34.0 30.0 34.0 2 31.39508275506116 31.0 31.0 34.0 30.0 34.0 3 31.76772230495301 31.0 31.0 34.0 30.0 34.0 4 35.57803767834121 37.0 35.0 37.0 33.0 37.0 5 35.19046215615607 37.0 35.0 37.0 33.0 37.0 6 35.381629272432285 37.0 35.0 37.0 33.0 37.0 7 35.19245707481537 37.0 35.0 37.0 32.0 37.0 8 35.27323869194353 37.0 35.0 37.0 32.0 37.0 9 36.97136988117127 39.0 37.0 39.0 33.0 39.0 10-11 36.84944547333407 39.0 37.0 39.0 32.0 39.0 12-13 36.794836611422575 39.0 37.0 39.0 32.0 39.0 14-15 37.94682882724108 40.0 37.0 41.0 32.5 41.0 16-17 37.85741107838905 40.0 37.0 41.0 32.0 41.0 18-19 37.943780170923205 40.0 37.0 41.0 32.0 41.0 20-21 37.99614382371257 40.0 37.0 41.0 32.5 41.0 22-23 37.91378900513984 40.0 37.0 41.0 32.0 41.0 24-25 37.89104330395259 40.0 37.0 41.0 32.0 41.0 26-27 37.73714910276793 40.0 37.0 41.0 32.0 41.0 28-29 37.59446798836816 40.0 37.0 41.0 32.0 41.0 30-31 37.45308831032463 40.0 36.5 41.0 32.0 41.0 32-33 37.30577237933667 39.5 36.0 41.0 31.0 41.0 34-35 37.14603745026588 39.0 36.0 41.0 31.0 41.0 36-37 36.976804072181395 39.0 36.0 41.0 30.5 41.0 38-39 36.99277712078589 39.0 36.0 41.0 30.5 41.0 40-41 37.04915467728451 40.0 36.0 41.0 31.0 41.0 42-43 36.969150959952785 40.0 36.0 41.0 31.0 41.0 44-45 36.843655840246555 40.0 36.0 41.0 30.5 41.0 46-47 36.64052254428401 40.0 36.0 41.0 30.0 41.0 48-49 36.4200925482309 39.0 35.5 41.0 30.0 41.0 50-51 36.24560381093147 39.0 35.0 41.0 29.5 41.0 52-53 36.10116362850083 39.0 35.0 41.0 29.0 41.0 54-55 35.918749643632296 39.0 35.0 41.0 28.5 41.0 56-57 35.72075989073119 39.0 35.0 41.0 28.0 41.0 58-59 35.508974171949546 38.5 34.5 40.0 28.0 41.0 60-61 35.286499643447165 38.0 34.0 40.0 28.0 41.0 62-63 35.074568045312944 38.0 34.0 40.0 28.0 41.0 64-65 34.832308722178325 37.0 34.0 40.0 27.0 41.0 66-67 34.55225739041832 37.0 34.0 40.0 26.5 41.0 68-69 34.24355557596056 36.0 34.0 39.0 26.0 41.0 70-71 33.92121700405278 36.0 33.0 39.0 26.0 41.0 72-73 33.655419640100085 35.5 33.0 39.0 26.0 40.0 74-75 33.47582142293831 35.0 33.0 37.5 26.0 40.0 76-77 33.17366011295653 35.0 33.0 37.0 26.0 39.0 78-79 32.83776179604882 35.0 33.0 37.0 26.0 39.0 80-81 32.569986915288524 35.0 33.0 36.0 26.0 39.0 82-83 32.3200837362294 35.0 33.0 36.0 26.0 37.0 84-85 32.0724738990737 35.0 32.5 35.5 25.5 37.0 86-87 31.879752834465442 35.0 32.0 35.0 25.0 36.5 88-89 31.72689719060063 35.0 32.0 35.0 25.0 36.0 90-91 31.599336063819628 35.0 32.0 35.0 25.0 36.0 92-93 31.48092238700089 35.0 32.0 35.0 25.0 36.0 94-95 31.371204452504422 35.0 32.0 35.0 25.0 35.5 96-97 31.31829541827757 35.0 32.0 35.0 25.0 35.0 98-99 31.23972050404649 35.0 32.0 35.0 24.0 35.0 100-101 31.16600292795411 34.5 32.0 35.0 24.0 35.0 102-103 30.12396227574752 33.5 30.0 34.5 21.5 35.0 104-105 30.910518567775767 34.0 31.5 35.0 23.5 35.0 106-107 31.067475865112694 34.5 32.0 35.0 24.0 35.0 108-109 31.046163410052053 35.0 32.0 35.0 24.0 35.0 110-111 31.009417734041946 35.0 32.0 35.0 24.0 35.0 112-113 30.9379812815316 35.0 32.0 35.0 23.0 35.0 114-115 30.91938758811076 35.0 32.0 35.0 23.0 35.0 116-117 30.822244527858146 34.5 32.0 35.0 23.0 35.0 118-119 30.68859201247602 34.0 32.0 35.0 19.5 35.0 120-121 30.48662945385584 34.0 32.0 35.0 18.5 35.0 122-123 30.25338873293045 34.0 32.0 35.0 9.5 35.0 124-125 28.0913811960774 32.