##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936276_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1512475 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.96829038496504 31.0 30.0 33.0 26.0 34.0 2 30.091620687945255 31.0 30.0 34.0 26.0 34.0 3 30.293718573860726 31.0 31.0 34.0 26.0 34.0 4 33.79634572472272 37.0 35.0 37.0 28.0 37.0 5 34.0756977801286 37.0 35.0 37.0 30.0 37.0 6 34.07828426916147 37.0 35.0 37.0 30.0 37.0 7 33.922831121175555 37.0 35.0 37.0 30.0 37.0 8 33.948089059323294 37.0 35.0 37.0 30.0 37.0 9 35.34766062248963 39.0 35.0 39.0 29.0 39.0 10-11 35.18179738508074 39.0 35.0 39.0 28.0 39.0 12-13 35.211665316782096 39.0 35.0 39.0 28.0 39.0 14-15 36.38725532653432 40.0 36.0 41.0 28.5 41.0 16-17 36.4227428552538 40.0 36.0 41.0 28.5 41.0 18-19 36.46735714639911 40.0 36.0 41.0 28.5 41.0 20-21 36.57272252433924 40.0 36.0 41.0 29.0 41.0 22-23 36.47018859815864 40.0 36.0 41.0 28.5 41.0 24-25 36.46223739235359 40.0 36.0 41.0 28.5 41.0 26-27 36.56004429825286 40.0 36.0 41.0 30.0 41.0 28-29 36.46202813269641 40.0 36.0 41.0 29.5 41.0 30-31 36.40040827121109 40.0 36.0 41.0 29.5 41.0 32-33 36.21988032860047 39.0 35.0 41.0 29.0 41.0 34-35 36.187613348980975 39.0 35.0 41.0 29.0 41.0 36-37 36.09260781169937 39.0 35.0 41.0 28.5 41.0 38-39 35.86315079588093 39.0 35.0 41.0 27.5 41.0 40-41 35.96288765103556 39.0 35.0 41.0 28.5 41.0 42-43 35.894350981007946 39.0 35.0 41.0 27.5 41.0 44-45 35.79953222367312 39.0 35.0 41.0 27.5 41.0 46-47 35.78430949271889 39.0 35.0 41.0 28.0 41.0 48-49 35.69595133803864 39.0 35.0 41.0 28.0 41.0 50-51 35.56950594224698 39.0 34.5 41.0 28.0 41.0 52-53 35.424821236714656 39.0 34.0 40.5 27.5 41.0 54-55 35.25581315393643 39.0 34.0 40.0 27.0 41.0 56-57 35.06663250632242 38.0 34.0 40.0 27.0 41.0 58-59 34.847284087340284 38.0 34.0 40.0 26.5 41.0 60-61 34.6063445676788 38.0 33.0 40.0 26.0 41.0 62-63 34.36889270897039 37.5 33.0 40.0 26.0 41.0 64-65 34.08830261657218 37.0 33.0 40.0 26.0 41.0 66-67 33.78706457957983 36.5 33.0 39.5 25.5 41.0 68-69 33.499465776293825 36.0 32.0 39.0 25.0 41.0 70-71 33.155979768260636 36.0 32.0 39.0 24.5 40.5 72-73 32.82236070017686 35.0 32.0 38.5 24.0 40.0 74-75 32.46843518074679 35.0 32.0 37.5 23.0 40.0 76-77 32.13024744210648 35.0 31.5 37.0 23.0 39.0 78-79 31.783486669201146 35.0 31.0 36.5 22.0 39.0 80-81 31.469586935321246 35.0 31.0 36.0 21.5 38.5 82-83 31.16234251805815 35.0 31.0 36.0 20.0 37.0 84-85 31.180724640076697 35.0 31.0 35.5 21.5 37.0 86-87 31.05733813782046 35.0 31.0 35.0 21.0 37.0 88-89 30.88537529545943 35.0 31.0 35.0 20.0 36.0 90-91 30.717361609282797 35.0 31.0 35.0 20.0 36.0 92-93 30.60362551447131 35.0 31.0 35.0 20.0 36.0 94-95 30.476251508289394 34.0 31.0 35.0 19.0 35.5 96-97 30.380041984165025 34.0 31.0 35.0 18.5 35.0 98-99 30.279871402833102 34.0 31.0 35.0 18.0 35.0 100-101 30.190171407791865 34.0 31.0 35.0 17.5 35.0 102-103 29.220561331592258 33.5 29.5 34.5 14.0 35.0 104-105 29.90474685531992 34.0 30.5 35.0 15.0 35.0 106-107 30.03561381179854 34.0 31.0 35.0 14.0 35.0 108-109 29.99767202763682 34.0 31.0 35.0 10.0 35.0 110-111 29.89392981702177 34.0 31.0 35.0 7.0 35.0 112-113 29.86268235838609 34.0 31.0 35.0 6.0 35.0 114-115 29.769504619911075 34.0 31.0 35.0 2.0 35.0 116-117 29.66610158845601 34.0 31.0 35.0 2.0 35.0 118-119 29.47485776624407 34.0 30.0 35.0 2.0 35.0 120-121 29.304340567612687 34.0 30.0 35.0 2.0 35.0 122-123 29.025394799914046 34.0 30.0 35.0 2.0 35.0 124-125 27.008064596109026 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3625720802790866 1101 2 0.4968411838719078 1101 3 0.3032289298386317 1101 4 0.3625411242909493 