FastQCFastQC Report
Thu 26 May 2016
SRR936276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512475
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38450.2542190780012893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24280.1605315790343642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21850.14446519777186398No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34500.083.9177861
GTATCAA53150.054.3289072
GTACATG111000.051.0380861
TACATGG113500.049.3088072
ACATGGG112300.048.8814053
CATGGGA77750.047.094184
ATGGGAT32900.041.803115
TCAACGC74350.040.679475
ATCAACG74750.040.4617844
CAACGCA76300.039.8449136
AACGCAG78200.038.8007357
ATGGGAG30300.037.9233975
TATCAAC79950.037.5322463
CATGGGG33950.036.4767464
ACGCAGA92300.032.808978
CGCAGAG93900.032.2499289
GAGTACA69100.029.9149481
AGTACAT67900.029.7250392
ATGGGAC16100.027.7349835
CAGAGTA105500.026.05359
Produced by FastQC (version 0.11.5)