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2654777001413464 1101 2 0.6890239441976043 1101 3 0.451680113803409 1101 4 0.3629842699546657 1101 5 0.5981303644084406 1101 6 0.40309796717091473 1101 7 0.45809854560231855 1101 8 0.4590632465381219 1101 9 0.41020797671374254 1101 10-11 0.34429941589800706 1101 12-13 0.4729341815868935 1101 14-15 0.532137743931834 1101 16-17 0.5222753064383312 1101 18-19 0.5831436783344373 1101 20-21 0.4750948188812174 1101 22-23 0.3633289125959678 1101 24-25 0.3994744193607289 1101 26-27 0.4883175344658994 1101 28-29 0.5474434289551127 1101 30-31 0.43213217346092136 1101 32-33 0.36646683835081006 1101 34-35 0.3196411445043452 1101 36-37 0.31682243119602305 1101 38-39 0.4151418469942243 1101 40-41 0.36274210539875895 1101 42-43 0.37901501540586935 1101 44-45 0.5291539698850656 1101 46-47 0.6072955043922121 1101 48-49 0.5669401540307817 1101 50-51 0.475170663934378 1101 52-53 0.5198230556870698 1101 54-55 0.5294759765856085 1101 56-57 0.4732569336770922 1101 58-59 0.48000501675979734 1101 60-61 0.5543021400342454 1101 62-63 0.5414411353228914 1101 64-65 0.5544301300637784 1101 66-67 0.5669880944469625 1101 68-69 0.4143200684446242 1101 70-71 0.49830082771403994 1101 72-73 0.5196165263939676 1101 74-75 0.48489068734885876 1101 76-77 0.5420417158699635 1101 78-79 0.4495541009270809 1101 80-81 0.45518925881094674 1101 82-83 0.4272258231048909 1101 84-85 0.42445921465662906 1101 86-87 0.4312939690615032 1101 88-89 0.4409000178896392 1101 90-91 0.4748908248820918 1101 92-93 0.4733927307453243 1101 94-95 0.41795228701334963 1101 96-97 0.379125411631108 1101 98-99 0.4126230650447624 1101 100-101 0.4896188096794951 1101 102-103 0.5309079493795714 1101 104-105 0.4765130201039227 1101 106-107 0.5964005162616033 1101 108-109 0.5029228219758259 1101 110-111 0.536560970649802 1101 112-113 0.4267891333766727 1101 114-115 0.48125252265027285 1101 116-117 0.5311110122052725 1101 118-119 0.4386856631144802 1101 120-121 0.5773268484165044 1101 122-123 0.5918091849332754 1101 124-125 0.6045144872091441 1102 1 0.32462138695927223 1102 2 0.7018476700701761 1102 3 0.3544981746635649 1102 4 0.2939076669780505 1102 5 0.4581097058224657 1102 6 0.3790795328202279 1102 7 0.5213286991319919 1102 8 0.4884960752415495 1102 9 0.46112303720580883 1102 10-11 0.48315574474371203 1102 12-13 0.450935920249691 1102 14-15 0.44609721342213504 1102 16-17 0.5330557989550329 1102 18-19 0.45736104077079176 1102 20-21 0.44586061369289354 1102 22-23 0.5684539214067641 1102 24-25 0.33917466971772825 1102 26-27 0.4804367712294706 1102 28-29 0.5250842515044738 1102 30-31 0.41886725537734293 1102 32-33 0.4709756574089212 1102 34-35 0.4129761399227263 1102 36-37 0.4570906621370838 1102 38-39 0.37979817392548654 1102 40-41 0.2770509608312963 1102 42-43 0.29938656538284647 1102 44-45 0.28512790386635345 1102 46-47 0.2552991749025537 1102 48-49 0.23083283554830558 1102 50-51 0.22393519521334326 1102 52-53 0.292872085455798 1102 54-55 0.2278131953690803 1102 56-57 0.2292028036567828 1102 58-59 0.3035788216031676 1102 60-61 0.40845743969251913 1102 62-63 0.3280187542598725 1102 64-65 0.22012151828768367 1102 66-67 0.10773963535645237 1102 68-69 0.11117011269437427 1102 70-71 0.13039634278201362 1102 72-73 0.18249346509436037 1102 74-75 0.18569427501601155 1102 76-77 0.1917753471603234 1102 78-79 0.09922486015187104 1102 80-81 0.16240623226718753 1102 82-83 0.19455980386821636 1102 84-85 0.18358610658368235 1102 86-87 0.04004741397208633 1102 88-89 0.07361794465173688 1102 90-91 0.06317952122438086 1102 92-93 0.09821053228540677 1102 94-95 0.16219568588156363 1102 96-97 0.1353636693482727 1102 98-99 0.24454449628273522 1102 100-101 0.13047420914988805 