1101 5 0.34421226598689003 1101 6 0.339109217180507 1101 7 0.29264017216644334 1101 8 0.43977680943947917 1101 9 0.4822408701892371 1101 10-11 0.5475185094506116 1101 12-13 0.3956399894921674 1101 14-15 0.4611267561145951 1101 16-17 0.5041463849400785 1101 18-19 0.4700280184093799 1101 20-21 0.3611680983895198 1101 22-23 0.5944679902657413 1101 24-25 0.6536820732286586 1101 26-27 0.48729618209618764 1101 28-29 0.595476149935962 1101 30-31 0.5840298686254073 1101 32-33 0.705547602799129 1101 34-35 0.5466149883954969 1101 36-37 0.5720854119487129 1101 38-39 0.6380742235938115 1101 40-41 0.6464523036327421 1101 42-43 0.7404848797447698 1101 44-45 0.6293018802490522 1101 46-47 0.5479164988967895 1101 48-49 0.5884457304145556 1101 50-51 0.5957712151362315 1101 52-53 0.5232958631655151 1101 54-55 0.6436341903054199 1101 56-57 0.5835663012291121 1101 58-59 0.5422624303148567 1101 60-61 0.643994769293009 1101 62-63 0.5558504812304079 1101 64-65 0.6092830899531094 1101 66-67 0.47550560973637346 1101 68-69 0.46413271484730245 1101 70-71 0.6032222678218133 1101 72-73 0.5785611192417832 1101 74-75 0.5859798020193452 1101 76-77 0.5271613657025611 1101 78-79 0.500177613362979 1101 80-81 0.5535814547611544 1101 82-83 0.5955626115857413 1101 84-85 0.3569208323144828 1101 86-87 0.5032891841748253 1101 88-89 0.5422457287481599 1101 90-91 0.5597204452377262 1101 92-93 0.5500471526042254 1101 94-95 0.7040225721947913 1101 96-97 0.6182511978499825 1101 98-99 0.759466598961513 1101 100-101 0.7407231671831944 1101 102-103 0.7098114328224625 1101 104-105 0.8007101791089326 1101 106-107 0.7749292940097341 1101 108-109 0.6381330974649053 1101 110-111 0.6547636370421337 1101 112-113 0.7022033146199789 1101 114-115 0.7092888019204793 1101 116-117 0.7337423648524606 1101 118-119 0.7537365438709003 1101 120-121 0.8487910251520923 1101 122-123 0.8027337081125268 1101 124-125 0.5730799482738611 1102 1 0.38272069035694756 1102 2 0.33716982139550566 1102 3 0.5291035034071427 1102 4 0.6078351878903732 1102 5 0.4528348515177072 1102 6 0.4737148551160857 1102 7 0.5602020362240836 1102 8 0.5273586502008456 1102 9 0.7181601097703805 1102 10-11 0.8232733413528734 1102 12-13 0.71596450719111 1102 14-15 0.7654528683931545 1102 16-17 0.7925430025343942 1102 18-19 0.6582202749173689 1102 20-21 0.695411843814 1102 22-23 0.8647907296231097 1102 24-25 0.8769387797993389 1102 26-27 0.7658563478376195 1102 28-29 0.7932499626706715 1102 30-31 0.7662261501131695 1102 32-33 0.8810321267815482 1102 34-35 0.7177196052666162 1102 36-37 0.7027031188957977 1102 38-39 0.8075553627994267 1102 40-41 0.8423699723454945 1102 42-43 0.9376510924403547 1102 44-45 0.8933055791995343 1102 46-47 0.8285647340409668 1102 48-49 0.7813157662481345 1102 50-51 0.7778141146628954 1102 52-53 0.8187273036518263 1102 54-55 0.7977934348659161 1102 56-57 0.8353802096386005 1102 58-59 0.8429881418737821 1102 60-61 0.8577862528151954 1102 62-63 0.8796835186674627 1102 64-65 0.8168651974657166 1102 66-67 0.8512417690520664 1102 68-69 0.7681668827168195 1102 70-71 0.7784400405675456 1102 72-73 0.6543095787533915 1102 74-75 0.5924129526774777 1102 76-77 0.5324763840935773 1102 78-79 0.6358917508821378 1102 80-81 0.6228285938541411 1102 82-83 0.6553934320236294 1102 84-85 0.6528502084426187 1102 86-87 0.5571158788602517 1102 88-89 0.6017734901421434 1102 90-91 0.6494822363833634 1102 92-93 0.5333781642138327 1102 94-95 0.5302408102732343 1102 96-97 0.6782139119548987 1102 98-99 0.6088877487778994 1102 100-101 0.7665113429900465 1102 102-103 0.7665672042818592 1102 104-105 0.6869511503205459 1102 106-107 