FastQCFastQC Report
Thu 26 May 2016
SRR936276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512475
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23700.074.053981
GTATCAA29350.059.5955662
GTACATG128150.052.878591
TACATGG129600.051.3688472
ACATGGG129550.050.4242743
CATGGGA90300.047.3054774
TCAACGC39150.045.437475
ATCAACG39350.045.206534
CAACGCA39650.045.014546
AACGCAG41650.042.7101337
CATGGGG37750.040.3457454
TATCAAC45000.039.5305983
ATGGGAT40300.039.1215065
ATGGGAG32200.038.8005755
ACGCAGA49850.034.9685138
CGCAGAG50950.034.0967839
ATGGGAC17500.030.936995
TACCTGG13150.030.3126412
GAGTACA76100.029.9425541
AGTACAT75550.029.2943042
Produced by FastQC (version 0.11.5)