1102 102-103 0.13794905509253041 1102 104-105 0.1549408964900394 1102 106-107 0.17694006735940349 1102 108-109 0.11500652840809522 1102 110-111 0.2271011831008174 1102 112-113 0.21273128759553828 1102 114-115 0.21994621081701382 1102 116-117 0.24669562669291167 1102 118-119 0.18644663439869902 1102 120-121 0.27874871432017656 1102 122-123 0.20588782853263865 1102 124-125 0.24299053795581926 1103 1 0.07129708914946775 1103 2 0.5805352602776637 1103 3 0.3521112467546992 1103 4 0.1579932995763329 1103 5 0.3417149585084971 1103 6 0.1792545960885974 1103 7 0.29511950837606804 1103 8 0.2928638578216365 1103 9 0.2592243952922715 1103 10-11 0.2311628076863812 1103 12-13 0.1603456694219787 1103 14-15 0.3696626598991344 1103 16-17 0.49354179980343105 1103 18-19 0.29166950146166926 1103 20-21 0.3169881761108968 1103 22-23 0.31414431814241794 1103 24-25 0.2926029811123385 1103 26-27 0.16814254936797113 1103 28-29 0.16212050785592425 1103 30-31 0.04642836007516138 1103 32-33 0.12139479800980979 1103 34-35 0.1214547573825655 1103 36-37 0.06543650467995832 1103 38-39 0.2198493796031613 1103 40-41 -0.003476106996849637 1103 42-43 0.07965753915750184 1103 44-45 0.0030511386888392167 1103 46-47 0.08044734829530142 1103 48-49 0.13207870028599444 1103 50-51 0.19898244128295062 1103 52-53 0.20159064334421828 1103 54-55 0.22688666021656445 1103 56-57 0.23346447136752602 1103 58-59 0.346902383963382 1103 60-61 0.18576002072658326 1103 62-63 0.046362245990657414 1103 64-65 0.12983818633331623 1103 66-67 0.08888520315636583 1103 68-69 0.058091993501633965 1103 70-71 0.09079402401452086 1103 72-73 0.07244820867854429 1103 74-75 0.194852920685598 1103 76-77 0.23090684686024332 1103 78-79 0.12689779258638367 1103 80-81 0.041752443848153575 1103 82-83 0.0019691958221415007 1103 84-85 0.019412745355801064 1103 86-87 -0.004946341272916044 1103 88-89 -0.09868902938032775 1103 90-91 -0.11711200573969549 1103 92-93 -0.18905771901246382 1103 94-95 -0.04322884582747477 1103 96-97 0.004840081772645277 1103 98-99 -0.03953308576914338 1103 100-101 -0.03229851460079658 1103 102-103 -0.05871640333552364 1103 104-105 0.0031434445602087635 1103 106-107 -0.03586653960451258 1103 108-109 -0.05112168409480944 1103 110-111 -0.10423363163811672 1103 112-113 -0.09461473749642124 1103 114-115 -0.11228591808628963 1103 116-117 -0.24290596975802003 1103 118-119 -0.2891856880854391 1103 120-121 -0.328654176784692 1103 122-123 -0.20424055995285073 1103 124-125 -0.12850310762839712 1104 1 0.1601942224225148 1104 2 0.4978909771139861 1104 3 0.2623515447891869 1104 4 0.11711094436189029 1104 5 0.39401784687695596 1104 6 0.27345544474489003 1104 7 0.39128818847592584 1104 8 0.292444940051638 1104 9 0.31682790739992583 1104 10-11 0.3545124494421259 1104 12-13 0.3692952060618282 1104 14-15 0.43354684691268375 1104 16-17 0.4996106970912848 1104 18-19 0.34004213732010413 1104 20-21 0.36292923391691545 1104 22-23 0.3767794809561238 1104 24-25 0.281303794987636 1104 26-27 0.2645593339035699 1104 28-29 0.3705661065141541 1104 30-31 0.4340625567500993 1104 32-33 0.35959592491406767 1104 34-35 0.2674290503622103 1104 36-37 0.3960762431268634 1104 38-39 0.2910638546806439 1104 40-41 0.33655759192339474 1104 42-43 0.312105889378941 1104 44-45 0.38659818191774775 1104 46-47 0.3619863687078819 1104 48-49 0.3238611103771163 1104 50-51 0.2926376147628318 1104 52-53 0.33054079831288163 1104 54-55 0.2971860086525737 1104 56-57 0.24740933189573866 1104 58-59 0.34863447754744925 