0.6432179069922164 1102 108-109 0.6850049946293417 1102 110-111 0.7604037955516425 1102 112-113 0.650494328363596 1102 114-115 0.6645820356271663 1102 116-117 0.5938876553593779 1102 118-119 0.8016013484163409 1102 120-121 0.7019074205722688 1102 122-123 0.7551363957794344 1102 124-125 0.6075271656138774 1103 1 0.059702626191356245 1103 2 0.08707367447319214 1103 3 0.2869540018982484 1103 4 0.1246789012727163 1103 5 0.31858674499837036 1103 6 0.20136090024540465 1103 7 0.09718955267134533 1103 8 0.26089216006265303 1103 9 0.11312552960921352 1103 10-11 0.2715741183836613 1103 12-13 0.01510663116253852 1103 14-15 0.07244630912737193 1103 16-17 0.11495227257059781 1103 18-19 0.13541299753636338 1103 20-21 0.015432169682519259 1103 22-23 0.262694972198112 1103 24-25 0.2556987381656697 1103 26-27 0.13042993010010662 1103 28-29 0.2041653386501423 1103 30-31 0.1286411316856899 1103 32-33 0.20663613402162184 1103 34-35 0.15296956271649265 1103 36-37 0.012795559047411587 1103 38-39 0.1828131183783981 1103 40-41 0.220978141315328 1103 42-43 0.299189091340601 1103 44-45 0.32826320909198614 1103 46-47 0.23149234645512706 1103 48-49 0.2448895841728742 1103 50-51 0.24142045888687136 1103 52-53 0.24444889653221935 1103 54-55 0.20765426321869995 1103 56-57 0.23284708600101567 1103 58-59 0.1885802759917823 1103 60-61 0.06901060818076132 1103 62-63 0.13579355329672183 1103 64-65 0.18925859303134018 1103 66-67 0.16915013571632898 1103 68-69 0.15801974110345185 1103 70-71 0.22273619599988592 1103 72-73 0.22442932463093257 1103 74-75 0.13988621155900205 1103 76-77 0.09823371908090195 1103 78-79 0.13149988221146458 1103 80-81 0.08362376572392449 1103 82-83 -0.07061975196966941 1103 84-85 0.09058198389577754 1103 86-87 0.0815938624424497 1103 88-89 0.10738990238086998 1103 90-91 0.09120656392769888 1103 92-93 0.13516788803077517 1103 94-95 0.20099508492448948 1103 96-97 0.14847669172198508 1103 98-99 0.04228186605914175 1103 100-101 0.100496702473162 1103 102-103 0.019151919948818374 1103 104-105 0.07291987761350427 1103 106-107 0.04552445120180337 1103 108-109 0.027786160961060347 1103 110-111 0.019860356781276067 1103 112-113 0.05469169385609618 1103 114-115 -0.04007262099331044 1103 116-117 -0.06421818480925268 1103 118-119 -0.03919939588357835 1103 120-121 -0.08431132963229615 1103 122-123 -0.06952639446152986 1103 124-125 0.12465666038021084 1104 1 -0.1329382676927473 1104 2 -0.03957790791246296 1104 3 -0.08644248548729294 1104 4 -0.08833843233070127 1104 5 -0.20142729216608757 1104 6 -0.04913938144741081 1104 7 -0.3244215086775526 1104 8 -0.19785388753393818 1104 9 -0.04541510378816582 1104 10-11 -0.048870054214908976 1104 12-13 -0.09588350325873307 1104 14-15 -0.1394084481578659 1104 16-17 -0.06214131432847125 1104 18-19 -0.08080226166850935 1104 20-21 -0.07998850213902386 1104 22-23 -0.04728027681517233 1104 24-25 -0.01037278648878015 1104 26-27 -0.02761596050812898 1104 28-29 0.0853272700816774 1104 30-31 -0.007398694614295209 1104 32-33 0.0817622429892566 1104 34-35 0.06976523968130266 1104 36-37 0.04799262137115079 1104 38-39 0.15326069344637006 1104 40-41 0.06786563352129349 1104 42-43 0.1447653470100576 1104 44-45 0.13733977098368655 1104 46-47 0.04929683581568867 1104 48-49 -0.11135212725341859 1104 50-51 -0.015951525180071258 1104 52-53 0.04692219506013373 1104 54-55 -0.002330322803636875 1104 56-57 0.08070111689860227 1104 58-59 -0.07436493317305093 1104 60-61 -0.10406994409540715 1104 62-63 -0.10865527831110455 1104 64-65 -0.11459240887721478 1104 66-67 -0.10982940832111154 1104 68-69 -0.10803101266220239 1104 70-71 -0.024834203465850635 1104 72-73 0.019126892577460808 1104 74-75 -0.10340518699835144 1104 76-77 -0.17290580352890217 1104 78-79 -0.28914909135055566 1104 80-81 -0.1672635468633139 1104 82-83 -0.16822156493196871 1104 84-85 -0.17536461869896058 1104 86-87 -0.17556665419483153 1104 88-89 -0.17367039016819774 1104 90-91 -0.2181847263836545 1104 92-93 -0.1958085099093836 1104 94-95 -0.08907265191112046 1104 96-97 -0.1795844625222358 1104 98-99 -0.16859814308796217 1104 100-101 -0.17510668195524204 1104 102-103 -0.1453480792599109 1104 104-105 -0.22861141165336818 1104 106-107 -0.2222981582864989 1104 108-109 -0.20431615762401734 1104 110-111 -0.13178622080926772 1104 112-113 -0.24576069610171558 1104 114-115 -0.1986941619479623 1104 116-117 -0.22691091887540793 1104 118-119 -0.2919223604947305 1104 120-121 -0.1666378353436997 1104 122-123 -0.06177370967579421 1104 124-125 -0.09281464414238272 1105 1 0.15568799475924422 1105 2 0.3267836711817047 1105 3 0.24746402525211408 1105 4 0.3127063224730122 1105 5 0.29352405005629123 1105 6 0.15959764418359157 1105 7 0.11944860817204273 1105 8 0.09725503055057771 1105 9 0.1495510950392216 1105 10-11 0.26606754325160864 1105 12-13 0.28009777550197157 1105 14-15 0.34046679469970087 1105 16-17 0.2662937484095309 1105 18-19 0.20003913975983778 1105 20-21 0.1289017861462085 1105 22-23 0.09167335811682875 1105 24-25 0.20260709383408226 1105 26-27 0.31883489240477303 1105 28-29 0.31516134576601473 1105 30-31 0.36851472300870824 1105 32-33 0.46113772882421245 1105 34-35 0.444434919842557 1105 36-37 0.40997849896997707 1105 38-39 0.46795598447767617 1105 40-41 0.18872296506417285 1105 42-43 0.44779638197866944 1105 44-45 0.4346586809497879 1105 46-47 0.42087691722988296 1105 48-49 0.42378019717266113 1105 50-51 0.39340602729489405 1105 52-53 0.37451023961460805 1105 54-55 0.4366397023184021 1105 56-57 0.29516291960864294 1105 58-59 0.32521371433913515 1105 60-61 0.2476975605796241 1105 62-63 0.3032482170167299 1105 64-65 0.49816538916306286 1105 66-67 0.5057287159893704 1105 68-69 0.4010674597912498 1105 70-71 0.32943084028445924 1105 72-73 0.3118392006516899 1105 74-75 0.26505098170366637 1105 76-77 0.36820117648752415 1105 78-79 0.28020297855791654 1105 80-81 0.28935986312051654 1105 82-83 0.2869608502152481 1105 84-85 0.24597071212887656 1105 86-87 0.2817109256546253 1105 88-89 0.26814884424698704 1105 90-91 0.3351753104990678 1105 92-93 0.3370800837643877 1105 94-95 0.3838771126356484 1105 96-97 0.37353566415188766 1105 98-99 0.36714560252095296 1105 100-101 0.35944738506433893 1105 102-103 0.40705816358136104 1105 104-105 0.47742813375369053 1105 106-107 0.4935746385029418 1105 108-109 0.46632719088222174 1105 110-111 0.5729066452407032 1105 112-113 0.4789288727404397 1105 114-115 0.4442160265200563 1105 116-117 0.38022322284893306 1105 118-119 0.43492189531875525 1105 120-121 0.5490433591656831 1105 122-123 0.5406256799573654 1105 124-125 0.581455632966847 1106 1 -0.169300605331955 1106 2 -0.21988502213541494 1106 3 -0.1314182770227994 1106 4 -0.04344202834018063 1106 5 -0.07890843715048135 1106 6 -0.05220252808170045 1106 7 -0.07707568630026884 1106 8 -0.21949523544729743 1106 9 -0.2347322306941777 1106 10-11 -0.05303363727227861 1106 12-13 -0.11304201271305914 1106 14-15 -0.1870677850236575 1106 16-17 -0.10794499101388766 1106 18-19 -0.21080344016618824 1106 20-21 -0.16169202243666092 1106 22-23 -0.20829877543943098 1106 24-25 -4.0147841003697025 1106 26-27 -1.030197382003692 1106 28-29 -0.5306485544214468 1106 30-31 -0.3730544111048886 1106 32-33 -5.43835814187549 1106 34-35 -1.179393422864031 1106 36-37 -0.3407764283239345 1106 38-39 0.015782401866339058 1106 40-41 -0.3828329958214809 1106 42-43 -0.00895489734443089 1106 44-45 -0.05995296215706958 1106 46-47 -0.18485597024034917 1106 48-49 0.07995641442959567 1106 50-51 0.060751621863467165 1106 52-53 0.1619956730644745 1106 54-55 -0.05228971957955508 1106 56-57 0.0977671654413399 1106 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2216 98-99 -0.9156590891490239 2216 100-101 -0.8843440197797641 2216 102-103 -0.9939915059729856 2216 104-105 -0.9251564247228572 2216 106-107 -0.9405709098591863 2216 108-109 -0.9514294745392746 2216 110-111 -0.8480048977933698 2216 112-113 -0.8911415467229027 2216 114-115 -0.9737216246153118 2216 116-117 -0.9188106211623186 2216 118-119 -0.9058981555507621 2216 120-121 -1.0197921222824888 2216 122-123 -0.9641444277378604 2216 124-125 -1.0600444229467918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 26451.0 3 4490.0 4 3032.0 5 2584.0 6 3028.0 7 3593.0 8 4061.0 9 4517.0 10 5471.0 11 6297.0 12 6887.0 13 7051.0 14 7380.0 15 7045.0 16 6373.0 17 5919.0 18 5277.0 19 4957.0 20 4937.0 21 4977.0 22 5860.0 23 8071.0 24 12244.0 25 18600.0 26 22171.0 27 20702.0 28 23347.0 29 29390.0 30 35554.0 31 43090.0 32 56409.0 33 75241.0 34 105710.0 35 152978.0 36 257189.0 37 380764.0 38 140442.0 39 386.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.28086618921178 17.846110717346 9.518772017052967 22.354251076389257 2 19.66517043963157 20.089376920284753 37.78188143772674 22.46357120235694 3 16.858736084058304 28.15327734559404 30.835042453622343 24.152944116725312 4 10.864780342024444 15.700793076203418 37.23405169706406 36.200374884708076 5 11.075774866541396 42.46178726757971 34.85977094179393 11.602666924084964 6 31.021728896576168 37.45054401582591 18.847688792269 12.680038295328922 7 28.578994311904403 34.4074575343299 21.427055446865598 15.586492706900096 8 27.488073946490616 33.82509777215039 19.65730985258968 19.02951842876931 9 27.934056096220868 13.01449250448118 18.689109033963067 40.36234236533488 10-11 24.115402925074715 26.78443442384491 29.592116050884666 19.508046600195712 12-13 24.761253965876847 26.024152466868795 28.690583751285594 20.524009815968768 14-15 22.71060006526006 27.406646542059843 25.517443842131488 24.365309550548613 16-17 22.541985691805184 29.633798812499172 26.08508218616522 21.73913330953042 18-19 21.68024699480072 28.53012769927411 27.857728720286456 21.931896585638714 20-21 23.46952471756922 27.742583671315774 27.184267980952203 21.6036236301628 22-23 22.996128300465855 28.130224900047544 26.82845237571335 22.04519442377325 24-25 22.395649095060172 28.063845979393303 27.324633815584416 22.21587110996211 26-27 22.37619389388837 28.170655872597543 27.151802506619287 22.3013477268948 28-29 22.28570314005741 28.008837372445395 27.433145303897906 22.27231418359929 30-31 22.527187711577522 27.694340565758296 27.49654864167231 22.281923080991874 32-33 22.139577660909502 28.2163717557262 27.293686865013612 22.350363718350685 34-35 22.568583994731732 28.03526987963916 27.22053327973384 22.175612845895273 36-37 22.680654006081472 27.743356366403678 27.066645597280726 22.50934403023413 38-39 22.31263105800818 27.792413702134382 27.465526970965332 22.429428268892107 40-41 22.40215174908955 27.82612116547786 27.557516179111424 22.21421090632116 42-43 22.336290687030168 27.763068330771805 27.473966813327692 22.426674168870328 44-45 22.267678405602055 27.893864605369252 27.368523361668238 22.469933627360458 46-47 22.41083657562465 27.950980985279685 27.195807286733597 22.44237515236207 48-49 22.337229398875742 27.83560093251683 27.3494081850435 22.477761483563928 50-51 22.387348684430194 27.75769805615879 27.354079673058862 22.500873586352153 52-53 22.530810221465977 27.83361637402232 27.28919970286513 22.346373701646574 54-55 22.273225113169666 27.850796212613844 27.411374258111803 22.464604416104688 56-57 22.36293629286893 27.98800220437841 27.306125943794964 22.3429355589577 58-59 22.57327410510531 27.478347282513294 27.32505341483 22.6233251975514 60-61 22.533353917988222 27.79662316891065 27.56666568592026 22.10335722718087 62-63 22.167979662070355 27.869509304470576 27.466585561789024 22.495925471670045 64-65 22.18928084281739 27.738882119156255 27.436723569887555 22.635113468138805 66-67 22.208227421896616 28.034207699806906 27.284736554495588 22.472828323800893 68-69 22.266572075196255 27.8045698787876 27.387834064698186 22.541023981317963 70-71 22.174959279928302 27.714442790406757 27.491924582493226 22.61867334717171 72-73 22.412194894573044 27.75941192844283 27.314675540457355 22.513717636526763 74-75 22.089433326666523 28.13105074985082 27.25542862904492 22.52408729443773 76-77 22.008513243594734 27.928014297171444 27.42674716208684 22.636725297146977 78-79 22.316395106932944 27.9038577052781 27.312538823173938 22.46720836461502 80-81 22.159131668875535 28.00254154725615 27.076668576353363 22.761658207514955 82-83 22.36093199834311 27.967024811393017 27.206249642555015 22.465793547708866 84-85 22.120735977118155 27.732268934398835 27.50598278109484 22.64101230738817 86-87 21.911391241737846 27.705262058263674 27.470777913464673 22.912568786533807 88-89 22.190952454783268 27.611307497592506 27.608133871483346 22.58960617614088 90-91 22.125941093668697 27.898224690886224 27.491141967207263 22.484692248237813 92-93 22.144957068527376 27.80454601203265 27.32473436636862 22.725762553071352 94-95 21.918454446128592 27.77388605044406 27.4489534485865 22.858706054840848 96-97 22.113562259767246 27.93058750761588 27.460791667644653 22.495058564972226 98-99 21.95152349030341 28.002475430001827 27.24351546763577 22.80248561205899 100-101 21.738114238978724 27.868024365586948 27.510725896403283 22.88313549903105 102-103 21.961902350184847 27.487552526338792 27.52996699737612 23.02057812610024 104-105 21.89319672613479 27.53997949040737 27.595881684378394 22.970942099079448 106-107 21.762284432743147 27.690561686467703 27.657436909200424 22.88971697158873 108-109 21.931569715278233 27.984461066382625 27.066551886695773 23.01741733164337 110-111 22.05266729985292 27.87868609774893 27.260387323536246 22.808259278861904 112-113 21.84889506570563 27.695673058327614 27.565785798089276 22.88964607787748 114-115 21.61523684341891 28.01406048600778 27.322077759790226 23.04862491078309 116-117 21.74106652878956 27.960214432400765 27.42162096402016 22.877098074789515 118-119 21.83257088338185 27.7966728134304 27.130534649432807 23.240221653754936 120-121 21.67558866865526 27.87003552822406 27.438057019047452 23.016318784073224 122-123 21.850117126987076 27.781840325138962 27.200238551605626 23.167803996268333 124-125 22.091185430430738 27.622378662682006 26.986265820587878 23.300170086299374 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 26.0 1 31.0 2 39.0 3 39.0 4 39.5 5 45.5 6 50.5 7 59.0 8 70.5 9 77.0 10 88.5 11 90.5 12 106.5 13 143.0 14 175.5 15 204.0 16 240.5 17 343.0 18 502.5 19 634.0 20 904.0 21 1411.0 22 2136.0 23 3125.5 24 4358.5 25 6126.0 26 8566.5 27 10952.0 28 13097.0 29 15666.0 30 19689.0 31 23908.5 32 27944.5 33 32317.5 34 36356.0 35 39736.0 36 43292.5 37 46829.0 38 48120.5 39 48483.0 40 48810.0 41 