1104 60-61 0.3066054307945265 1104 62-63 0.24490221708378357 1104 64-65 0.17111395325427736 1104 66-67 0.241174952037575 1104 68-69 0.20769278496251076 1104 70-71 0.3784421330877237 1104 72-73 0.349519030514827 1104 74-75 0.2614956271108113 1104 76-77 0.21033272855847684 1104 78-79 0.25340400292638776 1104 80-81 0.3298928972377553 1104 82-83 0.26950538738040564 1104 84-85 0.3258680981510267 1104 86-87 0.3468502903808819 1104 88-89 0.3967987161155335 1104 90-91 0.38703775349852165 1104 92-93 0.404560354738706 1104 94-95 0.540296555893022 1104 96-97 0.4799670324068188 1104 98-99 0.46524505012407147 1104 100-101 0.3602722647253813 1104 102-103 0.4029703976630792 1104 104-105 0.40631006098766065 1104 106-107 0.3595616917432096 1104 108-109 0.3499820328342622 1104 110-111 0.430926863334097 1104 112-113 0.453555395676144 1104 114-115 0.3204128553039318 1104 116-117 0.3644451869033176 1104 118-119 0.3241230666776218 1104 120-121 0.2463985743920638 1104 122-123 0.33545614976496196 1104 124-125 0.4772844500500888 1105 1 0.1500675852466955 1105 2 0.5719910725255559 1105 3 0.22685193510924861 1105 4 0.1854711664106361 1105 5 0.3285535828471211 1105 6 0.21862740241684975 1105 7 0.33974534000684997 1105 8 0.08129371271189001 1105 9 0.280334065783137 1105 10-11 0.0907926992469612 1105 12-13 0.13890662075520765 1105 14-15 0.2002948302590326 1105 16-17 0.3819454615981783 1105 18-19 0.2828813905077183 1105 20-21 0.21661721639587483 1105 22-23 0.13538495411077633 1105 24-25 0.19176545688372926 1105 26-27 0.11548525982949087 1105 28-29 0.06806372122488824 1105 30-31 0.10188012548979941 1105 32-33 0.0023907249834564936 1105 34-35 0.10561644736108633 1105 36-37 -0.021659466821951412 1105 38-39 0.03316529452798278 1105 40-41 -0.004157127479849976 1105 42-43 -0.07775608911008192 1105 44-45 -0.06472185884901194 1105 46-47 0.013957485023261995 1105 48-49 -0.04408656505007258 1105 50-51 -0.13335228028444135 1105 52-53 -0.01886125303589381 1105 54-55 -0.10127222710073625 1105 56-57 -0.1393821287686734 1105 58-59 -0.12734620160945553 1105 60-61 -0.16544060528839566 1105 62-63 -0.2793344903496404 1105 64-65 -0.25361661748038955 1105 66-67 -0.25795983849065607 1105 68-69 -0.1793746103016005 1105 70-71 -0.18357701427977702 1105 72-73 -0.22650013853712636 1105 74-75 -0.22259069867633485 1105 76-77 -0.20456286082481512 1105 78-79 -0.25428476019270363 1105 80-81 -0.219440848100092 1105 82-83 -0.21886741164813373 1105 84-85 -0.13794489517015762 1105 86-87 -0.08443223629098995 1105 88-89 -0.09570334390701873 1105 90-91 -0.19951843090602495 1105 92-93 -0.11127633968405348 1105 94-95 -0.20342254376869917 1105 96-97 -0.15480126926722093 1105 98-99 -0.16351286469941684 1105 100-101 -0.09233800069226561 1105 102-103 -0.13127422459384164 1105 104-105 -0.16097135977736698 1105 106-107 -0.16490649717169958 1105 108-109 -0.29418051378730325 1105 110-111 -0.3552211113384658 1105 112-113 -0.20987974614014604 1105 114-115 -0.25264096549579307 1105 116-117 -0.2117686000686696 1105 118-119 -0.2283836555012293 1105 120-121 -0.2216678233874454 1105 122-123 -0.16196719571166085 1105 124-125 -0.17119818070713322 1106 1 0.1514609318257385 1106 2 0.5275211555659531 1106 3 0.17649659352282043 1106 4 0.16300313441715275 1106 5 0.2557779702331331 1106 6 0.17339649406695656 1106 7 0.23843541591494244 1106 8 0.18702566543490207 1106 9 0.14050460427210254 1106 10-11 0.14156069557892437 1106 12-13 0.14365724483183584 1106 14-15 0.1162259780505579 1106 