50866.0 42 52939.0 43 54216.0 44 57101.5 45 58940.0 46 58077.5 47 56323.5 48 56203.0 49 55132.5 50 52768.0 51 49992.0 52 47764.5 53 46581.5 54 44262.0 55 40722.0 56 36323.5 57 32485.0 58 29566.0 59 26410.0 60 22661.0 61 19351.5 62 16414.0 63 13140.0 64 10334.0 65 8386.5 66 6693.0 67 5360.5 68 4217.5 69 3061.0 70 2308.5 71 1839.0 72 1640.0 73 1454.0 74 1149.5 75 961.5 76 681.5 77 425.0 78 300.0 79 210.0 80 112.0 81 75.5 82 59.5 83 38.5 84 27.5 85 18.5 86 16.0 87 13.5 88 8.0 89 6.0 90 3.0 91 1.5 92 2.0 93 1.5 94 0.5 95 1.5 96 1.5 97 0.5 98 2.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.272847485082398E-4 2 2.6446718127572355E-4 3 6.611679531893089E-5 4 6.611679531893089E-4 5 0.0013884527016975487 6 0.001256219111059687 7 0.0011901023157407562 8 0.0019835038595679266 9 0.002512438222119374 10-11 0.0023140878361625813 12-13 0.0027438470057356322 14-15 0.004264533298071043 16-17 0.0036364237425411993 18-19 0.0036033653448817333 20-21 0.0045620588770062314 22-23 0.09134035273310302 24-25 0.003570306947222268 26-27 0.0026116134150977704 28-29 0.0022810294385031157 30-31 0.001785153473611134 32-33 0.0027107886080761662 34-35 0.0015206862923354105 36-37 7.934015438271706E-4 38-39 0.001619861485313807 40-41 0.0020496206548868577 42-43 0.0020165622572273926 44-45 0.0019504454619084614 46-47 0.0027438470057356322 48-49 5.950511578703781E-4 50-51 0.002082679052546323 52-53 0.0020165622572273926 54-55 0.0022149126431841846 56-57 0.0020165622572273926 58-59 0.0013553943040380831 60-61 0.0014215110993570142 62-63 0.0019835038595679266 64-65 0.0015868030876543412 66-67 5.619927602109125E-4 68-69 3.967007719135853E-4 70-71 9.586935321244979E-4 72-73 3.967007719135853E-4 74-75 4.958759648919816E-4 76-77 5.289343625514471E-4 78-79 5.619927602109125E-4 80-81 6.942263508487743E-4 82-83 2.9752557893518905E-4 84-85 6.281095555298435E-4 86-87 3.967007719135853E-4 88-89 6.281095555298435E-4 90-91 3.967007719135853E-4 92-93 4.2975916957305074E-4 94-95 4.958759648919816E-4 96-97 6.281095555298435E-4 98-99 6.942263508487743E-4 100-101 9.256351344650325E-4 102-103 0.0012892775087191522 104-105 7.934015438271706E-4 106-107 7.934015438271706E-4 108-109 0.0010578687251028942 110-111 0.0012231607134002215 112-113 6.281095555298435E-4 114-115 6.281095555298435E-4 116-117 0.001123985520421825 118-119 0.001719036678292203 120-121 0.0010248103274434288 122-123 9.256351344650325E-4 124-125 4.958759648919816E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1512475.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.9110590157216 #Duplication Level Percentage of deduplicated Percentage of total 1 51.41313047611422 16.920605712823896 2 14.638207409305902 9.635178158640795 3 10.474950353788156 10.342251278408273 4 7.448889596859027 9.806033804952886 5 4.935904992414449 8.122293025067341 6 3.2010824804574893 6.321060865711734 7 2.1541316943862356 4.962632872510723 8 1.3881060313743268 3.6547231614911655 9 0.9060708307480233 2.683777552485488 >10 3.148779021219984 17.69947220269586 >50 0.19702973186166983 4.482474710903884 >100 0.09251331943544644 5.1016184270302345 >500 0.001003385029303382 0.19905941247898798 >1k 2.0067700586067644E-4 0.0688188147986415 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.6529198829732722E-4 0.0 12-13 0.0 0.0 0.0 3.3058397659465444E-4 0.0 14-15 0.0 0.0 0.0 3.3058397659465444E-4 0.0 16-17 0.0 0.0 0.0 3.3058397659465444E-4 0.0 18-19 0.0 0.0 0.0 3.3058397659465444E-4 0.0 20-21 0.0 0.0 0.0 3.636423742541199E-4 0.0 22-23 0.0 0.0 0.0 3.9670077191358534E-4 0.0 24-25 0.0 0.0 0.0 4.6281756723251624E-4 0.0 26-27 0.0 0.0 