16-17 0.0709776333848211 1106 18-19 0.04966172827327142 1106 20-21 0.012804299283445175 1106 22-23 -0.03272918822964499 1106 24-25 0.08730967717003324 1106 26-27 0.12769550996959111 1106 28-29 0.08286070527909573 1106 30-31 0.0573977906790617 1106 32-33 -0.1328278928778701 1106 34-35 -0.014482003909989771 1106 36-37 -0.07624324592431009 1106 38-39 0.06708767977430341 1106 40-41 -0.17163891463151515 1106 42-43 -0.09576547573192329 1106 44-45 -0.16628873693755253 1106 46-47 0.052024885298365575 1106 48-49 5.638883607019807E-4 1106 50-51 -0.0024904177341369405 1106 52-53 -0.0844997853095748 1106 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-1.4694148275513754 2216 58-59 -1.4900355955488536 2216 60-61 -1.1669832755810674 2216 62-63 -0.9759092119243604 2216 64-65 -0.8836493162630887 2216 66-67 -1.0608541663849849 2216 68-69 -0.9531119840389763 2216 70-71 -0.8512942507470171 2216 72-73 -0.7914509284879188 2216 74-75 -1.0705559278915686 2216 76-77 -0.7995366370485968 2216 78-79 -0.8588771585351012 2216 80-81 -0.7704914179006579 2216 82-83 -0.7434335862143087 2216 84-85 -0.7482272054524692 2216 86-87 -0.7457036038123555 2216 88-89 -0.8468478013014753 2216 90-91 -0.7534212410588346 2216 92-93 -0.567646127303842 2216 94-95 -0.7112993891455446 2216 96-97 -0.8428953511113022 2216 98-99 -0.9448000959866469 2216 100-101 -1.0626905647948277 2216 102-103 -0.9409397905094075 2216 104-105 -0.8499282362342413 2216 106-107 -0.7104338864490884 2216 108-109 -0.7797412493480351 2216 110-111 -0.6724446285619834 2216 112-113 -0.6750264050368031 2216 114-115 -0.7720433223981473 2216 116-117 -0.7290973361474045 2216 118-119 -0.934509011626588 2216 120-121 -0.8561698953895167 2216 122-123 -0.8680747455692099 2216 124-125 -0.8518945676535203 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37.0 3 37.0 4 112.0 5 217.0 6 559.0 7 1137.0 8 1829.0 9 3009.0 10 4820.0 11 5879.0 12 6495.0 13 8034.0 14 10664.0 15 7751.0 16 4110.0 17 3716.0 18 3634.0 19 3387.0 20 3537.0 21 3782.0 22 4413.0 23 5436.0 24 7374.0 25 9642.0 26 12914.0 27 16830.0 28 21806.0 29 28020.0 30 35540.0 31 44655.0 32 56351.0 33 71842.0 34 96897.0 35 135712.0 36 225012.0 37 361411.0 38 143426.0 39 404.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.27095429904354 18.629312158961913 10.523757443188723 23.57597609880583 2 18.908449765555808 20.955948780682863 36.736649417814206 23.398952035947122 3 17.584547144242947 26.834119502676923 32.0335004406004 23.54783291247973 4 11.75722677756476 16.51623298966477 36.835553738848915 34.89098649392156 5 10.931795666345826 41.478478071277145 35.44095668494647 12.148769577430558 6 31.5671811443902 37.262548030961966 17.88762254420996 13.282648280437876 7 26.96235498148369 34.23247837171984 22.335239638308067 16.4699270084884 8 27.20027902203074 33.164819231786005 20.284338851818422 19.35056289436484 9 27.06824710037018 11.509436616902308 19.403731105106445 42.01858517762107 10-11 24.224155103074498 25.64070285708785 30.019490073909736 20.115651965927913 12-13 25.3906345455636 24.853324605255654 29.443229605955434 20.312811243225312 14-15 22.776246990775537 26.285756177101977 26.647492541270157 24.290504290852326 16-17 22.070385755050523 28.63814808547947 27.19953170454289 22.091934454927117 18-19 21.366993204391783 26.990642246808612 29.729323453030922 21.913041095768683 20-21 22.668688737151324 26.421157393454386 29.521871165576023 21.388282703818263 22-23 22.331685217534254 26.80255414752771 29.15365538853892 21.712105246399112 24-25 21.937189659149432 26.841884865603973 29.325204073812017 21.895721401434578 26-27 21.865019390105825 27.002823543002197 29.1555806997914 21.976576367100577 28-29 21.840249520338322 26.837246775288776 29.41001798685012 21.912485717522774 30-31 22.02415377016671 26.42611877245117 29.560488466274844 21.989238991107282 32-33 21.655826917480418 26.84513314638067 29.34933365718056 22.149706278958348 34-35 22.220955134224113 26.884735802278982 29.08155784684559 21.812751216651314 36-37 22.27873915809101 26.529826403570418 28.926061383365752 22.26537305497282 38-39 22.014934491284635 26.667412107690065 29.054316732954145 22.263336668071158 40-41 22.128499023089304 26.788050181034862 29.003973177442305 22.07947761843353 42-43 22.247087503739664 26.592801476316435 28.856706368836214 22.30340465110768 44-45 22.041759732680834 26.944536605307277 28.656568633840283 22.3571350281716 46-47 22.468051165532586 27.12592313913221 27.88613358374979 22.519892111585417 48-49 22.488450731762228 27.157517355376797 27.753161885267147 22.600870027593828 50-51 22.558538062885926 27.130942489102566 27.777827148864194 22.53269229914731 52-53 22.680178370486445 27.24885778386186 27.726405771010242 22.344558074641448 54-55 22.143130093222073 27.287562096208024 27.858943483193666 22.710364327376244 56-57 22.50780534910794 27.392258041583105 27.606460814777734 22.49347579453122 58-59 22.694525749471904 26.669888011967114 27.826202866997317 22.809383371563666 60-61 22.610695078264573 26.981440512445502 28.08619748035286 22.321666928937063 62-63 22.295077232728993 27.172043225742907 28.049968935107326 22.48291060642077 64-65 22.029538002186026 27.1229328285925 28.164442134011058 22.683087035210413 66-67 22.23300100933879 27.5043709627374 27.67909839312968 22.58352963479412 68-69 22.504351470724902 27.03243581279845 27.794540232544968 22.66867248393168 70-71 22.228755333660153 27.00624117844824 27.92942166165822 22.83558182623339 72-73 22.311727627104585 27.179861432007492 27.696307124491998 22.81210381639593 74-75 22.120154870852712 27.328255636876207 27.69418005051038 22.857409441760705 76-77 22.104719466387245 27.228949565597304 27.96668437723889 22.699646590776556 78-79 22.39518404000514 27.18815129886743 27.684550029194483 22.732114631932948 80-81 22.150738527033536 27.330035851689598 27.47821148090841 23.041014140368453 82-83 22.422093160163413 27.2078016805055 27.484751119460732 22.88535403987035 84-85 22.174813243840454 27.099266600712074 27.617066936014172 23.108853219433303 86-87 22.113425116852188 27.122296404684747 27.60751438066859 23.156764097794472 88-89 22.142373955732655 27.016935986407077 27.62382641993145 23.21686363792882 90-91 22.088179733279226 27.134273449145844 27.663819857953303 23.113726959621623 92-93 22.13254207127949 26.85019769826568 27.795831878535253 23.221428351919574 94-95 21.8396277587363 26.77203925165199 27.84556149241585 23.54277149719586 96-97 22.13528534032383 26.741236888192798 27.70257811545425 23.420899656029118 98-99 21.935131288439873 26.778976225271762 27.56207491903352 23.723817567254848 100-101 21.931664482762834 26.662707376756916 27.680879258566193 23.724748881914053 102-103 21.90278789817596 26.350925053761532 27.713994585340135 24.032292462722378 104-105 21.755954551799388 26.260416693664514 27.852666195947695 24.130962558588408 106-107 21.86533750043042 26.564281273869877 27.64264908596989 23.92773213972981 108-109 21.95857409336861 26.529034493942955 27.26466238796929 