0.0 5.289343625514471E-4 0.0 28-29 0.0 0.0 0.0 6.611679531893089E-4 0.0 30-31 0.0 0.0 0.0 0.0016198614853138067 0.0 32-33 0.0 0.0 0.0 0.004826526058281955 0.0 34-35 0.0 0.0 0.0 0.009487760128266583 0.0 36-37 1.9835038595679267E-4 0.0 0.0 0.01937222102844675 0.0 38-39 1.9835038595679267E-4 0.0 0.0 0.03679399659498504 0.0 40-41 1.9835038595679267E-4 0.0 0.0 0.055967867237475 0.0 42-43 1.9835038595679267E-4 0.0 0.0 0.07920792079207921 0.0 44-45 1.9835038595679267E-4 0.0 0.0 0.10955552984346849 0.0 46-47 1.9835038595679267E-4 0.0 0.0 0.14915949023950809 0.0 48-49 1.9835038595679267E-4 0.0 0.0 0.19021802013256417 0.0 50-51 1.9835038595679267E-4 0.0 0.0 0.2328633531132746 0.0 52-53 1.9835038595679267E-4 0.0 0.0 0.2809963801054563 0.0 54-55 1.9835038595679267E-4 0.0 0.0 0.3394766855650506 0.0 56-57 1.9835038595679267E-4 0.0 0.0 0.41141175887204745 0.0 58-59 1.9835038595679267E-4 0.0 0.0 0.4929007091026298 0.0 60-61 1.9835038595679267E-4 0.0 0.0 0.5781913750640506 0.0 62-63 1.9835038595679267E-4 0.0 0.0 0.665002727317807 0.0 64-65 1.9835038595679267E-4 1.3223359063786177E-4 0.0 0.7677482272434255 0.0 66-67 1.9835038595679267E-4 1.3223359063786177E-4 0.0 0.9006429858344767 0.0 68-69 1.9835038595679267E-4 1.3223359063786177E-4 0.0 1.0506289360154715 0.0 70-71 1.9835038595679267E-4 1.3223359063786177E-4 0.0 1.2137721284649334 0.0 72-73 1.9835038595679267E-4 1.3223359063786177E-4 0.0 1.3758243937916328 0.0 74-75 1.9835038595679267E-4 1.3223359063786177E-4 0.0 1.5612489462635746 0.0 76-77 1.9835038595679267E-4 1.3223359063786177E-4 0.0 1.7769549909915865 0.0 78-79 1.9835038595679267E-4 1.3223359063786177E-4 0.0 2.023934279905453 0.0 80-81 1.9835038595679267E-4 1.3223359063786177E-4 0.0 2.290616373824361 0.0 82-83 1.9835038595679267E-4 1.3223359063786177E-4 0.0 2.5670837534504702 0.0 84-85 1.9835038595679267E-4 1.3223359063786177E-4 0.0 2.8644440403973617 0.0 86-87 1.9835038595679267E-4 1.3223359063786177E-4 0.0 3.1977388056000926 0.0 88-89 1.9835038595679267E-4 1.3223359063786177E-4 0.0 3.5762905171986317 0.0 90-91 1.9835038595679267E-4 1.3223359063786177E-4 0.0 3.970544967685416 0.0 92-93 1.9835038595679267E-4 1.3223359063786177E-4 0.0 4.37851865320088 0.0 94-95 1.9835038595679267E-4 1.3223359063786177E-4 0.0 4.812344005686045 0.0 96-97 1.9835038595679267E-4 1.3223359063786177E-4 0.0 5.294566852344667 0.0 98-99 1.9835038595679267E-4 1.3223359063786177E-4 0.0 5.814707681118696 0.0 100-101 1.9835038595679267E-4 1.3223359063786177E-4 0.0 6.386915486206384 0.0 102-103 1.9835038595679267E-4 1.3223359063786177E-4 0.0 6.950461991107291 0.0 104-105 1.9835038595679267E-4 1.3223359063786177E-4 0.0 7.535397279293873 0.0 106-107 5.289343625514471E-4 1.3223359063786177E-4 0.0 8.160300170250748 0.0 108-109 6.942263508487743E-4 1.3223359063786177E-4 0.0 8.814625035124546 0.0 110-111 9.917519297839633E-4 1.3223359063786177E-4 0.0 9.505942246979288 0.0 112-113 9.917519297839633E-4 1.3223359063786177E-4 0.0 10.221061505148846 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2370 0.0 74.05398 1 GTATCAA 2935 0.0 59.595566 2 GTACATG 12815 0.0 52.87859 1 TACATGG 12960 0.0 51.368847 2 ACATGGG 12955 0.0 50.424274 3 CATGGGA 9030 0.0 47.305477 4 TCAACGC 3915 0.0 45.43747 5 ATCAACG 3935 0.0 45.20653 4 CAACGCA 3965 0.0 45.01454 6 AACGCAG 4165 0.0 42.710133 7 CATGGGG 3775 0.0 40.345745 4 TATCAAC 4500 0.0 39.530598 3 ATGGGAT 4030 0.0 39.121506 5 ATGGGAG 3220 0.0 38.800575 5 ACGCAGA 4985 0.0 34.968513 8 CGCAGAG 5095 0.0 34.096783 9 ATGGGAC 1750 0.0 30.93699 5 TACCTGG 1315 0.0 30.312641 2 GAGTACA 7610 0.0 29.942554 1 AGTACAT 7555 0.0 29.294304 2 >>END_MODULE