24.247729024719142 110-111 21.803909400949266 26.46567361133995 27.58899787213009 24.141419115580696 112-113 21.777620278588415 26.414946919934252 27.565085984559403 24.24234681691793 114-115 21.67199997926174 26.42817174093618 27.481267981645157 24.418560298156926 116-117 21.537989810330536 26.47588378236857 27.540029521749144 24.44609688555175 118-119 21.521621119222203 26.561772140086816 27.19249457703864 24.724112163652347 120-121 21.347171324565913 26.446885063434 27.413382032161515 24.79256157983857 122-123 21.559748017804488 26.36259857287107 27.25037925816104 24.827274151163397 124-125 21.511975576747552 26.275879816566416 27.005356008893973 25.206788597792055 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2.0 1 1.0 2 2.5 3 7.5 4 12.5 5 17.0 6 22.5 7 47.0 8 88.5 9 146.0 10 222.0 11 306.0 12 387.0 13 496.0 14 627.0 15 773.0 16 923.0 17 1091.5 18 1262.5 19 1414.0 20 1616.0 21 1897.0 22 2318.0 23 3230.0 24 4422.5 25 5474.5 26 7244.0 27 9141.0 28 10450.5 29 12821.5 30 16649.0 31 20237.5 32 23417.0 33 26061.5 34 29217.0 35 31814.0 36 34702.5 37 39618.5 38 42217.5 39 42677.5 40 43172.0 41 45204.0 42 46918.5 43 47950.5 44 50218.0 45 51593.0 46 51873.5 47 52097.0 48 52384.5 49 51872.0 50 50176.5 51 47919.0 52 45832.5 53 43762.5 54 41728.5 55 38689.5 56 33856.5 57 30240.5 58 27955.5 59 25302.0 60 21829.0 61 17556.5 62 14087.5 63 10873.0 64 7952.0 65 6185.0 66 4790.0 67 3769.0 68 2893.5 69 1998.5 70 1448.5 71 1167.5 72 1075.5 73 923.5 74 682.5 75 538.0 76 362.0 77 223.0 78 139.0 79 63.5 80 27.5 81 10.0 82 8.0 83 4.5 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06479412868928512 2 2.221512983632633E-4 3 7.405043278775443E-5 4 1.4810086557550886E-4 5 0.06323906960074228 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.1107564918163164E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 3.7025216393877215E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 2.221512983632633E-4 36-37 0.0 38-39 0.0 40-41 3.7025216393877215E-5 42-43 0.00318416860987344 44-45 0.009996808426346848 46-47 0.0108854136197999 48-49 0.009552505829620321 50-51 0.008367698905016251 52-53 0.007071816331230547 54-55 0.007923396308289723 56-57 0.005553782459081582 58-59 0.004702202482022406 60-61 0.003221193826267318 62-63 0.0023696138492081418 64-65 0.002591765147571405 66-67 0.003110118177085686 68-69 0.001888286036087738 70-71 0.003332269475448949 72-73 0.0034803703410244582 74-75 0.0011477817082101937 76-77 9.996808426346849E-4 78-79 3.332269475448949E-4 80-81 4.8132781312040383E-4 82-83 2.962017311510177E-4 84-85 5.183530295142809E-4 86-87 5.183530295142809E-4 88-89 0.0016661347377244745 90-91 0.002221512983632633 92-93 0.0010737312754224393 94-95 0.001221832140997948 96-97 0.0019253112524816153 98-99 0.0019623364688754925 100-101 0.0015180338721489657 102-103 0.0017031599541183517 104-105 0.0010737312754224393 106-107 7.775295442714216E-4 108-109 0.0014810086557550885 110-111 0.0014439834393612115 112-113 0.011811044029646832 114-115 0.01995659163629982 116-117 0.017772103869061064 118-119 0.011403766649314183 120-121 0.006701564167291776 122-123 0.007219917196806057 124-125 0.005812958973838722 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1350431.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.29484689149075 #Duplication Level Percentage of deduplicated Percentage of total 1 41.261645069606544 12.087535748051579 2 18.62511745954197 10.912399286266263 3 13.171616034791327 11.575814251581493 4 8.80721043465746 10.3202352489772 5 5.604873687223868 8.20969582566842 6 3.577104257101924 6.287443291602038 7 2.2743594302319328 4.663890789940383 8 1.4718405403284618 3.4493874622089042 9 1.0022643262293174 2.6425061983521894 >10 3.870204157489248 19.19985499941751 >50 0.2292239022595604 4.650231051671886 >100 0.1007066953109426 5.066531766549217 >500 0.0028116038334635683 0.4857318393507391 >1k 0.0010224013939867521 0.4487422403621057 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2107 0.15602426188379856 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1355 0.10033833642740725 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.7025216393877215E-5 0.0 16-17 0.0 0.0 0.0 7.405043278775443E-5 0.0 18-19 0.0 0.0 0.0 7.405043278775443E-5 0.0 20-21 0.0 0.0 0.0 7.405043278775443E-5 0.0 22-23 0.0 0.0 0.0 7.405043278775443E-5 0.0 24-25 0.0 0.0 0.0 7.405043278775443E-5 0.0 26-27 0.0 0.0 0.0 1.1107564918163164E-4 0.0 28-29 0.0 0.0 0.0 3.702521639387721E-4 0.0 30-31 0.0 0.0 0.0 0.0016291095213305975 0.0 32-33 0.0 0.0 0.0 0.005405681593506073 0.0 34-35 0.0 0.0 0.0 0.010996489268981533 0.0 36-37 0.0 0.0 0.0 0.02021576815105696 0.0 38-39 0.0 0.0 0.0 0.03909862851193434 0.0 40-41 0.0 0.0 0.0 0.061165657482685154 0.0 42-43 0.0 0.0 0.0 0.08819406545021552 0.0 44-45 0.0 0.0 0.0 0.12384934883751927 0.0 46-47 0.0 0.0 0.0 0.1714267519036515 0.0 48-49 0.0 0.0 0.0 0.2200038358124184 0.0 50-51 0.0 0.0 0.0 0.2647302972162221 0.0 52-53 0.0 0.0 0.0 0.31649154973486243 0.0 54-55 0.0 0.0 0.0 0.38309991402744753 0.0 56-57 0.0 0.0 0.0 0.4660734239661264 0.0 58-59 0.0 0.0 0.0 0.5639310708951438 0.0 60-61 0.0 0.0 0.0 0.6640472560241878 0.0 62-63 0.0 0.0 0.0 0.7635710376909297 0.0 64-65 0.0 0.0 0.0 0.8814593266890349 0.0 66-67 0.0 0.0 0.0 1.024932040215309 0.0 68-69 0.0 0.0 0.0 1.196803094715687 0.0 70-71 0.0 0.0 0.0 1.3791522854555325 0.0 72-73 0.0 0.0 0.0 1.5625011570380123 0.0 74-75 0.0 0.0 0.0 1.7729154618044165 0.0 76-77 0.0 0.0 0.0 2.016208158728584 0.0 78-79 0.0 0.0 0.0 2.3054121239811587 0.0 80-81 0.0 0.0 0.0 2.599873669961664 0.0 82-83 0.0 0.0 0.0 2.9021105113848837 0.0 84-85 0.0 0.0 0.0 3.2295615251723335 0.0 86-87 0.0 0.0 0.0 3.596925722232384 0.0 88-89 0.0 0.0 0.0 4.011867322358565 0.0 90-91 0.0 0.0 0.0 4.4551332130260635 0.0 92-93 0.0 0.0 0.0 4.902657003578858 0.0 94-95 0.0 0.0 0.0 5.386909808794377 0.0 96-97 0.0 0.0 0.0 5.902152720131573 0.0 98-99 0.0 0.0 0.0 6.4735999099546735 0.0 100-101 0.0 0.0 0.0 7.073667592050242 0.0 102-103 0.0 0.0 0.0 7.669995727290028 1.4810086557550886E-4 104-105 0.0 0.0 0.0 8.301534843320391 1.4810086557550886E-4 106-107 0.0 0.0 0.0 8.975912134718472 1.4810086557550886E-4 108-109 0.0 0.0 0.0 9.69697822398923 2.962017311510177E-4 110-111 0.0 0.0 0.0 10.436705022322503 4.443025967265266E-4 112-113 0.0 0.0 0.0 11.186354578649336 4.443025967265266E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2930 0.0 80.708336 1 GTATCAA 4235 0.0 55.520004 2 GTACATG 9730 0.0 47.26068 1 TACATGG 9785 0.0 46.659523 2 ACATGGG 9880 0.0 45.246895 3 TCAACGC 6025 0.0 40.70483 5 ATCAACG 6065 0.0 40.436375 4 CAACGCA 6155 0.0 40.017773 6 CATGGGA 6710 0.0 39.920483 4 AACGCAG 6295 0.0 39.316803 7 CATGGGG 3150 0.0 36.66038 4 TATCAAC 6835 0.0 35.968086 3 ATGGGAT 2930 0.0 33.927715 5 ATGGGAG 2510 0.0 32.964527 5 ACGCAGA 7560 0.0 32.344517 8 CGCAGAG 7685 0.0 31.663582 9 ATGGGCG 210 9.094947E-11 31.180222 5 ATGGGGG 1675 0.0 28.785646 5 CAGAGTA 8475 0.0 28.501448 9 TATTACG 85 0.00719552 28.012177 2 >>END_MODULE